ATE368049T1 - Nukleotidderivate mit einer reportergruppe und einer polymerase-enzym-blockierende gruppe - Google Patents
Nukleotidderivate mit einer reportergruppe und einer polymerase-enzym-blockierende gruppeInfo
- Publication number
- ATE368049T1 ATE368049T1 AT01936624T AT01936624T ATE368049T1 AT E368049 T1 ATE368049 T1 AT E368049T1 AT 01936624 T AT01936624 T AT 01936624T AT 01936624 T AT01936624 T AT 01936624T AT E368049 T1 ATE368049 T1 AT E368049T1
- Authority
- AT
- Austria
- Prior art keywords
- group
- sup
- polymerase enzyme
- nucleotide derivatives
- enzyme blocking
- Prior art date
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 4
- 108090000790 Enzymes Proteins 0.000 title abstract 4
- 230000000903 blocking effect Effects 0.000 title abstract 4
- 239000002773 nucleotide Substances 0.000 title abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0013276.1A GB0013276D0 (en) | 2000-06-01 | 2000-06-01 | Nucleotide analogues |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE368049T1 true ATE368049T1 (de) | 2007-08-15 |
Family
ID=9892749
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01936624T ATE368049T1 (de) | 2000-06-01 | 2001-05-31 | Nukleotidderivate mit einer reportergruppe und einer polymerase-enzym-blockierende gruppe |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7078499B2 (de) |
| EP (1) | EP1287012B1 (de) |
| JP (1) | JP2003535102A (de) |
| AT (1) | ATE368049T1 (de) |
| AU (2) | AU6249501A (de) |
| CA (1) | CA2408143A1 (de) |
| DE (1) | DE60129553D1 (de) |
| GB (1) | GB0013276D0 (de) |
| IL (1) | IL152673A0 (de) |
| WO (1) | WO2001092284A1 (de) |
Families Citing this family (63)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| US7501245B2 (en) | 1999-06-28 | 2009-03-10 | Helicos Biosciences Corp. | Methods and apparatuses for analyzing polynucleotide sequences |
| AU2001296645A1 (en) | 2000-10-06 | 2002-04-15 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding dna and rna |
| US9708358B2 (en) | 2000-10-06 | 2017-07-18 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
| JP2004523243A (ja) | 2001-03-12 | 2004-08-05 | カリフォルニア インスティチュート オブ テクノロジー | 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置 |
| DE10120797B4 (de) * | 2001-04-27 | 2005-12-22 | Genovoxx Gmbh | Verfahren zur Analyse von Nukleinsäureketten |
| WO2003006625A2 (en) * | 2001-07-13 | 2003-01-23 | Ambergen, Inc. | Nucleotide compositions comprising photocleavable markers and methods of preparation thereof |
| GB0128526D0 (en) * | 2001-11-29 | 2002-01-23 | Amersham Pharm Biotech Uk Ltd | Nucleotide analogues |
| GB0129012D0 (en) | 2001-12-04 | 2002-01-23 | Solexa Ltd | Labelled nucleotides |
| US7057026B2 (en) * | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| US7074597B2 (en) | 2002-07-12 | 2006-07-11 | The Trustees Of Columbia University In The City Of New York | Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry |
| US11008359B2 (en) | 2002-08-23 | 2021-05-18 | Illumina Cambridge Limited | Labelled nucleotides |
| WO2004018497A2 (en) | 2002-08-23 | 2004-03-04 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
| US7414116B2 (en) | 2002-08-23 | 2008-08-19 | Illumina Cambridge Limited | Labelled nucleotides |
| PT3438116T (pt) | 2002-08-23 | 2021-03-23 | Illumina Cambridge Ltd | Nucleótidos identificados |
| GB0324456D0 (en) | 2003-10-20 | 2003-11-19 | Isis Innovation | Parallel DNA sequencing methods |
| EP1725572B1 (de) | 2003-11-05 | 2017-05-31 | AGCT GmbH | Makromolekulare nukleotidverbindungen und methoden zu deren anwendung |
| GB0326073D0 (en) * | 2003-11-07 | 2003-12-10 | Solexa Ltd | Improvements in or relating to polynucleotide arrays |
| US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
| WO2005080605A2 (en) | 2004-02-19 | 2005-09-01 | Helicos Biosciences Corporation | Methods and kits for analyzing polynucleotide sequences |
| EP2436778A3 (de) | 2004-03-03 | 2012-07-11 | The Trustees of Columbia University in the City of New York | Photospaltbare fluoreszierende Nukleotide zur DNA-Sequenzierung auf einem durch ortsspezifische Kopplungschemie konstruierten Chip |
| US7476734B2 (en) | 2005-12-06 | 2009-01-13 | Helicos Biosciences Corporation | Nucleotide analogs |
| US7635562B2 (en) | 2004-05-25 | 2009-12-22 | Helicos Biosciences Corporation | Methods and devices for nucleic acid sequence determination |
| US7220549B2 (en) | 2004-12-30 | 2007-05-22 | Helicos Biosciences Corporation | Stabilizing a nucleic acid for nucleic acid sequencing |
| US7482120B2 (en) | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
| US9169510B2 (en) | 2005-06-21 | 2015-10-27 | The Trustees Of Columbia University In The City Of New York | Pyrosequencing methods and related compositions |
| GB0517097D0 (en) | 2005-08-19 | 2005-09-28 | Solexa Ltd | Modified nucleosides and nucleotides and uses thereof |
| US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
| US7982029B2 (en) | 2005-10-31 | 2011-07-19 | The Trustees Of Columbia University In The City Of New York | Synthesis of four color 3′O-allyl, modified photocleavable fluorescent nucleotides and related methods |
| GB2446083B (en) | 2005-10-31 | 2011-03-02 | Univ Columbia | Chemically cleavable 3'-0-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods |
| US7397546B2 (en) | 2006-03-08 | 2008-07-08 | Helicos Biosciences Corporation | Systems and methods for reducing detected intensity non-uniformity in a laser beam |
| US7772390B1 (en) | 2006-07-18 | 2010-08-10 | The Regents Of The University Of California | Lipid mediated nucleic acid synthesis |
| WO2008042067A2 (en) | 2006-09-28 | 2008-04-10 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
| GB2457402B (en) | 2006-12-01 | 2011-10-19 | Univ Columbia | Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators |
| US11940413B2 (en) | 2007-02-05 | 2024-03-26 | IsoPlexis Corporation | Methods and devices for sequencing nucleic acids in smaller batches |
| US8481259B2 (en) | 2007-02-05 | 2013-07-09 | Intelligent Bio-Systems, Inc. | Methods and devices for sequencing nucleic acids in smaller batches |
| US11035823B2 (en) | 2009-03-17 | 2021-06-15 | Qiagen Sciences, Llc | Methods and devices for sequencing nucleic acids in smaller batches |
| DK2725107T3 (da) | 2007-10-19 | 2019-01-02 | Univ Columbia | DNA-sekventering med ikke-fluorescerende nukleotidreversible terminatorer og ddNTP'er modificeret med spaltbart mærke og nukleinsyre omfattende inosin med reversible terminatorer |
| WO2009051807A1 (en) | 2007-10-19 | 2009-04-23 | The Trustees Of Columbia University In The City Of New York | Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis |
| WO2010111674A2 (en) | 2009-03-27 | 2010-09-30 | Life Technologies Corporation | Methods and apparatus for single molecule sequencing using energy transfer detection |
| US20100330569A1 (en) * | 2009-04-23 | 2010-12-30 | Intelligent Bio-Systems, Inc. | Hydroxymethyl Linkers For Labeling Nucleotides |
| EP2494073B1 (de) | 2009-10-26 | 2017-11-29 | AGCT GmbH | Konjugate von nukleotiden und methoden zu deren anwendung |
| US8603741B2 (en) | 2010-02-18 | 2013-12-10 | Pacific Biosciences Of California, Inc. | Single molecule sequencing with two distinct chemistry steps |
| DE102012008375A1 (de) | 2011-04-27 | 2012-10-31 | Genovoxx Gmbh | Methoden und Komponenten zur Detektion von Nukleinsäureketten |
| WO2012150035A1 (de) | 2011-05-04 | 2012-11-08 | Genovoxx Gmbh | Nukleosid-triphosphat-konjugate und methoden zu deren anwendung |
| US9624539B2 (en) | 2011-05-23 | 2017-04-18 | The Trustees Of Columbia University In The City Of New York | DNA sequencing by synthesis using Raman and infrared spectroscopy detection |
| WO2014139596A1 (en) | 2013-03-15 | 2014-09-18 | Illumina Cambridge Limited | Modified nucleosides or nucleotides |
| WO2014144883A1 (en) | 2013-03-15 | 2014-09-18 | The Trustees Of Columbia University In The City Of New York | Raman cluster tagged molecules for biological imaging |
| FR3020071B1 (fr) | 2014-04-17 | 2017-12-22 | Dna Script | Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede |
| FR3025201B1 (fr) | 2014-09-02 | 2018-10-12 | Dna Script | Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques |
| US10190162B2 (en) | 2014-10-23 | 2019-01-29 | Complete Genomics, Inc. | Signal confinement sequencing (SCS) and nucleotide analogues for signal confinement sequencing |
| US11390856B2 (en) | 2017-08-07 | 2022-07-19 | Dna Script | Variants of family a DNA polymerase and uses thereof |
| US10752887B2 (en) | 2018-01-08 | 2020-08-25 | Dna Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| US10435676B2 (en) | 2018-01-08 | 2019-10-08 | Dna Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| EP3826762A1 (de) | 2018-07-23 | 2021-06-02 | DNA Script | Massiv parallele enzymatische synthese von nukleinsäuresträngen |
| WO2020036926A1 (en) | 2018-08-17 | 2020-02-20 | Cellecta, Inc. | Multiplex preparation of barcoded gene specific dna fragments |
| SG11202105441WA (en) | 2018-12-13 | 2021-06-29 | Dna Script | Direct oligonucleotide synthesis on cells and biomolecules |
| US11293061B2 (en) | 2018-12-26 | 2022-04-05 | Illumina Cambridge Limited | Sequencing methods using nucleotides with 3′ AOM blocking group |
| WO2020165137A1 (en) | 2019-02-12 | 2020-08-20 | Dna Script | Efficient product cleavage in template-free enzymatic synthesis of polynucleotides. |
| US11787831B2 (en) | 2020-06-22 | 2023-10-17 | Illumina Cambridge Limited | Nucleosides and nucleotides with 3′ acetal blocking group |
| US12435369B2 (en) * | 2021-08-13 | 2025-10-07 | Singular Genomcis Systems, Inc. | Nucleotide analogs and uses thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5268486A (en) | 1986-04-18 | 1993-12-07 | Carnegie-Mellon Unversity | Method for labeling and detecting materials employing arylsulfonate cyanine dyes |
| WO1993005183A1 (en) * | 1991-09-09 | 1993-03-18 | Baylor College Of Medicine | Method and device for rapid dna or rna sequencing determination by a base addition sequencing scheme |
| US5681940A (en) * | 1994-11-02 | 1997-10-28 | Icn Pharmaceuticals | Sugar modified nucleosides and oligonucleotides |
| US6147205A (en) * | 1995-12-15 | 2000-11-14 | Affymetrix, Inc. | Photocleavable protecting groups and methods for their use |
-
2000
- 2000-06-01 GB GBGB0013276.1A patent/GB0013276D0/en not_active Ceased
-
2001
- 2001-05-31 CA CA002408143A patent/CA2408143A1/en not_active Abandoned
- 2001-05-31 AU AU6249501A patent/AU6249501A/xx active Pending
- 2001-05-31 AU AU2001262495A patent/AU2001262495B2/en not_active Ceased
- 2001-05-31 JP JP2002500897A patent/JP2003535102A/ja active Pending
- 2001-05-31 AT AT01936624T patent/ATE368049T1/de not_active IP Right Cessation
- 2001-05-31 WO PCT/GB2001/002402 patent/WO2001092284A1/en not_active Ceased
- 2001-05-31 IL IL15267301A patent/IL152673A0/xx unknown
- 2001-05-31 DE DE60129553T patent/DE60129553D1/de not_active Expired - Lifetime
- 2001-05-31 EP EP01936624A patent/EP1287012B1/de not_active Expired - Lifetime
- 2001-05-31 US US10/276,759 patent/US7078499B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| AU6249501A (en) | 2001-12-11 |
| CA2408143A1 (en) | 2001-12-06 |
| US20030194722A1 (en) | 2003-10-16 |
| EP1287012A1 (de) | 2003-03-05 |
| IL152673A0 (en) | 2003-06-24 |
| DE60129553D1 (de) | 2007-09-06 |
| AU2001262495B2 (en) | 2006-02-02 |
| WO2001092284A1 (en) | 2001-12-06 |
| GB0013276D0 (en) | 2000-07-26 |
| JP2003535102A (ja) | 2003-11-25 |
| US7078499B2 (en) | 2006-07-18 |
| EP1287012B1 (de) | 2007-07-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |