ATE327341T1 - Verfahren zur herstellung von polypeptiden in einem zellfreien system und vorrichtung zu deren ausführung - Google Patents

Verfahren zur herstellung von polypeptiden in einem zellfreien system und vorrichtung zu deren ausführung

Info

Publication number
ATE327341T1
ATE327341T1 AT99917898T AT99917898T ATE327341T1 AT E327341 T1 ATE327341 T1 AT E327341T1 AT 99917898 T AT99917898 T AT 99917898T AT 99917898 T AT99917898 T AT 99917898T AT E327341 T1 ATE327341 T1 AT E327341T1
Authority
AT
Austria
Prior art keywords
fraction
cell
free system
molecular weight
producing polypeptides
Prior art date
Application number
AT99917898T
Other languages
English (en)
Inventor
Sergey Vladimirovich Biryukov
Peter Nikolayevich Simonenko
Vladimir Anatolievich Shirokov
Sergey Gennadievich Majorov
Alexander Sergeyevich Spirin
Original Assignee
Roche Diagnostics Gmbh
Inst Of Protein Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh, Inst Of Protein Res filed Critical Roche Diagnostics Gmbh
Application granted granted Critical
Publication of ATE327341T1 publication Critical patent/ATE327341T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/16—Hollow fibers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
AT99917898T 1998-03-31 1999-03-27 Verfahren zur herstellung von polypeptiden in einem zellfreien system und vorrichtung zu deren ausführung ATE327341T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU98105294/13A RU2148649C1 (ru) 1998-03-31 1998-03-31 Способ получения полипептидов в бесклеточной системе (варианты) и устройство для его осуществления

Publications (1)

Publication Number Publication Date
ATE327341T1 true ATE327341T1 (de) 2006-06-15

Family

ID=20203746

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99917898T ATE327341T1 (de) 1998-03-31 1999-03-27 Verfahren zur herstellung von polypeptiden in einem zellfreien system und vorrichtung zu deren ausführung

Country Status (8)

