DK1504098T4 - Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion - Google Patents

Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion

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Publication number
DK1504098T4
DK1504098T4 DK03722867.3T DK03722867T DK1504098T4 DK 1504098 T4 DK1504098 T4 DK 1504098T4 DK 03722867 T DK03722867 T DK 03722867T DK 1504098 T4 DK1504098 T4 DK 1504098T4
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Denmark
Prior art keywords
stranded polynucleotide
reaction
population
parent
polynucleotide molecules
Prior art date
Application number
DK03722867.3T
Other languages
English (en)
Other versions
DK1504098T3 (da
Inventor
Christina Furebring
Roland Carlsson
Carl Arne Krister Borrebaeck
Hager Ann-Christin Malmborg
Original Assignee
Alligator Bioscience Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=29551433&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK1504098(T4) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from GB0211369A external-priority patent/GB2388604B/en
Priority claimed from US10/321,195 external-priority patent/US7153655B2/en
Application filed by Alligator Bioscience Ab filed Critical Alligator Bioscience Ab
Application granted granted Critical
Publication of DK1504098T3 publication Critical patent/DK1504098T3/da
Publication of DK1504098T4 publication Critical patent/DK1504098T4/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Ecology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK03722867.3T 2002-05-17 2003-05-16 Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion DK1504098T4 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0211369A GB2388604B (en) 2002-05-17 2002-05-17 A method for in vitro molecular evolution of protein function
US10/321,195 US7153655B2 (en) 1998-06-16 2002-12-17 Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
PCT/GB2003/002102 WO2003097834A2 (en) 2002-05-17 2003-05-16 A method for in vitro molecular evolution of protein function

Publications (2)

Publication Number Publication Date
DK1504098T3 DK1504098T3 (da) 2007-06-11
DK1504098T4 true DK1504098T4 (da) 2011-05-23

Family

ID=29551433

Family Applications (2)

Application Number Title Priority Date Filing Date
DK03722867.3T DK1504098T4 (da) 2002-05-17 2003-05-16 Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion
DK200401821A DK200401821A (da) 2002-05-17 2004-11-23 Fremgangsmåde til in vitro molekylær udvikling af proteinfunktion

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK200401821A DK200401821A (da) 2002-05-17 2004-11-23 Fremgangsmåde til in vitro molekylær udvikling af proteinfunktion

Country Status (10)

Country Link
US (1) US7262012B2 (da)
EP (1) EP1504098B2 (da)
JP (1) JP4372679B2 (da)
AT (1) ATE356871T1 (da)
AU (1) AU2003230027A1 (da)
CA (1) CA2485506C (da)
DE (1) DE60312507T3 (da)
DK (2) DK1504098T4 (da)
ES (1) ES2285118T5 (da)
WO (1) WO2003097834A2 (da)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL345452A1 (en) * 1998-07-10 2001-12-17 Jari Pharmaceuticals Bv Chemotaxis-inhibiting protein of staphylococcus
EP1118663A1 (en) * 2000-01-07 2001-07-25 Universiteit Utrecht Nucleic acids encoding chemotaxis inhibitory polypeptides
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
EP1586583A3 (en) * 2004-04-16 2005-11-16 Alligator Bioscience AB (publ) Compounds that block C5a complement receptor and their use in therapy
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
GB0607798D0 (en) 2006-04-20 2006-05-31 Alligator Bioscience Ab Novel polypeptides and use thereof
GB0708376D0 (en) 2007-05-01 2007-06-06 Alligator Bioscience Ab Novel polypeptides and uses thereof
GB0905790D0 (en) 2009-04-03 2009-05-20 Alligator Bioscience Ab Novel polypeptides and use thereof
GB0905503D0 (en) * 2009-03-31 2009-05-13 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
GB0920258D0 (en) 2009-11-19 2010-01-06 Alligator Bioscience Ab New medical agents and use thereof
GB201019086D0 (en) 2010-11-11 2010-12-29 Imp Innovations Ltd Bacterial methods
WO2012122135A2 (en) 2011-03-04 2012-09-13 Evolvemol, Inc. Apparatus and process for isolating specific physical items within a set of physical items
GB201115280D0 (en) 2011-09-05 2011-10-19 Alligator Bioscience Ab Antibodies, uses and methods
GB201311475D0 (en) 2013-06-27 2013-08-14 Alligator Bioscience Ab Polypeptides
EP3180087B1 (en) 2014-08-12 2019-03-13 Alligator Bioscience AB Combination therapies with anti cd40 antibodies
PE20181367A1 (es) 2015-09-30 2018-08-27 Janssen Biotech Inc Anticuerpos agonistas que se unen especificamente a cd40 humana y metodos de uso
CN113892654A (zh) * 2021-10-21 2022-01-07 长春大学 一种以绿豆蛋白粉为原料制备免消化绿豆蛋白质的方法

Family Cites Families (151)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4888286A (en) 1984-02-06 1989-12-19 Creative Biomolecules, Inc. Production of gene and protein analogs through synthetic gene design using double stranded synthetic oligonucleotides
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US6492107B1 (en) 1986-11-20 2002-12-10 Stuart Kauffman Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
DE3587814T2 (de) 1985-03-30 1994-11-10 Marc Ballivet Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren.
US4994368A (en) 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
FI903489A7 (fi) 1988-11-11 1990-07-10 Medical Res Council Yhden osan sisältävät ligandit, näitä ligandeja sisältävät reseptorit, menetelmiä niiden valmistamiseksi sekä ligandien ja reseptorien käytt ö
EP0832980B1 (en) 1989-01-23 2002-06-19 Chiron Corporation Recombinant therapies for infection and hyperproliferative disorders
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
CA2016842A1 (en) 1989-05-16 1990-11-16 Richard A. Lerner Method for tapping the immunological repertoire
US5023171A (en) 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
GB8919607D0 (en) 1989-08-30 1989-10-11 Wellcome Found Novel entities for cancer therapy
AU6886991A (en) 1989-11-08 1991-06-13 United States of America, as represented by the Secretary, U.S. Department of Commerce, The A method of synthesizing double-stranded dna molecules
US5573907A (en) 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
CA2079802C (en) 1990-04-05 2001-09-18 Roberto Crea Walk-through mutagenesis
EP0528881A4 (en) 1990-04-24 1993-05-26 Stratagene Methods for phenotype creation from multiple gene populations
US5387505A (en) 1990-05-04 1995-02-07 Eastman Kodak Company Preparation and isolation of single-stranded biotinylated nucleic acids by heat avidin-biotin cleavage
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
US5858725A (en) 1990-10-10 1999-01-12 Glaxo Wellcome Inc. Preparation of chimaeric antibodies using the recombinant PCR strategy
DE69229222D1 (de) 1991-03-07 1999-06-24 Bradbury Auswahl spezifischer proteine durch ein biologisches verfahren
DE4112440C1 (da) 1991-04-16 1992-10-22 Diagen Institut Fuer Molekularbiologische Diagnostik Gmbh, 4000 Duesseldorf, De
US5514568A (en) 1991-04-26 1996-05-07 Eli Lilly And Company Enzymatic inverse polymerase chain reaction
US5512463A (en) 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
US5871907A (en) 1991-05-15 1999-02-16 Medical Research Council Methods for producing members of specific binding pairs
US5858657A (en) 1992-05-15 1999-01-12 Medical Research Council Methods for producing members of specific binding pairs
US5223408A (en) 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
JP3540315B2 (ja) 1991-09-23 2004-07-07 メディカル リサーチ カウンシル キメラ抗体の製造−組合せアプローチ
IL103059A0 (en) 1991-09-30 1993-02-21 Boehringer Ingelheim Int Conjugates for introducing nucleic acid into higher eucaryotic cells
US5521291A (en) 1991-09-30 1996-05-28 Boehringer Ingelheim International, Gmbh Conjugates for introducing nucleic acid into higher eucaryotic cells
US5270170A (en) 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
US5733731A (en) 1991-10-16 1998-03-31 Affymax Technologies N.V. Peptide library and screening method
US5252479A (en) 1991-11-08 1993-10-12 Research Corporation Technologies, Inc. Safe vector for gene therapy
WO1993012257A1 (en) 1991-12-12 1993-06-24 Hybritech Incorporated Enzymatic inverse polymerase chain reaction library mutagenesis
US5395750A (en) 1992-02-28 1995-03-07 Hoffmann-La Roche Inc. Methods for producing proteins which bind to predetermined antigens
WO1994012632A1 (en) 1992-11-27 1994-06-09 University College London Improvements in nucleic acid synthesis by pcr
US5733753A (en) 1992-12-22 1998-03-31 Novo Nordisk A/S Amplification of genomic DNA by site specific integration of a selectable marker construct
US5491074A (en) 1993-04-01 1996-02-13 Affymax Technologies Nv Association peptides
SE9304060D0 (sv) 1993-12-06 1993-12-06 Bioinvent Int Ab Sätt att selektera specifika bakteriofager
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
WO1996017056A1 (en) 1994-12-02 1996-06-06 Institut Pasteur Hypermutagenesis
US5869451A (en) 1995-06-07 1999-02-09 Glaxo Group Limited Peptides and compounds that bind to a receptor
CN1966520B (zh) 1995-06-07 2012-07-11 葛兰素集团有限公司 结合血小板生成素受体的肽和化合物
WO1997007205A1 (en) 1995-08-11 1997-02-27 Novo Nordisk A/S Method for preparing polypeptide variants
EP0859841B1 (en) 1995-08-18 2002-06-19 MorphoSys AG Protein/(poly)peptide libraries
US6479258B1 (en) 1995-12-07 2002-11-12 Diversa Corporation Non-stochastic generation of genetic vaccines
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US6352842B1 (en) 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
WO2000053744A2 (en) 1999-03-09 2000-09-14 Diversa Corporation End selection in directed evolution
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US6368798B1 (en) 1995-12-07 2002-04-09 Diversa Corporation Screening for novel bioactivities
US6358709B1 (en) 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
US6489145B1 (en) 1996-07-09 2002-12-03 Diversa Corporation Method of DNA shuffling
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US5851804A (en) 1996-05-06 1998-12-22 Apollon, Inc. Chimeric kanamycin resistance gene
US5877001A (en) 1996-06-17 1999-03-02 Diverso Corporation Amidase
US5763239A (en) 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
JPH1066576A (ja) 1996-08-07 1998-03-10 Novo Nordisk As 突出末端を有する２本鎖ｄｎａ及びこれを用いたｄｎａのシャフリング方法
WO1998013485A1 (en) 1996-09-27 1998-04-02 Maxygen, Inc. Methods for optimization of gene therapy by recursive sequence shuffling and selection
CA2266423A1 (en) 1996-09-27 1998-04-02 Maxygen, Inc. Methods for optimization of gene therapy by recursive sequence shuffling and selection
US5925544A (en) 1996-11-18 1999-07-20 Novo Nordisk A/S Method of homologous recombination followed by in vivo selection of DNA amplification
EP0948615B1 (en) 1996-12-20 2004-12-15 Novozymes A/S In vivo recombination
WO1998031816A1 (en) 1997-01-17 1998-07-23 Regents Of The University Of Minnesota Dna molecules and protein displaying improved triazine compound degrading ability
EP1717322B1 (en) 1997-01-17 2012-07-18 Codexis Mayflower Holdings, LLC Evolution of whole cells and organisms by recursive sequence recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
GB9701425D0 (en) 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
DK1015575T3 (da) 1997-03-18 2010-08-23 Novozymes As Shuffling af heterologe DNA-sekvenser
KR20000076363A (ko) 1997-03-18 2000-12-26 한센 핀 베네드, 안네 제헤르, 웨이콥 마리안느 Ｄｎａ 라이브러리를 제조하기 위한 시험관내 방법
DE69840382D1 (de) 1997-03-18 2009-02-05 Novozymes As Methode zur herstellung einer bibliothek mittels dna-shuffling
US6159688A (en) 1997-03-18 2000-12-12 Novo Nordisk A/S Methods of producing polynucleotide variants
US6159687A (en) 1997-03-18 2000-12-12 Novo Nordisk A/S Methods for generating recombined polynucleotides
US6291165B1 (en) 1997-03-18 2001-09-18 Novo Nordisk A/S Shuffling of heterologous DNA sequences
US6153410A (en) 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
GB9712512D0 (en) 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
AU1124499A (en) 1997-10-28 1999-05-17 Maxygen, Inc. Human papillomavirus vectors
AU1449499A (en) 1997-10-31 1999-05-24 Maxygen, Incorporated Modification of virus tropism and host range by viral genome shuffling
US7026446B1 (en) 1997-12-24 2006-04-11 Diatech Pty Ltd. Bifunctional molecules
CA2320958A1 (en) 1998-02-11 1999-08-19 Maxygen, Inc. Antigen library immunization
US20010006950A1 (en) 1998-02-11 2001-07-05 Juha Punnonen Genetic vaccine vector engineering
EP1053343A2 (en) 1998-02-11 2000-11-22 Maxygen, Inc. Targeting of genetic vaccine vectors
AUPP221098A0 (en) 1998-03-06 1998-04-02 Diatech Pty Ltd V-like domain binding molecules
CN1314911A (zh) 1998-05-01 2001-09-26 麦克西根股份有限公司 用dna改组优化害虫抗性基因
US6562622B1 (en) 1998-05-08 2003-05-13 Diatech Pty, Ltd Continuous in vitro evolution
JP2002517995A (ja) 1998-06-17 2002-06-25 マキシジェン， インコーポレイテッド 所望の特性を有するポリヌクレオチドを生成するための方法
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
JP2002522089A (ja) 1998-08-12 2002-07-23 マキシジェン， インコーポレイテッド 除草剤選択性作物を生成するためのｄｎａシャッフリング
US20020059659A1 (en) 1998-08-12 2002-05-16 Maxygen, Inc. DNA shuffling to produce herbicide selective crops
FR2782323B1 (fr) 1998-08-12 2002-01-11 Proteus Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues
WO2000012680A1 (en) 1998-08-31 2000-03-09 Maxygen, Inc. Transformation, selection, and screening of sequence-shuffled polynucleotides for development and optimization of plant phenotypes
AU1199000A (en) 1998-09-29 2000-04-17 Maxygen, Inc. Shuffling of codon altered genes
AU6510799A (en) 1998-10-07 2000-04-26 Maxygen, Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
EP1129184A1 (en) 1998-11-10 2001-09-05 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimation of plant phenotypes
BR9915191A (pt) 1998-11-10 2001-12-11 Maxygen Inc Métodos para obter um polinucleotìdeo isolado epara produzir uma célula recombinante,protoplasto de célula vegetal, coleção deprotoplastos de célula vegetal, vegetal,polinucleotìdeo, biblioteca, um ou mais membros debiblioteca e célula recombinante alvo
WO2000028017A1 (en) 1998-11-10 2000-05-18 Maxygen, Inc. Modified phosphoenolpyruvate carboxylase for improvement and optimization of plant phenotypes
FR2786787B1 (fr) 1998-12-08 2002-04-19 Proteus Methode d'analyse in vitro d'un phenotype connu a partir d'un echantillon d'acides nucleiques
US6358712B1 (en) 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
AU2415200A (en) 1999-01-18 2000-08-01 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
JP2002534965A (ja) 1999-01-19 2002-10-22 マキシジェン， インコーポレイテッド 所望の特徴を有する文字ストリング、ポリヌクレオチドおよびポリペプチドを作製するための方法
AU3391900A (en) 1999-03-05 2000-09-21 Maxygen, Inc. Encryption of traits using split gene sequences
JP2004500019A (ja) 1999-03-26 2004-01-08 ディベルサ　コーポレーション 定方向進化におけるエキソヌクレアーゼ仲介核酸再集合
AU4211600A (en) 1999-04-10 2000-11-14 Maxygen, Inc. Modified lipid production
AU4456200A (en) 1999-04-13 2000-11-14 Maxygen, Inc. Modified starch metabolism enzymes and encoding genes for improvement and optimization of plant phenotypes
AU5030900A (en) 1999-05-21 2000-12-12 Penn State Research Foundation, The Construction of incremental truncation libraries
US7332308B1 (en) 1999-05-21 2008-02-19 The Penn State Research Foundation Incrementally truncated nucleic acids and methods of making same
JP2003509010A (ja) 1999-05-31 2003-03-11 ノボザイムス アクティーゼルスカブ ペプチドのスクリーニング方法
EP1196552A2 (en) 1999-06-25 2002-04-17 Maxygen, Inc. Methods and compositions for engineering of attenuated vaccines
WO2001002865A1 (en) 1999-07-06 2001-01-11 Maxygen, Inc. Cyclotron mass spectrometry screening
AU5805500A (en) 1999-07-07 2001-01-30 Maxygen Aps A method for preparing modified polypeptides
AU6634300A (en) 1999-08-12 2001-03-13 Maxygen, Inc. Dna shuffling of dioxygenase genes for production of industrial chemicals
AU8001300A (en) 1999-10-07 2001-05-10 Maxygen, Inc. Ifn-alpha homologues
MXPA02003754A (es) 1999-10-13 2003-10-14 Univ Leland Stanford Junior Biosintesis de sustratos de policetido sintasa.
AU1456101A (en) 1999-11-03 2001-05-14 Maxygen, Inc. Antibody diversity generation
DE19953854C2 (de) 1999-11-09 2002-01-17 Max Planck Gesellschaft Verfahren zur Herstellung von Biopolymeren mit veränderten Eigenschaften
WO2001038513A2 (en) 1999-11-23 2001-05-31 Maxygen, Inc. Shuffling of agrobacterium and viral genes, plasmids and genomes for improved plant transformation
AU1799801A (en) 1999-11-23 2001-06-04 Maxygen, Inc. Homologous recombination in plants
WO2001042455A1 (en) 1999-12-08 2001-06-14 California Institute Of Technology Directed evolution of biosynthetic and biodegration pathways
US20020042055A1 (en) 1999-12-23 2002-04-11 Affholter Joseph A. Alteration of hydrolase genes and screening of the resulting libraries for the ability to catalyze specific reactions
SG121902A1 (en) 2000-01-11 2006-05-26 Maxygen Inc Integrated systems for diversity generation and screening
US6329178B1 (en) 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
AU2001241939A1 (en) 2000-02-28 2001-09-12 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
EP1268815A2 (en) 2000-02-29 2003-01-02 Maxygen, Inc. Triazine degrading enzymes
AU2001269676A1 (en) 2000-03-02 2001-09-24 Maxygen, Inc. Enzymes, pathways and organisms for making a polymerizable monomer by whole cellbioprocess
US20010044111A1 (en) 2000-03-20 2001-11-22 Brian Carr Method for generating recombinant DNA molecules in complex mixtures
EP1276861A2 (en) 2000-03-24 2003-01-22 Maxygen, Inc. Methods for modulating cellular and organismal phenotypes
AU4964101A (en) 2000-03-30 2001-10-15 Maxygen Inc In silico cross-over site selection
AU2001249811B2 (en) 2000-04-03 2006-06-08 Maxygen, Inc. Subtilisin variants
WO2001096551A2 (en) 2000-06-14 2001-12-20 Diversa Corporation Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating
AU2001271912A1 (en) 2000-07-07 2002-01-21 Maxygen, Inc. Molecular breeding of transposable elements
EP1354031A2 (en) 2000-07-31 2003-10-22 Maxygen, Inc. Nucleotide incorporating enzymes
FR2813314B1 (fr) 2000-08-25 2004-05-07 Biomethodes Procede de mutagenese dirigee massive
WO2002022663A2 (en) 2000-09-18 2002-03-21 Maxygen, Inc. Stress resistant retroviruses
WO2002029032A2 (en) 2000-09-30 2002-04-11 Diversa Corporation Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating
US7560622B2 (en) 2000-10-06 2009-07-14 Pioneer Hi-Bred International, Inc. Methods and compositions relating to the generation of partially transgenic organisms
JP3777158B2 (ja) * 2000-11-10 2006-05-24 アミコージェン・インコーポレイテッド 一方向性の一本鎖ｄｎａ切片を用いた組換えｄｎａライブラリーの製造方法
PT1341909E (pt) * 2000-12-12 2005-10-31 Alligator Bioscience Ab Metodo para a evolucao molecular in vitro da funcao de proteina
US6958213B2 (en) * 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function

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DE60312507T3 (de) 2011-08-18
ES2285118T3 (es) 2007-11-16
DK1504098T3 (da) 2007-06-11
EP1504098B2 (en) 2011-02-09
CA2485506C (en) 2012-02-28
JP2005525820A (ja) 2005-09-02
AU2003230027A8 (en) 2003-12-02
WO2003097834A2 (en) 2003-11-27
JP4372679B2 (ja) 2009-11-25
ATE356871T1 (de) 2007-04-15
US7262012B2 (en) 2007-08-28
DE60312507T2 (de) 2007-12-06
WO2003097834A3 (en) 2004-02-19
AU2003230027A1 (en) 2003-12-02
ES2285118T5 (es) 2012-09-21
DK200401821A (da) 2004-11-23
CA2485506A1 (en) 2003-11-27
DE60312507D1 (de) 2007-04-26
EP1504098A2 (en) 2005-02-09
US20060166198A1 (en) 2006-07-27

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