ATE321141T1 - Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines chymotrypsinhemmstoffes zum zellkulturmedium - Google Patents

Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines chymotrypsinhemmstoffes zum zellkulturmedium

Info

Publication number
ATE321141T1
ATE321141T1 AT03000391T AT03000391T ATE321141T1 AT E321141 T1 ATE321141 T1 AT E321141T1 AT 03000391 T AT03000391 T AT 03000391T AT 03000391 T AT03000391 T AT 03000391T AT E321141 T1 ATE321141 T1 AT E321141T1
Authority
AT
Austria
Prior art keywords
cell culture
culture medium
producing
inhibitor
recombinant polypeptide
Prior art date
Application number
AT03000391T
Other languages
English (en)
Inventor
Lars Adamson
Erik Walum
Johan Dixelius
Lie Kristina Lima
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SE9601855A external-priority patent/SE9601855D0/xx
Application filed filed Critical
Application granted granted Critical
Publication of ATE321141T1 publication Critical patent/ATE321141T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Diabetes (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Toxicology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
AT03000391T 1996-05-14 1997-05-13 Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines chymotrypsinhemmstoffes zum zellkulturmedium ATE321141T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE9601855A SE9601855D0 (sv) 1996-05-14 1996-05-14 Process for producing a protein
US1887496P 1996-05-29 1996-05-29

Publications (1)

Publication Number Publication Date
ATE321141T1 true ATE321141T1 (de) 2006-04-15

Family

ID=26662616

Family Applications (2)

Application Number Title Priority Date Filing Date
AT97923382T ATE334223T1 (de) 1996-05-14 1997-05-13 Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines hemmstoffes einer metallabhängigen protease oder chymotrypsins zum zellkulturmedium
AT03000391T ATE321141T1 (de) 1996-05-14 1997-05-13 Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines chymotrypsinhemmstoffes zum zellkulturmedium

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT97923382T ATE334223T1 (de) 1996-05-14 1997-05-13 Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines hemmstoffes einer metallabhängigen protease oder chymotrypsins zum zellkulturmedium

Country Status (13)

Country Link
US (1) US5851800A (de)
EP (1) EP0934424B1 (de)
JP (2) JP2000509994A (de)
AT (2) ATE334223T1 (de)
AU (1) AU716245B2 (de)
CA (1) CA2253287C (de)
DE (2) DE69735510T2 (de)
DK (1) DK0934424T3 (de)
ES (2) ES2270460T3 (de)
NO (2) NO325358B1 (de)
NZ (1) NZ332751A (de)
PT (2) PT934424E (de)
WO (1) WO1997043436A1 (de)

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US6475725B1 (en) * 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
EP1820516B1 (de) 1999-02-22 2013-07-24 University of Connecticut Neue albuminfreie Faktor VIII Formulierungen
AT409379B (de) 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
ATE330003T1 (de) * 1999-08-05 2006-07-15 Baxter Ag Rekombinanter stabiler zellklon, seine herstellung und verwendung
WO2001052891A1 (fr) * 2000-01-20 2001-07-26 Nippon Organon K. K. Inhibiteurs d'invasion plasmodiale dans des erythrocytes
DK1520008T3 (da) 2002-07-09 2012-10-15 Baxter Int Medium frit for dyreprotein til dyrkning af celler
EP1575998A4 (de) * 2002-12-23 2007-07-25 Bristol Myers Squibb Co Verfahren zur kultivierung vonsäugetierzellen zur proteinproduktion
TWI328614B (en) * 2002-12-23 2010-08-11 Bristol Myers Squibb Co Product quality enhancement in mammalian cell culture processes for protein production
MXPA05011486A (es) * 2003-04-25 2006-04-18 Immunex Corp Inductores de expresion de proteina recombinante.
US7255288B2 (en) * 2004-03-08 2007-08-14 Wan Shan Chan Aroma therapy for fountain
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
EP1707634A1 (de) * 2005-03-29 2006-10-04 Octapharma AG Verfahren zur Isolierung von rekombinant hergestellten Proteinen
ES2474573T3 (es) 2006-01-04 2014-07-09 Baxter International Inc Medio de cultivo celular sin oligop�ptidos
CA2679107C (en) * 2007-02-23 2018-01-16 Sk Chemicals Co., Ltd. Process for producing and purifying factor viii and its derivatives
WO2008135498A2 (en) * 2007-05-04 2008-11-13 Novo Nordisk A/S Prevention of protein degradation in mammalian cell cultures
EP1988101A1 (de) 2007-05-04 2008-11-05 Novo Nordisk A/S Verbesserung von Faktor-VIII-Polypeptid-Titern in Zellkulturen
EP2385825B1 (de) 2008-11-07 2018-10-10 University of Connecticut Faktor-viii-formulierungen
US9540426B2 (en) 2009-10-06 2017-01-10 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
WO2012045769A1 (en) 2010-10-05 2012-04-12 Novo Nordisk Health Care Ag Process for protein production
EP2906683B1 (de) 2012-10-15 2017-05-31 Bristol-Myers Squibb Company Säugerzellkulturverfahren zur proteinproduktion
KR102668200B1 (ko) 2015-10-28 2024-05-23 주식회사유한양행 지속형 fgf21 융합 단백질 및 이를 포함하는 약학적 조성물
KR102670157B1 (ko) 2015-10-28 2024-05-29 주식회사유한양행 이중 작용 단백질 및 이를 포함하는 약학적 조성물
HUE071618T2 (hu) 2016-11-10 2025-09-28 Yuhan Corp Fúziós fehérjéket tartalmazó gyógyszerkészítmény hepatitisz, májfibrózis és májzsugor megelõzésére vagy kezelésére
CN110709520B (zh) 2017-04-21 2024-04-05 株式会社柳韩洋行 用于产生双功能蛋白质及其衍生物的方法

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US5149787A (en) * 1984-05-22 1992-09-22 The Blood Center Research Foundation Method for maintaining intact, non-degraded factor VIII/von-Willebrand factor during blood processing
SE8501050D0 (sv) * 1985-03-05 1985-03-05 Kabivitrum Ab Biologically active fragments of human antihemophilic factor and method for preparation thereof
US5279939A (en) * 1986-08-18 1994-01-18 Smithkline Beecham Corporation Protein protease inhibitors from streptomyces
US4891356A (en) * 1987-07-15 1990-01-02 Brigham & Women's Hospital Proteinase inhibitors for treatment of gastrointestinal ulcer disease
JPH0387173A (ja) * 1987-09-10 1991-04-11 Teijin Ltd ヒト活性化天然型ファクター８ｃの製造方法及びそれに用いる形質転換体
JP2769170B2 (ja) * 1987-12-08 1998-06-25 ザイモジェネティクス，インコーポレイティド 真核細肪中での同時発現
CA1340740C (en) * 1987-12-08 1999-09-14 Eileen R. Mulvihill Co-expression in eukaryotic cells
WO1990002175A1 (en) * 1988-08-16 1990-03-08 Novo Nordisk A/S A method of producing polypeptides by culturing eukaryotic cells in the presence of protease inhibitors
DK457788D0 (da) * 1988-08-16 1988-08-16 Nordisk Gentofte Fremgangsmaade til fremstilling af et oensket polypeptid
GB8827305D0 (en) * 1988-11-23 1988-12-29 British Bio Technology Compounds
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
SE465222C5 (sv) * 1989-12-15 1998-02-10 Pharmacia & Upjohn Ab Ett rekombinant, humant faktor VIII-derivat och förfarande för dess framställning
ATE220421T1 (de) * 1990-02-26 2002-07-15 Univ Leland Stanford Junior Identifizierung und expression von insekten- steroidrezeptor-dns-sequenzen
US5189178A (en) * 1990-11-21 1993-02-23 Galardy Richard E Matrix metalloprotease inhibitors
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US5661034A (en) * 1991-07-18 1997-08-26 Fuji Yakuhin Kogyo Kabushiki Kaisha Serum-free medium for tissue culture containing tissue inhibitor of metalloproteinases and method for growing cells using the medium
EP0604580A1 (de) * 1991-09-19 1994-07-06 Genentech, Inc. Expression in E.Coli von Antikörperfragmenten mit mindestens einem cystein in freier -SH Form. Anwendung in der Herstellung bifunktioneller Antikörper.
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US5278289A (en) * 1991-11-12 1994-01-11 Johnson Alan J Antihemophilic factor stabilization
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WO1995005477A1 (en) * 1993-08-12 1995-02-23 University Of Maryland Thermostable alkaline metalloprotease produced by a hyphomonas, and preparation thereof
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US5543396A (en) * 1994-04-28 1996-08-06 Georgia Tech Research Corp. Proline phosphonate derivatives

Also Published As

Publication number Publication date
JP4896999B2 (ja) 2012-03-14
AU716245B2 (en) 2000-02-24
ES2260524T3 (es) 2006-11-01
DE69735510D1 (de) 2006-05-11
NO985297L (no) 1999-01-14
US5851800A (en) 1998-12-22
CA2253287A1 (en) 1997-11-20
EP0934424B1 (de) 2006-07-26
DE69735510T2 (de) 2006-08-24
AU2919797A (en) 1997-12-05
CA2253287C (en) 2008-02-19
WO1997043436A1 (en) 1997-11-20
NO325358B1 (no) 2008-04-07
NO985297D0 (no) 1998-11-13
NO20065027L (no) 1999-01-14
PT934424E (pt) 2006-12-29
JP2000509994A (ja) 2000-08-08
ES2270460T3 (es) 2007-04-01
ATE334223T1 (de) 2006-08-15
JP2009148271A (ja) 2009-07-09
DK0934424T3 (da) 2006-11-20
PT1318198E (pt) 2006-08-31
DE69736391T2 (de) 2007-08-16
NZ332751A (en) 2000-06-23
NO325633B1 (no) 2008-06-30
DE69736391D1 (de) 2006-09-07
EP0934424A1 (de) 1999-08-11

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