ATE334223T1 - Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines hemmstoffes einer metallabhängigen protease oder chymotrypsins zum zellkulturmedium - Google Patents

Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines hemmstoffes einer metallabhängigen protease oder chymotrypsins zum zellkulturmedium

Info

Publication number: ATE334223T1
Authority: AT; Austria
Prior art keywords: cell culture; culture medium; metal; chymotrypsin; producing
Prior art date: 1996-05-14

Application number

AT97923382T

Other languages

English (en)

Inventor

Lars Adamson

Erik Walum

Johan Dixelius

Lie Kristina Lima

Original Assignee

Biovitrum Ab

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1996-05-14

Filing date

1997-05-13

Publication date

2006-08-15

1996-05-14 Priority claimed from SE9601855A external-priority patent/SE9601855D0/xx

1997-05-13 Application filed by Biovitrum Ab filed Critical Biovitrum Ab

2006-08-15 Application granted granted Critical

2006-08-15 Publication of ATE334223T1 publication Critical patent/ATE334223T1/de

Links

239000006143 cell culture medium Substances 0.000 title abstract 4
238000004519 manufacturing process Methods 0.000 title abstract 3
229920001184 polypeptide Polymers 0.000 title abstract 3
102000004196 processed proteins & peptides Human genes 0.000 title abstract 3
108090000765 processed proteins & peptides Proteins 0.000 title abstract 3
CFYIUBWVKZQDOG-UHFFFAOYSA-N 4-[[2-[[2-[[1-(4-nitroanilino)-1-oxo-3-phenylpropan-2-yl]amino]-2-oxoethyl]amino]-2-oxoethyl]amino]-4-oxobutanoic acid Chemical compound C=1C=C([N+]([O-])=O)C=CC=1NC(=O)C(NC(=O)CNC(=O)CNC(=O)CCC(=O)O)CC1=CC=CC=C1 CFYIUBWVKZQDOG-UHFFFAOYSA-N 0.000 title 1
108090000317 Chymotrypsin Proteins 0.000 title 1
229940124158 Protease/peptidase inhibitor Drugs 0.000 title 1
229960002376 chymotrypsin Drugs 0.000 title 1
239000000137 peptide hydrolase inhibitor Substances 0.000 title 1
102000035195 Peptidases Human genes 0.000 abstract 3
108091005804 Peptidases Proteins 0.000 abstract 3
239000004365 Protease Substances 0.000 abstract 3
102100026735 Coagulation factor VIII Human genes 0.000 abstract 2
108010054218 Factor VIII Proteins 0.000 abstract 2
102000001690 Factor VIII Human genes 0.000 abstract 2
201000003542 Factor VIII deficiency Diseases 0.000 abstract 2
208000009292 Hemophilia A Diseases 0.000 abstract 2
101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 abstract 2
230000001419 dependent effect Effects 0.000 abstract 2
229960000301 factor viii Drugs 0.000 abstract 2
239000003112 inhibitor Substances 0.000 abstract 2
239000002184 metal Substances 0.000 abstract 2
238000000034 method Methods 0.000 abstract 2
108090000144 Human Proteins Proteins 0.000 abstract 1
102000003839 Human Proteins Human genes 0.000 abstract 1
238000004113 cell culture Methods 0.000 abstract 1
230000001627 detrimental effect Effects 0.000 abstract 1
239000003814 drug Substances 0.000 abstract 1
230000003248 secreting effect Effects 0.000 abstract 1
208000024891 symptom Diseases 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
General Engineering & Computer Science (AREA)
Hematology (AREA)
Microbiology (AREA)
Biophysics (AREA)
Biomedical Technology (AREA)
General Chemical & Material Sciences (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Chemical Kinetics & Catalysis (AREA)
Medicinal Chemistry (AREA)
Plant Pathology (AREA)
Animal Behavior & Ethology (AREA)
Physics & Mathematics (AREA)
Gastroenterology & Hepatology (AREA)
Diabetes (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Pharmacology & Pharmacy (AREA)
Toxicology (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Peptides Or Proteins (AREA)
Enzymes And Modification Thereof (AREA)

AT97923382T 1996-05-14 1997-05-13 Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines hemmstoffes einer metallabhängigen protease oder chymotrypsins zum zellkulturmedium ATE334223T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
SE9601855A SE9601855D0 (sv)	1996-05-14	1996-05-14	Process for producing a protein
US1887496P	1996-05-29	1996-05-29

Publications (1)

Publication Number	Publication Date
ATE334223T1 true ATE334223T1 (de)	2006-08-15

Family

ID=26662616

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
AT97923382T ATE334223T1 (de)	1996-05-14	1997-05-13	Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines hemmstoffes einer metallabhängigen protease oder chymotrypsins zum zellkulturmedium
AT03000391T ATE321141T1 (de)	1996-05-14	1997-05-13	Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines chymotrypsinhemmstoffes zum zellkulturmedium

Family Applications After (1)

Application Number	Title	Priority Date	Filing Date
AT03000391T ATE321141T1 (de)	1996-05-14	1997-05-13	Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines chymotrypsinhemmstoffes zum zellkulturmedium

Country Status (13)

Country	Link
US (1)	US5851800A (de)
EP (1)	EP0934424B1 (de)
JP (2)	JP2000509994A (de)
AT (2)	ATE334223T1 (de)
AU (1)	AU716245B2 (de)
CA (1)	CA2253287C (de)
DE (2)	DE69735510T2 (de)
DK (1)	DK0934424T3 (de)
ES (2)	ES2270460T3 (de)
NO (2)	NO325358B1 (de)
NZ (1)	NZ332751A (de)
PT (2)	PT934424E (de)
WO (1)	WO1997043436A1 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6475725B1 (en) *	1997-06-20	2002-11-05	Baxter Aktiengesellschaft	Recombinant cell clones having increased stability and methods of making and using the same
EP1820516B1 (de)	1999-02-22	2013-07-24	University of Connecticut	Neue albuminfreie Faktor VIII Formulierungen
AT409379B (de)	1999-06-02	2002-07-25	Baxter Ag	Medium zur protein- und serumfreien kultivierung von zellen
ATE330003T1 (de) *	1999-08-05	2006-07-15	Baxter Ag	Rekombinanter stabiler zellklon, seine herstellung und verwendung
WO2001052891A1 (fr) *	2000-01-20	2001-07-26	Nippon Organon K. K.	Inhibiteurs d'invasion plasmodiale dans des erythrocytes
DK1520008T3 (da)	2002-07-09	2012-10-15	Baxter Int	Medium frit for dyreprotein til dyrkning af celler
EP1575998A4 (de) *	2002-12-23	2007-07-25	Bristol Myers Squibb Co	Verfahren zur kultivierung vonsäugetierzellen zur proteinproduktion
TWI328614B (en) *	2002-12-23	2010-08-11	Bristol Myers Squibb Co	Product quality enhancement in mammalian cell culture processes for protein production
MXPA05011486A (es) *	2003-04-25	2006-04-18	Immunex Corp	Inductores de expresion de proteina recombinante.
US7255288B2 (en) *	2004-03-08	2007-08-14	Wan Shan Chan	Aroma therapy for fountain
US20060094104A1 (en)	2004-10-29	2006-05-04	Leopold Grillberger	Animal protein-free media for cultivation of cells
EP1707634A1 (de) *	2005-03-29	2006-10-04	Octapharma AG	Verfahren zur Isolierung von rekombinant hergestellten Proteinen
ES2474573T3 (es)	2006-01-04	2014-07-09	Baxter International Inc	Medio de cultivo celular sin oligop�ptidos
CA2679107C (en) *	2007-02-23	2018-01-16	Sk Chemicals Co., Ltd.	Process for producing and purifying factor viii and its derivatives
WO2008135498A2 (en) *	2007-05-04	2008-11-13	Novo Nordisk A/S	Prevention of protein degradation in mammalian cell cultures
EP1988101A1 (de)	2007-05-04	2008-11-05	Novo Nordisk A/S	Verbesserung von Faktor-VIII-Polypeptid-Titern in Zellkulturen
EP2385825B1 (de)	2008-11-07	2018-10-10	University of Connecticut	Faktor-viii-formulierungen
US9540426B2 (en)	2009-10-06	2017-01-10	Bristol-Myers Squibb Company	Mammalian cell culture processes for protein production
WO2012045769A1 (en)	2010-10-05	2012-04-12	Novo Nordisk Health Care Ag	Process for protein production
EP2906683B1 (de)	2012-10-15	2017-05-31	Bristol-Myers Squibb Company	Säugerzellkulturverfahren zur proteinproduktion
KR102668200B1 (ko)	2015-10-28	2024-05-23	주식회사유한양행	지속형 fgf21 융합 단백질 및 이를 포함하는 약학적 조성물
KR102670157B1 (ko)	2015-10-28	2024-05-29	주식회사유한양행	이중 작용 단백질 및 이를 포함하는 약학적 조성물
HUE071618T2 (hu)	2016-11-10	2025-09-28	Yuhan Corp	Fúziós fehérjéket tartalmazó gyógyszerkészítmény hepatitisz, májfibrózis és májzsugor megelõzésére vagy kezelésére
CN110709520B (zh)	2017-04-21	2024-04-05	株式会社柳韩洋行	用于产生双功能蛋白质及其衍生物的方法

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5149787A (en) *	1984-05-22	1992-09-22	The Blood Center Research Foundation	Method for maintaining intact, non-degraded factor VIII/von-Willebrand factor during blood processing
SE8501050D0 (sv) *	1985-03-05	1985-03-05	Kabivitrum Ab	Biologically active fragments of human antihemophilic factor and method for preparation thereof
US5279939A (en) *	1986-08-18	1994-01-18	Smithkline Beecham Corporation	Protein protease inhibitors from streptomyces
US4891356A (en) *	1987-07-15	1990-01-02	Brigham & Women's Hospital	Proteinase inhibitors for treatment of gastrointestinal ulcer disease
JPH0387173A (ja) *	1987-09-10	1991-04-11	Teijin Ltd	ヒト活性化天然型ファクター８ｃの製造方法及びそれに用いる形質転換体
JP2769170B2 (ja) *	1987-12-08	1998-06-25	ザイモジェネティクス，インコーポレイティド	真核細肪中での同時発現
CA1340740C (en) *	1987-12-08	1999-09-14	Eileen R. Mulvihill	Co-expression in eukaryotic cells
WO1990002175A1 (en) *	1988-08-16	1990-03-08	Novo Nordisk A/S	A method of producing polypeptides by culturing eukaryotic cells in the presence of protease inhibitors
DK457788D0 (da) *	1988-08-16	1988-08-16	Nordisk Gentofte	Fremgangsmaade til fremstilling af et oensket polypeptid
GB8827305D0 (en) *	1988-11-23	1988-12-29	British Bio Technology	Compounds
DK105489D0 (da) *	1989-03-03	1989-03-03	Novo Nordisk As	Polypeptid
SE465222C5 (sv) *	1989-12-15	1998-02-10	Pharmacia & Upjohn Ab	Ett rekombinant, humant faktor VIII-derivat och förfarande för dess framställning
ATE220421T1 (de) *	1990-02-26	2002-07-15	Univ Leland Stanford Junior	Identifizierung und expression von insekten- steroidrezeptor-dns-sequenzen
US5189178A (en) *	1990-11-21	1993-02-23	Galardy Richard E	Matrix metalloprotease inhibitors
US5304603A (en) *	1991-06-18	1994-04-19	The Population Council	Leydig cell stimulator
US5661034A (en) *	1991-07-18	1997-08-26	Fuji Yakuhin Kogyo Kabushiki Kaisha	Serum-free medium for tissue culture containing tissue inhibitor of metalloproteinases and method for growing cells using the medium
EP0604580A1 (de) *	1991-09-19	1994-07-06	Genentech, Inc.	Expression in E.Coli von Antikörperfragmenten mit mindestens einem cystein in freier -SH Form. Anwendung in der Herstellung bifunktioneller Antikörper.
CA2078721A1 (en) *	1991-09-24	1993-03-25	Hiroshi Yonemura	Process for preparing human coagulation factor viii protein complex
US5278289A (en) *	1991-11-12	1994-01-11	Johnson Alan J	Antihemophilic factor stabilization
US5296471A (en) *	1992-12-22	1994-03-22	Glycomed Incorporated	Method for controlling o-desulfation of heparin and compositions produced thereby
WO1995005477A1 (en) *	1993-08-12	1995-02-23	University Of Maryland	Thermostable alkaline metalloprotease produced by a hyphomonas, and preparation thereof
US5631159A (en) *	1993-09-22	1997-05-20	Creative Biomolecules, Inc.	Lipid-modified serum free media
US5543396A (en) *	1994-04-28	1996-08-06	Georgia Tech Research Corp.	Proline phosphonate derivatives

1997
- 1997-05-07 US US08/852,783 patent/US5851800A/en not_active Expired - Lifetime
- 1997-05-13 PT PT97923382T patent/PT934424E/pt unknown
- 1997-05-13 ES ES97923382T patent/ES2270460T3/es not_active Expired - Lifetime
- 1997-05-13 WO PCT/SE1997/000783 patent/WO1997043436A1/en not_active Ceased
- 1997-05-13 PT PT03000391T patent/PT1318198E/pt unknown
- 1997-05-13 EP EP97923382A patent/EP0934424B1/de not_active Expired - Lifetime
- 1997-05-13 NZ NZ332751A patent/NZ332751A/en not_active IP Right Cessation
- 1997-05-13 DK DK97923382T patent/DK0934424T3/da active
- 1997-05-13 DE DE69735510T patent/DE69735510T2/de not_active Expired - Lifetime
- 1997-05-13 DE DE69736391T patent/DE69736391T2/de not_active Expired - Lifetime
- 1997-05-13 AU AU29197/97A patent/AU716245B2/en not_active Ceased
- 1997-05-13 AT AT97923382T patent/ATE334223T1/de active
- 1997-05-13 JP JP09540800A patent/JP2000509994A/ja active Pending
- 1997-05-13 ES ES03000391T patent/ES2260524T3/es not_active Expired - Lifetime
- 1997-05-13 AT AT03000391T patent/ATE321141T1/de active
- 1997-05-13 CA CA002253287A patent/CA2253287C/en not_active Expired - Fee Related
1998
- 1998-11-13 NO NO19985297A patent/NO325358B1/no not_active IP Right Cessation
2006
- 2006-11-02 NO NO20065027A patent/NO325633B1/no not_active IP Right Cessation
2009
- 2009-01-07 JP JP2009001597A patent/JP4896999B2/ja not_active Expired - Fee Related

Also Published As

Publication number	Publication date
ATE321141T1 (de)	2006-04-15
JP4896999B2 (ja)	2012-03-14
AU716245B2 (en)	2000-02-24
ES2260524T3 (es)	2006-11-01
DE69735510D1 (de)	2006-05-11
NO985297L (no)	1999-01-14
US5851800A (en)	1998-12-22
CA2253287A1 (en)	1997-11-20
EP0934424B1 (de)	2006-07-26
DE69735510T2 (de)	2006-08-24
AU2919797A (en)	1997-12-05
CA2253287C (en)	2008-02-19
WO1997043436A1 (en)	1997-11-20
NO325358B1 (no)	2008-04-07
NO985297D0 (no)	1998-11-13
NO20065027L (no)	1999-01-14
PT934424E (pt)	2006-12-29
JP2000509994A (ja)	2000-08-08
ES2270460T3 (es)	2007-04-01
JP2009148271A (ja)	2009-07-09
DK0934424T3 (da)	2006-11-20
PT1318198E (pt)	2006-08-31
DE69736391T2 (de)	2007-08-16
NZ332751A (en)	2000-06-23
NO325633B1 (no)	2008-06-30
DE69736391D1 (de)	2006-09-07
EP0934424A1 (de)	1999-08-11

Legal Events

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2007-04-15

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Publication	Publication Date	Title
ATE321141T1 (de)	2006-04-15	Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines chymotrypsinhemmstoffes zum zellkulturmedium
ATE140968T1 (de)	1996-08-15	Kultur des wachstumsfaktors der vaskularen endothelialzellen und dns, die dafür kodiert
KR960703433A (ko)	1996-08-17	증가된 생물학적 활성을 갖는 끝이 잘린 케라틴세포성장인자(kgf) (a truncated keratinocyte growth factor(kgf) having increased biological activity)
DE60031456D1 (de)	2006-11-30	Rekombinante alpha-l-iduronisase, deren herstellungs- und aufreinigungsverfahren, und behandlung von durch diese hervorgerufene mangelerkrankungen
ES8602112A1 (es)	1985-11-16	Procedimiento para preparar un polipeptido ciclico
US4329430A (en)	1982-05-11	Enzyme mixture
DK0832186T3 (da)	2003-12-01	Erstatningsterapi til dental caries
US4307081A (en)	1981-12-22	Enzyme mixture
ATE92935T1 (de)	1993-08-15	Serinproteaseinhibitoren und isolierungsverfahren dazu.
DE69625682D1 (de)	2003-02-13	Verfahren zur behandlung von entzündungen und zusammensetzungen dafür
ATE291093T1 (de)	2005-04-15	Verfahren zur herstellung parthenokarper oder weiblich-steriler transgener pflanzen
JPH10310534A (ja)	1998-11-24	創傷治療用組成物及びペプチドの使用
Glyantsev et al.	1997	Crab collagenase in wound debridement
WO1999046368A3 (en)	2000-08-31	Carbohydrate-modifying enzymes for burn and wound debridement and methods for treatment
IL90947A (en)	1997-06-10	Method for production of hiv protease in transformed cells, dna and vectors comprising said dna used in said production
ATE101649T1 (de)	1994-03-15	Methode zur herstellung von natuerlichen, menschlichen wachstumshormon in reiner form.
AT404597B (de)	1998-12-28	Verfahren zur herstellung von proteinen
AU2007266775B2 (en)	2010-05-13	Chymotrypsin from Lucilia sericata larvae and its use for the treatment of wounds
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CA1077837A (en)	1980-05-20	Hydrolytic enzyme material
SE9300509D0 (sv)	1993-02-16	Peg treatment
IE890270L (en)	1989-07-28	Proteins derived from human alpha 2 plasmin inhibitor
ATE48531T1 (de)	1989-12-15	Verfahren zum sterilisieren von rekombinantem mikroorganismus.
FI102844B1 (fi)	1999-02-26	Menetelmä valmistaa parannettu terapeuttisesti aktiivinen kudosplasminogeenin aktivaattori
JPS6427472A (en)	1989-01-30	Production of human tissue plasminogen activator and cell strain using therein