ATE329060T1 - Mrna amplifizierung - Google Patents

Mrna amplifizierung

Info

Publication number
ATE329060T1
ATE329060T1 AT01915408T AT01915408T ATE329060T1 AT E329060 T1 ATE329060 T1 AT E329060T1 AT 01915408 T AT01915408 T AT 01915408T AT 01915408 T AT01915408 T AT 01915408T AT E329060 T1 ATE329060 T1 AT E329060T1
Authority
AT
Austria
Prior art keywords
poly
primer
flank
tail
cdna
Prior art date
Application number
AT01915408T
Other languages
English (en)
Inventor
Dietlind Zohlnhoefer
Christoph Klein
Original Assignee
Micromet Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Micromet Ag filed Critical Micromet Ag
Application granted granted Critical
Publication of ATE329060T1 publication Critical patent/ATE329060T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT01915408T 2000-03-24 2001-03-23 Mrna amplifizierung ATE329060T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP00106450 2000-03-24

Publications (1)

Publication Number Publication Date
ATE329060T1 true ATE329060T1 (de) 2006-06-15

Family

ID=8168230

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01915408T ATE329060T1 (de) 2000-03-24 2001-03-23 Mrna amplifizierung

Country Status (11)

Country Link
US (1) US7229760B2 (de)
EP (2) EP1370683B1 (de)
JP (1) JP2004508010A (de)
AT (1) ATE329060T1 (de)
AU (2) AU4250901A (de)
CA (1) CA2402534C (de)
DE (1) DE60120501T2 (de)
DK (1) DK1370683T3 (de)
ES (1) ES2264440T3 (de)
IL (1) IL151872A0 (de)
WO (1) WO2001071027A2 (de)

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Also Published As

Publication number Publication date
IL151872A0 (en) 2003-04-10
DK1370683T3 (da) 2006-10-02
AU2001242509B2 (en) 2007-08-23
DE60120501T2 (de) 2007-01-11
WO2001071027A2 (en) 2001-09-27
AU4250901A (en) 2001-10-03
US20040029124A1 (en) 2004-02-12
EP1370683A2 (de) 2003-12-17
ES2264440T3 (es) 2007-01-01
WO2001071027A3 (en) 2003-10-09
EP1370683B1 (de) 2006-06-07
CA2402534A1 (en) 2001-09-27
JP2004508010A (ja) 2004-03-18
CA2402534C (en) 2011-05-24
DE60120501D1 (de) 2006-07-20
EP1728874B1 (de) 2012-09-26
US7229760B2 (en) 2007-06-12
EP1728874A1 (de) 2006-12-06

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