ATE329060T1 - Mrna amplifizierung - Google Patents

Mrna amplifizierung

Info

Publication number
ATE329060T1
ATE329060T1 AT01915408T AT01915408T ATE329060T1 AT E329060 T1 ATE329060 T1 AT E329060T1 AT 01915408 T AT01915408 T AT 01915408T AT 01915408 T AT01915408 T AT 01915408T AT E329060 T1 ATE329060 T1 AT E329060T1
Authority
AT
Austria
Prior art keywords
poly
primer
flank
tail
cdna
Prior art date
Application number
AT01915408T
Other languages
English (en)
Inventor
Dietlind Zohlnhoefer
Christoph Klein
Original Assignee
Micromet Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Micromet Ag filed Critical Micromet Ag
Application granted granted Critical
Publication of ATE329060T1 publication Critical patent/ATE329060T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT01915408T 2000-03-24 2001-03-23 Mrna amplifizierung ATE329060T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP00106450 2000-03-24

Publications (1)

Publication Number Publication Date
ATE329060T1 true ATE329060T1 (de) 2006-06-15

Family

ID=8168230

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01915408T ATE329060T1 (de) 2000-03-24 2001-03-23 Mrna amplifizierung

Country Status (11)

Country Link
US (1) US7229760B2 (de)
EP (2) EP1728874B1 (de)
JP (1) JP2004508010A (de)
AT (1) ATE329060T1 (de)
AU (2) AU2001242509B2 (de)
CA (1) CA2402534C (de)
DE (1) DE60120501T2 (de)
DK (1) DK1370683T3 (de)
ES (1) ES2264440T3 (de)
IL (1) IL151872A0 (de)
WO (1) WO2001071027A2 (de)

Families Citing this family (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE488529T1 (de) * 1999-07-28 2010-12-15 Genentech Inc Zusammensetzungen und verfahren zur behandlung von tumoren
US7745180B2 (en) * 2002-04-24 2010-06-29 Hitachi Chemical Co., Ltd. Device and method for high-throughput quantification of mRNA from whole blood
US20040058330A1 (en) * 2002-09-20 2004-03-25 Prokaria, Ltd. Methods of use for thermostable RNA ligases
US20100022414A1 (en) 2008-07-18 2010-01-28 Raindance Technologies, Inc. Droplet Libraries
JP2007513399A (ja) * 2003-10-10 2007-05-24 バイオフィジカル コーポレーション 生化学画像の生成及びその使用方法
ES2564659T3 (es) * 2004-09-30 2016-03-28 Epigenomics Ag Método para proveer fragmentos de ADN derivados de una muestra archivada
EP1984738A2 (de) 2006-01-11 2008-10-29 Raindance Technologies, Inc. Mikrofluidische vorrichtungen und verfahren zur verwendung bei der bildung und kontrolle von nanoreaktoren
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP4190448A3 (de) 2006-05-11 2023-09-20 Bio-Rad Laboratories, Inc. Mikrofluidische vorrichtungen
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
EP4435111A1 (de) 2010-02-12 2024-09-25 Bio-Rad Laboratories, Inc. Digitale analytanalyse
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US20110224088A1 (en) * 2010-03-11 2011-09-15 Heidi Lyng Biomarkers for subtypes of cervical cancer
WO2011156734A2 (en) * 2010-06-11 2011-12-15 Hitachi Chemical Co., Ltd. Method of characterizing vascular diseases
JP5755326B2 (ja) 2010-06-11 2015-07-29 日立化成株式会社 腎機能の特徴を決定する方法
EP2675819B1 (de) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Zusammensetzungen und verfahren für molekulare etikettierung
WO2012167142A2 (en) 2011-06-02 2012-12-06 Raindance Technolgies, Inc. Enzyme quantification
EP2717989B1 (de) 2011-06-10 2018-05-30 Hitachi Chemical Co., Ltd. Vesikelaufnahmevorrichtungen und verwendungsverfahren dafür
US9541480B2 (en) 2011-06-29 2017-01-10 Academia Sinica Capture, purification, and release of biological substances using a surface coating
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
JP6297676B2 (ja) 2013-05-06 2018-03-20 日立化成株式会社 標的分子を捕捉するためのデバイス及び方法
CA2925393C (en) 2013-10-11 2023-03-07 Dimiter Dimitrov Tem8 antibodies and their use
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
WO2015103549A1 (en) 2014-01-03 2015-07-09 The United States Of America, As Represented By The Secretary Department Of Health And Human Services Neutralizing antibodies to hiv-1 env and their use
TW201623605A (zh) 2014-04-01 2016-07-01 中央研究院 用於癌症診斷及預後之方法及系統
CN105381824B (zh) 2014-08-26 2019-04-23 中央研究院 收集器架构布局设计
US10266895B2 (en) 2014-11-05 2019-04-23 Hitachi Chemical Company Ltd. Exosomes and microvesicles in intestinal luminal fluids and stool and use of same for the assessment of inflammatory bowel disease
US10370719B2 (en) 2014-11-12 2019-08-06 Hitachi Chemical Co., Ltd. Method and device for diagnosing organ injury
EP3875481B1 (de) 2014-11-14 2025-01-22 The U.S.A. as represented by the Secretary, Department of Health and Human Services Neutralisierende antikörper gegen ebola-virus-glycoprotein und deren verwendung
KR20170140180A (ko) 2015-02-24 2017-12-20 더 유나이티드 스테이츠 오브 어메리카, 애즈 리프리젠티드 바이 더 세크러테리, 디파트먼트 오브 헬쓰 앤드 휴먼 서비씨즈 중동 호흡기 증후군 코로나 바이러스 면역원, 항체 및 그 용도
SMT202000255T1 (it) 2015-03-20 2020-07-08 The United States Of America As Represented By The Secretary Department Of Health And Human Services Anticorpi neutralizzanti diretti contro gp120 e loro utilizzo
US11028443B2 (en) 2015-08-31 2021-06-08 Showa Denko Materials Co., Ltd. Molecular methods for assessing urothelial disease
AU2016335750B2 (en) 2015-10-07 2023-05-25 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services IL-7R-alpha specific antibodies for treating acute lymphoblastic leukemia
AU2016349392B2 (en) 2015-11-03 2023-07-13 The Trustees Of Columbia University In The City Of New York Neutralizing antibodies to HIV-1 gp41 and their use
US10107726B2 (en) 2016-03-16 2018-10-23 Cellmax, Ltd. Collection of suspended cells using a transferable membrane
WO2017192589A1 (en) 2016-05-02 2017-11-09 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Neutralizing antibodies to influenza ha and their use and identification
EP3580235B1 (de) 2017-02-10 2024-05-01 The United States of America, as represented by the Secretary, Department of Health and Human Services Neutralisierende antikörper gegen das plasmodium-falciparum-circumsporozoit-protein und verwendung davon
EP3724223A1 (de) 2018-01-02 2020-10-21 The United States of America, as represented by The Secretary, Department of Health and Human Services Neutralisierender antikörper gegen ebola-virus-glycoprotein und deren verwendung
WO2019165122A1 (en) 2018-02-21 2019-08-29 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Neutralizing antibodies to hiv-1 env and their use
SG11202105276TA (en) 2018-12-03 2021-06-29 Univ Texas Oligo-benzamide analogs and their use in cancer treatment
EP3883609A2 (de) 2018-12-20 2021-09-29 The United States of America, as represented by the Secretary, Department of Health and Human Services Glykoprotein-spezifische monoklonale antikörper des ebola-virus und deren verwendungen
WO2020227228A2 (en) 2019-05-03 2020-11-12 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Neutralizing antibodies to plasmodium falciparum circumsporozoite protein and their use
US12559544B2 (en) 2019-05-21 2026-02-24 University Of Georgia Research Foundation, Inc. Antibodies that bind human metapneumovirus fusion protein and their use
AU2020299382A1 (en) 2019-07-02 2022-01-20 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Monoclonal antibodies that bind EGFRvIII and their use
CN114324881B (zh) * 2020-10-10 2025-10-31 深圳华大生命科学研究院 一种高通量检测若干个蛋白之间相互作用的方法及其专用试剂盒
WO2022132904A1 (en) 2020-12-17 2022-06-23 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Human monoclonal antibodies targeting sars-cov-2
CN117642178A (zh) 2021-02-09 2024-03-01 美国政府(由卫生和人类服务部的部长所代表) 靶向冠状病毒刺突蛋白的抗体
CN117396502A (zh) 2021-02-09 2024-01-12 佐治亚大学研究基金会有限公司 针对肺炎球菌抗原的人类单克隆抗体
AU2022345251A1 (en) 2021-09-17 2024-03-28 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Synthetic humanized llama nanobody library and use thereof to identify sars-cov-2 neutralizing antibodies
EP4476251A1 (de) 2022-02-10 2024-12-18 The United States of America, as represented by the Secretary, Department of Health and Human Services Humane monoklonale antikörper mit breiter targeting von coronaviren
WO2023192881A1 (en) 2022-03-28 2023-10-05 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Neutralizing antibodies to hiv-1 env and their use
EP4565329A1 (de) 2022-08-01 2025-06-11 The United States of America, as represented by the Secretary, Department of Health and Human Services An die unterseite der viralen influenza-neuraminidase bindende monoklonale antikörper
WO2024054822A1 (en) 2022-09-07 2024-03-14 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Engineered sars-cov-2 antibodies with increased neutralization breadth
WO2024064826A1 (en) 2022-09-22 2024-03-28 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Neutralizing antibodies to plasmodium falciparum circumsporozoite protein and their use
EP4638491A1 (de) 2022-12-19 2025-10-29 The United States of America, as represented by The Secretary, Department of Health and Human Services Monoklonale antikörper zur behandlung von sars-cov-2-infektionen
WO2024138151A1 (en) 2022-12-22 2024-06-27 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Ebolavirus (sudan and zaire) antibodies from non-human primates and human vaccinees
WO2024243355A1 (en) 2023-05-24 2024-11-28 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Human monoclonal antibodies that target the rh5 complex of blood-stage plasmodium falciparum
WO2025014896A1 (en) 2023-07-07 2025-01-16 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Humanized 40h3 antibody
AU2024300009A1 (en) 2023-07-21 2026-01-08 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Bispecific antibodies that broadly target coronaviruses
WO2025106427A1 (en) 2023-11-14 2025-05-22 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Neutralizing and protective monoclonal antibodies against respiratory syncytial virus (rsv)
WO2025117384A1 (en) 2023-12-01 2025-06-05 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Broadly neutralizing influenza hemagglutinin stem-directed antibodies
WO2025137284A2 (en) 2023-12-21 2025-06-26 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Broadly neutralizing antibodies against sars-cov-2 and sars-cov variants
WO2026030473A1 (en) 2024-07-31 2026-02-05 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services West nile virus neutralizing monoclonal antibodies
CN120260678A (zh) * 2025-03-07 2025-07-04 上海睿璟生物科技有限公司 一种用于rna含量检测的扩增引物、探针的设计方法、装置及试剂盒

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU632760B2 (en) 1988-07-26 1993-01-14 Genelabs Technologies, Inc. Rna and dna amplification techniques
WO1990006044A2 (en) * 1988-11-21 1990-06-14 Dynal As PROCESS FOR PRODUCING cDNA
WO1997008185A1 (en) * 1995-08-28 1997-03-06 Alphagene, Inc. Use of a 5' positional primer to produce double-stranded dna from a dna template
WO1997013877A1 (en) * 1995-10-12 1997-04-17 Lynx Therapeutics, Inc. Measurement of gene expression profiles in toxicity determination
JP2002515738A (ja) 1996-01-23 2002-05-28 アフィメトリックス,インコーポレイティド 核酸分析法
US5955269A (en) * 1996-06-20 1999-09-21 Rutgers, The State University Of New Jersey Methods of screening foods for nutraceuticals
US5955275A (en) * 1997-02-14 1999-09-21 Arcaris, Inc. Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes
US6132997A (en) * 1999-05-28 2000-10-17 Agilent Technologies Method for linear mRNA amplification

Also Published As

Publication number Publication date
WO2001071027A2 (en) 2001-09-27
CA2402534A1 (en) 2001-09-27
WO2001071027A3 (en) 2003-10-09
CA2402534C (en) 2011-05-24
AU4250901A (en) 2001-10-03
EP1370683B1 (de) 2006-06-07
EP1370683A2 (de) 2003-12-17
US7229760B2 (en) 2007-06-12
DE60120501D1 (de) 2006-07-20
ES2264440T3 (es) 2007-01-01
DE60120501T2 (de) 2007-01-11
IL151872A0 (en) 2003-04-10
US20040029124A1 (en) 2004-02-12
AU2001242509B2 (en) 2007-08-23
EP1728874A1 (de) 2006-12-06
DK1370683T3 (da) 2006-10-02
EP1728874B1 (de) 2012-09-26
JP2004508010A (ja) 2004-03-18

Similar Documents

Publication Publication Date Title
DK1370683T3 (da) mRNA-amplifikation
EP0787807A3 (de) Primers und Sondern zum Nachweis von Hepatitis C
EP1594980A4 (de) Nukleinsäureamplifikation auf basis von kügelchenemulsion
EP1845160A4 (de) Nukleotidsequenz-amplifikationsverfahren
ATE480644T1 (de) Verfahren zur quantifizierung kleiner rna- moleküle
WO2006042303A3 (en) Methods and compositions for analyzing ribonucleic acids
WO2002052031A3 (en) Nucleic acid amplification
ATE476523T1 (de) Verfahren zur genomamplifikation
WO2007066831A3 (en) Method of detecting human papilloma virus by using nucleic acid amplification method and nucleic acid chain-immobilized carrier
DE69427876D1 (de) Methode zur detektion einer gezielten nukleinsäure
EP1975249A3 (de) Nukleotid-Primer-Set und Nukleotidsonde zur Detektion des N-Acetyltransferase 2 (NAT2)-Genotyps
FR2878254B1 (fr) Composition pour l'amplification d'acides nucleiques
WO2004050911A3 (en) Method for lowering both sequence variations and increase of base lines effects in diagnostic hybridisation assay, assay for performing such a method and probe for use in the assay
WO2007021250A3 (en) Method and/or apparatus of oligonucleotide design and/or nucleic acid detection
ATE446385T1 (de) Polymorphismen im nod2/card15 gen
EP1741793A4 (de) Hybridisierungsverfahren
WO2005025408A3 (en) Assay for sars coronavirus by amplification and detection of nucleocapsid rna sequence
MXPA02000025A (es) Secuencias y metodos para la deteccion del vih-1.
WO2001023611A3 (en) Methods for detecting amplification by change of fluorescence

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1370683

Country of ref document: EP