ATE380878T1 - Verfahren und zusammensetzungen für die proteinexpression und reinigung - Google Patents
Verfahren und zusammensetzungen für die proteinexpression und reinigungInfo
- Publication number
- ATE380878T1 ATE380878T1 AT03703721T AT03703721T ATE380878T1 AT E380878 T1 ATE380878 T1 AT E380878T1 AT 03703721 T AT03703721 T AT 03703721T AT 03703721 T AT03703721 T AT 03703721T AT E380878 T1 ATE380878 T1 AT E380878T1
- Authority
- AT
- Austria
- Prior art keywords
- methods
- purification
- compositions
- protein expression
- secretion
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 102000037865 fusion proteins Human genes 0.000 abstract 1
- 108020001507 fusion proteins Proteins 0.000 abstract 1
- 230000028327 secretion Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Detergent Compositions (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US34644902P | 2002-01-07 | 2002-01-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE380878T1 true ATE380878T1 (de) | 2007-12-15 |
Family
ID=23359446
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03703721T ATE380878T1 (de) | 2002-01-07 | 2003-01-07 | Verfahren und zusammensetzungen für die proteinexpression und reinigung |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US7060461B2 (de) |
| EP (1) | EP1470236B1 (de) |
| JP (1) | JP2005514025A (de) |
| AT (1) | ATE380878T1 (de) |
| AU (1) | AU2003205053B2 (de) |
| CA (1) | CA2472477C (de) |
| DE (1) | DE60318014T2 (de) |
| WO (1) | WO2003057174A2 (de) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2002257243A1 (en) * | 2001-05-04 | 2002-11-18 | Cornell Research Foundation, Inc. | Rapidly cleavable sumo fusion protein expression system for difficult to express proteins |
| DE60318014T2 (de) * | 2002-01-07 | 2008-11-27 | Lifesensors, Inc. | Verfahren und zusammensetzungen für die proteinexpression und reinigung |
| AU2004254600A1 (en) * | 2003-06-26 | 2005-01-13 | Lifesensors, Inc. | Methods and compositions for enhanced protein expression and purification |
| ATE495189T1 (de) | 2003-08-15 | 2011-01-15 | Univ Florida | Die identifikation von polynukleotiden von porphyromonas gingivalis-virulenz zur diagnose, behandlung und beobachtung von periodontalerkrankungen |
| AU2004297533B2 (en) | 2003-10-24 | 2010-04-29 | Gencia Corporation | Methods and compositions for delivering polynucleotides |
| US8507277B2 (en) | 2003-10-24 | 2013-08-13 | Gencia Corporation | Nonviral vectors for delivering polynucleotides |
| US8062891B2 (en) | 2003-10-24 | 2011-11-22 | Gencia Corporation | Nonviral vectors for delivering polynucleotides to plants |
| US20090123468A1 (en) | 2003-10-24 | 2009-05-14 | Gencia Corporation | Transducible polypeptides for modifying metabolism |
| US8133733B2 (en) | 2003-10-24 | 2012-03-13 | Gencia Corporation | Nonviral vectors for delivering polynucleotides to target tissues |
| ES2306541B1 (es) * | 2004-04-16 | 2009-09-28 | Consejo Superior Investig. Cientificas | Trasplante funcional de la maquinaria de sumoilacion a escherichia coli. |
| EP1836214A4 (de) * | 2004-12-30 | 2009-01-21 | Lifesensors Inc | Zusammensetzungen, verfahren und kits zur verbesserung von proteinexpression, -löslichkeit und -isolierung |
| CA2670014A1 (en) * | 2006-11-22 | 2008-11-20 | Emory University | Production of anti-microbial peptides |
| EP2097520A4 (de) * | 2006-11-30 | 2012-07-04 | Biocon Ltd | Verfahren zur herstellung von polypeptiden ohne n-terminales methionin in mikrobiellen wirtszellen |
| GB0624071D0 (en) * | 2006-12-01 | 2007-01-10 | Bioline Res Ltd | Purification methods and materials |
| US20120171707A1 (en) * | 2009-09-11 | 2012-07-05 | Albert Einstein College Of Medicine Of Yeshiva University | Assay for inhibitors of sumo and sumo-targeted ubiquitin ligase pathway |
| WO2013103964A1 (en) | 2012-01-06 | 2013-07-11 | Gencia Corporation | Methods and compositions for treating proteinopathies |
| EP2807179B1 (de) | 2012-01-23 | 2016-04-20 | MorphoSys AG | Verwendung von lysozym als tag |
| WO2013188874A1 (en) | 2012-06-15 | 2013-12-19 | Gencia Corporation | Methods of mitigating side effects of radiation exposure and chemotherapy |
| WO2013188873A1 (en) | 2012-06-15 | 2013-12-19 | Gencia Corporation | Compositions and methods for enhancing immune responses |
| AR094276A1 (es) * | 2012-12-21 | 2015-07-22 | Alkem Laboratories Ltd | Marcadores de fusion y sistema de vector de expresion para la expresion de hormona paratiroides humana (rhpth) |
| CN105377265A (zh) | 2013-03-15 | 2016-03-02 | 吉奇亚公司 | 用于治疗影响表皮的病状的组合物和方法 |
| WO2014197905A1 (en) | 2013-06-07 | 2014-12-11 | Georgia State University Research Foundation, Inc. | Compositions, methods, and assays for analyzing the structure, function, and activity of membrane proteins |
| US9447157B2 (en) | 2013-09-13 | 2016-09-20 | Augusta University Research Institute, Inc. | Nitration shielding peptides and methods of use thereof |
| WO2015049230A1 (en) | 2013-10-01 | 2015-04-09 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | An orthogonal set of tag-cleaving proteases for purification of proteins and stoichiometric protein complexes |
| WO2015079868A1 (ja) * | 2013-11-28 | 2015-06-04 | 国立大学法人佐賀大学 | リグノセルロース分解物耐性微生物およびその製造方法、ならびにその微生物を用いてバイオエタノールを製造する方法 |
| EP3218472B1 (de) | 2014-11-10 | 2020-07-08 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Markierungsentfernung von in pro- und eukaryotischen wirtszellen exprimierten proteinen |
| KR20170132293A (ko) * | 2015-03-31 | 2017-12-01 | 예다 리서치 앤드 디벨럽먼트 캄파니 리미티드 | 글루타메이트 옥살로아세테이트 트랜스아미나제 1（ｇｏｔ１） 제제와 이의 제조방법 및 용도 |
| JP7795181B2 (ja) | 2017-09-06 | 2026-01-07 | イェール ユニバーシティー | インターロイキン－１８バリアントとその利用法 |
| CN107988259B (zh) * | 2018-01-12 | 2020-01-21 | 中国科学院生物物理研究所 | SmartBac杆状病毒表达系统及其应用 |
| JP2020143000A (ja) * | 2019-03-04 | 2020-09-10 | 大陽日酸株式会社 | 融合タンパク質及びその製造方法、目的タンパク質の製造方法 |
| CN112898407B (zh) * | 2021-01-25 | 2022-07-12 | 南京医科大学 | 重组驼源血清白蛋白的制备方法 |
| CN114262697B (zh) * | 2021-12-30 | 2022-12-13 | 南京巨匠生物科技有限公司 | 融合Bsu DNA聚合酶和Bsu DNA聚合酶突变体及其基因、质粒、基因工程菌 |
| AU2023219791A1 (en) | 2022-02-14 | 2024-08-22 | University Of Georgia Research Foundation, Inc. | Pan-pneumovirus vaccine compositions and methods of use thereof. |
| CN118206630B (zh) * | 2024-02-06 | 2026-06-02 | 四川大学 | 一种重组人白介素-2或/和其突变体在原核体系中可溶性表达及纯化的方法 |
| WO2026107236A1 (en) | 2024-11-13 | 2026-05-21 | University Of Georgia Research Foundation, Inc. | Compositions and methods for vaccination against mycobacterium tuberculosis |
| CN120137057B (zh) * | 2025-03-14 | 2026-06-16 | 中国科学院深圳先进技术研究院 | 蛋白质相分离调节系统及其应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69827268T2 (de) * | 1997-06-19 | 2005-12-01 | University Of Medicine And Dentistry Of New Jersey | Verfahren und zusammensetzungen zur raschen aufreinigung von proteasomen und verfahren zur verwendung entsprechender komponenten |
| WO1999013077A2 (en) | 1997-09-10 | 1999-03-18 | President And Fellows Of Harvard College | Inducible methods for repressing gene function |
| AU2002257243A1 (en) * | 2001-05-04 | 2002-11-18 | Cornell Research Foundation, Inc. | Rapidly cleavable sumo fusion protein expression system for difficult to express proteins |
| US7220576B2 (en) | 2002-01-07 | 2007-05-22 | Lifesensors, Inc. | Methods and compositions for protein expression and purification |
| DE60318014T2 (de) | 2002-01-07 | 2008-11-27 | Lifesensors, Inc. | Verfahren und zusammensetzungen für die proteinexpression und reinigung |
-
2003
- 2003-01-07 DE DE60318014T patent/DE60318014T2/de not_active Expired - Lifetime
- 2003-01-07 EP EP03703721A patent/EP1470236B1/de not_active Expired - Lifetime
- 2003-01-07 AU AU2003205053A patent/AU2003205053B2/en not_active Ceased
- 2003-01-07 JP JP2003557532A patent/JP2005514025A/ja active Pending
- 2003-01-07 AT AT03703721T patent/ATE380878T1/de not_active IP Right Cessation
- 2003-01-07 CA CA2472477A patent/CA2472477C/en not_active Expired - Lifetime
- 2003-01-07 WO PCT/US2003/000436 patent/WO2003057174A2/en not_active Ceased
- 2003-01-07 US US10/338,411 patent/US7060461B2/en not_active Expired - Lifetime
-
2006
- 2006-02-28 US US11/363,660 patent/US7820384B2/en not_active Expired - Fee Related
-
2007
- 2007-05-21 US US11/751,441 patent/US20070259414A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CA2472477A1 (en) | 2003-07-17 |
| US7060461B2 (en) | 2006-06-13 |
| EP1470236A4 (de) | 2005-10-19 |
| AU2003205053B2 (en) | 2008-10-16 |
| DE60318014T2 (de) | 2008-11-27 |
| EP1470236B1 (de) | 2007-12-12 |
| US20070259414A1 (en) | 2007-11-08 |
| JP2005514025A (ja) | 2005-05-19 |
| US7820384B2 (en) | 2010-10-26 |
| US20060216785A1 (en) | 2006-09-28 |
| WO2003057174A3 (en) | 2004-03-04 |
| US20030153045A1 (en) | 2003-08-14 |
| WO2003057174A2 (en) | 2003-07-17 |
| EP1470236A2 (de) | 2004-10-27 |
| DE60318014D1 (de) | 2008-01-24 |
| CA2472477C (en) | 2012-03-20 |
| AU2003205053A1 (en) | 2003-07-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |