ATE397658T1 - Verfahren zur zweidimensionalen konformationsabhängigen trennung nichtzirkulärer nukleinsäuren - Google Patents
Verfahren zur zweidimensionalen konformationsabhängigen trennung nichtzirkulärer nukleinsäurenInfo
- Publication number
- ATE397658T1 ATE397658T1 AT03703010T AT03703010T ATE397658T1 AT E397658 T1 ATE397658 T1 AT E397658T1 AT 03703010 T AT03703010 T AT 03703010T AT 03703010 T AT03703010 T AT 03703010T AT E397658 T1 ATE397658 T1 AT E397658T1
- Authority
- AT
- Austria
- Prior art keywords
- electrophoresis
- nucleic acids
- conformation
- linear nucleic
- sample
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 5
- 238000000926 separation method Methods 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 238000001962 electrophoresis Methods 0.000 abstract 5
- 239000011159 matrix material Substances 0.000 abstract 2
- 239000013043 chemical agent Substances 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000000539 two dimensional gel electrophoresis Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IS6281 | 2002-02-25 | ||
| US36010702P | 2002-02-28 | 2002-02-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE397658T1 true ATE397658T1 (de) | 2008-06-15 |
Family
ID=27758882
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03703010T ATE397658T1 (de) | 2002-02-25 | 2003-02-25 | Verfahren zur zweidimensionalen konformationsabhängigen trennung nichtzirkulärer nukleinsäuren |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7306912B2 (de) |
| EP (1) | EP1476549B1 (de) |
| JP (1) | JP4441267B2 (de) |
| AT (1) | ATE397658T1 (de) |
| AU (1) | AU2003206126A1 (de) |
| CA (1) | CA2477345C (de) |
| DE (1) | DE60321449D1 (de) |
| DK (1) | DK1476549T3 (de) |
| ES (1) | ES2309297T3 (de) |
| WO (1) | WO2003070943A1 (de) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006025074A2 (en) * | 2004-09-02 | 2006-03-09 | Lifeind Ehf. | Twodimensional strandness- and length-dependent separation of nucleic acid fragments |
| US7538113B2 (en) | 2005-02-18 | 2009-05-26 | Wyeth | 4-substituted imidazo[4,5-c]pyridine antagonists of gonadotropin releasing hormone receptor |
| US7582634B2 (en) | 2005-02-18 | 2009-09-01 | Wyeth | 7-substituted imidazo[4,5-c]pyridine antagonists of gonadotropin releasing hormone receptor |
| US7534796B2 (en) | 2005-02-18 | 2009-05-19 | Wyeth | Imidazo[4,5-b]pyridine antagonists of gonadotropin releasing hormone receptor |
| US7531542B2 (en) | 2005-05-18 | 2009-05-12 | Wyeth | Benzooxazole and benzothiazole antagonists of gonadotropin releasing hormone receptor |
| US7582636B2 (en) | 2005-05-26 | 2009-09-01 | Wyeth | Piperazinylimidazopyridine and piperazinyltriazolopyridine antagonists of Gonadotropin Releasing Hormone receptor |
| KR101735133B1 (ko) * | 2009-10-21 | 2017-05-12 | 아사히 가라스 가부시키가이샤 | 임프린트용 몰드, 그 제조 방법, 임프린트 장치 및 임프린트 방법 |
| GB201102385D0 (en) * | 2011-02-10 | 2011-03-30 | Biocule Scotland Ltd | Two-dimensional gel electrophoresis apparatus and method |
| JP6102711B2 (ja) * | 2013-12-10 | 2017-03-29 | 株式会社島津製作所 | 電気泳動分離方法 |
| WO2018015995A1 (ja) * | 2016-07-19 | 2018-01-25 | 株式会社バイオダイナミクス研究所 | 長鎖一本鎖dnaを調製する方法 |
| CN108546735A (zh) * | 2018-04-12 | 2018-09-18 | 大连理工大学 | 一种基于牛磺酸的快速制备核酸电泳缓冲液的预制试粉及其使用方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL9100132A (nl) * | 1991-01-25 | 1992-08-17 | Ingeny Bv | Werkwijze voor het detecteren van dna sequentievariatie. |
| US5874212A (en) * | 1993-05-13 | 1999-02-23 | Thomas Jefferson University | Detection of single base mutations and other variations in double stranded DNA by conformation-sensitive gel electrophoresis |
| US5641626A (en) * | 1993-10-29 | 1997-06-24 | Fmc Corporation | Highly derivatized agarose conformational nucleic acid separation |
| AU2803397A (en) * | 1996-04-17 | 1997-11-07 | Ramot University Authority For Applied Research And Industrial Development Ltd. | Method and kit for detecting genomic instability |
-
2003
- 2003-02-25 EP EP03703010A patent/EP1476549B1/de not_active Expired - Lifetime
- 2003-02-25 AU AU2003206126A patent/AU2003206126A1/en not_active Abandoned
- 2003-02-25 DE DE60321449T patent/DE60321449D1/de not_active Expired - Lifetime
- 2003-02-25 US US10/505,585 patent/US7306912B2/en not_active Expired - Fee Related
- 2003-02-25 JP JP2003569836A patent/JP4441267B2/ja not_active Expired - Fee Related
- 2003-02-25 WO PCT/IS2003/000011 patent/WO2003070943A1/en not_active Ceased
- 2003-02-25 DK DK03703010T patent/DK1476549T3/da active
- 2003-02-25 CA CA2477345A patent/CA2477345C/en not_active Expired - Fee Related
- 2003-02-25 AT AT03703010T patent/ATE397658T1/de not_active IP Right Cessation
- 2003-02-25 ES ES03703010T patent/ES2309297T3/es not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CA2477345C (en) | 2017-05-02 |
| US7306912B2 (en) | 2007-12-11 |
| US20060057575A1 (en) | 2006-03-16 |
| EP1476549A1 (de) | 2004-11-17 |
| ES2309297T3 (es) | 2008-12-16 |
| JP4441267B2 (ja) | 2010-03-31 |
| EP1476549B1 (de) | 2008-06-04 |
| AU2003206126A1 (en) | 2003-09-09 |
| JP2005518202A (ja) | 2005-06-23 |
| CA2477345A1 (en) | 2003-08-28 |
| DK1476549T3 (da) | 2009-06-08 |
| DE60321449D1 (de) | 2008-07-17 |
| WO2003070943A1 (en) | 2003-08-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 1476549 Country of ref document: EP |
|
| REN | Ceased due to non-payment of the annual fee |