Country Link
US (2) US6518058B1 (de)
EP (1) EP1068348B1 (de)
JP (1) JP3813817B2 (de)
AT (1) ATE327341T1 (de)
DE (1) DE69931479T2 (de)
ES (1) ES2265685T3 (de)
RU (1) RU2148649C1 (de)
WO (1) WO1999050436A1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2169154C2 (ru) * 1999-03-25 2001-06-20 Институт белка РАН Способ получения полипептидов в бесклеточной системе
US20040115760A1 (en) 1999-06-18 2004-06-17 Thomas Metzler Method and device for carrying out biochemical reactions with a high throughput
ES2229587T3 (es) * 1999-06-18 2005-04-16 Roche Diagnostics Gmbh Procedimiento y dispositivo para llevar a cabo reacciones bioquimicas con alto rendimiento.
DE10137792A1 (de) * 2001-08-06 2003-02-27 Erdmann Volker Verfahren zur Genexpression
EP1295894B1 (de) 2001-09-25 2011-09-14 F. Hoffmann-La Roche AG In vitro Verfahren zur sequenzspezifischen Biotinylierung von Polypeptiden
JP2004042012A (ja) * 2001-10-26 2004-02-12 Nec Corp 分離装置、分析システム、分離方法および分離装置の製造方法
RU2239490C2 (ru) * 2002-12-25 2004-11-10 Закрытое акционерное общество "Плазмофильтр" Мембранно-сорбционный элемент и способ его изготовления
JP2005007352A (ja) * 2003-06-20 2005-01-13 Sharp Corp 粒子の分離方法及び分離装置並びに検出装置
DK1730313T3 (da) * 2004-03-25 2013-01-07 Univ Leland Stanford Junior Forøget proteinekspressionsudbytte i cellefri proteinsyntesesystemer ved tilsætning af skumdæmpende midler
US20070224662A1 (en) * 2004-12-17 2007-09-27 Jun Luo Post-translational modification of proteins in cell-free expression systems
US20080187963A1 (en) * 2005-07-04 2008-08-07 Max-Planck Gesellschaft Zur Foerderung Der Wissenschaften E.V. Use of Lepa for Improving the Accuracy of Protein Synthesis in Vitro
US20070117179A1 (en) * 2005-09-27 2007-05-24 Invitrogen Corporation In vitro protein synthesis systems for membrane proteins that include adolipoproteins and phospholipid-adolipoprotein particles
WO2008002668A2 (en) * 2006-06-29 2008-01-03 Searete Llc Methods for arbitrary peptide synthesis
US7993873B2 (en) 2006-06-29 2011-08-09 The Invention Science Fund I, Llc Apparatus for arbitrary peptide synthesis
EP2118129A4 (de) * 2007-03-01 2010-04-28 Life Technologies Corp Isolierte phospholipidproteinpartikel
ES2570460T3 (es) 2009-02-20 2016-05-18 Hoffmann La Roche Método de obtención de ácido nucleico que codifica inmunoglobulina
DE102010048422B4 (de) * 2010-10-15 2016-01-07 Thomas Melin Verfahren zur Produktisolierung und Substratzufuhr in einem Bioreaktor
BR112014004566A2 (pt) 2011-09-21 2017-06-13 Hoffmann La Roche método de amplificação e quantificação de cadeias leve e pesada de igg cognato, método de obtenção de anticorpo monoclonal, métodos de produção de fragmento de fab de imunoglobulina e de imunoglobulina, ácido nucleico e kit
WO2014144583A2 (en) 2013-03-15 2014-09-18 Northwestern University Methods for cell- free protein synthesis
US11530432B2 (en) 2018-03-19 2022-12-20 Northwestern University Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
WO2019204346A1 (en) 2018-04-16 2019-10-24 Northwestern University METHODS FOR CO-ACTIVATING IN VITRO NON-STANDARD AMINO ACID (nsAA) INCORPORATION AND GLYCOSYLATION IN CRUDE CELLLYSATES
WO2020040840A2 (en) 2018-06-01 2020-02-27 Northwestern University Expanding the chemical substrates for genetic code reprogramming
US12421537B2 (en) 2018-07-06 2025-09-23 Northwestern University Ribosome variants for sequence defined polymer synthesis
US12098433B2 (en) 2018-08-03 2024-09-24 Northwestern University On demand, portable, cell-free molecular sensing platform
US11987830B2 (en) * 2019-01-24 2024-05-21 Lawrence Livermore National Security, Llc Cell-free protein synthesis systems
US12325884B2 (en) 2019-03-04 2025-06-10 Northwestern University Riboswitch-based fluoride sensing in cell-free extract
RU199182U1 (ru) * 2019-12-10 2020-08-19 Федеральное государственное бюджетное учреждение науки Институт белка Российской академии наук (ИБ РАН) Устройство для синтеза белков в бесклеточной системе
CN113512088B (zh) * 2021-05-08 2022-09-27 苏州珀罗汀生物技术有限公司 一种蛋白连续生产装置及方法
JP2024541606A (ja) * 2021-11-30 2024-11-08 康碼（上海）生物科技有限公司 インビトロ生合成の反応装置及びインビトロ生合成方法
EP4606905A1 (de) * 2024-02-21 2025-08-27 Invitris GmbH Kontinuierliche proteinexpression

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2726313C3 (de) * 1977-06-10 1980-02-07 Battelle-Institut E.V., 6000 Frankfurt Verfahren zur in-vitro-Biosynthese von Hormonen, insbesondere von Insulin
US4341631A (en) * 1979-09-07 1982-07-27 Union Carbide Corporation Ultrafiltration and reverse osmosis device comprising plural carbon tubes bonded together
US4786597A (en) 1981-04-15 1988-11-22 University Patents, Inc. Method and apparatus for conducting catalytic reactions with simultaneous product separation and recovery
US5135853A (en) 1983-07-22 1992-08-04 Rensselaer Polytechnic Institute Three compartment bioreactor and method of use
US4956289A (en) * 1987-03-16 1990-09-11 Brunswick Corporation Thin film membrane enzyme reactor and method of using same
US5605835A (en) * 1988-05-23 1997-02-25 Regents Of The University Of Minnesota Bioreactor device with application as a bioartificial liver
US5478730A (en) * 1988-12-21 1995-12-26 Institute Of Protein Research Method of preparing polypeptides in cell-free translation system
ATE147787T1 (de) * 1989-07-31 1997-02-15 Inst Of Protein Research Russi Verfahren zur herstellung von polypeptiden in einem zellfreien system
US5656421A (en) * 1990-02-15 1997-08-12 Unisyn Technologies, Inc. Multi-bioreactor hollow fiber cell propagation system and method
US5198110A (en) * 1990-07-02 1993-03-30 Asahi Medical Co., Ltd. Bundle of permselective hollow fibers and a fluid separator containing the same
US5366625A (en) * 1992-03-04 1994-11-22 Pedersen Steven K Cartridge of hybrid unitary wafers of hollow fiber membranes and module containing a stack of post-potted cartridges
US5434079A (en) * 1993-02-12 1995-07-18 The United States Of America As Represented By The Department Of Health And Human Services Apparatus and process for continuous in vitro synthesis of proteins
JPH0768136A (ja) * 1993-09-02 1995-03-14 Tsuchiya Mfg Co Ltd 中空糸膜型分離モジュ−ル及びその製造方法
DE4331596A1 (de) * 1993-09-17 1995-03-23 Boehringer Mannheim Gmbh Verfahren zur quantitativen Analyse von Probenflüssigkeiten
KR0131166B1 (ko) * 1994-05-04 1998-04-11 최차용 무세포시스템에서 단백질을 제조하는 방법

Also Published As

Publication number Publication date
US6518058B1 (en) 2003-02-11
WO1999050436A1 (en) 1999-10-07
ES2265685T3 (es) 2007-02-16
EP1068348B1 (de) 2006-05-24
DE69931479D1 (de) 2006-06-29
DE69931479T2 (de) 2007-02-22
JP3813817B2 (ja) 2006-08-23
US20040086959A1 (en) 2004-05-06
RU2148649C1 (ru) 2000-05-10
EP1068348A1 (de) 2001-01-17
JP2002509730A (ja) 2002-04-02

Similar Documents

Publication Publication Date Title
ATE327341T1 (de) Verfahren zur herstellung von polypeptiden in einem zellfreien system und vorrichtung zu deren ausführung
DE69423631D1 (de) Thermit-zusammensetzungen zur verwendung als gaserzeugende körper
EP0710195A4 (de) Thermit-zusammensetzungen zur verwendung als gaserzeugende körper
DE3854757D1 (de) Verfahren zur elektrokatalytischen Umsetzung von leichten Kohlenwasserstoffen in Synthesegas.
ATE217962T1 (de) Vorrichtung zur parallel-ausrichtung von makromolekülen und verwendung davon
DE68911280D1 (de) Flüssigphaseverfahren zur Herstellung von Methanol aus Synthesegas.
MX9603532A (es) Sistema de suministro de acido nucleico, metodo de sintesis y sus usos.
DE69818445D1 (de) Verwendung von verbindungen die an ein zytoplasmatischen dipeptidase binden zur potenzierung des immunantworts
DE69615988D1 (de) Vorrichtung zur elektrolytischen Herstellung von Sauerstoff-Wasserstoffgas
ATE266671T1 (de) Nukleinsäuresynthese unter verwendung von mittels licht abspaltbaren schutzgruppen
DE59800654D1 (de) Verfahren und vorrichtung zur erzeugung von brenn-, synthese- und reduktionsgas aus festen brennstoffen
DE69615522D1 (de) Verfahren zur Entfernung von Wasser, Saüren und Benzin aus Erdgas, unter Verwendung eines Lösungsmittelgemisches
ATE230763T1 (de) Verfahren zur herstellung von uronsäureblöcken aus alginsäure
DE69402624D1 (de) Vorrichtung zur Lieferung von Hochdruckgas
DE69418008D1 (de) Gaserzeugende Zusammensetzung zur Verwendung in Aluminiumbauteilen
EP1424387A4 (de) Expansionskulturverfahren für antigenspezifische zytotoxische t-lymphozyten
ATE437887T1 (de) Vorrichtung zur herstellung von oligomeren, insbesondere peptoiden, mit rückführung der reagenzien
ATE317912T1 (de) Verfahren zur herstellung von beta-lactamen
DE69912839D1 (de) Nrihydroxstilbene enthaltende produkte und deren derivate und verfahren zur deren herstellung und ihre verwendung
DE68905379D1 (de) Verfahren zur reinigung von stickstofftrifluoridgas.
ATA901987A (de) Vorrichtung zur erzeugung von gas aus festen brennstoffen
DE69722678D1 (de) Vorrichtung und verfahren um ein strahlrohr zu verschieben
DE69605607D1 (de) Verfahren zur Umsetzung von Synthesegas in flüssiger Phase
DE69823371D1 (de) Vorrichtung und verfahren zur herstellung von flüssigen und gegebenfalls von gasförmigen produkten aus gasförmigen reaktanten
ATE156118T1 (de) Verfahren zur herstellung von fibrinogen-rezeptor-antagonisten

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties