ATE354653T1 - Verfahren zur ortspezifischen massenmutagenese - Google Patents

Verfahren zur ortspezifischen massenmutagenese

Info

Publication number
ATE354653T1
ATE354653T1 AT01965350T AT01965350T ATE354653T1 AT E354653 T1 ATE354653 T1 AT E354653T1 AT 01965350 T AT01965350 T AT 01965350T AT 01965350 T AT01965350 T AT 01965350T AT E354653 T1 ATE354653 T1 AT E354653T1
Authority
AT
Austria
Prior art keywords
mutagenesis
site
specific mass
concerns
directed
Prior art date
Application number
AT01965350T
Other languages
English (en)
Inventor
Marc Delcourt
Stephane Blesa
Original Assignee
Biomethodes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomethodes filed Critical Biomethodes
Application granted granted Critical
Publication of ATE354653T1 publication Critical patent/ATE354653T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Gyroscopes (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
AT01965350T 2000-08-25 2001-08-24 Verfahren zur ortspezifischen massenmutagenese ATE354653T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0010962A FR2813314B1 (fr) 2000-08-25 2000-08-25 Procede de mutagenese dirigee massive

Publications (1)

Publication Number Publication Date
ATE354653T1 true ATE354653T1 (de) 2007-03-15

Family

ID=8853747

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01965350T ATE354653T1 (de) 2000-08-25 2001-08-24 Verfahren zur ortspezifischen massenmutagenese

Country Status (11)

Country Link
US (1) US7202086B2 (de)
EP (1) EP1311670B1 (de)
JP (1) JP5068917B2 (de)
AT (1) ATE354653T1 (de)
AU (1) AU2001286009A1 (de)
CA (1) CA2420721C (de)
DE (1) DE60126786T2 (de)
DK (1) DK1311670T3 (de)
ES (1) ES2282285T3 (de)
FR (1) FR2813314B1 (de)
WO (1) WO2002016606A2 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
US20040142433A1 (en) * 2001-02-02 2004-07-22 Padgett Hal S. Polynucleotide sequence variants
US7582423B2 (en) 2001-02-02 2009-09-01 Novici Biotech Llc Population of polynucleotide sequence variants
CA2436214C (en) * 2001-02-02 2012-12-04 Large Scale Biology Corporation A method of increasing complementarity in a heteroduplex polynucleotide
US7838219B2 (en) * 2001-02-02 2010-11-23 Novici Biotech Llc Method of increasing complementarity in a heteroduplex
US20050153283A1 (en) * 2002-08-08 2005-07-14 Padgett Hal S. Method of increasing complementarity in a heteroduplex
FR2823219B1 (fr) * 2001-04-10 2003-07-04 Pasteur Institut Mutants de la desoxycytidine kinase possedant une activite enzymatique elargie
US7647184B2 (en) 2001-08-27 2010-01-12 Hanall Pharmaceuticals, Co. Ltd High throughput directed evolution by rational mutagenesis
US7078211B2 (en) * 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
DK1504098T4 (da) 2002-05-17 2011-05-23 Alligator Bioscience Ab Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion
DE60332358D1 (de) 2002-09-09 2010-06-10 Hanall Pharmaceutical Co Ltd Protease-resistente modifizierte interferon alpha polypeptide
DE602004020570D1 (de) * 2003-12-18 2009-05-28 Biomethodes Verfahren zur ortspezifischen Massenmutagenese
FR2883885B1 (fr) * 2005-04-05 2010-12-10 Biomethodes Recombinaison in vitro circulaire
FR2886943B1 (fr) 2005-06-10 2007-09-07 Biomethodes Sa Methode de selection de proteines stables dans des conditions physico-chimiques non standard
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
FR2897068B1 (fr) * 2006-02-03 2008-04-18 Biomethodes Sa Kanamycine kinase ii amelioree et utilisations de celle-ci
EP1991679A2 (de) * 2006-03-08 2008-11-19 Biomethodes Varianten des humanen interferon-gamma (ifngamma)
CA2693102C (en) * 2007-07-31 2020-03-10 Verenium Corporation Tailored multi-site combinatorial assembly
US20160185847A1 (en) 2012-12-17 2016-06-30 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Use of monoclonal antibodies for the treatment of inflammation and bacterial infections
US9637728B2 (en) 2013-07-26 2017-05-02 Biomethodes Variant Trichoderma reesei endoglucanases
EP3728620A4 (de) * 2017-12-20 2021-11-03 Basf Se Genstellensättigungsmutagenese(gssm)-verfahren

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4960707A (en) * 1987-08-17 1990-10-02 Associated Universities, Inc. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae
CA2079802C (en) * 1990-04-05 2001-09-18 Roberto Crea Walk-through mutagenesis
WO1993020096A1 (en) * 1991-05-08 1993-10-14 Stratagene Oligonucleotide libraries useful for producing primers
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6620597B1 (en) * 1998-01-09 2003-09-16 University Of Utah Research Foundation Method for in vitro amplification of circular DNA
US6368861B1 (en) * 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination

Also Published As

Publication number Publication date
JP2004507243A (ja) 2004-03-11
JP5068917B2 (ja) 2012-11-07
WO2002016606A3 (fr) 2002-06-27
DE60126786D1 (de) 2007-04-05
FR2813314A1 (fr) 2002-03-01
CA2420721A1 (fr) 2002-02-28
CA2420721C (fr) 2013-11-12
WO2002016606A2 (fr) 2002-02-28
DK1311670T3 (da) 2007-06-18
EP1311670B1 (de) 2007-02-21
DE60126786T2 (de) 2007-11-08
US7202086B2 (en) 2007-04-10
AU2001286009A1 (en) 2002-03-04
EP1311670A2 (de) 2003-05-21
FR2813314B1 (fr) 2004-05-07
US20040048268A1 (en) 2004-03-11
ES2282285T3 (es) 2007-10-16

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