ATE366822T1 - Verfahren zum quantitativen nachweis von nukleinsäuren - Google Patents

Verfahren zum quantitativen nachweis von nukleinsäuren

Info

Publication number
ATE366822T1
ATE366822T1 AT99956010T AT99956010T ATE366822T1 AT E366822 T1 ATE366822 T1 AT E366822T1 AT 99956010 T AT99956010 T AT 99956010T AT 99956010 T AT99956010 T AT 99956010T AT E366822 T1 ATE366822 T1 AT E366822T1
Authority
AT
Austria
Prior art keywords
nucleic acids
quantitative detection
calibrator
probes
nucleic acid
Prior art date
Application number
AT99956010T
Other languages
English (en)
Inventor
Giuseppe Locatelli
Paolo Lusso
Mauro Malnati
Francesca Salvatori
Gabriella Scarlatti
Original Assignee
Paolo Lusso
Mauro Malnati
Gabriella Scarlatti
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Paolo Lusso, Mauro Malnati, Gabriella Scarlatti filed Critical Paolo Lusso
Application granted granted Critical
Publication of ATE366822T1 publication Critical patent/ATE366822T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • AIDS & HIV (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT99956010T 1998-11-17 1999-11-17 Verfahren zum quantitativen nachweis von nukleinsäuren ATE366822T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT1998MI002491A IT1303767B1 (it) 1998-11-17 1998-11-17 Metodo di quantificazione di acidi nucleici.

Publications (1)

Publication Number Publication Date
ATE366822T1 true ATE366822T1 (de) 2007-08-15

Family

ID=11381075

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99956010T ATE366822T1 (de) 1998-11-17 1999-11-17 Verfahren zum quantitativen nachweis von nukleinsäuren

Country Status (9)

Country Link
US (2) US7029843B1 (de)
EP (1) EP1131466B1 (de)
JP (1) JP2002530090A (de)
AT (1) ATE366822T1 (de)
AU (1) AU1272000A (de)
CA (1) CA2351740A1 (de)
DE (1) DE69936532D1 (de)
IT (1) IT1303767B1 (de)
WO (1) WO2000029613A1 (de)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8012719B2 (en) * 1998-11-17 2011-09-06 Paolo Lusso Method for the quantitative detection of nucleic acids
JP2002125687A (ja) * 2000-10-30 2002-05-08 Tosoh Corp Ｈｉｖ−１検出のためのオリゴヌクレオチドおよび検出法
AU2002237982A1 (en) * 2001-01-30 2002-08-12 Vertex Pharmaceuticals Incorporated A quantitative assay for nucleic acids
EP1236804A1 (de) 2001-03-02 2002-09-04 Boehringer Mannheim Gmbh Verfahren zur Bestimmung von Nukleinsäuren mittels einer Kontrolle
EP1236805B1 (de) * 2001-03-02 2008-05-07 Roche Diagnostics GmbH Verfahren zur Bestimmung von Nukleinsäuren mittels einer Kontrolle
AU2003213657A1 (en) * 2002-03-04 2003-09-22 U.S. Army Medical Research And Materiel Command Internal positive control for probe-based nucleic acid molecule assays and methods of making and using therof
US7052848B2 (en) 2002-03-04 2006-05-30 The United States Of America As Represented By The Secretary Of The Army Internal positive control for probe-based nucleic acid molecule assays and methods of making and using thereof
EP2163651A1 (de) * 2002-07-26 2010-03-17 Abbott Laboratories Verfahren zum Nachweis und zur Quantifizierung von Hepatitis-C-Virus
US7396646B2 (en) * 2003-01-22 2008-07-08 Modular Genetics, Inc. Alien sequences
WO2004104229A2 (en) * 2003-05-16 2004-12-02 Vickery Michael C L Internal control nucleic acid molecule for nucleic acid amplification systems
CN102329880B (zh) * 2004-04-26 2014-05-14 和光纯药工业株式会社 结核菌检测用的引物和探针以及使用它们检测人型结核菌的方法
US20060110724A1 (en) 2004-11-19 2006-05-25 Burkhardt William Iii Quantitative real-time assay for noroviruses and enteroviruses with built in quality control standard
JP2008182920A (ja) * 2007-01-29 2008-08-14 Sony Corp 蛍光標識オリゴヌクレオチドと該オリゴヌクレオチドを利用する方法
US20080274458A1 (en) * 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
US8366652B2 (en) 2007-08-17 2013-02-05 The Invention Science Fund I, Llc Systems, devices, and methods including infection-fighting and monitoring shunts
JP5223267B2 (ja) * 2007-08-23 2013-06-26 東レ株式会社 遺伝子増幅産物の回収方法および遺伝子増幅産物の回収キット
EP2141246A1 (de) * 2008-07-01 2010-01-06 Koninklijke Philips Electronics N.V. Spaltungslose Verfahren zur PCR-Erkennung mithilfe von SERRS
US9474831B2 (en) 2008-12-04 2016-10-25 Gearbox, Llc Systems, devices, and methods including implantable devices with anti-microbial properties
WO2010065135A1 (en) 2008-12-04 2010-06-10 Searete, Llc System, devices, and methods including actively-controllable sterilizing excitation delivery implants
JP2012531908A (ja) * 2009-07-03 2012-12-13 エイジェンシー フォー サイエンス，テクノロジー アンド リサーチ 結核菌を検出するための方法および／またはプライマー
US8877464B2 (en) 2010-07-29 2014-11-04 Roche Molecular Systems, Inc. Qualitative and quantitative detection of microbial nucleic acids
CN102154515A (zh) * 2011-03-18 2011-08-17 武汉大学 人类疱疹病毒6型实时荧光定量pcr试剂盒
EP2707496A1 (de) 2011-05-11 2014-03-19 Diagon Kft. Verfahren zur schnellen bestimmung von viren mittels nukleinsäurebasierter molekulardiagnostik und kit hierfür
US9101678B2 (en) 2011-11-03 2015-08-11 Elwha Llc Heat-sanitization of surfaces
WO2014013954A1 (ja) * 2012-07-16 2014-01-23 株式会社ダナフォーム 核酸プローブ、核酸プローブの設計方法、およびターゲット配列の検出方法
EA032482B1 (ru) 2012-08-30 2019-06-28 КАБУСИКИ КАЙСЯ ДиЭнЭйФОРМ Способ анализа нуклеиновой кислоты-мишени

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5457027A (en) * 1993-05-05 1995-10-10 Becton, Dickinson And Company Internal controls for isothermal nucleic acid amplification reactions
JPH10505224A (ja) 1994-06-10 1998-05-26 ジョージタウン、ユニバーシティー 組換えウイルスおよびその関連定量法
US6395470B2 (en) * 1997-10-31 2002-05-28 Cenetron Diagnostics, Llc Method for monitoring nucleic acid assays using synthetic internal controls with reversed nucleotide sequences

Also Published As

Publication number Publication date
CA2351740A1 (en) 2000-05-25
US20050239128A1 (en) 2005-10-27
JP2002530090A (ja) 2002-09-17
US7384769B2 (en) 2008-06-10
EP1131466B1 (de) 2007-07-11
AU1272000A (en) 2000-06-05
ITMI982491A1 (it) 2000-05-17
EP1131466A1 (de) 2001-09-12
WO2000029613A1 (en) 2000-05-25
US7029843B1 (en) 2006-04-18
IT1303767B1 (it) 2001-02-23
DE69936532D1 (de) 2007-08-23

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