ATE367447T1 - Lentivirale verpackungszellen - Google Patents

Lentivirale verpackungszellen

Info

Publication number
ATE367447T1
ATE367447T1 AT99923020T AT99923020T ATE367447T1 AT E367447 T1 ATE367447 T1 AT E367447T1 AT 99923020 T AT99923020 T AT 99923020T AT 99923020 T AT99923020 T AT 99923020T AT E367447 T1 ATE367447 T1 AT E367447T1
Authority
AT
Austria
Prior art keywords
cell lines
gag
pol
proteins
packaging cells
Prior art date
Application number
AT99923020T
Other languages
English (en)
Inventor
Philippe Leboulch
Karen Westerman
Original Assignee
Genetix Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetix Pharmaceuticals Inc filed Critical Genetix Pharmaceuticals Inc
Application granted granted Critical
Publication of ATE367447T1 publication Critical patent/ATE367447T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16051—Methods of production or purification of viral material
    • C12N2740/16052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Packages (AREA)
  • Polyesters Or Polycarbonates (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Wrappers (AREA)
AT99923020T 1998-05-13 1999-05-13 Lentivirale verpackungszellen ATE367447T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US8528398P 1998-05-13 1998-05-13

Publications (1)

Publication Number Publication Date
ATE367447T1 true ATE367447T1 (de) 2007-08-15

Family

ID=22190591

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99923020T ATE367447T1 (de) 1998-05-13 1999-05-13 Lentivirale verpackungszellen

Country Status (11)

Country Link
US (4) US6365150B1 (de)
EP (1) EP1076715B1 (de)
AT (1) ATE367447T1 (de)
AU (1) AU3988799A (de)
CA (1) CA2328404C (de)
CY (1) CY1107760T1 (de)
DE (1) DE69936577T2 (de)
DK (1) DK1076715T3 (de)
ES (1) ES2291025T3 (de)
PT (1) PT1076715E (de)
WO (1) WO1999058701A1 (de)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020173643A1 (en) * 1995-04-14 2002-11-21 Kappes John C. Fusion protein delivery system and uses thereof
US5994136A (en) * 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
CA2328404C (en) * 1998-05-13 2007-07-24 Genetix Pharmaceuticals, Inc. Novel lentiviral packaging cells
US6555342B1 (en) 1998-06-03 2003-04-29 Uab Research Foundation Fusion protein delivery system and uses thereof
US7622300B2 (en) 1998-06-03 2009-11-24 Kappes John C Trans-lentiviral vector particles and transduction of eukaryotic cells therewith
ATE528406T1 (de) * 1999-04-29 2011-10-15 Gbp Ip Llc Verfahren und mittel zur herstellung von sicheren,rekombinanten lentivirusvektoren mit hohem titer
EP1103615A1 (de) * 1999-11-25 2001-05-30 Universite De Geneve Vektoren, die nicht-teilende Zellen immortalisieren können und Zellen, die mit diesen Vektoren immortalisiert wurden
US20040248113A1 (en) * 1999-12-28 2004-12-09 Alessandro Sette Method and system for optimizing multi-epitope nucleic acid constructs and peptides encoded thereby
AU2002311777A1 (en) * 2001-04-17 2002-10-28 Genetix Pharmaceuticals, Inc. Method of treating arthritis using lentiviral vectors in gene therapy
US20030039636A1 (en) * 2001-05-01 2003-02-27 Genetix Pharmaceuticals, Inc. Novel self-inactivating (SIN) lentiviral vectors
WO2002100317A2 (en) 2001-05-25 2002-12-19 The Trustees Of The University Of Pennsylvania Targeted particles and methods of using the same
ES2247423T5 (es) 2001-11-19 2015-12-30 Scil Technology Gmbh Método de producción de un dispositivo recubierto homogéneamente que tiene propiedades osteoinductoras y osteoconductoras
ATE322916T1 (de) * 2002-09-10 2006-04-15 Scil Technology Gmbh Unter verringerter sauerstoffkonzentration mit einem osteoinduktiven protein beschichtetes metallimplantat
KR100553154B1 (ko) * 2002-10-21 2006-02-22 유지창 Ｈｉｖ 뉴클레오캡시드 단백질의 동물세포 발현용 벡터 및 이의 제조방법
WO2004113547A2 (en) * 2003-05-19 2004-12-29 The Government Of The United States, As Represented By The Secretary Of Health And Human Services Potent combinations of mrna transport elements
FR2872170B1 (fr) 2004-06-25 2006-11-10 Centre Nat Rech Scient Cnrse Lentivirus non interactif et non replicatif, preparation et utilisations
WO2010127166A2 (en) 2009-04-30 2010-11-04 The Regents Of The University Of California Combination anti-hiv vectors, targeting vectors, and methods of use
AU2011230619C1 (en) 2010-03-25 2016-06-23 Oregon Health & Science University CMV glycoproteins and recombinant vectors
HUE037408T2 (hu) 2011-06-10 2018-08-28 Univ Oregon Health & Science CMV glikoproteinek és rekombináns vektorok
WO2014026053A1 (en) 2012-08-09 2014-02-13 The Regents Of The University Of California Cd25 pre-selective combination anti-hiv vectors, targeting vectors, and methods of use
US20140065110A1 (en) 2012-08-31 2014-03-06 The Regents Of The University Of California Genetically modified msc and therapeutic methods
US20160251680A1 (en) * 2013-11-05 2016-09-01 Clontech Laboratories, Inc. Dry transfection compositions and methods for making and using the same
MX375592B (es) 2014-04-25 2025-03-06 The Children´S Medical Center Corp Composiciones y su uso para tratar hemoglobinopatias.
JP2019509275A (ja) 2016-02-23 2019-04-04 イミューン デザイン コーポレイション マルチゲノムレトロウイルスベクター調製物ならびにそれらを産生および使用するための方法およびシステム
EP3276006A1 (de) * 2016-07-27 2018-01-31 Theravectys Lentivirale vektoren zur expression von hepatitis-b-virus (hbv)-antigenen
WO2019213013A1 (en) 2018-05-02 2019-11-07 The Children's Medical Center Corporation Improved bcl11a micrornas for treating hemoglobinopathies
JP7470119B2 (ja) 2018-08-24 2024-04-17 シーエスエル ベーリング ジーン セラピー インコーポレイテッド 無血清培地におけるベクター産生
WO2020102709A1 (en) * 2018-11-16 2020-05-22 The Regents Of The University Of California Compositions and methods for delivering crispr/cas effector polypeptides
US11912986B2 (en) 2019-09-23 2024-02-27 The Regents Of The University Of California Methods for screening genetic perturbations
EP4277933A4 (de) 2021-01-14 2024-12-11 Senti Biosciences, Inc. Sekretierbare nutzlastregulierung
CN113186353A (zh) * 2021-05-28 2021-07-30 杭州晶佰生物技术有限公司 针对c型逆转录病毒的数字pcr检测用引物及检测方法
US12565647B2 (en) 2021-05-28 2026-03-03 The Regents Of The University Of California Compositions and methods for targeted delivery of CRISPR-Cas effector polypeptides and transgenes
WO2022261149A2 (en) 2021-06-09 2022-12-15 Scribe Therapeutics Inc. Particle delivery systems

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5861161A (en) 1994-09-07 1999-01-19 Universite De Montreal Chimeric proteins comprising a Vpr/Vpx virion incorporation domain for targeting into HIV-1 or HIV-2 virions
ATE408015T1 (de) 1995-04-14 2008-09-15 Univ Alabama Res Found Transfer system basierend auf einem fusionsprotein und seine anwendungen.
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
CA2237000A1 (en) 1995-11-08 1997-05-15 The Whitehead Institute For Biomedical Research Stable packaging cell line producing pseudotyped retroviruses
EP0970201A1 (de) * 1996-09-17 2000-01-12 The Salk Institute For Biological Studies Retrovirale vektoren, die sich nicht teilende zellen transduzieren können
US5908773A (en) 1996-09-20 1999-06-01 Cornell Research Foundation, Inc. KSHV positive cell lines
GB9621679D0 (en) 1996-10-17 1996-12-11 Oxford Biomedica Ltd Improved retroviral vectors
PT904392E (pt) * 1996-10-17 2001-06-29 Oxford Biomedica Ltd Vectores retrovirais
CA2328404C (en) 1998-05-13 2007-07-24 Genetix Pharmaceuticals, Inc. Novel lentiviral packaging cells

Also Published As

Publication number Publication date
EP1076715B1 (de) 2007-07-18
CY1107760T1 (el) 2013-06-19
CA2328404C (en) 2007-07-24
DE69936577D1 (de) 2007-08-30
CA2328404A1 (en) 1999-11-18
US8034620B2 (en) 2011-10-11
US6955919B2 (en) 2005-10-18
US6365150B1 (en) 2002-04-02
US7311907B2 (en) 2007-12-25
EP1076715A1 (de) 2001-02-21
ES2291025T3 (es) 2008-02-16
US20050170507A1 (en) 2005-08-04
AU3988799A (en) 1999-11-29
WO1999058701A1 (en) 1999-11-18
DK1076715T3 (da) 2007-11-19
US20020168346A1 (en) 2002-11-14
US20080187997A1 (en) 2008-08-07
DE69936577T2 (de) 2008-04-30
PT1076715E (pt) 2007-10-26

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