ATE323759T1 - Rückgewinnung eines proteins bei hohem ph-wert - Google Patents
Rückgewinnung eines proteins bei hohem ph-wertInfo
- Publication number
- ATE323759T1 ATE323759T1 AT00901052T AT00901052T ATE323759T1 AT E323759 T1 ATE323759 T1 AT E323759T1 AT 00901052 T AT00901052 T AT 00901052T AT 00901052 T AT00901052 T AT 00901052T AT E323759 T1 ATE323759 T1 AT E323759T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- recovery
- solution
- state
- glycosidase
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 238000011084 recovery Methods 0.000 title 1
- 102000005744 Glycoside Hydrolases Human genes 0.000 abstract 1
- 108010031186 Glycoside Hydrolases Proteins 0.000 abstract 1
- 102000035195 Peptidases Human genes 0.000 abstract 1
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 235000019833 protease Nutrition 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA199900085 | 1999-01-25 | ||
| DKPA199901491 | 1999-10-18 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE323759T1 true ATE323759T1 (de) | 2006-05-15 |
Family
ID=26063274
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00901052T ATE323759T1 (de) | 1999-01-25 | 2000-01-24 | Rückgewinnung eines proteins bei hohem ph-wert |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US6316240B1 (de) |
| EP (1) | EP1149160B1 (de) |
| JP (1) | JP2002534975A (de) |
| CN (1) | CN1181197C (de) |
| AT (1) | ATE323759T1 (de) |
| AU (1) | AU2093100A (de) |
| DE (1) | DE60027406T2 (de) |
| DK (1) | DK1149160T3 (de) |
| WO (1) | WO2000043502A1 (de) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100491525C (zh) | 2000-07-28 | 2009-05-27 | 汉高两合股份公司 | 从芽孢杆菌a7-7(dsm 12368)中提取的新型淀粉分解酶以及含有该新型淀粉分解酶的洗涤剂和清洗剂 |
| RU2325819C2 (ru) * | 2006-03-16 | 2008-06-10 | Вячеслав Юрьевич Елупов | Йодированные крупы |
| WO2008110498A1 (en) * | 2007-03-15 | 2008-09-18 | Novozymes A/S | Solubilization of protease crystals in fermentation broth |
| EP2285823B2 (de) * | 2008-06-09 | 2024-03-06 | Danisco US Inc. | Rückgewinnung von unlöslichem enzym aus einer fermentationsbrühe und formulierung des unlöslichen enzyms |
| GB0908770D0 (en) | 2009-04-24 | 2009-07-01 | Danisco | Method |
| EP2914611B1 (de) * | 2012-11-01 | 2018-08-29 | Novozymes A/S | Verfahren zum entfernen von dna |
| DK3137483T3 (en) * | 2014-04-30 | 2019-04-23 | Novozymes As | Method of reducing the DNA content of a fermentation broth |
| CA3005292A1 (en) | 2015-12-09 | 2017-06-15 | Basf Se | Method of purifying a protein from fermentation solids under desorbing conditions |
| US20200109388A1 (en) * | 2017-04-03 | 2020-04-09 | Novozymes A/S | Recovery Process |
| US11732250B2 (en) | 2018-04-26 | 2023-08-22 | Basf Se | Lipase enzymes |
| WO2019211143A1 (en) | 2018-05-03 | 2019-11-07 | Basf Se | Amylase enzymes |
| CN114096676A (zh) | 2019-02-20 | 2022-02-25 | 巴斯夫欧洲公司 | 用确定成分培养基和镁补料的芽孢杆菌工业发酵工艺 |
| WO2020169564A1 (en) | 2019-02-20 | 2020-08-27 | Basf Se | Industrial fermentation process for bacillus using defined medium and trace element feed |
| EP3947665A2 (de) | 2019-03-25 | 2022-02-09 | Basf Se | Amylaseenzyme |
| US12606809B2 (en) | 2019-03-25 | 2026-04-21 | Basf Se | Amylase enzymes |
| WO2020193532A1 (en) | 2019-03-25 | 2020-10-01 | Basf Se | Cleaning composition having amylase enzymes |
| US12146171B2 (en) | 2019-06-13 | 2024-11-19 | Basf Se | Method of recovering a protein from fermentation broth using a divalent cation |
| US20220340865A1 (en) | 2019-07-02 | 2022-10-27 | Basf Se | Method for preparing a fermentation medium |
| WO2021004830A1 (en) | 2019-07-05 | 2021-01-14 | Basf Se | Industrial fermentation process for microbial cells using a fed-batch pre-culture |
| CN114364795A (zh) | 2019-08-22 | 2022-04-15 | 巴斯夫欧洲公司 | 淀粉酶变体 |
| EP4047088A1 (de) | 2021-02-22 | 2022-08-24 | Basf Se | Amylasevarianten |
| MX2023009756A (es) | 2021-02-22 | 2023-09-04 | Basf Se | Variantes de amilasa. |
| US20260062690A1 (en) | 2022-08-02 | 2026-03-05 | Basf Se | Stabilized protein production process using bacillus host cells |
| JP2025526738A (ja) | 2022-08-11 | 2025-08-15 | ビーエーエスエフ ソシエタス・ヨーロピア | アミラーゼバリアント |
| JP2025526654A (ja) | 2022-08-11 | 2025-08-15 | ビーエーエスエフ ソシエタス・ヨーロピア | プロテアーゼ、マンナナーゼ及び／又はセルラーゼを含む酵素組成物 |
| JP2025526736A (ja) | 2022-08-11 | 2025-08-15 | ビーエーエスエフ ソシエタス・ヨーロピア | アミラーゼバリアント |
| WO2024033133A2 (en) | 2022-08-11 | 2024-02-15 | Basf Se | Enzyme compositions comprising an amylase |
| WO2024094735A1 (en) | 2022-11-04 | 2024-05-10 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
| EP4612285A1 (de) | 2022-11-04 | 2025-09-10 | Basf Se | Polypeptide mit proteaseaktivität zur verwendung in waschmittelzusammensetzungen |
| EP4612286A1 (de) | 2022-11-04 | 2025-09-10 | Basf Se | Polypeptide mit proteaseaktivität zur verwendung in waschmittelzusammensetzungen |
| EP4389864A1 (de) | 2022-12-20 | 2024-06-26 | Basf Se | Cutinasen |
| WO2025031865A1 (en) | 2023-08-08 | 2025-02-13 | Basf Se | Improved bacillus cell with inactivated metalloprotease |
| WO2025202374A1 (en) | 2024-03-27 | 2025-10-02 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
| WO2025202369A1 (en) | 2024-03-27 | 2025-10-02 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
| WO2025202372A1 (en) | 2024-03-27 | 2025-10-02 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
| WO2025202379A1 (en) | 2024-03-27 | 2025-10-02 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
| EP4624572A1 (de) | 2024-03-27 | 2025-10-01 | Basf Se | Polypeptide mit proteaseaktivität zur verwendung in waschmittelzusammensetzungen |
| WO2025202370A1 (en) | 2024-03-27 | 2025-10-02 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IE55163B1 (en) * | 1982-06-07 | 1990-06-20 | Celltech Ltd | A process for the preparation of chymosin |
| EP0122080B1 (de) * | 1983-03-25 | 1989-09-13 | Celltech Limited | Verfahren zur Herstellung eines Proteins |
| US5340926A (en) * | 1983-03-25 | 1994-08-23 | Celltech, Limited | Process for the recovery of recombinantly produced protein from insoluble aggregate |
| CA1267615A (en) * | 1984-08-27 | 1990-04-10 | Dan Hadary | Method for recovering purified growth hormones from genetically engineered microorganisms |
| JPH01222780A (ja) | 1988-03-02 | 1989-09-06 | Tosoh Corp | ウロキナーゼ前駆体様蛋白質のリフォールディング方法 |
| US5064943A (en) * | 1988-12-16 | 1991-11-12 | American Cyanamid Company | Method for solubilization and naturation of somatotropin |
| EP0574050A1 (de) | 1992-05-19 | 1993-12-15 | Gist-Brocades N.V. | Trennung und Reinigung von Fermentationsprodukt in grossen Massstab |
| BE1009844A3 (fr) | 1995-12-22 | 1997-10-07 | Genencor Int | Procede de recolte d'une proteine. |
-
2000
- 2000-01-24 EP EP00901052A patent/EP1149160B1/de not_active Expired - Lifetime
- 2000-01-24 CN CNB008030650A patent/CN1181197C/zh not_active Expired - Lifetime
- 2000-01-24 WO PCT/DK2000/000029 patent/WO2000043502A1/en not_active Ceased
- 2000-01-24 JP JP2000594910A patent/JP2002534975A/ja active Pending
- 2000-01-24 US US09/489,712 patent/US6316240B1/en not_active Expired - Lifetime
- 2000-01-24 AU AU20931/00A patent/AU2093100A/en not_active Abandoned
- 2000-01-24 DK DK00901052T patent/DK1149160T3/da active
- 2000-01-24 DE DE60027406T patent/DE60027406T2/de not_active Expired - Lifetime
- 2000-01-24 AT AT00901052T patent/ATE323759T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| DK1149160T3 (da) | 2006-08-14 |
| EP1149160B1 (de) | 2006-04-19 |
| WO2000043502A1 (en) | 2000-07-27 |
| DE60027406D1 (de) | 2006-05-24 |
| CN1181197C (zh) | 2004-12-22 |
| DE60027406T2 (de) | 2006-11-23 |
| JP2002534975A (ja) | 2002-10-22 |
| US6316240B1 (en) | 2001-11-13 |
| AU2093100A (en) | 2000-08-07 |
| CN1337999A (zh) | 2002-02-27 |
| EP1149160A1 (de) | 2001-10-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |