ATE364088T1 - Verfahren zur herstellung von pharmazeutisch aktiver, rekombinanter urokinase umfassend die verwendung von buttersäure oder deren salze - Google Patents

Verfahren zur herstellung von pharmazeutisch aktiver, rekombinanter urokinase umfassend die verwendung von buttersäure oder deren salze

Info

Publication number
ATE364088T1
ATE364088T1 AT02005935T AT02005935T ATE364088T1 AT E364088 T1 ATE364088 T1 AT E364088T1 AT 02005935 T AT02005935 T AT 02005935T AT 02005935 T AT02005935 T AT 02005935T AT E364088 T1 ATE364088 T1 AT E364088T1
Authority
AT
Austria
Prior art keywords
salts
pharmaceutically active
culture medium
active recombinant
producing pharmaceutically
Prior art date
Application number
AT02005935T
Other languages
English (en)
Inventor
Achille Arini
Raffaella Coppolecchia
Francesca Pagani
Detlev Herbst
Antonio Tognini
Original Assignee
Cerbios Pharma Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cerbios Pharma Sa filed Critical Cerbios Pharma Sa
Application granted granted Critical
Publication of ATE364088T1 publication Critical patent/ATE364088T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Hematology (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT02005935T 2001-03-16 2002-03-15 Verfahren zur herstellung von pharmazeutisch aktiver, rekombinanter urokinase umfassend die verwendung von buttersäure oder deren salze ATE364088T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/815,533 US20030040095A1 (en) 2001-03-16 2001-03-16 Method for the production of pharmaceutically active recombinant proteins

Publications (1)

Publication Number Publication Date
ATE364088T1 true ATE364088T1 (de) 2007-06-15

Family

ID=25218082

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02005935T ATE364088T1 (de) 2001-03-16 2002-03-15 Verfahren zur herstellung von pharmazeutisch aktiver, rekombinanter urokinase umfassend die verwendung von buttersäure oder deren salze

Country Status (9)

Country Link
US (1) US20030040095A1 (de)
EP (1) EP1245681B1 (de)
AT (1) ATE364088T1 (de)
AU (1) AU772144B2 (de)
CA (1) CA2376131A1 (de)
DE (1) DE60220469T2 (de)
ES (1) ES2288527T3 (de)
RU (2) RU2227745C2 (de)
ZA (1) ZA200202136B (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PE20070796A1 (es) * 2005-10-24 2007-08-15 Wyeth Corp Metodo de produccion proteica utilizando compuestos anti-senescencia
TW200902708A (en) * 2007-04-23 2009-01-16 Wyeth Corp Methods of protein production using anti-senescence compounds
HUE061746T2 (hu) 2007-07-09 2023-08-28 Genentech Inc Diszulfidkötés redukciójának megelõzése polipeptidek rekombináns elõállítása során
US20090023186A1 (en) * 2007-07-22 2009-01-22 Excellgene Sa Use of valproic acid for enhancing production of recombinant proteins in mammalian cells
DK2574677T3 (da) * 2007-12-27 2017-11-06 Baxalta GmbH Fremgangsmåder til celledyrkning
EP3219795B1 (de) * 2008-01-18 2024-03-13 BioMarin Pharmaceutical Inc. Herstellung von aktiven hoch phosphorylierten humanen lysosomalen sulfataseenzymen und verwendungen davon
IT202300008805A1 (it) 2023-05-04 2024-11-04 Cerbios Pharma Sa Metodo per la produzione di urochinasi ricombinante umana a basso peso molecolare farmaceuticamente attiva

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2387242A1 (fr) * 1977-04-12 1978-11-10 Choay Sa Compositions purifiees d'urokinase et procede pour leur obtention
GR78180B (de) * 1982-04-15 1984-09-26 Genentech Inc
BE900826A (fr) * 1984-10-16 1985-04-16 Ucb Sa Procede de preparation d'un clone d'escherichia coli produisant de la preprourokinase humaine.
DE3439980A1 (de) * 1984-11-02 1986-05-07 Behringwerke Ag, 3550 Marburg Verfahren zur reinigung sowie pasteurisierung von urokinase
JPS6384490A (ja) * 1986-09-29 1988-04-15 Green Cross Corp:The プラスミノ−ゲンアクチベ−タ−前駆体の産生増強方法
DE4101736A1 (de) * 1991-01-22 1992-07-23 Gruenenthal Gmbh Neue als plasminogenaktivatoren einsetzbare polypeptide, dafuer codierende plasmide und verfahren zu deren herstellung und deren verwendung
US5705364A (en) * 1995-06-06 1998-01-06 Genentech, Inc. Mammalian cell culture process
WO2000065070A2 (en) * 1999-04-26 2000-11-02 Genentech, Inc. Cell culture process for glycoproteins

Also Published As

Publication number Publication date
RU2259212C2 (ru) 2005-08-27
DE60220469T2 (de) 2008-01-10
EP1245681A2 (de) 2002-10-02
DE60220469D1 (de) 2007-07-19
RU2227745C2 (ru) 2004-04-27
CA2376131A1 (en) 2002-09-16
EP1245681B1 (de) 2007-06-06
ES2288527T3 (es) 2008-01-16
ZA200202136B (en) 2002-06-21
AU772144B2 (en) 2004-04-08
EP1245681A3 (de) 2003-12-03
US20030040095A1 (en) 2003-02-27
RU2003125917A (ru) 2005-02-27
AU2460702A (en) 2002-09-19

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