ATE322506T1 - Verfahren zur reinigung von in bakterien exprimierten proteinen - Google Patents
Verfahren zur reinigung von in bakterien exprimierten proteinenInfo
- Publication number
- ATE322506T1 ATE322506T1 AT03100064T AT03100064T ATE322506T1 AT E322506 T1 ATE322506 T1 AT E322506T1 AT 03100064 T AT03100064 T AT 03100064T AT 03100064 T AT03100064 T AT 03100064T AT E322506 T1 ATE322506 T1 AT E322506T1
- Authority
- AT
- Austria
- Prior art keywords
- proteins expressed
- bacteria
- purifying proteins
- denaturation
- purification
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 241000894006 Bacteria Species 0.000 title 1
- 238000004925 denaturation Methods 0.000 abstract 1
- 230000036425 denaturation Effects 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 210000001236 prokaryotic cell Anatomy 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 238000004153 renaturation Methods 0.000 abstract 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/521—Chemokines
- C07K14/523—Beta-chemokines, e.g. RANTES, I-309/TCA-3, MIP-1alpha, MIP-1beta/ACT-2/LD78/SCIF, MCP-1/MCAF, MCP-2, MCP-3, LDCF-1, LDCF-2
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2004/050025 WO2005068500A1 (en) | 2004-01-19 | 2004-01-19 | Process for the purification of bacterially expressed proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE322506T1 true ATE322506T1 (de) | 2006-04-15 |
Family
ID=37055134
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03100064T ATE322506T1 (de) | 2004-01-19 | 2003-01-15 | Verfahren zur reinigung von in bakterien exprimierten proteinen |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP1439191B1 (de) |
| JP (1) | JP4865569B2 (de) |
| AT (1) | ATE322506T1 (de) |
| AU (1) | AU2004313698B2 (de) |
| CA (1) | CA2552790C (de) |
| CY (1) | CY1105285T1 (de) |
| DE (1) | DE60304435T2 (de) |
| DK (1) | DK1439191T3 (de) |
| ES (1) | ES2257642T3 (de) |
| IL (1) | IL176763A (de) |
| NO (1) | NO20063280L (de) |
| PT (1) | PT1439191E (de) |
| SI (1) | SI1439191T1 (de) |
| WO (1) | WO2005068500A1 (de) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE322506T1 (de) * | 2004-01-19 | 2006-04-15 | Ares Trading Sa | Verfahren zur reinigung von in bakterien exprimierten proteinen |
| WO2005077973A1 (en) * | 2004-02-11 | 2005-08-25 | Virchow Biotech Private Limited | Purification of recombinant human proteins |
| JP5637857B2 (ja) * | 2008-10-28 | 2014-12-10 | 学校法人関西学院 | リフォールディング剤および蛋白質のリフォールディング方法 |
| KR20240064367A (ko) * | 2022-11-04 | 2024-05-13 | 주식회사 대웅제약 | TGF-β3 단백질의 정제방법 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5912327A (en) * | 1996-09-30 | 1999-06-15 | Human Genome Sciences, Inc. | Method of purifying chemokines from inclusion bodies |
| EP1094863A4 (de) * | 1998-07-06 | 2005-02-02 | Cambridge Neuroscience Inc | Verfahren zur behandlung von neurologischen verletzungen und erkrankungen |
| GB0006537D0 (en) * | 2000-03-17 | 2000-05-10 | Angeletti P Ist Richerche Bio | Assays and screening methods |
| UA77950C2 (en) * | 2000-10-04 | 2007-02-15 | Applied Research Systems | Use of mutants of cc chemokines for treatment of multiple sclerosis |
| WO2003051921A1 (en) * | 2001-12-17 | 2003-06-26 | Applied Research Systems Ars Holding N.V. | Chemokine mutants acting as chemokine antagonists |
| SI21102A (sl) * | 2001-12-19 | 2003-06-30 | LEK, tovarna farmacevtskih in kemi�nih izdelkov, d.d. | Postopek za izolacijo biološko aktivnega granulocitne kolonije stimulirajočega dejavnika |
| CA2490646A1 (en) * | 2002-06-25 | 2003-12-31 | Pharmacia Corporation | Arylsulfonylhydroxamic acid and amide derivatives and their use as protease inhibitors |
| ATE322506T1 (de) * | 2004-01-19 | 2006-04-15 | Ares Trading Sa | Verfahren zur reinigung von in bakterien exprimierten proteinen |
-
2003
- 2003-01-15 AT AT03100064T patent/ATE322506T1/de active
- 2003-01-15 PT PT03100064T patent/PT1439191E/pt unknown
- 2003-01-15 DE DE60304435T patent/DE60304435T2/de not_active Expired - Lifetime
- 2003-01-15 ES ES03100064T patent/ES2257642T3/es not_active Expired - Lifetime
- 2003-01-15 EP EP03100064A patent/EP1439191B1/de not_active Expired - Lifetime
- 2003-01-15 SI SI200330234T patent/SI1439191T1/sl unknown
- 2003-01-15 DK DK03100064T patent/DK1439191T3/da active
-
2004
- 2004-01-19 JP JP2006548186A patent/JP4865569B2/ja not_active Expired - Fee Related
- 2004-01-19 AU AU2004313698A patent/AU2004313698B2/en not_active Ceased
- 2004-01-19 CA CA2552790A patent/CA2552790C/en not_active Expired - Fee Related
- 2004-01-19 WO PCT/EP2004/050025 patent/WO2005068500A1/en not_active Ceased
-
2006
- 2006-05-15 CY CY20061100623T patent/CY1105285T1/el unknown
- 2006-07-10 IL IL176763A patent/IL176763A/en not_active IP Right Cessation
- 2006-07-14 NO NO20063280A patent/NO20063280L/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| EP1439191A1 (de) | 2004-07-21 |
| CY1105285T1 (el) | 2010-03-03 |
| AU2004313698B2 (en) | 2010-04-01 |
| AU2004313698A1 (en) | 2005-07-28 |
| EP1439191B1 (de) | 2006-04-05 |
| DE60304435D1 (de) | 2006-05-18 |
| SI1439191T1 (sl) | 2006-06-30 |
| CA2552790A1 (en) | 2005-07-28 |
| JP2007537150A (ja) | 2007-12-20 |
| ES2257642T3 (es) | 2006-08-01 |
| JP4865569B2 (ja) | 2012-02-01 |
| IL176763A0 (en) | 2006-10-31 |
| DK1439191T3 (da) | 2006-08-14 |
| NO20063280L (no) | 2006-10-16 |
| WO2005068500A1 (en) | 2005-07-28 |
| PT1439191E (pt) | 2006-06-30 |
| CA2552790C (en) | 2011-08-16 |
| DE60304435T2 (de) | 2006-08-24 |
| IL176763A (en) | 2011-02-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE384742T1 (de) | Verfahren zur reinigung von preproinsulin | |
| ATE278785T1 (de) | Neues verfahren zur gewinnung und reinigung von fusionsproteinen | |
| WO2003066662A3 (en) | Protein purification | |
| WO2004024866A3 (en) | Protein purification | |
| WO2003102132A3 (en) | Non-affinity purification of proteins | |
| ATE278706T1 (de) | Verfahren zur konzentrierung von unlöslichen protein mittels filtration mit schwingenden membranen | |
| WO2002083710A3 (en) | Removal of bacterial endotoxin in a protein solution by immobilized metal affinity chromatography | |
| UA94206C2 (ru) | Бактериальные факторы вирулентности и варианты их применения | |
| ATE449104T1 (de) | Verfahren zur reinigung von fibrinogen | |
| DE602004030850D1 (de) | Vorrichtung und Verfahren zur Trennung und Reinigung von Nucleinsäuren | |
| ATE417056T1 (de) | Verfahren zur proteinreinigung | |
| DE50208601D1 (de) | Verfahren zur aufreinigung von bakterienzellen und zellbestandteilen | |
| ATE416619T1 (de) | Verfahren zur dearomatisierung von molkeprotein | |
| DE602004004334D1 (de) | Bearbeitungsverfahren zur Bearbeitung von gewölbten Oberflächen | |
| DK1105508T3 (da) | Fremgangsmåde til oprensning af biomolekyle- eller proteinkomplekser | |
| ATE322506T1 (de) | Verfahren zur reinigung von in bakterien exprimierten proteinen | |
| IL169804A (en) | 254p1d6b proteins, nucleic acids encoding the same, processes for producing the same, antibodies binding thereto and uses thereof | |
| WO2002012292A3 (en) | Method of polypeptide renaturation | |
| DE602005006550D1 (de) | Verfahren zur aufreinigung von mesotrion | |
| ATE278699T1 (de) | Verfahren zur aufreinigung von nukleinsäuren sowie anionenaustauscher zur durchführung dieses verfahrens | |
| ATE350387T1 (de) | Verfahren zur reinigung von molekülen unter verwendung von unverzweigten alkyldiolen mit einständigen hydroxylgruppen | |
| ATE487791T1 (de) | Verfahren und formulierung zur extraktion von nukleinsäuren aus beliebigen komplexen ausgangsmaterialien | |
| WO2003052136A3 (de) | Sequenzierung über lochmembranen | |
| ATE322478T1 (de) | Verfahren zur reinigung von caprolactam | |
| IS8375A (is) | Aðferð til að hreinsa prótín framleidd í plöntum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 1439191 Country of ref document: EP |