ATE386810T1 - Rekombination von nukleinsäurebibliotheksmitgliedern - Google Patents
Rekombination von nukleinsäurebibliotheksmitgliedernInfo
- Publication number
- ATE386810T1 ATE386810T1 AT03734584T AT03734584T ATE386810T1 AT E386810 T1 ATE386810 T1 AT E386810T1 AT 03734584 T AT03734584 T AT 03734584T AT 03734584 T AT03734584 T AT 03734584T AT E386810 T1 ATE386810 T1 AT E386810T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- recombination
- library members
- acid library
- encodes
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/565—Complementarity determining region [CDR]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US38903202P | 2002-06-14 | 2002-06-14 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE386810T1 true ATE386810T1 (de) | 2008-03-15 |
Family
ID=29736578
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03734584T ATE386810T1 (de) | 2002-06-14 | 2003-06-16 | Rekombination von nukleinsäurebibliotheksmitgliedern |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20030232395A1 (de) |
| EP (1) | EP1558741B1 (de) |
| AT (1) | ATE386810T1 (de) |
| AU (2) | AU2003240005B2 (de) |
| CA (1) | CA2489595C (de) |
| DE (1) | DE60319259T2 (de) |
| WO (1) | WO2003106639A2 (de) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6696251B1 (en) | 1996-05-31 | 2004-02-24 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
| US6759243B2 (en) * | 1998-01-20 | 2004-07-06 | Board Of Trustees Of The University Of Illinois | High affinity TCR proteins and methods |
| WO2002061071A2 (en) * | 2000-12-18 | 2002-08-08 | Dyax Corp. | Focused libraries of genetic packages |
| WO2002059297A2 (en) | 2001-01-25 | 2002-08-01 | Evolva Biotech A/S | A library of a collection of cells |
| US8008459B2 (en) | 2001-01-25 | 2011-08-30 | Evolva Sa | Concatemers of differentially expressed multiple genes |
| DK2093286T3 (da) | 2001-10-01 | 2013-05-13 | Dyax Corp | Flerkædede, eukaryote displayvektorer og anvendelser deraf |
| WO2004016791A1 (en) * | 2002-08-01 | 2004-02-26 | Evolva Ltd | Methods of mixing large numbers of heterologous genes |
| US20040067532A1 (en) | 2002-08-12 | 2004-04-08 | Genetastix Corporation | High throughput generation and affinity maturation of humanized antibody |
| KR20080073293A (ko) | 2005-10-14 | 2008-08-08 | 메디뮨 엘엘씨 | 항체 라이브러리의 세포 디스플레이 |
| JP2010517577A (ja) * | 2007-02-09 | 2010-05-27 | メディミューン，エルエルシー | 酵母細胞原形質膜による抗体ライブラリー提示 |
| US8877688B2 (en) | 2007-09-14 | 2014-11-04 | Adimab, Llc | Rationally designed, synthetic antibody libraries and uses therefor |
| EP3124497B1 (de) | 2007-09-14 | 2020-04-15 | Adimab, LLC | Synthetische antikörperbibliotheken mit rationalem design und verwendungen davon |
| US12529164B2 (en) | 2007-09-14 | 2026-01-20 | Adimab, Llc | Rationally designed, synthetic antibody libraries and uses therefor |
| EP2098536A1 (de) | 2008-03-05 | 2009-09-09 | 4-Antibody AG | Isolation und Identifikation antigen- oder ligandenspezifischer Bindungsproteine |
| US8975019B2 (en) * | 2009-10-19 | 2015-03-10 | University Of Massachusetts | Deducing exon connectivity by RNA-templated DNA ligation/sequencing |
| CA2789446A1 (en) | 2010-02-12 | 2011-08-18 | Oncomed Pharmaceuticals, Inc. | Methods for identifying and isolating cells expressing a polypeptide |
| KR102318383B1 (ko) | 2010-07-16 | 2021-10-27 | 아디맵 엘엘씨 | 항체 라이브러리 |
| CA2863224A1 (en) | 2012-01-09 | 2013-07-18 | The Scripps Research Institute | Ultralong complementarity determining regions and uses thereof |
| PT2692865E (pt) * | 2012-07-30 | 2015-02-06 | Nbe Therapeutics Llc | Identificação mediada por transposição de proteínas funcionais ou de ligação específicas |
| EP2943512A4 (de) | 2013-01-11 | 2016-06-01 | California Inst Biomedical Res | Bovine fusionsantikörper |
