ATE323759T1 - Rückgewinnung eines proteins bei hohem ph-wert - Google Patents

Rückgewinnung eines proteins bei hohem ph-wert

Info

Publication number
ATE323759T1
ATE323759T1 AT00901052T AT00901052T ATE323759T1 AT E323759 T1 ATE323759 T1 AT E323759T1 AT 00901052 T AT00901052 T AT 00901052T AT 00901052 T AT00901052 T AT 00901052T AT E323759 T1 ATE323759 T1 AT E323759T1
Authority
AT
Austria
Prior art keywords
protein
recovery
solution
state
glycosidase
Prior art date
Application number
AT00901052T
Other languages
English (en)
Inventor
Mads Aage Laustsen
Curran Matthew Simpson
Michael O'reilly
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE323759T1 publication Critical patent/ATE323759T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
AT00901052T 1999-01-25 2000-01-24 Rückgewinnung eines proteins bei hohem ph-wert ATE323759T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA199900085 1999-01-25
DKPA199901491 1999-10-18

Publications (1)

Publication Number Publication Date
ATE323759T1 true ATE323759T1 (de) 2006-05-15

Family

ID=26063274

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00901052T ATE323759T1 (de) 1999-01-25 2000-01-24 Rückgewinnung eines proteins bei hohem ph-wert

Country Status (9)

Country Link
US (1) US6316240B1 (de)
EP (1) EP1149160B1 (de)
JP (1) JP2002534975A (de)
CN (1) CN1181197C (de)
AT (1) ATE323759T1 (de)
AU (1) AU2093100A (de)
DE (1) DE60027406T2 (de)
DK (1) DK1149160T3 (de)
WO (1) WO2000043502A1 (de)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUP0300840A2 (hu) 2000-07-28 2003-07-28 Henkel Kommanditgesellschaft Auf Aktien Új, Bacillus sp. A 7-7 (DSM 12368)-ból extrahált amilolitikus enzim, valamint használata mosó- és tisztítószerekben
RU2325819C2 (ru) * 2006-03-16 2008-06-10 Вячеслав Юрьевич Елупов Йодированные крупы
CN101631797B (zh) * 2007-03-15 2015-12-16 诺维信公司 发酵液中蛋白酶晶体的溶解
US20110189344A1 (en) * 2008-06-09 2011-08-04 Michael Bodo Recovery of insoluble enzyme from fermentation broth and formulation of insoluble enzyme
GB0908770D0 (en) 2009-04-24 2009-07-01 Danisco Method
US20150291656A1 (en) * 2012-11-01 2015-10-15 Novozymes A/S Method for removal of dna
DK3137483T3 (en) * 2014-04-30 2019-04-23 Novozymes As Method of reducing the DNA content of a fermentation broth
BR112018011567A2 (pt) 2015-12-09 2018-11-27 Basf Se método de purificação de uma proteína de interesse, e, uso de um método.
US20200109388A1 (en) 2017-04-03 2020-04-09 Novozymes A/S Recovery Process
US11732250B2 (en) 2018-04-26 2023-08-22 Basf Se Lipase enzymes
WO2019211143A1 (en) 2018-05-03 2019-11-07 Basf Se Amylase enzymes
US20220186234A1 (en) 2019-02-20 2022-06-16 Basf Se Industrial Fermentation Process for Bacillus Using Defined Medium and Trace Element Feed
WO2020169563A1 (en) 2019-02-20 2020-08-27 Basf Se Industrial fermentation process for bacillus using defined medium and magnesium feed
US20220162576A1 (en) 2019-03-25 2022-05-26 Basf Se Amylase enzymes
WO2020193532A1 (en) 2019-03-25 2020-10-01 Basf Se Cleaning composition having amylase enzymes
WO2020193534A2 (en) 2019-03-25 2020-10-01 Basf Se Amylase enzymes
EP3983425B1 (de) 2019-06-13 2025-10-01 Basf Se Verfahren zur rückgewinnung eines proteins aus fermentationsbrühe mithilfe eines divalenten kations
EP3994273A1 (de) 2019-07-02 2022-05-11 Basf Se Verfahren zur herstellung eines fermentationsmediums
WO2021004830A1 (en) 2019-07-05 2021-01-14 Basf Se Industrial fermentation process for microbial cells using a fed-batch pre-culture
BR112022000573A2 (pt) 2019-08-22 2022-03-15 Basf Se Polipeptídeo isolado, sintético ou recombinante com atividade alfa-amilase, ácido nucleico isolado, sintético ou recombinante, construto de ácido nucleico, vetor de expressão, célula hospedeira, composição, método para produzir polipeptídeo isolado, sintético ou recombinante, método para preparar uma massa ou um produto de panificação, e, métodos para usar o polipeptídeo isolado, sintético ou recombinante e um domínio c de uma primeira amilase
EP4047088A1 (de) 2021-02-22 2022-08-24 Basf Se Amylasevarianten
EP4294917A1 (de) 2021-02-22 2023-12-27 Basf Se Amylasevarianten
US20260062690A1 (en) 2022-08-02 2026-03-05 Basf Se Stabilized protein production process using bacillus host cells
WO2024033135A2 (en) 2022-08-11 2024-02-15 Basf Se Amylase variants
JP2025526654A (ja) 2022-08-11 2025-08-15 ビーエーエスエフ ソシエタス・ヨーロピア プロテアーゼ、マンナナーゼ及び／又はセルラーゼを含む酵素組成物
US20260055347A1 (en) 2022-08-11 2026-02-26 Basf Se Amylase variants
JP2025526759A (ja) 2022-08-11 2025-08-15 ビーエーエスエフ ソシエタス・ヨーロピア アミラーゼを含む酵素組成物
WO2024094733A1 (en) 2022-11-04 2024-05-10 Basf Se Polypeptides having protease activity for use in detergent compositions
CN120693402A (zh) 2022-11-04 2025-09-23 巴斯夫欧洲公司 用于在洗涤剂组合物中使用的具有蛋白酶活性的多肽
CN120153068A (zh) 2022-11-04 2025-06-13 巴斯夫欧洲公司 用于在洗涤剂组合物中使用的具有蛋白酶活性的多肽
EP4389864A1 (de) 2022-12-20 2024-06-26 Basf Se Cutinasen
WO2025031865A1 (en) 2023-08-08 2025-02-13 Basf Se Improved bacillus cell with inactivated metalloprotease
WO2025202374A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
EP4624572A1 (de) 2024-03-27 2025-10-01 Basf Se Polypeptide mit proteaseaktivität zur verwendung in waschmittelzusammensetzungen
WO2025202370A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202379A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202369A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202372A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983004418A1 (en) * 1982-06-07 1983-12-22 Celltech Limited A process for the preparation of chymosin
ATE107356T1 (de) 1983-03-25 1994-07-15 Celltech Ltd Verfahren zur herstellung eines proteins.
US5340926A (en) * 1983-03-25 1994-08-23 Celltech, Limited Process for the recovery of recombinantly produced protein from insoluble aggregate
CA1267615A (en) * 1984-08-27 1990-04-10 Dan Hadary Method for recovering purified growth hormones from genetically engineered microorganisms
JPH01222780A (ja) 1988-03-02 1989-09-06 Tosoh Corp ウロキナーゼ前駆体様蛋白質のリフォールディング方法
US5064943A (en) * 1988-12-16 1991-11-12 American Cyanamid Company Method for solubilization and naturation of somatotropin
EP0574050A1 (de) 1992-05-19 1993-12-15 Gist-Brocades N.V. Trennung und Reinigung von Fermentationsprodukt in grossen Massstab
BE1009844A3 (fr) 1995-12-22 1997-10-07 Genencor Int Procede de recolte d'une proteine.

Also Published As

Publication number Publication date
EP1149160B1 (de) 2006-04-19
DK1149160T3 (da) 2006-08-14
US6316240B1 (en) 2001-11-13
CN1337999A (zh) 2002-02-27
CN1181197C (zh) 2004-12-22
JP2002534975A (ja) 2002-10-22
DE60027406D1 (de) 2006-05-24
AU2093100A (en) 2000-08-07
EP1149160A1 (de) 2001-10-31
WO2000043502A1 (en) 2000-07-27
DE60027406T2 (de) 2006-11-23

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