ATE144777T1 - Brett-stabiles produkt und verfahren zur isolierung der rna, dna und proteine - Google Patents

Brett-stabiles produkt und verfahren zur isolierung der rna, dna und proteine

Info

Publication number
ATE144777T1
ATE144777T1 AT93300522T AT93300522T ATE144777T1 AT E144777 T1 ATE144777 T1 AT E144777T1 AT 93300522 T AT93300522 T AT 93300522T AT 93300522 T AT93300522 T AT 93300522T AT E144777 T1 ATE144777 T1 AT E144777T1
Authority
AT
Austria
Prior art keywords
proteins
dna
rna
brett
isolation
Prior art date
Application number
AT93300522T
Other languages
English (en)
Inventor
Piotr Chomczynski
Original Assignee
Piotr Chomczynski
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Piotr Chomczynski filed Critical Piotr Chomczynski
Application granted granted Critical
Publication of ATE144777T1 publication Critical patent/ATE144777T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT93300522T 1992-01-28 1993-01-25 Brett-stabiles produkt und verfahren zur isolierung der rna, dna und proteine ATE144777T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/826,984 US5346994A (en) 1992-01-28 1992-01-28 Shelf-stable product and process for isolating RNA, DNA and proteins

Publications (1)

Publication Number Publication Date
ATE144777T1 true ATE144777T1 (de) 1996-11-15

Family

ID=25248018

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93300522T ATE144777T1 (de) 1992-01-28 1993-01-25 Brett-stabiles produkt und verfahren zur isolierung der rna, dna und proteine

Country Status (6)

Country Link
US (1) US5346994A (de)
EP (1) EP0554034B1 (de)
JP (1) JP2679929B2 (de)
AT (1) ATE144777T1 (de)
DE (1) DE69305662T2 (de)
ES (1) ES2095565T3 (de)

Families Citing this family (131)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5637687A (en) * 1993-08-31 1997-06-10 Wiggins; James C. Methods and compositions for isolating nucleic acids
WO1995028409A1 (en) * 1994-04-14 1995-10-26 The Rockefeller University Process, apparatus and reagents for isolating cellular components
US5693784A (en) * 1994-09-19 1997-12-02 Promega Corporation Methods for creating agglomerates from colloidal particles
US5777099A (en) * 1995-02-24 1998-07-07 Biotecx Laboratories, Inc. RNA separation
DE19520398B4 (de) * 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
KR100463475B1 (ko) 1995-06-08 2005-06-22 로셰 디아그노스틱스 게엠베하 자기성피그먼트
US5945515A (en) * 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
US5804684A (en) * 1995-08-24 1998-09-08 The Theobald Smith Research Institute, Inc. Method for isolating nucleic acids
US5973137A (en) * 1996-02-13 1999-10-26 Gentra Systems, Inc. Low pH RNA isolation reagents, method, and kit
US5777098A (en) * 1996-07-23 1998-07-07 University Of North Dakota Medical Education Research Foundation DNA purification procedure
SE9603599D0 (sv) * 1996-10-02 1996-10-02 Pharmacia Biotech Ab A method of separating a two phase system
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
US20050287583A1 (en) * 1997-01-21 2005-12-29 Promega Corporation Methods and kits for isolating biological target materials using silica magnetic particles
JP2001524820A (ja) * 1997-04-10 2001-12-04 ライフ テクノロジーズ,インコーポレイテッド Rna単離試薬および方法
DE19743518A1 (de) * 1997-10-01 1999-04-15 Roche Diagnostics Gmbh Automatisierbare universell anwendbare Probenvorbereitungsmethode
US20050042597A1 (en) * 1997-10-07 2005-02-24 Pham Thuy Diem Viral detection system
WO1999029904A2 (en) * 1997-12-10 1999-06-17 Sierra Diagnostics, Inc. Methods and reagents for preservation of dna in bodily fluids
US6194562B1 (en) 1998-04-22 2001-02-27 Promega Corporation Endotoxin reduction in nucleic acid purification
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
US6204375B1 (en) 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
DE19836559A1 (de) 1998-08-12 2000-03-23 Antigen Gmbh Gefäß zur Entnahme von Blut
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US7264932B2 (en) * 1999-09-24 2007-09-04 Applera Corporation Nuclease inhibitor cocktail
DE60044977D1 (de) * 1999-09-24 2010-10-28 Ambion Inc Cocktail von nukleaseinhibitoren
WO2001037291A1 (en) * 1999-11-17 2001-05-25 Roche Diagnostics Gmbh Magnetic glass particles, method for their preparation and uses thereof
US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
DE10006662A1 (de) * 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
US6602718B1 (en) 2000-11-08 2003-08-05 Becton, Dickinson And Company Method and device for collecting and stabilizing a biological sample
US20020142328A1 (en) * 2000-12-01 2002-10-03 Danenberg Kathleen D. Method of determining a chemotherapeutic regimen by assaying gene expression in primary tumors
US6582919B2 (en) 2001-06-11 2003-06-24 Response Genetics, Inc. Method of determining epidermal growth factor receptor and HER2-neu gene expression and correlation of levels thereof with survival rates
US6602670B2 (en) * 2000-12-01 2003-08-05 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 expression
US7005278B2 (en) * 2001-03-02 2006-02-28 Danenberg Kathleen D Method of determining dihydropyrimidine dehydrogenase gene expression
US7049059B2 (en) * 2000-12-01 2006-05-23 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 and TS expression
NZ546252A (en) * 2000-12-01 2008-03-28 Response Genetics Inc Method of determining HER2-neu gene expression in paraffin embedded tissue samples
ATE324376T1 (de) * 2001-01-16 2006-05-15 Invitrogen Corp Reagenz zur isolierung von rna
US6956111B2 (en) * 2001-03-02 2005-10-18 Response Genetics, Inc. Method of determining dihydropyrimidine dehydrogenase gene expression
AU2002252370A1 (en) * 2001-03-12 2002-09-24 Irm, Llc. Genomics-driven high speed cellular assays, development thereof, and collections of cellular reporters
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7235358B2 (en) * 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6686155B2 (en) * 2001-06-14 2004-02-03 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on glutathione-S-transferase pi expression
US7893228B2 (en) * 2001-10-12 2011-02-22 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
US20050032105A1 (en) * 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
US7148343B2 (en) * 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
JP2005507997A (ja) * 2001-10-26 2005-03-24 イムニベスト・コーポレイション 同一試料についての包括的核酸および形態学的特徴のマルチパラメーター分析
WO2003046146A2 (en) 2001-11-28 2003-06-05 Applera Corporation Compositions and methods of selective nucleic acid isolation
US8025873B2 (en) 2002-06-20 2011-09-27 Paladin Labs, Inc. Chimeric antigens for eliciting an immune response
US8029803B2 (en) * 2002-06-20 2011-10-04 Paladin Labs, Inc. Chimeric antigens for eliciting an immune response
WO2004076640A2 (en) * 2003-02-25 2004-09-10 Ambion, Inc. Small-molecule inhibitors of angiogenin and rnases and in vivo and in vitro methods of using same
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7267950B2 (en) * 2003-05-01 2007-09-11 Veridex, Lcc Rapid extraction of RNA from cells and tissues
US7488579B2 (en) * 2003-05-06 2009-02-10 Biochain Institute, Inc. Methods and compositions to extract DNA, RNA and protein simultaneously from biological samples
WO2004108925A1 (en) * 2003-06-04 2004-12-16 Qiagen As Method for sequentially isolating dna and rna from the same nucleic acid-containing sample
WO2005010204A2 (en) * 2003-07-21 2005-02-03 Creighton University Rapid nucleic acid isolation method and compositions therefore
US20050042660A1 (en) * 2003-07-31 2005-02-24 Hall Gerald Edward Devices and methods for isolating RNA
US8007805B2 (en) * 2003-08-08 2011-08-30 Paladin Labs, Inc. Chimeric antigens for breaking host tolerance to foreign antigens
WO2005023091A2 (en) 2003-09-05 2005-03-17 The Trustees Of Boston University Method for non-invasive prenatal diagnosis
EP1689884A4 (de) 2003-10-08 2007-04-04 Univ Boston Verfahren zur pränatalen diagnose chromosomaler abnormalitäten
DE602004026912D1 (de) * 2003-12-16 2010-06-10 Qiagen North American Holdings Formulierungen und verfahren zum denaturieren von proteinen
US20050164204A1 (en) * 2004-01-27 2005-07-28 Reed Thomas D. Single use lyophilized rnase reagents, and kits and methods for using same
EP1584923A3 (de) * 2004-04-07 2006-01-04 Roche Diagnostics GmbH Stabilisierung von Biomolekülen in Proben
US7794932B2 (en) * 2004-04-16 2010-09-14 Piotr Chomczynski Reagents and methods for isolation of purified RNA
US7531308B2 (en) * 2004-04-23 2009-05-12 Sigma-Aldrich Co. Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography
US20050277121A1 (en) * 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
US20060099605A1 (en) * 2004-11-11 2006-05-11 Hall Gerald E Jr Devices and methods for isolating RNA
WO2006029184A2 (en) 2004-09-08 2006-03-16 Expression Diagnostics, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
US20060105372A1 (en) 2004-11-05 2006-05-18 Bair Robert J Compositions and methods for purifying nucleic acids from stabilization reagents
DK1817423T3 (en) 2004-11-30 2015-01-26 Merial Ltd Mixing units for chemical light ring of cells
US7727718B2 (en) * 2005-01-04 2010-06-01 Molecular Research Center, Inc. Reagents for storage and preparation of samples for DNA analysis
CN101268198A (zh) * 2005-01-21 2008-09-17 纽约血液中心有限公司 用于核酸提取和鉴定的方法
US7541144B2 (en) * 2005-01-31 2009-06-02 Agilent Technologies, Inc. RNA labeling method
DE102005015005A1 (de) * 2005-04-01 2006-10-05 Qiagen Gmbh Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe
US20060270843A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods for isolation of nucleic acids
US20060269929A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods and kits for DNA purification on polymeric membranes at low ionic strength
US20070015191A1 (en) * 2005-07-01 2007-01-18 Promega Corporation Network of buoyant particles for biomolecule purification and use of buoyant particles or network of buoyant particles for biomolecule purification
US7544471B2 (en) * 2005-07-30 2009-06-09 Agilent Technologies, Inc. Preparing RNA from a wax-embedded tissue specimen
US20070054168A1 (en) * 2005-09-06 2007-03-08 Hao Chang Zinc/air cell
CA2623329A1 (en) * 2005-10-13 2007-04-19 Virexx Medical Corp. Chimeric antigen containing hepatitis c virus polypeptide and fc fragment for eliciting an immune response
EP1963526A4 (de) 2005-12-09 2009-11-18 Promega Corp Nukleinsäurereinigung mittels bindungsmatrix
CA2651815A1 (en) * 2006-05-10 2007-11-22 Dxterity Diagnostics Detection of nucleic acid targets using chemically reactive oligonucleotide probes
EP3617321B1 (de) 2006-05-31 2024-10-23 Sequenom, Inc. Kit zur nukleinsäureextraktion und -verstärkung aus einer probe
US20070292882A1 (en) * 2006-06-16 2007-12-20 Sysmex Corporation Method and apparatus for detecting cancer cell
JP2008289456A (ja) * 2006-06-16 2008-12-04 Sysmex Corp がん細胞の検出方法及び装置
US20080003585A1 (en) * 2006-06-29 2008-01-03 Bio-Rad Laboratories, Inc., A Corporation Of The State Of Delaware Purification and amplification of nucleic acids in a microfluidic device
US7687254B2 (en) * 2006-07-11 2010-03-30 Case Western Reserve University Phenol-free method of isolating DNA
AU2007284651B2 (en) 2006-08-09 2014-03-20 Institute For Systems Biology Organ-specific proteins and methods of their use
WO2008021431A2 (en) 2006-08-14 2008-02-21 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
EP2102367A2 (de) 2006-11-09 2009-09-23 XDX, Inc. Verfahren zur diagnose und überwachung des status von systemischem lupus erythematodes
WO2008150187A1 (fr) * 2007-06-08 2008-12-11 Jury Fedorovich Drygin Utilisation du trichloracétate d'ammonium en tant que produit de dissociation de complexes naturels d'acides nucléiques et procédé d'extraction d'arn
EP2214824A4 (de) * 2007-11-28 2015-08-19 Smart Tube Inc Vorrichtungen, systeme und verfahren zur entnahme, stimulation, stabilisierung und analyse einer biologischen probe
WO2009127350A1 (en) * 2008-04-15 2009-10-22 Universität Bern Method for optimized isolation of rna from fixed tissue
US20110111417A1 (en) 2008-05-14 2011-05-12 Millennium Pharmaceuticals, Inc. Methods and kits for monitoring the effects of immunomodulators on adaptive immunity
DE102008029356A1 (de) 2008-06-20 2009-12-24 Siemens Healthcare Diagnostics Gmbh Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe
DE102008061714A1 (de) 2008-12-12 2010-06-17 Siemens Healthcare Diagnostics Inc., Deerfield Verfahren zur Aufreinigung von Nukleinsäuren, inbesondere aus fixiertem Gewebe
WO2010070657A1 (en) * 2008-12-15 2010-06-24 Council Of Scientific & Industrial Research A composition for the removal of colours and inhibitors from plant tissues to isolate rna
US8846878B1 (en) 2009-01-23 2014-09-30 Cubrc Corporation Method and device for isolating a protein sample
CA2757300C (en) * 2009-04-01 2018-01-09 Dxterity Diagnostics Incorporated Chemical ligation dependent probe amplification (clpa)
EP3514244B1 (de) 2009-04-03 2021-07-07 Sequenom, Inc. Verfahren für die herstellung von nukleinsäuren
PL2443226T3 (pl) * 2009-06-18 2015-04-30 Merck Patent Gmbh Sposób izolowania komórek
DE202009018981U1 (de) * 2009-07-01 2015-03-11 Axagarius Gmbh & Co. Kg Verwendung eines Kits für die Isolierung von Biomolekülen aus biologischen Proben sowie Kit dafür
US8222397B2 (en) * 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
WO2011026030A1 (en) * 2009-08-31 2011-03-03 Mbio Diagnostics Corporation Integrated sample preparation and analyte detection
KR101641113B1 (ko) 2010-08-18 2016-07-20 도레이 카부시키가이샤 Rna 추출용 용액
EP2426201A1 (de) 2010-09-06 2012-03-07 Qiagen GmbH Verfahren zur Isolierung von gereinigter RNS mit verminderter DNS Kontamination
US9051563B2 (en) 2011-01-14 2015-06-09 Zymo Research Corporation Nucleic acid purification
US9745616B2 (en) 2011-05-17 2017-08-29 Dxterity Diagnostics Incorporated Methods and compositions for detecting target nucleic acids
EP3789500A1 (de) 2011-08-12 2021-03-10 QIAGEN GmbH Verfahren zur isolierung von nukleinsäuren
CN103890176B (zh) 2011-09-06 2016-10-26 西北大学 制备生物材料的方法
JP6238900B2 (ja) 2011-10-28 2017-11-29 ミレニアム ファーマシューティカルズ, インコーポレイテッドMillennium Pharmaceuticals, Inc. Nae阻害物質に対する応答のバイオマーカー
CN102505012A (zh) * 2011-12-30 2012-06-20 珠海出入境检验检疫局检验检疫技术中心 一种用于提取动物病毒rna的裂解液
WO2013169861A1 (en) 2012-05-09 2013-11-14 Bio-Rad Laboratories, Inc. Buffer for one-step dna extraction
US9206469B2 (en) 2012-07-18 2015-12-08 Zymo Research Corporation Nucleic acid purification
EP2904115B1 (de) 2012-10-01 2018-08-08 Millennium Pharmaceuticals, Inc. Biomarker und verfahren zur vorhersage der reaktion auf inhibitoren und verwendung davon
JP6392857B2 (ja) 2013-05-24 2018-09-19 オッカム バイオラブス,インコーポレイティド 核酸サンプルを回収するためのシステム及び方法
UY36046A (es) 2014-03-26 2015-10-30 Millennium Pharm Inc Formulaciones farmacéuticas, procesos para la preparación y métodos de uso
CN106879252B (zh) 2014-06-10 2020-07-17 德克斯特里蒂诊断公司 用于收集和稳定生物样品的装置及方法
US10093989B2 (en) 2014-10-20 2018-10-09 Gen-Probe Incorporated Red blood cell lysis solution
RU2729113C2 (ru) 2014-11-21 2020-08-04 Оккам Байолэбс, Инк. Система и способ сбора образца нуклеиновой кислоты
EP3135769A1 (de) 2015-08-26 2017-03-01 Qiagen GmbH Kits und verfahren zur extraktion von rna
EP3397763B1 (de) * 2015-12-28 2020-10-28 Koninklijke Philips N.V. Nukleinsäurenreinigungssystem unter verwendung einer einzigen wasch- und eluierungspufferlösung
EP3436580B1 (de) 2016-03-30 2021-03-17 Life Technologies AS Verbesserte verfahren und zusammensetzungen für nukleinsäureisolierung
EP3448998B1 (de) 2016-04-27 2020-06-03 Gen-Probe Incorporated Reagens für blutzelllyse
US20200080074A1 (en) 2016-12-15 2020-03-12 Qiagen Gmbh Method for isolating highly pure nucleic acid with magnetic particles
BR112019019300B1 (pt) 2017-03-17 2023-04-18 Syngenta Participations Ag Método para preservar uma amostra de tecido vegetal contendo ácidos nucleicos para uso em análise de dna, reagente para o processamento de uma amostra biológica contendo ácidos nucleicos, kit para armazenamento de amostras de tecido contendo ácidos nucleicos e métodos para extrair ácidos nucleicos e para a realização de genotipagem (ou qpcr) em ácidos nucleicos preservados
WO2019109016A1 (en) 2017-12-01 2019-06-06 Millennium Pharmaceuticals, Inc. Biomarkers and methods for treatment with nae inhibitors
KR102262992B1 (ko) * 2019-04-15 2021-06-09 대구가톨릭대학교산학협력단 리포폴리사카라이드 추출용 용매 및 이를 이용한 리포폴리사카라이드 추출 방법
CN110951724A (zh) * 2019-12-20 2020-04-03 沈阳农业大学 一种Trizol快速提取核酸DNA和/或RNA的方法
CN112195176B (zh) * 2020-09-30 2023-05-02 天津大潮基因科技有限公司 从生物材料中分离纯化核酸固体的方法
CN117467731A (zh) * 2023-11-07 2024-01-30 合肥贝壳派创新科技有限公司 一种贻贝提取物的制备方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4843155A (en) * 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5010183A (en) * 1989-07-07 1991-04-23 Macfarlane Donald E Process for purifying DNA and RNA using cationic detergents
US5128247A (en) * 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
CA2087105C (en) * 1990-07-13 2000-09-12 Jeffrey Van Ness Non-corrosive composition and methods useful for the extraction of nucleic acids
US5130423A (en) * 1990-07-13 1992-07-14 Microprobe Corporation Non-corrosive compositions and methods useful for the extraction of nucleic acids

Also Published As

Publication number Publication date
JP2679929B2 (ja) 1997-11-19
JPH05344886A (ja) 1993-12-27
DE69305662T2 (de) 1997-02-27
US5346994A (en) 1994-09-13
EP0554034A1 (de) 1993-08-04
ES2095565T3 (es) 1997-02-16
DE69305662D1 (de) 1996-12-05
EP0554034B1 (de) 1996-10-30

Similar Documents

Publication Publication Date Title
ATE144777T1 (de) Brett-stabiles produkt und verfahren zur isolierung der rna, dna und proteine
DE69132098D1 (de) Verfahren zur amplifikation von nukleinsäuren
ATE156830T1 (de) Verfahren zur reinigung von nukleinsäuren
AU600997B2 (en) Method of isolating and purifying nucleic acids from biological samples
EP1961826A3 (de) Verfahren zur direkten Sequenzierung von Nukleinsäuren
ATE193301T1 (de) Verfahren zur isolierung von proteinen aus milch
ATE367440T1 (de) Schnelles und einfaches verfahren zur isolierung von zirkulären nukleinsäuren
DE69331665D1 (de) Methode zur behandlung von nukleinsäureproben
CA2260940A1 (en) Methods for isolating nucleic acids using alkaline protease
DE59409712D1 (de) Verfahren zur chromatographischen reinigung und trennung von nucleinsäuregemischen
DE69632466D1 (de) Lösung die chaotropische mittel enthält und verwendung dieser lösung in verfahren zur isolierung von dna, rna und proteinen
FI901917A7 (fi) Menetelmä nukleiinihapon uuttamiseksi amplifioimiseksi ja havaitsemiseksi kokoverestä tai PMBC-jakeesta
GB9425138D0 (en) Isolation of nucleic acid
DE69435030D1 (de) Methode zur Detektion eines DNA Zielmoleküls
DE69720473D1 (de) Verfahren zur reinigung von nukleinsäuren
DE69929882D1 (de) Verfahren und vorrichtung zur nukleinsäurereinigung
DE69626957D1 (de) Verfahren zur reduzierung der rns-konzentration in einer mischung biologischen materials unter verwendung von diatomeen-erde
ATE76899T1 (de) Vorrichtung und verfahren zur blasenfreien begasung von fluessigkeiten, insbesondere von kulturmedien zur vermehrung von gewebekulturen.
ATE236999T1 (de) Verfahren zur reinigung bzw. isolierung viraler nukleinsäuren
AU2001260714A1 (en) Method of analyzing components in biological samples
DE69938774D1 (de) Verfahren zur isolierung, amplifizierung und charakterisierung von dns
EP0751226A3 (de) Verfahren für die Amplifizierung von Nukleinsäure-Sequenzen
AU5040590A (en) Method for removing dna from protein preparations
DE3874147D1 (de) Verfahren zur teilweisen reinigung eines oxyiodierungsproduktes.
DE3677427D1 (de) Verfahren zur kontinuierlichen abscheidung von maleinsaeureanhydrid aus gasfoermigen reaktionsgemischen.

Legal Events

Date Code Title Description
REN Ceased due to non-payment of the annual fee