ATE395435T1 - Verfahren zur sequenzierung von dna - Google Patents

Verfahren zur sequenzierung von dna

Info

Publication number
ATE395435T1
ATE395435T1 AT01963267T AT01963267T ATE395435T1 AT E395435 T1 ATE395435 T1 AT E395435T1 AT 01963267 T AT01963267 T AT 01963267T AT 01963267 T AT01963267 T AT 01963267T AT E395435 T1 ATE395435 T1 AT E395435T1
Authority
AT
Austria
Prior art keywords
nucleotide
base
analogue
target position
incorporated
Prior art date
Application number
AT01963267T
Other languages
English (en)
Inventor
Pal Nyren
Mostafa Ronaghi
Annika Tallsjoe
Original Assignee
Biotage Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotage Ab filed Critical Biotage Ab
Application granted granted Critical
Publication of ATE395435T1 publication Critical patent/ATE395435T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT01963267T 2000-09-07 2001-09-07 Verfahren zur sequenzierung von dna ATE395435T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB0021977.4A GB0021977D0 (en) 2000-09-07 2000-09-07 Method of sequencing DNA

Publications (1)

Publication Number Publication Date
ATE395435T1 true ATE395435T1 (de) 2008-05-15

Family

ID=9899042

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01963267T ATE395435T1 (de) 2000-09-07 2001-09-07 Verfahren zur sequenzierung von dna

Country Status (10)

Country Link
US (2) US7459311B2 (de)
EP (1) EP1317568B1 (de)
JP (1) JP4271439B2 (de)
AT (1) ATE395435T1 (de)
AU (1) AU2001284295A1 (de)
CA (1) CA2421492C (de)
DE (1) DE60134030D1 (de)
ES (1) ES2305099T3 (de)
GB (1) GB0021977D0 (de)
WO (1) WO2002020836A2 (de)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
AU2002227156A1 (en) 2000-12-01 2002-06-11 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
EP1415004A4 (de) 2001-07-03 2005-01-19 Univ Leland Stanford Junior Biolumineszenz regenerierender kreislauf (brc) zur nukleinsäurequantifizierung
US6902921B2 (en) 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US6956114B2 (en) 2001-10-30 2005-10-18 '454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
WO2004029294A1 (en) * 2002-09-27 2004-04-08 Biotage Ab New sequencing method for sequencing rna molecules
GB0300802D0 (en) * 2003-01-14 2003-02-12 Murray James A H Method for detecting DNA polymerisation
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
CA2513899C (en) 2003-01-29 2013-03-26 454 Corporation Methods of amplifying and sequencing nucleic acids
EP1711621A4 (de) 2003-10-28 2008-06-18 Dynal Biotech Llc Primer, verfahren und kits zur amplifikation oder zum nachweis menschlicher leukozytenantigen-allele
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US7635562B2 (en) 2004-05-25 2009-12-22 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
GB0416944D0 (en) * 2004-07-29 2004-09-01 Lumora Ltd Method for determining the amount of template nucleic acid present in a sample
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US20060281100A1 (en) * 2005-06-14 2006-12-14 Shen Gene G Thiotriphosphate nucleotide dye terminators
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US8613889B2 (en) 2006-04-13 2013-12-24 Advanced Liquid Logic, Inc. Droplet-based washing
US8637317B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Method of washing beads
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US8389297B2 (en) * 2006-04-18 2013-03-05 Duke University Droplet-based affinity assay device and system
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US7932034B2 (en) * 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
US20100035252A1 (en) 2008-08-08 2010-02-11 Ion Torrent Systems Incorporated Methods for sequencing individual nucleic acids under tension
US20110311980A1 (en) * 2008-12-15 2011-12-22 Advanced Liquid Logic, Inc. Nucleic Acid Amplification and Sequencing on a Droplet Actuator
US8853373B2 (en) 2009-08-14 2014-10-07 Hitachi, Ltd. Method and reagent for gene sequence analysis
US8916347B2 (en) 2010-02-19 2014-12-23 Agency For Science, Technology And Research Integrated microfluidic and solid state pyrosequencing systems
AU2011226792A1 (en) 2010-06-11 2012-01-12 Life Technologies Corporation Alternative nucleotide flows in sequencing-by-synthesis methods
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US8666678B2 (en) 2010-10-27 2014-03-04 Life Technologies Corporation Predictive model for use in sequencing-by-synthesis
US10273540B2 (en) 2010-10-27 2019-04-30 Life Technologies Corporation Methods and apparatuses for estimating parameters in a predictive model for use in sequencing-by-synthesis
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US20120190020A1 (en) 2011-01-25 2012-07-26 Aria Diagnostics, Inc. Detection of genetic abnormalities
WO2012118745A1 (en) 2011-02-28 2012-09-07 Arnold Oliphant Assay systems for detection of aneuploidy and sex determination
CN103764845B (zh) 2011-04-08 2016-02-17 生命科技股份有限公司 用于合成测序中的相保护试剂流排序
US9513253B2 (en) 2011-07-11 2016-12-06 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based enzymatic assays
EP2732423A4 (de) 2011-07-13 2014-11-26 Multiple Myeloma Res Foundation Inc Verfahren zur erfassung und verteilung von daten
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
EP3937179A1 (de) 2012-05-21 2022-01-12 Distributed Bio Inc Epitopfokussierung durch variable effektive antigenoberflächenkonzentration
EP4148142B1 (de) 2012-05-21 2025-08-13 The Scripps Research Institute Verfahren zur probenvorbereitung
EP2875156A4 (de) 2012-07-19 2016-02-24 Ariosa Diagnostics Inc Sequentielle ligationsbasierte multiplex-detektion von genetischen varianten
US10329608B2 (en) 2012-10-10 2019-06-25 Life Technologies Corporation Methods, systems, and computer readable media for repeat sequencing
US20140296080A1 (en) 2013-03-14 2014-10-02 Life Technologies Corporation Methods, Systems, and Computer Readable Media for Evaluating Variant Likelihood
WO2014200579A1 (en) 2013-06-13 2014-12-18 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US10450562B2 (en) 2014-09-09 2019-10-22 Igenomx International Genomics Corporation Methods and compositions for rapid nucleic acid library preparation
CN104391029B (zh) * 2014-11-17 2016-09-07 中国科学院化学研究所 一种用于测定碱性磷酸酶活性的电化学方法
EP3513280A4 (de) 2017-11-13 2020-07-22 The Multiple Myeloma Research Foundation, Inc. Integrierte, molekulare, omics-, immuntherapeutische, metabolische, epigenetische und klinische datenbank
CN109797195A (zh) * 2019-01-18 2019-05-24 东南大学 基于分析pcr副产物焦磷酸的特异pcr产物快速检测方法及其应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO155316C (no) 1982-04-23 1987-03-11 Sintef Fremgangsmaate for fremstilling av magnetiske polymerpartikler.
US4971903A (en) 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
GB9210168D0 (en) 1992-05-12 1992-06-24 Cemu Bioteknik Ab Method of sequencing dna
GB9311241D0 (en) 1993-06-01 1993-07-21 Celsis Ltd Reagents for use in bioluminescence
GB9620209D0 (en) * 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) * 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
JP2000055917A (ja) 1998-08-05 2000-02-25 Mitsubishi Kagaku Bio-Clinical Laboratories Inc 抗抗原−抗体複合体抗体を用いた検出または測定方法
CN101066447A (zh) 1998-12-22 2007-11-07 微科学有限公司 基因和蛋白质,及它们的用途
GB9901475D0 (en) 1999-01-22 1999-03-17 Pyrosequencing Ab A method of DNA sequencing

Also Published As

Publication number Publication date
EP1317568B1 (de) 2008-05-14
CA2421492A1 (en) 2002-03-14
US20090155801A1 (en) 2009-06-18
CA2421492C (en) 2010-05-04
US20040142330A1 (en) 2004-07-22
WO2002020836A3 (en) 2003-02-06
US7648824B2 (en) 2010-01-19
JP4271439B2 (ja) 2009-06-03
ES2305099T3 (es) 2008-11-01
US7459311B2 (en) 2008-12-02
DE60134030D1 (de) 2008-06-26
AU2001284295A1 (en) 2002-03-22
JP2004508054A (ja) 2004-03-18
GB0021977D0 (en) 2000-10-25
WO2002020836A2 (en) 2002-03-14
EP1317568A2 (de) 2003-06-11

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