ATE354673T1 - Direkte quantifizierung von gering vorkommender rns - Google Patents

Direkte quantifizierung von gering vorkommender rns

Info

Publication number
ATE354673T1
ATE354673T1 AT98938408T AT98938408T ATE354673T1 AT E354673 T1 ATE354673 T1 AT E354673T1 AT 98938408 T AT98938408 T AT 98938408T AT 98938408 T AT98938408 T AT 98938408T AT E354673 T1 ATE354673 T1 AT E354673T1
Authority
AT
Austria
Prior art keywords
rna
abound
low
direct quantification
hybrid
Prior art date
Application number
AT98938408T
Other languages
English (en)
Inventor
Jill M Kolesar
Peter G Allen
Original Assignee
Wisconsin Alumni Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found
Application granted granted Critical
Publication of ATE354673T1 publication Critical patent/ATE354673T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AT98938408T 1997-08-05 1998-08-05 Direkte quantifizierung von gering vorkommender rns ATE354673T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/906,443 US6013442A (en) 1997-08-05 1997-08-05 Direct quantitation of low copy number RNA

Publications (1)

Publication Number Publication Date
ATE354673T1 true ATE354673T1 (de) 2007-03-15

Family

ID=25422458

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98938408T ATE354673T1 (de) 1997-08-05 1998-08-05 Direkte quantifizierung von gering vorkommender rns

Country Status (8)

Country Link
US (1) US6013442A (de)
EP (1) EP0990048B9 (de)
JP (1) JP2001512699A (de)
AT (1) ATE354673T1 (de)
AU (1) AU8693798A (de)
CA (1) CA2298884C (de)
DE (1) DE69837146T2 (de)
WO (1) WO1999007894A1 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261781B1 (en) * 1997-08-05 2001-07-17 Wisconsin Alumni Research Foundation Direct detection and mutation analysis of low copy number nucleic acids
AU1526400A (en) * 1998-11-23 2000-06-19 Exact Laboratories, Inc. Method for detecting target sequences in small proportions in heterogeneous samples
US6420115B1 (en) 1999-12-21 2002-07-16 Ingeneus Corporation Cation mediated triplex hybridization assay
US6858390B2 (en) 1998-12-31 2005-02-22 Ingeneus Corporation Aptamers containing sequences of nucleic acid or nucleic acid analogues bound homologously, or in novel complexes
US6656692B2 (en) 1999-12-21 2003-12-02 Ingeneus Corporation Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US6403313B1 (en) * 1999-12-21 2002-06-11 Ingeneus Corporation Fluorescent intensity assay for duplex and triplex nucleic acid hybridization solution utilizing fluorescent intercalators
DE60029092T2 (de) 1999-04-09 2007-02-01 Exact Sciences Corp., Marlborough Verfahren zur detektion von nukleinsäuren, welche auf krebs hinweisen
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
DE60043896D1 (de) 1999-12-07 2010-04-08 Exact Sciences Corp Verfahren zum nachweis von lungenneoplasmen in fäkalen proben
US6911536B1 (en) 1999-12-21 2005-06-28 Ingeneus Corporation Triplex and quadruplex catalytic hybridization
US20030181412A1 (en) * 1999-12-21 2003-09-25 Ingeneus Corporation Method for modifying transcription and/or translation in an organism for therapeutic, prophylactic and/or analytic uses
US6927027B2 (en) 1999-12-21 2005-08-09 Ingeneus Corporation Nucleic acid multiplex formation
US7309569B2 (en) 1999-12-21 2007-12-18 Ingeneus, Inc. Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US7052844B2 (en) 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
US6982147B2 (en) * 2000-01-24 2006-01-03 Ingeneus Corporation Apparatus for assaying biopolymer binding by means of multiple measurements under varied conditions
US20030170659A1 (en) * 2000-01-24 2003-09-11 Ingeneus Corporation Electrical treatment of binding media to encourage, discourage and/or study biopolymer binding
US6613524B1 (en) 2000-01-24 2003-09-02 Ingeneus Corporation Amperometric affinity assay and electrically stimulated complexes of nucleic acids
US7220541B2 (en) 2000-01-24 2007-05-22 Ingeneus, Inc. Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions
US6265170B1 (en) 2000-01-24 2001-07-24 Ingeneus Corporation Homogenous assay of duplex of triplex hybridization by means of multiple measurements under varied conditions
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
EP1330547A4 (de) * 2000-10-04 2005-01-19 Univ Leland Stanford Junior Renaturierung, reassoziierung, assoziierung und hybridisierung von nukleinsäuremolekülen
KR100386606B1 (ko) * 2001-02-03 2003-06-02 엘지전자 주식회사 Ｄｎａ 검출 방법 및 그 장치
KR100459394B1 (ko) * 2001-10-30 2004-12-03 엘지전자 주식회사 인터컬레이터를 이용한 핵산의 전기화학발광 검출방법
AU2003300569A1 (en) * 2003-11-17 2005-06-08 Agilent Technologies, Inc. Nucleic acid detection
US7582430B2 (en) * 2004-01-20 2009-09-01 United States Of America As Represented By The Secretary Of The Army Immunoliposome-nucleic acid amplification (ILNAA) assay
WO2005098031A1 (en) * 2004-04-06 2005-10-20 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Liquid-based hybridization assay
US20100193357A1 (en) * 2009-01-30 2010-08-05 Chang Po-Ling Quantitative analysis method for micrornas

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8902689D0 (en) * 1989-02-07 1989-03-30 Ici Plc Assay method
JP2833063B2 (ja) * 1989-10-31 1998-12-09 株式会社島津製作所 核酸の検出法
WO1993013223A1 (en) * 1991-12-23 1993-07-08 Chiron Corporation Hiv probes for use in solution phase sandwich hybridization assays
JP3189000B2 (ja) * 1994-12-01 2001-07-16 東ソー株式会社 特定核酸配列の検出方法

Also Published As

Publication number Publication date
DE69837146T2 (de) 2007-10-31
AU8693798A (en) 1999-03-01
EP0990048B9 (de) 2007-05-23
US6013442A (en) 2000-01-11
CA2298884C (en) 2007-10-23
EP0990048B1 (de) 2007-02-21
CA2298884A1 (en) 1999-02-18
WO1999007894A1 (en) 1999-02-18
JP2001512699A (ja) 2001-08-28
DE69837146D1 (de) 2007-04-05
EP0990048A1 (de) 2000-04-05

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