ATE366317T1 - Herstellung von peptiden und proteinen durch anhäufung in vom endoplasmatischen reticulum abgeleiteten proteinkörpern - Google Patents

Herstellung von peptiden und proteinen durch anhäufung in vom endoplasmatischen reticulum abgeleiteten proteinkörpern

Info

Publication number
ATE366317T1
ATE366317T1 AT02807566T AT02807566T ATE366317T1 AT E366317 T1 ATE366317 T1 AT E366317T1 AT 02807566 T AT02807566 T AT 02807566T AT 02807566 T AT02807566 T AT 02807566T AT E366317 T1 ATE366317 T1 AT E366317T1
Authority
AT
Austria
Prior art keywords
peptides
proteins
accumulation
production
protein body
Prior art date
Application number
AT02807566T
Other languages
English (en)
Inventor
Mugica Maria Ludevid
Quetglas Margarita Torrent
Sabine Lasserre-Ramassamy
Original Assignee
Era Plantech S L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Era Plantech S L filed Critical Era Plantech S L
Application granted granted Critical
Publication of ATE366317T1 publication Critical patent/ATE366317T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/425—Zeins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
AT02807566T 2002-06-28 2002-08-05 Herstellung von peptiden und proteinen durch anhäufung in vom endoplasmatischen reticulum abgeleiteten proteinkörpern ATE366317T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200201508A ES2224792B1 (es) 2002-06-28 2002-06-28 Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.

Publications (1)

Publication Number Publication Date
ATE366317T1 true ATE366317T1 (de) 2007-07-15

Family

ID=29797406

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02807566T ATE366317T1 (de) 2002-06-28 2002-08-05 Herstellung von peptiden und proteinen durch anhäufung in vom endoplasmatischen reticulum abgeleiteten proteinkörpern

Country Status (16)

Country Link
US (2) US7575898B2 (de)
EP (1) EP1523558B1 (de)
JP (2) JP4610334B2 (de)
KR (2) KR100987946B1 (de)
CN (1) CN100460510C (de)
AR (1) AR040293A1 (de)
AT (1) ATE366317T1 (de)
AU (1) AU2002333330B2 (de)
CA (1) CA2490348A1 (de)
DE (1) DE60221051T2 (de)
DK (1) DK1523558T3 (de)
ES (2) ES2224792B1 (de)
MX (1) MXPA05000290A (de)
RU (1) RU2381275C2 (de)
UY (1) UY27864A1 (de)
WO (1) WO2004003207A1 (de)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL155588A0 (en) * 2003-04-27 2003-11-23 Metabogal Ltd Methods for expression of enzymatically active recombinant lysosomal enzymes in transgenic plant root cells and vectors used thereby
US20050032211A1 (en) * 1996-09-26 2005-02-10 Metabogal Ltd. Cell/tissue culturing device, system and method
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
US7951557B2 (en) 2003-04-27 2011-05-31 Protalix Ltd. Human lysosomal proteins from plant cell culture
GB0426160D0 (en) * 2004-11-29 2004-12-29 Era Plantech S L Production of proteins
GB0426161D0 (en) * 2004-11-29 2004-12-29 Era Plantech S L Protein isolation and purification
WO2006095749A1 (ja) * 2005-03-07 2006-09-14 National University Corporation Nagoya University 植物でのペプチドの発現・集積方法
US8163880B2 (en) * 2006-02-23 2012-04-24 Era Biotech S.A. Production of biologically active proteins
US7732569B2 (en) * 2006-10-19 2010-06-08 E.I. Du Pont De Nemours And Company Zein-based peptide tags for the expression and purification of bioactive peptides
AU2008246928B2 (en) 2007-05-07 2014-04-17 Protalix Ltd. Large scale disposable bioreactor
WO2009076778A1 (en) 2007-11-27 2009-06-25 Medicago Inc. Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
AU2009267769B2 (en) * 2008-07-08 2015-07-16 Medicago Inc. Soluble recombinant influenza antigens
EP2356133B1 (de) 2008-10-10 2019-07-31 Zera Intein Protein Solutions, S.L. Rekombinante proteinkörper als immunogenspezifische adjuvansien
HUE039100T2 (hu) 2009-06-24 2018-12-28 Medicago Inc Hemagglutinint tartalmazó kimer influenzavírus-jellegû részecskék
RU2415936C1 (ru) * 2010-01-11 2011-04-10 Учреждение Российской академии наук Институт химической биологии и фундаментальной медицины Сибирского отделения РАН (ИХБФМ СО РАН) Способ секреции зеленого флуоресцентного белка (gfp) из растительных клеток
EP2418284A1 (de) * 2010-08-13 2012-02-15 ERA Biotech, S.A. Polypeptidsequenzen zur Induzierung von Proteinkörpern
EP2468871A1 (de) 2010-12-23 2012-06-27 Philip Morris Products S.A. Verfahren zur Produktion von Apolipoprotein in Pflanzen
EP2468870A1 (de) * 2010-12-23 2012-06-27 Philip Morris Products S.A. Verfahren zur Expression von Desoxyribonuklease in Pflanzen
EP2468872A1 (de) * 2010-12-23 2012-06-27 Philip Morris Products S.A. Verfahren zur Produktion von Müllerian-Inhibitor-Substanz in Pflanzen
PT2665818T (pt) 2011-01-17 2017-07-05 Philip Morris Products Sa Expressão de proteínas em plantas
FI20115704A7 (fi) 2011-07-01 2013-01-02 Teknologian Tutkimuskeskus Vtt Oy Vierasproteiinien tuoton parantaminen
US9603907B2 (en) 2012-02-01 2017-03-28 Protalix Ltd. Dry powder formulations of dNase I
WO2016090345A1 (en) * 2014-12-05 2016-06-09 Planet Biotechnology Inc. Dpp4 immunoadhesin compositions and methods
DE102015107846A1 (de) 2015-05-19 2016-11-24 Johann Wolfgang Goethe-Universität Frankfurt Synthetische Organellen in Hefe
KR101848082B1 (ko) * 2017-05-11 2018-04-12 주식회사 바이오앱 셀룰로오즈 결합 도메인을 포함하는 재조합 벡터 및 상기 벡터를 사용하여 단백질을 분리정제하는 방법
AR111837A1 (es) 2017-05-30 2019-08-21 Agrivida Inc Plantas transgénicas inmunomoduladoras y métodos relacionados
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
AU2021353004A1 (en) 2020-09-30 2023-04-13 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
CN113683669B (zh) * 2021-03-25 2023-05-16 中国农业大学 基于与醇溶蛋白的互作在植物中积累目标蛋白的方法

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4215040A (en) * 1978-12-22 1980-07-29 The Procter & Gamble Company Density separation process
DE3855591T2 (de) * 1987-10-20 1997-04-03 Plant Genetic Systems Nv Verfahren zur Herstellung eines biologisch-aktiven Peptides durch Expression von modifizierten Speicherproteingenen in transgenetischen Pflanzen
AU2811889A (en) 1987-10-20 1989-05-23 Plant Genetic Systems N.V. A process for the production of biologically active peptide via the expression of modified storage seed protein genes in transgenic plants
US5589615A (en) 1988-10-14 1996-12-31 Plant Genetic Systems N.V. Process for the production of transgenic plants with increased nutritional value via the expression of modified 2S storage albumins
US5571697A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Texas Medical Center Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus
US5948682A (en) * 1991-02-22 1999-09-07 Sembiosys Genetics Inc. Preparation of heterologous proteins on oil bodies
US5650554A (en) 1991-02-22 1997-07-22 Sembiosys Genetics Inc. Oil-body proteins as carriers of high-value peptides in plants
MX9703017A (es) * 1994-10-24 1997-10-31 Texas A & M Univ Sys Inmunizacion oral con plantas transgenicas.
US5935822A (en) 1995-03-21 1999-08-10 The Regents Of The University Of Colorado Product and process for membrane and soluble polypeptide segregation
FR2744134B1 (fr) 1996-01-29 1998-04-03 Biocem Proteines de reserve de plantes enrichies en acides amines, notamment alpha-zeine de mais enrichie en lysine plantes exprimant ces proteines
US5990384A (en) 1996-05-31 1999-11-23 New Mexico State University Co-expression of proteins
JP2001518305A (ja) * 1997-09-30 2001-10-16 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 植物種子におけるタンパク質製造
AR020090A1 (es) 1998-06-15 2002-04-10 John Innes Ct Celula vegetal monocotiledonea o semilla de ella,planta o parte de una planta, metodo para hacer una celula vegetal monocotiledonea, metodo para hacer unaplanta, uso de una construccion de expresion para producir una celula de planta o semilla monocotiledonea, y uso de una construccion de expresion
EP1141347A1 (de) * 1998-12-31 2001-10-10 New Mexico State University Technology Transfer Corporation Coexpression von proteinen
US20020061309A1 (en) * 2000-03-08 2002-05-23 Garger Stephen J. Production of peptides in plants as N-terminal viral coat protein fusions
US7417178B2 (en) 2000-05-02 2008-08-26 Ventria Bioscience Expression of human milk proteins in transgenic plants
WO2002086077A2 (en) 2001-04-18 2002-10-31 New Mexico State University Technology Transfer Corporation Co-expression of zein proteins
JP3940793B2 (ja) 2002-05-15 2007-07-04 独立行政法人農業生物資源研究所 任意のペプチドを植物のタンパク顆粒で蓄積させる方法
DK1521767T3 (da) * 2002-05-24 2010-04-12 Medtronic Inc Fremgangsmåder og konstrukter til ekspression af clostripain med højt udbytte
ES2224792B1 (es) 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
US20070150976A1 (en) 2003-12-09 2007-06-28 Ventria Bioscience High-level expression of fusion polypeptides in plant seeds utilizing seed-storage proteins as fusion carriers
BRPI0508518A (pt) 2004-03-08 2007-08-14 Syngenta Participations Ag proteìna e promotor de semente de milho rica em glutamina
WO2005113775A1 (en) 2004-05-21 2005-12-01 Simon Fraser University Pproduction of a protein localized to the endoplasmic reticulum protein bodies in a transgenic plant and seed
GB0426160D0 (en) 2004-11-29 2004-12-29 Era Plantech S L Production of proteins
GB0426161D0 (en) 2004-11-29 2004-12-29 Era Plantech S L Protein isolation and purification
US8337817B2 (en) * 2006-12-26 2012-12-25 Shin Nippon Biomedical Laboratories, Ltd. Preparation for transnasal application

Also Published As

Publication number Publication date
CA2490348A1 (en) 2004-01-08
UY27864A1 (es) 2003-09-30
RU2005101738A (ru) 2006-07-20
RU2381275C2 (ru) 2010-02-10
KR20100017491A (ko) 2010-02-16
ES2224792B1 (es) 2007-02-16
DE60221051D1 (de) 2007-08-16
WO2004003207A1 (en) 2004-01-08
DK1523558T3 (da) 2007-11-05
CN1639342A (zh) 2005-07-13
ES2289185T3 (es) 2008-02-01
MXPA05000290A (es) 2005-03-31
EP1523558A1 (de) 2005-04-20
US20040005660A1 (en) 2004-01-08
CN100460510C (zh) 2009-02-11
AU2002333330B2 (en) 2007-07-12
JP4610334B2 (ja) 2011-01-12
AU2002333330A1 (en) 2004-01-19
JP2005531309A (ja) 2005-10-20
US7575898B2 (en) 2009-08-18
US20100083403A1 (en) 2010-04-01
JP2010213713A (ja) 2010-09-30
EP1523558B1 (de) 2007-07-04
DE60221051T2 (de) 2008-03-13
WO2004003207A9 (en) 2005-02-10
KR20050024419A (ko) 2005-03-10
KR100987946B1 (ko) 2010-10-18
US8802825B2 (en) 2014-08-12
ES2224792A1 (es) 2005-03-01
AR040293A1 (es) 2005-03-23

Similar Documents

Publication Publication Date Title
ATE366317T1 (de) Herstellung von peptiden und proteinen durch anhäufung in vom endoplasmatischen reticulum abgeleiteten proteinkörpern
EP1496926A4 (de) Verwendung von transthyretin-peptid/protein-fusionen zur verlängerung der serumhalbwertszeit von pharmakologisch wirksamen peptiden/proteinen
WO2004078777A3 (en) Dipeptidyl-peptidase protected proteins
ATE517119T1 (de) Einzelketteninsulin
EP1490483A4 (de) In-vivo-einbau von nicht in der natur vorkommenden aminosäuren
DE602004029015D1 (de) Hochkonzentrierter antikörper und proteinformulierungen
WO2007064917A3 (en) Methods of preparation of recombinant forms of human beta-amyloid protein and uses of these proteins
EP1545600A4 (de) Protein- und peptid-expression für passive immunität
DE60328469D1 (de) Aminosäuren mit affinität zu alpha-2-delta-protein
DK2380583T3 (da) Små peptider til behandling af Alzheimers sygdom og andre beta-amyloidprotein-fibrillogeneseforstyrrelser
WO2004074486A3 (en) Fusion proteins of interferon alpha muteins with improved properties
IL148401A0 (en) Hsp70-derived peptides and uses thereof in the diagnosis and treatment of autoimmune diseases
ATE412745T1 (de) Antiangiogene peptide vom n-terminus von endostatin
WO2003014078A3 (en) Muc7d1 peptides as antifungal and antibacterial agents
DE60216523D1 (de) Herstellung und verwendung von mit verzweigten aminosäuren angereicherten sojaproteinhydrolysaten
ATE400581T1 (de) Peptid-antioxidantien aus sojaproteinen
WO2008068428A3 (fr) Utilisation cosmétique de protéines de type chitinase
WO2002073373A3 (en) Ensemble-based strategy for the design of protein pharmaceuticals
JP2005535281A5 (de)
EP1432448A4 (de) Neue moleküle der familie mit hkid-1 verwandter proteine und verwendungen davon
EP1411964A4 (de) Die blutgerinnung modulierende peptide und ihre verwendungszwecke
WO2004053117A3 (en) Serine protease
EP2327723A3 (de) Proteinbasierte TNF-alpha-Varianten zur Behandlung von mit TNF-alpha zusammenhängenden Erkrankungen
DE60217711D1 (de) Ptp10d nukleinsäuren und peptide in der regulation von energie-homeostase
ATE506433T1 (de) Prozessierung von peptiden und proteinen

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties