ATE321867T1 - Zusammensetzungen und methoden zur verwendung von dna polymerasen - Google Patents

Zusammensetzungen und methoden zur verwendung von dna polymerasen

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Publication number
ATE321867T1
ATE321867T1 AT00973960T AT00973960T ATE321867T1 AT E321867 T1 ATE321867 T1 AT E321867T1 AT 00973960 T AT00973960 T AT 00973960T AT 00973960 T AT00973960 T AT 00973960T AT E321867 T1 ATE321867 T1 AT E321867T1
Authority
AT
Austria
Prior art keywords
compositions
methods
polymerase
dna polymerases
mutant
Prior art date
Application number
AT00973960T
Other languages
English (en)
Inventor
Joseph A Sorge
Holly H Hogrefe
Connie J Hansen
Original Assignee
Stratagene California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stratagene California filed Critical Stratagene California
Application granted granted Critical
Publication of ATE321867T1 publication Critical patent/ATE321867T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nanotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Mathematical Physics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Theoretical Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medical Informatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT00973960T 1999-10-29 2000-10-27 Zusammensetzungen und methoden zur verwendung von dna polymerasen ATE321867T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US16260099P 1999-10-29 1999-10-29

Publications (1)

Publication Number Publication Date
ATE321867T1 true ATE321867T1 (de) 2006-04-15

Family

ID=22586340

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00973960T ATE321867T1 (de) 1999-10-29 2000-10-27 Zusammensetzungen und methoden zur verwendung von dna polymerasen

Country Status (7)

Country Link
US (2) US6946273B1 (de)
EP (1) EP1226255B1 (de)
JP (1) JP4949585B2 (de)
AT (1) ATE321867T1 (de)
AU (1) AU1239901A (de)
DE (1) DE60027040T2 (de)
WO (1) WO2001032887A1 (de)

Families Citing this family (105)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050123940A1 (en) * 1999-10-29 2005-06-09 Stratagene California Compositions and methods for synthesizing cDNA
US20030228616A1 (en) * 1999-10-29 2003-12-11 Stratagene DNA polymerase mutants with reverse transcriptase activity
US20040009486A1 (en) * 1999-10-29 2004-01-15 Sorge Joseph A. Compositions and methods utilizing DNA polymerases
US8268605B2 (en) * 1999-10-29 2012-09-18 Agilent Technologies, Inc. Compositions and methods utilizing DNA polymerases
US20030180741A1 (en) 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
US6803201B2 (en) 2002-01-24 2004-10-12 Stratagene Compositions and methods for polynucleotide sequence determination
US8283148B2 (en) 2002-10-25 2012-10-09 Agilent Technologies, Inc. DNA polymerase compositions for quantitative PCR and methods thereof
CA2503038A1 (en) * 2002-10-25 2004-05-06 Stratagene California Dna polymerases with reduced base analog detection activity
US7960157B2 (en) * 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
EP2194123B1 (de) * 2003-03-25 2012-08-22 Stratagene California DNA-Polymerasefusionen und Verwendungen dafür
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
GB0324456D0 (en) * 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US20100216153A1 (en) 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
CA2568998A1 (en) * 2004-06-04 2005-12-15 Takara Bio Inc. Polypeptides having dna polymerase activity
US8623628B2 (en) 2005-05-10 2014-01-07 Illumina, Inc. Polymerases
KR100757280B1 (ko) 2005-11-01 2007-09-11 한국해양연구원 고호열성 신균주 ｋｃｔｃ 10859ｂｐ 및 이로부터 생산되는고호열성 아밀라아제
EP1914303A1 (de) * 2006-10-09 2008-04-23 Qiagen GmbH Thermus eggertssonii DNA Polymerasen
KR100777230B1 (ko) * 2006-11-30 2007-11-28 한국해양연구원 써모코커스 유래 돌연변이 ｄｎａ 중합효소들 및 그의유전자들
US9085762B2 (en) * 2006-12-29 2015-07-21 Agilent Technologies, Inc. Compositions and methods using split polymerases
US20090137405A1 (en) * 2007-11-16 2009-05-28 Christopher Bowman Detection of nucleic acid biomarkers using polymerization-based amplification
US7682809B2 (en) 2008-01-11 2010-03-23 Agilent Technologies, Inc. Direct ATP release sequencing
EP2644710B1 (de) 2008-04-30 2016-12-21 Integrated Dna Technologies, Inc. Rnase-h-basierte assays unter verwendung von modifizierten rna monomeren
US10227641B2 (en) 2008-04-30 2019-03-12 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2352818B1 (de) 2008-11-03 2016-02-10 Kapa Biosystems, Inc. Chimäre dna-polymerasen
WO2010068680A1 (en) 2008-12-10 2010-06-17 Mount Sinai School Of Medicine Of New York University Thelper cell type 17 lineage-specific adjuvants, compositions and methods
WO2011032097A1 (en) 2009-09-14 2011-03-17 Mount Sinai School Of Medicine Methods for characterizing antibody binding affinity and epitope diversity in food allergy
US8715987B2 (en) 2011-05-02 2014-05-06 New England Biolabs, Inc. Solubilized phospholipids for stabilizing nucleic acid polymerases
WO2012154934A1 (en) 2011-05-11 2012-11-15 New England Biolabs, Inc. Dna polymerase variants with reduced exonuclease activity and uses thereof
US8921044B2 (en) 2011-05-11 2014-12-30 New England Biolabs, Inc. DNA polymerase variants with reduced exonuclease activity and uses thereof
US20140235474A1 (en) 2011-06-24 2014-08-21 Sequenom, Inc. Methods and processes for non invasive assessment of a genetic variation
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9367663B2 (en) 2011-10-06 2016-06-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
US20140242588A1 (en) 2011-10-06 2014-08-28 Sequenom, Inc Methods and processes for non-invasive assessment of genetic variations
EP2805280B1 (de) 2012-01-20 2022-10-05 Sequenom, Inc. Diagnostische verfahren zur evaluierung von versuchsbedingungen
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
KR20210072137A (ko) 2012-06-14 2021-06-16 라이프 테크놀로지스 코포레이션 폴리머라제 연쇄 반응 (pcr)을 위한 신규 조성물, 방법 및 키트
US10497461B2 (en) 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
JP6478444B2 (ja) 2012-09-28 2019-03-06 東洋紡株式会社 改変された耐熱性ｄｎａポリメラーゼ
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20130309666A1 (en) 2013-01-25 2013-11-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP4187543A1 (de) 2013-04-03 2023-05-31 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
US10699800B2 (en) 2013-05-24 2020-06-30 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
KR102299305B1 (ko) 2013-06-21 2021-09-06 시쿼넘, 인코포레이티드 유전적 변이의 비침습 평가를 위한 방법 및 프로세스
RS59991B1 (sr) 2013-08-08 2020-04-30 Scripps Research Inst Metoda enzimskog in vitro obeležavanja specifičnog za položaj nukleinskih kiselina uvođenjem neprirodnih nukleotida
DK3053071T3 (da) 2013-10-04 2024-01-22 Sequenom Inc Fremgangsmåder og processer til ikke-invasiv bedømmelse af genetiske variationer
EP3495496B1 (de) 2013-10-07 2020-11-25 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung chromosomaler veränderungen
JP2016536995A (ja) 2013-11-14 2016-12-01 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド 増大した鋳型識別活性を有するｄｎａポリメラーゼ突然変異体
TWI638047B (zh) 2014-04-09 2018-10-11 史基普研究協會 藉由核酸三磷酸酯轉運子將非天然或經修飾的核苷三磷酸酯輸入至細胞中
EP3175000B1 (de) 2014-07-30 2020-07-29 Sequenom, Inc. Verfahren und prozesse zur nicht-invasiven beurteilung genetischer variationen
CN114517189A (zh) 2014-11-14 2022-05-20 亿明达股份有限公司 聚合酶
WO2017042040A1 (en) * 2015-09-09 2017-03-16 Qiagen Gmbh Polymerase enzyme
WO2017106767A1 (en) 2015-12-18 2017-06-22 The Scripps Research Institute Production of unnatural nucleotides using a crispr/cas9 system
WO2017121836A1 (en) 2016-01-15 2017-07-20 Thermo Fisher Scientific Baltics Uab Thermophilic dna polymerase mutants
EP3423575B1 (de) * 2016-02-29 2021-06-16 Genia Technologies, Inc. Exonukleasearme polymerasen
EP3458597B1 (de) 2016-05-18 2022-09-07 Roche Diagnostics GmbH Quantitative echtzeit-pcr-amplifikation unter verwendung einer elektrobenetzungsbasierten vorrichtung
ES2898088T3 (es) 2016-06-01 2022-03-03 Hoffmann La Roche Inmuno-PETE
EP4163293A1 (de) 2016-06-24 2023-04-12 The Scripps Research Institute Neuartiger nukleosid-triphosphat-transporter und verwendungen davon
WO2018013710A1 (en) 2016-07-12 2018-01-18 F. Hoffman-La Roche Ag Primer extension target enrichment
US11200963B2 (en) 2016-07-27 2021-12-14 Sequenom, Inc. Genetic copy number alteration classifications
WO2018140521A1 (en) 2017-01-24 2018-08-02 Sequenom, Inc. Methods and processes for assessment of genetic variations
EP3580351A1 (de) 2017-02-13 2019-12-18 Qiagen Sciences, LLC Polymeraseenzym aus 9°n
CN110352251A (zh) 2017-03-08 2019-10-18 豪夫迈·罗氏有限公司 引物延伸靶标富集及对其的包括同时富集dna和rna的改进
US12297491B2 (en) 2017-03-08 2025-05-13 Roche Sequencing Solutions, Inc. Primer extension target enrichment and improvements thereto including simultaneous enrichment of DNA and RNA
PT3596233T (pt) 2017-03-17 2022-08-22 Sequenom Inc Métodos e processos para avaliação de mosaicismo genético
WO2018191857A1 (zh) * 2017-04-18 2018-10-25 深圳华大智造科技有限公司 Phi29 DNA聚合酶及其编码基因与应用
US11618891B2 (en) 2017-06-26 2023-04-04 Thermo Fisher Scientific Baltics Uab Thermophilic DNA polymerase mutants
SG11202000167SA (en) 2017-07-11 2020-02-27 Synthorx Inc Incorporation of unnatural nucleotides and methods thereof
MA49716A (fr) 2017-07-11 2021-04-07 Scripps Research Inst Incorporation de nucléotides non naturels et procédés d'utilisationin vivo
KR20250145137A (ko) 2017-08-03 2025-10-13 신톡스, 인크. 자가면역 질환의 치료를 위한 사이토카인 접합체
IL275694B2 (en) 2017-12-29 2025-08-01 Scripps Research Inst Unnatural base pair compositions and methods of use
CA3103719A1 (en) 2018-10-31 2020-05-07 Illumina, Inc. Polymerases, compositions, and methods of use
CA3103739A1 (en) 2018-12-05 2020-06-11 Illumina, Inc. Polymerases, compositions, and methods of use
US20200246467A1 (en) 2019-02-06 2020-08-06 Synthorx, Inc. Il-2 conjugates and methods of use thereof
BR112021025130A2 (pt) 2019-06-14 2022-03-15 Scripps Research Inst Reagentes e métodos para replicação, transcrição e tradução em organismos semissintéticos
WO2021032660A1 (en) 2019-08-19 2021-02-25 F. Hoffmann-La Roche Ag Single tube preparation of dna and rna for sequencing
US20220372566A1 (en) 2019-09-20 2022-11-24 Roche Sequencing Solutions, Inc. Immune repertoire profiling by primer extension target enrichment
EP4055155A1 (de) * 2019-11-08 2022-09-14 Pacific Biosciences of California, Inc. Modifizierte polymerasen zur verbesserten sequenzierung durch bindung
US11591629B2 (en) * 2019-12-23 2023-02-28 Cheng-Yao Chen Method and kit for template-independent nucleic acid synthesis
US12590328B2 (en) 2020-07-08 2026-03-31 Roche Sequencing Solutions, Inc. Targeted depletion of non-target library molecules using poison primers during target capture of next-generation sequencing libraries
US12378596B2 (en) 2020-12-03 2025-08-05 Roche Sequencing Solutions, Inc. Whole transcriptome analysis in single cells
EP4267762B1 (de) 2020-12-24 2025-09-17 Integrated DNA Technologies, Inc. Rnase-h2-mutanten zur reduzierung von primerdimeren und off-target-amplifikation in der rhpcr-basierten amplikonsequenzierung mit high-fidelity-dna-polymerasen
CN112779238B (zh) * 2020-12-28 2024-01-30 广州达安基因股份有限公司 用于丙肝病毒检测的dna聚合酶混合物
EP4294941B1 (de) 2021-02-18 2025-03-26 F. Hoffmann-La Roche AG Struktur zur verhinderung des einfädelns von nukleinsäuretemplates durch eine nanopore während der sequenzierung
US20240158839A1 (en) 2021-03-15 2024-05-16 President And Fellows Of Harvard College Methods for in situ sequencing
WO2022194764A1 (en) 2021-03-15 2022-09-22 F. Hoffmann-La Roche Ag Targeted next-generation sequencing via anchored primer extension
EP4314337A1 (de) 2021-04-01 2024-02-07 F. Hoffmann-La Roche AG Immunzellenzählung von sars-cov-2-patienten auf der basis von immunrepertoiresequenzierung
IL309440A (en) * 2021-06-18 2024-02-01 Element Biosciences Inc Engineered polymerases
US12077789B2 (en) 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
TWI869710B (zh) * 2021-09-29 2025-01-11 呈堯 陳 用於無模板ｒｎａ合成的核酸聚合酶變體、試劑套組及方法
CN114015672B (zh) * 2021-12-06 2022-05-31 江南大学 一种Pfu DNA聚合酶
US12163163B2 (en) * 2022-02-18 2024-12-10 Element Biosciences, Inc. Engineered polymerases with reduced sequence-specific errors
JP2025521678A (ja) 2022-06-30 2025-07-10 カパ バイオシステムズ，インコーポレイティド 古細菌ヒストン様タンパク質を使用したタグメンテーション配列決定ライブラリーインサートサイズの制御
CN121241132A (zh) * 2023-06-02 2025-12-30 武汉华大智造科技有限公司 用于测序的dna聚合酶突变体
WO2024256358A2 (en) 2023-06-14 2024-12-19 F. Hoffmann-La Roche Ag Accelerated marking of 5-formyl cytosine and use in nucleic acid methylation sequencing
WO2025024676A1 (en) 2023-07-26 2025-01-30 Caribou Biosciences, Inc. In vitro validation methods for cd19-targeting cell therapies
WO2025193609A1 (en) 2024-03-11 2025-09-18 Caribou Biosciences, Inc. Methods and compositions for cost-effective assessment of nucleic acid transcripts and isoforms in cells
WO2025207517A2 (en) 2024-03-25 2025-10-02 Synthorx, Inc. Synthetic trna synthetases and cells comprising synthetic molecules for production of polypeptides
WO2025210056A1 (en) 2024-04-03 2025-10-09 Roche Sequencing Solutions, Inc. In vitro amplification of dna methylation patterns
WO2025240460A2 (en) 2024-05-14 2025-11-20 Roche Molecular Systems, Inc. Assay for detection of mutations conferring resistance to treatment with an immunotherapeutic agent
WO2026082489A1 (en) 2024-10-18 2026-04-23 F. Hoffmann-La Roche Ag Universal pcr to boost molecule recovery in cell free dna library preparation and target enrichment workflows
WO2026087220A1 (en) 2024-10-22 2026-04-30 F. Hoffmann-La Roche Ag Preparation of dna libraries for next generation sequencing using y-adapters modified with phosphorothioate bonds
WO2026093331A1 (en) 2024-10-28 2026-05-07 F. Hoffmann-La Roche Ag Hairpin adapter constructs and primers to generate next-generation sequencing library molecules

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5674679A (en) 1991-09-27 1997-10-07 Amersham Life Science, Inc. DNA cycle sequencing
US5547859A (en) * 1993-08-02 1996-08-20 Goodman; Myron F. Chain-terminating nucleotides for DNA sequencing methods
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
EP0655506B1 (de) 1994-10-17 1996-09-18 President And Fellows Of Harvard College DNS Polymerase mit veränderter Nukleotid-Bindungstelle
CA2222744C (en) * 1995-05-31 2008-03-25 Amersham Life Science, Inc. Thermostable dna polymerases
EP0910664A1 (de) * 1996-04-15 1999-04-28 University Of Southern California Synthese von fluorophor-markierter dns
US5882904A (en) * 1997-08-04 1999-03-16 Amersham Pharmacia Biotech Inc. Thermococcus barossii DNA polymerase mutants

Also Published As

Publication number Publication date
JP2004500052A (ja) 2004-01-08
US20050158730A1 (en) 2005-07-21
US6946273B1 (en) 2005-09-20
JP4949585B2 (ja) 2012-06-13
DE60027040D1 (de) 2006-05-18
DE60027040T2 (de) 2006-11-23
WO2001032887A1 (en) 2001-05-10
AU1239901A (en) 2001-05-14
EP1226255A1 (de) 2002-07-31
EP1226255B1 (de) 2006-03-29

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