ATE335826T1 - Proteinherstellung - Google Patents

Proteinherstellung

Info

Publication number
ATE335826T1
ATE335826T1 AT00958389T AT00958389T ATE335826T1 AT E335826 T1 ATE335826 T1 AT E335826T1 AT 00958389 T AT00958389 T AT 00958389T AT 00958389 T AT00958389 T AT 00958389T AT E335826 T1 ATE335826 T1 AT E335826T1
Authority
AT
Austria
Prior art keywords
sup
heterologous polypeptide
pro
host cell
fusion protein
Prior art date
Application number
AT00958389T
Other languages
English (en)
Inventor
Guenter Stempfer
Joerg Windisch
Franz Knauseder
Original Assignee
Sandoz Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sandoz Ag filed Critical Sandoz Ag
Application granted granted Critical
Publication of ATE335826T1 publication Critical patent/ATE335826T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
AT00958389T 1999-08-09 2000-08-07 Proteinherstellung ATE335826T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AT136799 1999-08-09

Publications (1)

Publication Number Publication Date
ATE335826T1 true ATE335826T1 (de) 2006-09-15

Family

ID=3512357

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00958389T ATE335826T1 (de) 1999-08-09 2000-08-07 Proteinherstellung

Country Status (21)

Country Link
US (2) US6936455B1 (de)
EP (1) EP1200604B1 (de)
JP (1) JP4820512B2 (de)
KR (1) KR20020026366A (de)
CN (1) CN1230543C (de)
AT (1) ATE335826T1 (de)
AU (1) AU775662B2 (de)
CA (1) CA2379571C (de)
CZ (1) CZ302366B6 (de)
DE (1) DE60029968T2 (de)
ES (1) ES2269173T3 (de)
HK (1) HK1047128B (de)
HU (1) HU230231B1 (de)
IL (1) IL147456A0 (de)
MX (1) MXPA02001424A (de)
NO (1) NO328339B1 (de)
NZ (1) NZ516869A (de)
PL (1) PL200225B1 (de)
SK (1) SK287488B6 (de)
TR (1) TR200200253T2 (de)
WO (1) WO2001011057A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2380302C (en) * 1999-08-09 2013-02-26 Biochemie Gesellschaft M.B.H. Production of proteins by autoproteolytic cleavage
DE602005019992D1 (de) 2004-03-30 2010-04-29 Relypsa Inc Kaliumbindende polymere und ihre verwendungen
AU2011253661B2 (en) * 2005-04-26 2013-06-13 Boehringer Ingelheim Rcv Gmbh & Co Kg Production of recombinant proteins by autoproteolytic cleavage of a fusion protein
EP1874825B1 (de) * 2005-04-26 2010-03-24 Sandoz AG Affinitätsliganden
GB0508435D0 (en) * 2005-04-26 2005-06-01 Sandoz Ag Organic compounds
GB0508434D0 (en) * 2005-04-26 2005-06-01 Sandoz Ag Organic compounds
WO2012029529A1 (ja) * 2010-08-31 2012-03-08 天野エンザイム株式会社 酵素を用いた卵殻膜の可溶化方法
EP2684951A1 (de) 2012-07-13 2014-01-15 Sandoz Ag Verfahren zur Herstellung eines rekombinanten Proteins von Interesse
EP2746390A1 (de) * 2012-12-19 2014-06-25 Sandoz Ag Verfahren zur Herstellung eines rekombinanten Proteins von Interesse
EP2746391A1 (de) 2012-12-19 2014-06-25 Sandoz Ag Verfahren zur Herstellung eines rekombinanten Proteins von Interesse
UY35874A (es) 2013-12-12 2015-07-31 Novartis Ag Un proceso para la preparación de una composición de proteínas pegiladas
RU2619217C1 (ru) * 2015-12-04 2017-05-12 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГБУ "ГосНИИгенетика") Температурочувствительный мутантный интеин для нерастворимой экспрессии предшественника целевого белка
EP3904525A1 (de) * 2020-04-27 2021-11-03 Kutzner, Christoph Fusionspolypeptide für die zielpeptid-produktion
CN114539425B (zh) * 2022-02-25 2023-04-28 湖南中晟全肽生化有限公司 一种提高线性多肽生物表达的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4743679A (en) * 1986-02-24 1988-05-10 Creative Biomolecules, Inc. Process for producing human epidermal growth factor and analogs thereof
DE3885431D1 (de) 1987-12-23 1993-12-09 Boehringer Ingelheim Int Expression der viral kodierten Protease P2A des HRV2.
MX219629B (es) * 1997-04-25 2004-03-30 Sembiosys Genetics Inc Metodo para separar proteinas de fusion
IL134653A0 (en) 1997-08-22 2001-04-30 Roche Diagnostics Gmbh Zymogenic protease precursors that can be autocatalytically activated and their use
WO1999010483A2 (de) 1997-08-22 1999-03-04 Roche Diagnostics Gmbh Autokatalytisch aktivierbare vorstufen von proteasen und deren verwendung

Also Published As

Publication number Publication date
US6936455B1 (en) 2005-08-30
ES2269173T3 (es) 2007-04-01
CA2379571A1 (en) 2001-02-15
NO328339B1 (no) 2010-02-01
PL200225B1 (pl) 2008-12-31
HU230231B1 (hu) 2015-10-28
HUP0202519A3 (en) 2004-07-28
NZ516869A (en) 2004-01-30
NO20020633D0 (no) 2002-02-08
EP1200604B1 (de) 2006-08-09
CN1230543C (zh) 2005-12-07
CZ2002481A3 (cs) 2002-05-15
JP4820512B2 (ja) 2011-11-24
TR200200253T2 (tr) 2002-05-21
SK287488B6 (sk) 2010-11-08
HUP0202519A2 (en) 2002-10-28
SK1852002A3 (en) 2002-07-02
DE60029968D1 (de) 2006-09-21
JP2003506093A (ja) 2003-02-18
PL353101A1 (en) 2003-10-20
US20050186564A1 (en) 2005-08-25
HK1047128B (en) 2007-06-08
IL147456A0 (en) 2002-08-14
KR20020026366A (ko) 2002-04-09
NO20020633L (no) 2002-04-05
AU775662B2 (en) 2004-08-12
CA2379571C (en) 2011-03-29
CN1369014A (zh) 2002-09-11
AU6993300A (en) 2001-03-05
WO2001011057A1 (en) 2001-02-15
HK1047128A1 (en) 2003-02-07
DE60029968T2 (de) 2006-12-21
EP1200604A1 (de) 2002-05-02
MXPA02001424A (es) 2002-08-12
CZ302366B6 (cs) 2011-04-13

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