ATE367440T1 - Schnelles und einfaches verfahren zur isolierung von zirkulären nukleinsäuren - Google Patents
Schnelles und einfaches verfahren zur isolierung von zirkulären nukleinsäurenInfo
- Publication number
- ATE367440T1 ATE367440T1 AT99938037T AT99938037T ATE367440T1 AT E367440 T1 ATE367440 T1 AT E367440T1 AT 99938037 T AT99938037 T AT 99938037T AT 99938037 T AT99938037 T AT 99938037T AT E367440 T1 ATE367440 T1 AT E367440T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acids
- circular nucleic
- isolation
- quick
- easy process
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 4
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 238000002955 isolation Methods 0.000 title 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 230000003196 chaotropic effect Effects 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- 239000000377 silicon dioxide Substances 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/11—Compounds covalently bound to a solid support
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP98109593A EP0969090A1 (de) | 1998-05-27 | 1998-05-27 | Schnelles und einfaches Verfahren zur Isolierung von zirkulären Nucleinsäuren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE367440T1 true ATE367440T1 (de) | 2007-08-15 |
Family
ID=8232012
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99938037T ATE367440T1 (de) | 1998-05-27 | 1999-05-27 | Schnelles und einfaches verfahren zur isolierung von zirkulären nukleinsäuren |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6821757B2 (de) |
| EP (2) | EP0969090A1 (de) |
| JP (1) | JP4369619B2 (de) |
| AT (1) | ATE367440T1 (de) |
| DE (1) | DE69936584T2 (de) |
| WO (1) | WO1999061603A1 (de) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2346615B (en) * | 1998-11-17 | 2003-10-15 | Cambridge Molecular Tech | Isolating nucleic acid |
| JP3752417B2 (ja) | 2000-09-07 | 2006-03-08 | 株式会社日立製作所 | 核酸の精製方法および精製装置 |
| WO2003046146A2 (en) | 2001-11-28 | 2003-06-05 | Applera Corporation | Compositions and methods of selective nucleic acid isolation |
| US20050118647A1 (en) * | 2001-12-31 | 2005-06-02 | Yung-Chiang Chung | Microfluidic mixer apparatus and microfluidic reactor apparatus for microfluidic processing |
| GB0207975D0 (en) * | 2002-04-05 | 2002-05-15 | Genovision As | Isolating nucleic acid |
| AU2002952858A0 (en) * | 2002-11-20 | 2002-12-05 | Bio-Molecular Holdings Pty Limited | Dna isolation material and method |
| US7601491B2 (en) | 2003-02-06 | 2009-10-13 | Becton, Dickinson And Company | Pretreatment method for extraction of nucleic acid from biological samples and kits therefor |
| US20040157219A1 (en) * | 2003-02-06 | 2004-08-12 | Jianrong Lou | Chemical treatment of biological samples for nucleic acid extraction and kits therefor |
| US8377715B2 (en) * | 2003-07-14 | 2013-02-19 | Phynexus, Inc. | Method and device for sample preparation |
| GB0319671D0 (en) * | 2003-08-21 | 2003-09-24 | Secr Defence | Apparatus for processing a fluid sample |
| EP1529840A1 (de) * | 2003-11-04 | 2005-05-11 | Qiagen GmbH | Ein schnelles und preiswertes Verfahren zur Gewinnung von Nukleinsäuren |
| EP1719816B1 (de) * | 2004-02-12 | 2011-06-01 | GL Sciences Incorporated | Mechanismus zur trennung und aufreinigung von dna |
| AU2005200670B2 (en) * | 2004-02-20 | 2007-05-03 | F. Hoffmann-La Roche Ag | Adsorption of nucleic acids to a solid phase |
| KR100486179B1 (ko) * | 2004-03-02 | 2005-05-03 | 피경태 | 핵산을 분리하고 정제하기 위한 세포 용해 조성물, 방법 및 키트 |
| DE102004034433A1 (de) * | 2004-07-15 | 2006-02-02 | Qiagen Gmbh | Verfahren zur Reinigung und Isolierung von Nukleinsäuren unter Verwendung kationischer Detergentien |
| US20060166223A1 (en) * | 2005-01-26 | 2006-07-27 | Reed Michael W | DNA purification and analysis on nanoengineered surfaces |
| JP2007006799A (ja) * | 2005-06-30 | 2007-01-18 | Tokyo Institute Of Technology | プラスミドｄｎａの精製方法及びプラスミドｄｎａ精製用キット |
| KR101066179B1 (ko) * | 2006-02-15 | 2011-09-21 | 토소가부시키가이샤 | 생물재료로부터의 핵산 추출법 |
| US8278118B2 (en) * | 2006-05-26 | 2012-10-02 | Prospect Biosystems, Inc. | Method for the fractionation and separation of particles by step-wise gradient density extraction |
| US8318507B2 (en) * | 2006-05-26 | 2012-11-27 | Marcus Joseph Horn | Method for the fractionation and separation of particles by step-wise gradient density extraction |
| WO2008002882A2 (en) * | 2006-06-26 | 2008-01-03 | Blood Cell Storage, Inc. | Device and method for extraction and analysis of nucleic acids from biological samples |
| US7858363B2 (en) * | 2006-07-22 | 2010-12-28 | Zymo Research Corporation | Plasmid DNA isolation |
| DE102007006008B4 (de) | 2007-02-07 | 2011-08-25 | aj Innuscreen GmbH, 13125 | Verfahren und Kit zur Isolierung von Plasmid DNA |
| DE102007016707A1 (de) * | 2007-04-04 | 2008-10-09 | Qiagen Gmbh | Verfahren zur Aufreinigung von Biomolekülen |
| CA2629589C (en) | 2007-04-20 | 2016-03-29 | F.Hoffmann-La Roche Ag | Isolation and purification of nucleic acid molecules with a solid phase |
| US9428746B2 (en) * | 2007-10-31 | 2016-08-30 | Akonni Biosystems, Inc. | Method and kit for purifying nucleic acids |
| WO2009117167A1 (en) * | 2008-01-02 | 2009-09-24 | Blood Cell Storage, Inc. | Devices and processes for nucleic acid extraction |
| EP2128169A1 (de) * | 2008-05-30 | 2009-12-02 | Qiagen GmbH | Verfahren zur Isolierung von kurzkettigen Nukleinsäuren |
| US8158405B2 (en) * | 2008-06-30 | 2012-04-17 | General Electric Company | Process for concentrating and processing fluid samples |
| US8546127B2 (en) * | 2008-06-30 | 2013-10-01 | General Electric Company | Bacteria/RNA extraction device |
| KR20110101143A (ko) * | 2008-11-04 | 2011-09-15 | 블러드 셀 스토리지 인코퍼레이티드 | 굴곡 유리 표면 상에서의 핵산 추출 |
| KR101569832B1 (ko) * | 2008-11-19 | 2015-11-18 | 삼성전자주식회사 | 시료 중의 게놈 ｄｎａ 및 플라스미드 ｄｎａ를 서로 분리하는 방법 및 그를 위한 키트 |
| EP2598660B1 (de) * | 2010-07-26 | 2017-03-15 | Biomatrica, INC. | Zusammensetzungen zur stabilisierung von dna, rna und proteinen in blut- und anderen biologischen proben während des transports und der lagerung bei umgebungstemperaturen |
| US8816064B2 (en) | 2010-09-30 | 2014-08-26 | Phynexus, Inc. | Purification of nucleic acids |
| US8629264B2 (en) | 2011-05-19 | 2014-01-14 | Blood Cell Storage, Inc. | Gravity flow fluidic device for nucleic acid extraction |
| CN103571824B (zh) | 2012-08-09 | 2016-04-13 | 财团法人工业技术研究院 | 用于分离核酸的组合物及方法 |
| EP3105348B1 (de) | 2014-02-10 | 2019-08-21 | Zymo Research Corporation | Verfahren zur nukleinsäureerfassung |
| SG11201608575QA (en) * | 2014-04-18 | 2016-11-29 | Toppan Printing Co Ltd | Method for recovering short-chain nucleic acids |
| ES2786373T3 (es) | 2014-06-10 | 2020-10-09 | Biomatrica Inc | Estabilización de trombocitos a temperaturas ambiente |
| CN104630208B (zh) * | 2015-02-11 | 2018-01-02 | 杭州百迈生物股份有限公司 | 提取基因组dna的试剂盒和提取方法 |
| KR20250047404A (ko) | 2015-12-08 | 2025-04-03 | 바이오매트리카 인코포레이티드 | 적혈구 침강 속도의 감소 |
| WO2022020483A1 (en) * | 2020-07-22 | 2022-01-27 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for rapid separation and purification of dna topological forms |
| CN113652417B (zh) * | 2021-08-12 | 2022-05-27 | 深圳市卫光生物制品股份有限公司 | 一种质粒dna原液制备方法 |
| CN116393105B (zh) * | 2023-04-21 | 2024-12-03 | 江南大学 | 一种壳聚糖改性凹凸棒土的核酸提取材料的制备及应用 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6027750A (en) * | 1986-09-04 | 2000-02-22 | Gautsch; James | Systems and methods for the rapid isolation of nucleic acids |
| US5075430A (en) * | 1988-12-12 | 1991-12-24 | Bio-Rad Laboratories, Inc. | Process for the purification of DNA on diatomaceous earth |
| US5234809A (en) * | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| DE4321904B4 (de) * | 1993-07-01 | 2013-05-16 | Qiagen Gmbh | Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen |
| US6180778B1 (en) * | 1994-02-11 | 2001-01-30 | Qiagen Gmbh | Process for the separation of double-stranded/single-stranded nucleic acid structures |
| GB9602825D0 (en) * | 1996-02-12 | 1996-04-10 | Therexsys Ltd | Method of plasmid dna production and purification |
| US6027945A (en) * | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
-
1998
- 1998-05-27 EP EP98109593A patent/EP0969090A1/de not_active Withdrawn
-
1999
- 1999-05-27 AT AT99938037T patent/ATE367440T1/de not_active IP Right Cessation
- 1999-05-27 EP EP99938037A patent/EP1088064B1/de not_active Expired - Lifetime
- 1999-05-27 US US09/341,119 patent/US6821757B2/en not_active Expired - Lifetime
- 1999-05-27 DE DE69936584T patent/DE69936584T2/de not_active Expired - Lifetime
- 1999-05-27 WO PCT/EP1999/003660 patent/WO1999061603A1/en not_active Ceased
- 1999-05-27 JP JP2000550988A patent/JP4369619B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO1999061603A1 (en) | 1999-12-02 |
| DE69936584T2 (de) | 2008-03-06 |
| US20030032147A1 (en) | 2003-02-13 |
| EP1088064B1 (de) | 2007-07-18 |
| EP1088064A1 (de) | 2001-04-04 |
| DE69936584D1 (de) | 2007-08-30 |
| US6821757B2 (en) | 2004-11-23 |
| JP4369619B2 (ja) | 2009-11-25 |
| EP0969090A1 (de) | 2000-01-05 |
| JP2002516094A (ja) | 2002-06-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE367440T1 (de) | Schnelles und einfaches verfahren zur isolierung von zirkulären nukleinsäuren | |
| DE59912604D1 (de) | Verfahren zur isolierung und aufreinigung von nucleinsäuren | |
| ATE144777T1 (de) | Brett-stabiles produkt und verfahren zur isolierung der rna, dna und proteine | |
| ATE156830T1 (de) | Verfahren zur reinigung von nukleinsäuren | |
| DE59209997D1 (de) | Verfahren zur Isolierung und Reinigung von Nukleinsäuren | |
| ATE91708T1 (de) | Verfahren zur freisetzung von rns und dns aus zellen. | |
| ATE311474T1 (de) | Verfahren zur amplifikation und zur sequenzierung von nukleinsäuren | |
| DE69331665D1 (de) | Methode zur behandlung von nukleinsäureproben | |
| ATE215611T1 (de) | Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien | |
| DE69837041D1 (de) | Verfahren und mikroorganism zur herstellung von purin-nukleosiden durch fermentation | |
| EP0557871A3 (en) | Method and apparatus for automated assay of biological specimens | |
| DE69315342D1 (de) | Vorrichtung und verfahren zur reinigung von nukleinsauren | |
| DE69832597D1 (de) | Verfahren zur verarbeitung von kakaomasse | |
| EP0777750A4 (de) | Verfahren mit einer hohen umsatzrate für das auffinden von sequenzen oder genetischen veränderungen in nukleinsäuren | |
| DE69316205D1 (de) | Verfahren und reagens zur spaltung und entschützen von oligonukleotide | |
| DE60012549D1 (de) | Verfahren zur isolierung von nukleinsäuren und kit | |
| DE69710551D1 (de) | Verfahren zur Entfernung von Stickstoff und immobilisierte Mikroorganismen | |
| DE69637579D1 (de) | Methode zur Amplifizierung von Nukleinsäure und entsprechende Regenzien dafür | |
| DE69312455D1 (de) | Verfahren und Vorrichtung zur Entsorgung von organischen Abfallstoffen | |
| DE59005821D1 (de) | Verfahren zur Herstellung von L-Isoleucin und dafür geeignete Mikroorganismen und rekombinante DNA. | |
| DE59712415D1 (de) | Verfahren zur amplifikation von nukleinsäure | |
| DE69832662D1 (de) | Verfahren zur Kultur von Hepatocyten | |
| DE69817000D1 (de) | Verfahren zur schnellen isolierung von rns | |
| ATA79991A (de) | Verfahren zum, insbesondere beschleunigten, aeroben mikrobiologischen abbau von biologischen substanzen | |
| ATE396279T1 (de) | Verfahren zur isolierung, amplifizierung und charakterisierung von dns |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |