ATE366822T1 - Verfahren zum quantitativen nachweis von nukleinsäuren - Google Patents

Verfahren zum quantitativen nachweis von nukleinsäuren

Info

Publication number
ATE366822T1
ATE366822T1 AT99956010T AT99956010T ATE366822T1 AT E366822 T1 ATE366822 T1 AT E366822T1 AT 99956010 T AT99956010 T AT 99956010T AT 99956010 T AT99956010 T AT 99956010T AT E366822 T1 ATE366822 T1 AT E366822T1
Authority
AT
Austria
Prior art keywords
nucleic acids
quantitative detection
calibrator
probes
nucleic acid
Prior art date
Application number
AT99956010T
Other languages
English (en)
Inventor
Giuseppe Locatelli
Paolo Lusso
Mauro Malnati
Francesca Salvatori
Gabriella Scarlatti
Original Assignee
Paolo Lusso
Mauro Malnati
Gabriella Scarlatti
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Paolo Lusso, Mauro Malnati, Gabriella Scarlatti filed Critical Paolo Lusso
Application granted granted Critical
Publication of ATE366822T1 publication Critical patent/ATE366822T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • AIDS & HIV (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT99956010T 1998-11-17 1999-11-17 Verfahren zum quantitativen nachweis von nukleinsäuren ATE366822T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT1998MI002491A IT1303767B1 (it) 1998-11-17 1998-11-17 Metodo di quantificazione di acidi nucleici.

Publications (1)

Publication Number Publication Date
ATE366822T1 true ATE366822T1 (de) 2007-08-15

Family

ID=11381075

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99956010T ATE366822T1 (de) 1998-11-17 1999-11-17 Verfahren zum quantitativen nachweis von nukleinsäuren

Country Status (9)

Country Link
US (2) US7029843B1 (de)
EP (1) EP1131466B1 (de)
JP (1) JP2002530090A (de)
AT (1) ATE366822T1 (de)
AU (1) AU1272000A (de)
CA (1) CA2351740A1 (de)
DE (1) DE69936532D1 (de)
IT (1) IT1303767B1 (de)
WO (1) WO2000029613A1 (de)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8012719B2 (en) * 1998-11-17 2011-09-06 Paolo Lusso Method for the quantitative detection of nucleic acids
JP2002125687A (ja) * 2000-10-30 2002-05-08 Tosoh Corp Ｈｉｖ−１検出のためのオリゴヌクレオチドおよび検出法
US20020187488A1 (en) * 2001-01-30 2002-12-12 Chao Lin Quantitative assay for nucleic acids
EP1236804A1 (de) 2001-03-02 2002-09-04 Boehringer Mannheim Gmbh Verfahren zur Bestimmung von Nukleinsäuren mittels einer Kontrolle
ES2305144T3 (es) * 2001-03-02 2008-11-01 F. Hoffmann-La Roche Ag Metodo para la determinacion de un acido nucleico utilizando un control.
ATE412777T1 (de) * 2002-03-04 2008-11-15 Us Army Med Res Mat Command Interne positive kontrolle für auf sonden basierende nukleinsäuremolekül-assays und verfahren zur herstellung und verwendung davon
US7052848B2 (en) 2002-03-04 2006-05-30 The United States Of America As Represented By The Secretary Of The Army Internal positive control for probe-based nucleic acid molecule assays and methods of making and using thereof
ATE454470T1 (de) * 2002-07-26 2010-01-15 Abbott Lab Verfahren zum nachweis und zur quantifizierung von hepatitis-c-virus
US7396646B2 (en) * 2003-01-22 2008-07-08 Modular Genetics, Inc. Alien sequences
EP1625232B1 (de) * 2003-05-16 2013-09-04 GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Nukleinsäuremolekül als interne kontrolle für nukleinsäureamplifikationssysteme
ES2375406T3 (es) * 2004-04-26 2012-02-29 Wako Pure Chemical Industries, Ltd. Sonda y cebador para la detección de bacilos tuberculosos, y procedimiento para la detección de bacilos tuberculosos humanos con el uso de los mismos.
US20060110724A1 (en) 2004-11-19 2006-05-25 Burkhardt William Iii Quantitative real-time assay for noroviruses and enteroviruses with built in quality control standard
JP2008182920A (ja) * 2007-01-29 2008-08-14 Sony Corp 蛍光標識オリゴヌクレオチドと該オリゴヌクレオチドを利用する方法
US20080274458A1 (en) * 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
US8366652B2 (en) 2007-08-17 2013-02-05 The Invention Science Fund I, Llc Systems, devices, and methods including infection-fighting and monitoring shunts
JP5223267B2 (ja) * 2007-08-23 2013-06-26 東レ株式会社 遺伝子増幅産物の回収方法および遺伝子増幅産物の回収キット
EP2141246A1 (de) * 2008-07-01 2010-01-06 Koninklijke Philips Electronics N.V. Spaltungslose Verfahren zur PCR-Erkennung mithilfe von SERRS
US20110295088A1 (en) 2008-12-04 2011-12-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Systems, devices, and methods including implantable devices with anti-microbial properties
EP2384168B1 (de) 2008-12-04 2014-10-08 Searete LLC Aktiv kontrollierbare sterilisierende erregungsabgabe-implantate
JP2012531908A (ja) * 2009-07-03 2012-12-13 エイジェンシー フォー サイエンス，テクノロジー アンド リサーチ 結核菌を検出するための方法および／またはプライマー
US8877464B2 (en) 2010-07-29 2014-11-04 Roche Molecular Systems, Inc. Qualitative and quantitative detection of microbial nucleic acids
CN102154515A (zh) * 2011-03-18 2011-08-17 武汉大学 人类疱疹病毒6型实时荧光定量pcr试剂盒
WO2012153153A1 (en) 2011-05-11 2012-11-15 Diagon Kft. Procedure for rapid determination of viruses using nucleic acid-based molecular diagnostics, and a kit for this purpose
US9101678B2 (en) 2011-11-03 2015-08-11 Elwha Llc Heat-sanitization of surfaces
CA2873370C (en) * 2012-07-16 2021-06-08 Kabushiki Kaisha Dnaform Nucleic acid probe, method for designing nucleic acid probe, and method for detecting target sequence
EP2891714B1 (de) 2012-08-30 2018-07-11 Kabushiki Kaisha Dnaform Verfahren zur analyse einer zielnukleinsäure, kit und analysevorrichtung

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5457027A (en) 1993-05-05 1995-10-10 Becton, Dickinson And Company Internal controls for isothermal nucleic acid amplification reactions
JPH10505224A (ja) 1994-06-10 1998-05-26 ジョージタウン、ユニバーシティー 組換えウイルスおよびその関連定量法
US6395470B2 (en) * 1997-10-31 2002-05-28 Cenetron Diagnostics, Llc Method for monitoring nucleic acid assays using synthetic internal controls with reversed nucleotide sequences

Also Published As

Publication number Publication date
EP1131466B1 (de) 2007-07-11
US7384769B2 (en) 2008-06-10
US20050239128A1 (en) 2005-10-27
US7029843B1 (en) 2006-04-18
EP1131466A1 (de) 2001-09-12
WO2000029613A1 (en) 2000-05-25
DE69936532D1 (de) 2007-08-23
CA2351740A1 (en) 2000-05-25
AU1272000A (en) 2000-06-05
ITMI982491A1 (it) 2000-05-17
JP2002530090A (ja) 2002-09-17
IT1303767B1 (it) 2001-02-23

Similar Documents

Publication Publication Date Title
ATE366822T1 (de) Verfahren zum quantitativen nachweis von nukleinsäuren
ATE206764T1 (de) Verfahren zum sensitiven nachweis von nukleinsäuren
DE68915202D1 (de) Verfahren zur Vermehrung und zum Nachweis von Nukleinsäuresequenzen.
ATE545710T1 (de) Verfahren zur vervielfältigung von nukleinsäuren
ATE243759T1 (de) Verfahren zur dämpfung der fluoreszene in lösung von fluoreszenzmarkierten oligonucleotidsonden
ATE238434T1 (de) Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren
DE3673501D1 (de) Mittel und verfahren zur funktionalisierung von nukleinsaeuren.
ATE190357T1 (de) Schnelles verfahren fur die dns sequenzierung und diagnostische anwendungen
DE3853678D1 (de) Verfahren zum nachweis des karzinogenen menschlichen papillomavirus.
ATE345682T1 (de) In vitro verfahren zum einbringen von nukleinsäuren in eine zelle
DE69520330D1 (de) Verfahren zum Nachweis von spezifischen Nukleinsäuresequenzen
DE3586629D1 (de) Nukleotidanaloga zur markierung von nukleinsaeuren und nachweis.
DE68918657D1 (de) Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen.
ES8609472A1 (es) Una sonda de deteccion de acidos nucleicos
DE69003104D1 (de) Verfahren zum nachweis von spezifischen sequenzen von nukleinsäuren und ihre verwendungen.
ATE353973T1 (de) Die verwendung der 3'-wesentlichen bearbeitungswirksamkeit von dns-polymerase
DE69635848D1 (de) Verfahren zum Nachweis von Ki-ras Mutationen
DE59510762D1 (de) Verfahren zum besonders sensitiven Nachweis von Nukleinsäuren
DE69332666D1 (de) Verfahren zur einfachen nukleotid-primer-verlängerung zum nachweis spezifischer allele und dafür geeigneter kit
ZA862334B (en) Process for amplifying,detecting and/or cloning nucleic acid sequences
EP0272098A3 (de) Verfahren zur Vergrösserung und zum Nachweis von RNS-Sequenzen
DE69823372T8 (de) Verfahren und reagentiensatz zur amplifizierung, sequenzierung und typisierung von hla i genen
EP0128018A3 (en) Molecular genetic probe, and method of forming same, assay technique and kit using said molecular genetic probe
DE59712415D1 (de) Verfahren zur amplifikation von nukleinsäure
DE3673171D1 (de) Loesliches, vernetzbares acrylatcopolymerisat, verfahren zu seiner herstellung und ueberzugsmittel auf der basis des acrylatcopolymerisats.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties