ATE367447T1 - Lentivirale verpackungszellen - Google Patents

Lentivirale verpackungszellen

Info

Publication number
ATE367447T1
ATE367447T1 AT99923020T AT99923020T ATE367447T1 AT E367447 T1 ATE367447 T1 AT E367447T1 AT 99923020 T AT99923020 T AT 99923020T AT 99923020 T AT99923020 T AT 99923020T AT E367447 T1 ATE367447 T1 AT E367447T1
Authority
AT
Austria
Prior art keywords
cell lines
gag
pol
proteins
packaging cells
Prior art date
Application number
AT99923020T
Other languages
English (en)
Inventor
Philippe Leboulch
Karen Westerman
Original Assignee
Genetix Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetix Pharmaceuticals Inc filed Critical Genetix Pharmaceuticals Inc
Application granted granted Critical
Publication of ATE367447T1 publication Critical patent/ATE367447T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16051—Methods of production or purification of viral material
    • C12N2740/16052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Packages (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Wrappers (AREA)
  • Polyesters Or Polycarbonates (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT99923020T 1998-05-13 1999-05-13 Lentivirale verpackungszellen ATE367447T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US8528398P 1998-05-13 1998-05-13

Publications (1)

Publication Number Publication Date
ATE367447T1 true ATE367447T1 (de) 2007-08-15

Family

ID=22190591

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99923020T ATE367447T1 (de) 1998-05-13 1999-05-13 Lentivirale verpackungszellen

Country Status (11)

Country Link
US (4) US6365150B1 (de)
EP (1) EP1076715B1 (de)
AT (1) ATE367447T1 (de)
AU (1) AU3988799A (de)
CA (1) CA2328404C (de)
CY (1) CY1107760T1 (de)
DE (1) DE69936577T2 (de)
DK (1) DK1076715T3 (de)
ES (1) ES2291025T3 (de)
PT (1) PT1076715E (de)
WO (1) WO1999058701A1 (de)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020173643A1 (en) * 1995-04-14 2002-11-21 Kappes John C. Fusion protein delivery system and uses thereof
US5994136A (en) * 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
ES2291025T3 (es) * 1998-05-13 2008-02-16 Genetix Pharmaceuticals Inc. Celulas lentivirales de empaquetamiento.
US6555342B1 (en) 1998-06-03 2003-04-29 Uab Research Foundation Fusion protein delivery system and uses thereof
US7622300B2 (en) * 1998-06-03 2009-11-24 Kappes John C Trans-lentiviral vector particles and transduction of eukaryotic cells therewith
ATE368122T1 (de) * 1999-04-29 2007-08-15 Cell Genesys Inc Verfahren und mittel für die herstellung von sicheren rekombinanten lentiviralen vektoren mit hohem titer
EP1103615A1 (de) * 1999-11-25 2001-05-30 Universite De Geneve Vektoren, die nicht-teilende Zellen immortalisieren können und Zellen, die mit diesen Vektoren immortalisiert wurden
US20040248113A1 (en) * 1999-12-28 2004-12-09 Alessandro Sette Method and system for optimizing multi-epitope nucleic acid constructs and peptides encoded thereby
AU2002311777A1 (en) * 2001-04-17 2002-10-28 Genetix Pharmaceuticals, Inc. Method of treating arthritis using lentiviral vectors in gene therapy
US20030039636A1 (en) * 2001-05-01 2003-02-27 Genetix Pharmaceuticals, Inc. Novel self-inactivating (SIN) lentiviral vectors
WO2002100317A2 (en) * 2001-05-25 2002-12-19 The Trustees Of The University Of Pennsylvania Targeted particles and methods of using the same
ATE303169T1 (de) 2001-11-19 2005-09-15 Scil Technology Gmbh Homogen beschichtete vorrichtung mit osteoinduktiven und osteokonduktiven eigenschaften
CN100522241C (zh) * 2002-09-10 2009-08-05 Scil技术股份有限公司 在低氧气浓度下用骨诱导蛋白包被的金属植入物
KR100553154B1 (ko) * 2002-10-21 2006-02-22 유지창 Ｈｉｖ 뉴클레오캡시드 단백질의 동물세포 발현용 벡터 및 이의 제조방법
WO2004113547A2 (en) * 2003-05-19 2004-12-29 The Government Of The United States, As Represented By The Secretary Of Health And Human Services Potent combinations of mrna transport elements
FR2872170B1 (fr) 2004-06-25 2006-11-10 Centre Nat Rech Scient Cnrse Lentivirus non interactif et non replicatif, preparation et utilisations
EP2425001A4 (de) 2009-04-30 2012-11-14 Univ California Anti-hiv-kombinationsvektoren, targeting-vektoren und anwendungsverfahren
WO2011119920A2 (en) 2010-03-25 2011-09-29 Oregon Health & Science University Cmv glycoproteins and recombinant vectors
ES2667425T3 (es) 2011-06-10 2018-05-10 Oregon Health & Science University Glucoproteínas y vectores recombinantes de CMV
WO2014026053A1 (en) 2012-08-09 2014-02-13 The Regents Of The University Of California Cd25 pre-selective combination anti-hiv vectors, targeting vectors, and methods of use
US20140065110A1 (en) 2012-08-31 2014-03-06 The Regents Of The University Of California Genetically modified msc and therapeutic methods
JP6454352B2 (ja) * 2013-11-05 2019-01-16 タカラ バイオ ユーエスエー， インコーポレイテッド 乾燥トランスフェクション組成物、乾燥トランスフェクション組成物を生成する方法、及び乾燥トランスフェクション組成物を使用する方法
CN106794260B (zh) 2014-04-25 2021-02-23 儿童医疗中心有限公司 治疗血红蛋白病的组合物和方法
CN109154005A (zh) 2016-02-23 2019-01-04 免疫设计股份有限公司 多基因组逆转录病毒载体制剂及用于产生及使用该制剂的方法和系统
EP3276006A1 (de) * 2016-07-27 2018-01-31 Theravectys Lentivirale vektoren zur expression von hepatitis-b-virus (hbv)-antigenen
US12350284B2 (en) 2018-05-02 2025-07-08 The Children's Medical Center Corporation BCL11A microRNAs for treating hemoglobinopathies
JP7470119B2 (ja) 2018-08-24 2024-04-17 シーエスエル ベーリング ジーン セラピー インコーポレイテッド 無血清培地におけるベクター産生
WO2020102709A1 (en) * 2018-11-16 2020-05-22 The Regents Of The University Of California Compositions and methods for delivering crispr/cas effector polypeptides
WO2020172259A1 (en) 2019-02-20 2020-08-27 Research Institute At Nationwide Children's Hospital Cancer-targeted, virus-encoded, regulatable t (catvert) or nk cell (catvern) linkers
US11912986B2 (en) 2019-09-23 2024-02-27 The Regents Of The University Of California Methods for screening genetic perturbations
WO2022155500A1 (en) 2021-01-14 2022-07-21 Senti Biosciences, Inc. Secretable payload regulation
CN113186353A (zh) * 2021-05-28 2021-07-30 杭州晶佰生物技术有限公司 针对c型逆转录病毒的数字pcr检测用引物及检测方法
US12565647B2 (en) 2021-05-28 2026-03-03 The Regents Of The University Of California Compositions and methods for targeted delivery of CRISPR-Cas effector polypeptides and transgenes
WO2022261150A2 (en) 2021-06-09 2022-12-15 Scribe Therapeutics Inc. Particle delivery systems
WO2026096338A1 (en) 2024-11-04 2026-05-07 St. Jude Children's Research Hospital, Inc. Rps19-encoding lentiviral vector and method of using the same

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5861161A (en) 1994-09-07 1999-01-19 Universite De Montreal Chimeric proteins comprising a Vpr/Vpx virion incorporation domain for targeting into HIV-1 or HIV-2 virions
ATE408015T1 (de) 1995-04-14 2008-09-15 Univ Alabama Res Found Transfer system basierend auf einem fusionsprotein und seine anwendungen.
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
EP0859856A2 (de) 1995-11-08 1998-08-26 Whitehead Institute For Biomedical Research Stabile verpackungszellinie, die pseudotyp-retroviren produziert
CA2265522A1 (en) * 1996-09-17 1998-03-26 The Salk Institute For Biological Studies Retroviral vectors capable of transducing non-dividing cells
US5908773A (en) 1996-09-20 1999-06-01 Cornell Research Foundation, Inc. KSHV positive cell lines
GB9621679D0 (en) 1996-10-17 1996-12-11 Oxford Biomedica Ltd Improved retroviral vectors
PT904392E (pt) 1996-10-17 2001-06-29 Oxford Biomedica Ltd Vectores retrovirais
ES2291025T3 (es) 1998-05-13 2008-02-16 Genetix Pharmaceuticals Inc. Celulas lentivirales de empaquetamiento.

Also Published As

Publication number Publication date
US6955919B2 (en) 2005-10-18
US7311907B2 (en) 2007-12-25
CA2328404C (en) 2007-07-24
DE69936577D1 (de) 2007-08-30
US20080187997A1 (en) 2008-08-07
DE69936577T2 (de) 2008-04-30
PT1076715E (pt) 2007-10-26
US20020168346A1 (en) 2002-11-14
US20050170507A1 (en) 2005-08-04
CA2328404A1 (en) 1999-11-18
WO1999058701A1 (en) 1999-11-18
US6365150B1 (en) 2002-04-02
EP1076715A1 (de) 2001-02-21
US8034620B2 (en) 2011-10-11
ES2291025T3 (es) 2008-02-16
AU3988799A (en) 1999-11-29
EP1076715B1 (de) 2007-07-18
DK1076715T3 (da) 2007-11-19
CY1107760T1 (el) 2013-06-19

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