| AU2015371083B2 (en) * | 2014-12-22 | 2022-01-27 | Universität Zürich | Directed evolution of membrane proteins in eukaryotic cells with a cell wall |
| CN113544275A (zh) * | 2019-03-05 | 2021-10-22 | 信达生物制药(苏州)有限公司 | 展示与分泌目的多肽的酵母展示系统及其用途 |
| CA3165366A1 (en) * | 2020-12-23 | 2022-06-30 | Regeneron Pharmaceuticals, Inc. | Nucleic acids encoding anchor modified antibodies and uses thereof |
| US20240392321A1 (en) * | 2021-09-09 | 2024-11-28 | Bioconsortia, Inc. | Blind editing of polynucleotide sequences |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE229046T1 (de) * | 1985-03-30 | 1987-12-17 | Marc Genf/Geneve Ballivet | Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren. |
| IL84285A (en) * | 1986-10-27 | 1993-03-15 | Int Genetic Engineering | Chimeric antibody with specificity to human tumor antigen |
| US5223409A (en) * | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| ATE414768T1 (de) * | 1991-04-10 | 2008-12-15 | Scripps Research Inst | Bibliotheken heterodimerer rezeptoren mittels phagemiden |
| DE69534347T2 (de) * | 1994-01-31 | 2006-05-24 | Trustees Of Boston University, Boston | Bibliotheken aus Polyklonalen Antikörpern |
| US5643745A (en) * | 1994-02-03 | 1997-07-01 | University Of Hawaii | Heterologous dimeric proteins produced in heterokaryons |
| US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5695941A (en) * | 1994-06-22 | 1997-12-09 | The General Hospital Corporation | Interaction trap systems for analysis of protein networks |
| EP2322614A1 (de) * | 1995-06-07 | 2011-05-18 | Life Technologies Corporation | Rekombinatorische Klonierung unter Verwendung genmanipulierter Rekombinationsorte |
| US6300065B1 (en) * | 1996-05-31 | 2001-10-09 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
| US6461863B1 (en) * | 1996-08-16 | 2002-10-08 | University Of Wyoming | Modifying insect cell gylcosylation pathways with baculovirus expression vectors |
| US5948653A (en) * | 1997-03-21 | 1999-09-07 | Pati; Sushma | Sequence alterations using homologous recombination |
| US6207442B1 (en) * | 1997-10-16 | 2001-03-27 | Zymogenetics, Inc. | Plasmid construction by homologous recombination |
| US6410271B1 (en) * | 2000-06-23 | 2002-06-25 | Genetastix Corporation | Generation of highly diverse library of expression vectors via homologous recombination in yeast |
| US20020165149A1 (en) * | 2000-12-08 | 2002-11-07 | Kranz David M. | Mutated class II major histocompatibility proteins |
-
2003
- 2003-06-16 DE DE60319259T patent/DE60319259T2/de not_active Expired - Lifetime
- 2003-06-16 US US10/462,518 patent/US20030232395A1/en not_active Abandoned
- 2003-06-16 CA CA2489595A patent/CA2489595C/en not_active Expired - Lifetime
- 2003-06-16 AU AU2003240005A patent/AU2003240005B2/en not_active Expired
- 2003-06-16 EP EP03734584A patent/EP1558741B1/de not_active Expired - Lifetime
- 2003-06-16 WO PCT/US2003/018817 patent/WO2003106639A2/en not_active Ceased
- 2003-06-16 AT AT03734584T patent/ATE386810T1/de active
-
2010
- 2010-09-09 AU AU2010219368A patent/AU2010219368B2/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| EP1558741A4 (de) | 2006-02-22 |
| WO2003106639A2 (en) | 2003-12-24 |
| DE60319259D1 (de) | 2008-04-03 |
| AU2003240005A1 (en) | 2003-12-31 |
| DE60319259T2 (de) | 2009-03-05 |
| EP1558741B1 (de) | 2008-02-20 |
| US20030232395A1 (en) | 2003-12-18 |
| CA2489595A1 (en) | 2003-12-24 |
| AU2003240005B2 (en) | 2010-08-12 |
| WO2003106639A3 (en) | 2005-04-21 |
| AU2010219368B2 (en) | 2011-07-28 |
| AU2010219368A1 (en) | 2010-09-30 |
| CA2489595C (en) | 2013-05-28 |
| EP1558741A2 (de) | 2005-08-03 |
| WO2003106639A8 (en) | 2005-06-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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