ATE380878T1 - Verfahren und zusammensetzungen für die proteinexpression und reinigung - Google Patents

Verfahren und zusammensetzungen für die proteinexpression und reinigung

Info

Publication number
ATE380878T1
ATE380878T1 AT03703721T AT03703721T ATE380878T1 AT E380878 T1 ATE380878 T1 AT E380878T1 AT 03703721 T AT03703721 T AT 03703721T AT 03703721 T AT03703721 T AT 03703721T AT E380878 T1 ATE380878 T1 AT E380878T1
Authority
AT
Austria
Prior art keywords
methods
purification
compositions
protein expression
secretion
Prior art date
Application number
AT03703721T
Other languages
English (en)
Inventor
Tauseef Butt
Steven Weeks
Hiep Tran
Michael Malakhov
Oxana Malakhova
Original Assignee
Lifesensors Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lifesensors Inc filed Critical Lifesensors Inc
Application granted granted Critical
Publication of ATE380878T1 publication Critical patent/ATE380878T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
AT03703721T 2002-01-07 2003-01-07 Verfahren und zusammensetzungen für die proteinexpression und reinigung ATE380878T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US34644902P 2002-01-07 2002-01-07

Publications (1)

Publication Number Publication Date
ATE380878T1 true ATE380878T1 (de) 2007-12-15

Family

ID=23359446

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03703721T ATE380878T1 (de) 2002-01-07 2003-01-07 Verfahren und zusammensetzungen für die proteinexpression und reinigung

Country Status (8)

Country Link
US (3) US7060461B2 (de)
EP (1) EP1470236B1 (de)
JP (1) JP2005514025A (de)
AT (1) ATE380878T1 (de)
AU (1) AU2003205053B2 (de)
CA (1) CA2472477C (de)
DE (1) DE60318014T2 (de)
WO (1) WO2003057174A2 (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60238532D1 (de) * 2001-05-04 2011-01-20 Cornell Res Foundation Inc Schnell spaltbares sumofusionsprotein-expressionssystem für schwer zu exprimierende proteine
CA2472477C (en) * 2002-01-07 2012-03-20 Lifesensors, Inc. Methods and compositions for protein expression and purification
AU2004254600A1 (en) * 2003-06-26 2005-01-13 Lifesensors, Inc. Methods and compositions for enhanced protein expression and purification
EP2426140A1 (de) 2003-08-15 2012-03-07 University of Florida Research Foundation, Inc. Identifizierung von Porphyromonas Gingivalis Virulenz-Polynukleotiden bei der Diagnose, Behandlung und Überwachung von Periodontalerkrankungen
CA2543257C (en) 2003-10-24 2013-12-31 Gencia Corporation Methods and compositions for delivering polynucleotides
US20090123468A1 (en) 2003-10-24 2009-05-14 Gencia Corporation Transducible polypeptides for modifying metabolism
US8062891B2 (en) 2003-10-24 2011-11-22 Gencia Corporation Nonviral vectors for delivering polynucleotides to plants
US8507277B2 (en) 2003-10-24 2013-08-13 Gencia Corporation Nonviral vectors for delivering polynucleotides
US8133733B2 (en) 2003-10-24 2012-03-13 Gencia Corporation Nonviral vectors for delivering polynucleotides to target tissues
ES2306541B1 (es) * 2004-04-16 2009-09-28 Consejo Superior Investig. Cientificas Trasplante funcional de la maquinaria de sumoilacion a escherichia coli.
EP1836214A4 (de) * 2004-12-30 2009-01-21 Lifesensors Inc Zusammensetzungen, verfahren und kits zur verbesserung von proteinexpression, -löslichkeit und -isolierung
US20100048480A1 (en) * 2006-11-22 2010-02-25 Bettina Bommarius Production of anti-microbial peptides
WO2008065673A1 (en) * 2006-11-30 2008-06-05 Biocon Limited A method for producing n-terminal methioine free polypeptides in microbial host cells
GB0624071D0 (en) * 2006-12-01 2007-01-10 Bioline Res Ltd Purification methods and materials
US20120171707A1 (en) * 2009-09-11 2012-07-05 Albert Einstein College Of Medicine Of Yeshiva University Assay for inhibitors of sumo and sumo-targeted ubiquitin ligase pathway
WO2013103964A1 (en) 2012-01-06 2013-07-11 Gencia Corporation Methods and compositions for treating proteinopathies
ES2582802T3 (es) 2012-01-23 2016-09-15 Morphosys Ag Uso de lisozima como marcador
WO2013188873A1 (en) 2012-06-15 2013-12-19 Gencia Corporation Compositions and methods for enhancing immune responses
WO2013188874A1 (en) 2012-06-15 2013-12-19 Gencia Corporation Methods of mitigating side effects of radiation exposure and chemotherapy
AR094276A1 (es) * 2012-12-21 2015-07-22 Alkem Laboratories Ltd Marcadores de fusion y sistema de vector de expresion para la expresion de hormona paratiroides humana (rhpth)
US9707244B2 (en) 2013-03-15 2017-07-18 Gencia Corporation Compositions and methods for treating conditions that affect epidermis
US9970945B2 (en) 2013-06-07 2018-05-15 Georgia State University Research Foundtion, Inc. Compositions, methods, and assays using proteo-liposome-transfected oocytes for analyzing the structure, function, and activity of membrane proteins
US9447157B2 (en) 2013-09-13 2016-09-20 Augusta University Research Institute, Inc. Nitration shielding peptides and methods of use thereof
WO2015049230A1 (en) 2013-10-01 2015-04-09 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. An orthogonal set of tag-cleaving proteases for purification of proteins and stoichiometric protein complexes
JPWO2015079868A1 (ja) * 2013-11-28 2017-03-16 国立大学法人佐賀大学 リグノセルロース分解物耐性微生物およびその製造方法、ならびにその微生物を用いてバイオエタノールを製造する方法
US10378003B2 (en) 2014-11-10 2019-08-13 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Tag removal from proteins expressed in pro- and eukaryotic hosts
BR112017021016A2 (pt) * 2015-03-31 2018-07-03 Yeda Research And Development Co Ltd preparação de proteína, composição farmacêutica, proteína de fusão, polinucleotídeo isolado, estrutura de ácido nucleico, método para tratamento de doença ou condição associada ao excesso de glutamato, e método de purificação de um polipeptídeo
IL272635B2 (en) 2017-09-06 2024-02-01 Univ Yale Variants of interleukin-18 and methods of use
CN107988259B (zh) * 2018-01-12 2020-01-21 中国科学院生物物理研究所 SmartBac杆状病毒表达系统及其应用
JP2020143000A (ja) * 2019-03-04 2020-09-10 大陽日酸株式会社 融合タンパク質及びその製造方法、目的タンパク質の製造方法
CN112898407B (zh) * 2021-01-25 2022-07-12 南京医科大学 重组驼源血清白蛋白的制备方法
CN114262697B (zh) * 2021-12-30 2022-12-13 南京巨匠生物科技有限公司 融合Bsu DNA聚合酶和Bsu DNA聚合酶突变体及其基因、质粒、基因工程菌
US20250161429A1 (en) 2022-02-14 2025-05-22 University Of Georgia Research Foundation, Inc. Pan-pneumovirus vaccine compositions and methods of use thereof
CN120137057A (zh) * 2025-03-14 2025-06-13 中国科学院深圳先进技术研究院 蛋白质相分离调节系统及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2295322C (en) * 1997-06-19 2009-05-26 University Of Medicine And Dentistry Of New Jersey Methods and compositions for rapid purification of proteasomes and methods of use of components thereof
WO1999013077A2 (en) * 1997-09-10 1999-03-18 President And Fellows Of Harvard College Inducible methods for repressing gene function
DE60238532D1 (de) * 2001-05-04 2011-01-20 Cornell Res Foundation Inc Schnell spaltbares sumofusionsprotein-expressionssystem für schwer zu exprimierende proteine
US7220576B2 (en) 2002-01-07 2007-05-22 Lifesensors, Inc. Methods and compositions for protein expression and purification
CA2472477C (en) 2002-01-07 2012-03-20 Lifesensors, Inc. Methods and compositions for protein expression and purification

Also Published As

Publication number Publication date
DE60318014D1 (de) 2008-01-24
DE60318014T2 (de) 2008-11-27
CA2472477C (en) 2012-03-20
WO2003057174A2 (en) 2003-07-17
AU2003205053B2 (en) 2008-10-16
US20070259414A1 (en) 2007-11-08
US20060216785A1 (en) 2006-09-28
CA2472477A1 (en) 2003-07-17
JP2005514025A (ja) 2005-05-19
WO2003057174A3 (en) 2004-03-04
US7060461B2 (en) 2006-06-13
EP1470236A4 (de) 2005-10-19
US20030153045A1 (en) 2003-08-14
EP1470236B1 (de) 2007-12-12
EP1470236A2 (de) 2004-10-27
AU2003205053A1 (en) 2003-07-24
US7820384B2 (en) 2010-10-26

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