ATE399869T1 - Verbesserte bacillus-wirtszelle - Google Patents

Verbesserte bacillus-wirtszelle

Info

Publication number
ATE399869T1
ATE399869T1 AT03709679T AT03709679T ATE399869T1 AT E399869 T1 ATE399869 T1 AT E399869T1 AT 03709679 T AT03709679 T AT 03709679T AT 03709679 T AT03709679 T AT 03709679T AT E399869 T1 ATE399869 T1 AT E399869T1
Authority
AT
Austria
Prior art keywords
host cell
bacillus host
parent
improved bacillus
mutant
Prior art date
Application number
AT03709679T
Other languages
English (en)
Inventor
Jens Andersen
Steen Joergensen
Michael Rasmussen
Peter Olsen
Ib Clausen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE399869T1 publication Critical patent/ATE399869T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/10—Bacillus licheniformis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • External Artificial Organs (AREA)
AT03709679T 2002-04-10 2003-03-25 Verbesserte bacillus-wirtszelle ATE399869T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DKPA200200534 2002-04-10

Publications (1)

Publication Number Publication Date
ATE399869T1 true ATE399869T1 (de) 2008-07-15

Family

ID=29286036

Family Applications (2)

Application Number Title Priority Date Filing Date
AT08159398T ATE522614T1 (de) 2002-04-10 2003-03-25 Mutierte bacillus licheniformis wirtszelle
AT03709679T ATE399869T1 (de) 2002-04-10 2003-03-25 Verbesserte bacillus-wirtszelle

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT08159398T ATE522614T1 (de) 2002-04-10 2003-03-25 Mutierte bacillus licheniformis wirtszelle

Country Status (8)

Country Link
US (3) US7521204B2 (de)
EP (2) EP1995319B1 (de)
CN (1) CN1659283A (de)
AT (2) ATE522614T1 (de)
AU (1) AU2003214034A1 (de)
DE (1) DE60321901D1 (de)
DK (2) DK1995319T3 (de)
WO (1) WO2003093453A2 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100482791C (zh) * 2002-05-14 2009-04-29 诺和酶股份有限公司 果胶酸裂解酶变体
AU2013202674B2 (en) * 2003-07-02 2016-03-03 Bp Corporation North America Inc. Glucanases, nucleic acids encoding them and methods for making and using them
BRPI0412279A (pt) * 2003-07-02 2006-09-19 Diversa Corp glucanases, ácidos nucléicos codificando as mesmas e métodos para preparar e aplicar os mesmos
EP2287179A3 (de) * 2004-01-09 2011-05-18 Novozymes A/S Gesteigerte Bacillus YweA-Expression
EP2305821A3 (de) * 2004-02-13 2011-04-13 Novozymes A/S Proteasevarianten
CN101006174B (zh) 2004-06-21 2010-05-26 诺维信公司 以相同取向稳定保持的至少两个orf的多拷贝
EP1612267A1 (de) * 2004-07-02 2006-01-04 GBF Gesellschaft für Biotechnologische Forschung mbH Zellulasen aus Rumen
DK1863834T3 (da) * 2005-03-22 2010-11-01 Novozymes As Polypeptider og nukleinsyrer, der koder disse
EP2431470B1 (de) * 2006-11-30 2017-11-29 Novozymes A/S Rekombinante wirtszellen der dnase-expression
CA2673176A1 (en) * 2006-12-22 2008-07-03 Dsm Ip Assets B.V. Use of polypeptides as coccidiostat and /or histomonastat
US20120329090A1 (en) * 2009-12-21 2012-12-27 Novozymes A/S Methods for Producing Heterologous Polypeptides in Thiol-Disulfide Oxidoreductase-Deficient Bacterial Mutant Cells
CN109715794A (zh) 2016-07-05 2019-05-03 诺维信公司 果胶裂解酶变体以及编码它们的多核苷酸
CN106467903B (zh) * 2016-09-18 2019-10-08 齐鲁工业大学 一种适用于制浆过程的地衣芽孢杆菌工程菌及其构建方法
CN106893736B (zh) * 2017-01-24 2020-07-07 湖北大学 一种提高地衣芽胞杆菌外源蛋白分泌水平的方法
US11812747B2 (en) 2018-02-06 2023-11-14 Novozymes Bioag A/S Methods of protecting a plant from fungal pests
CN116710471A (zh) 2020-12-15 2023-09-05 诺维信公司 具有减少的细胞运动的突变的宿主细胞
EP4359423A1 (de) 2021-06-24 2024-05-01 Basf Se Bacillus licheniformis-wirtszelle zur herstellung einer verbindung von interesse mit erhöhter reinheit
US20240294894A1 (en) 2021-06-24 2024-09-05 Basf Se Improved bacillus host cell with altered rema/remb protein
EP4387982A2 (de) * 2021-08-20 2024-06-26 Danisco US Inc. Verfahren und zusammensetzungen zur verbesserten proteinproduktion in bacillus-zellen
US20250311734A1 (en) 2022-05-14 2025-10-09 Novozymes A/S Compositions and Methods for Preventing, Treating, Suppressing and/or Eliminatting Phytopathogenic Infestations and Infections
WO2026009000A1 (en) 2024-07-04 2026-01-08 Solasta Bio Limited Methods for production of insecticidal neuropeptides

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CA2003078A1 (en) * 1988-11-18 1990-05-18 Biotechnica International, Inc. Protease deletion
WO1991002803A1 (en) * 1989-08-25 1991-03-07 Henkel Research Corporation A method of suppressing chromosomal gene expression
DK639689D0 (da) 1989-12-18 1989-12-18 Novo Nordisk As Indfoering af dna i celler
US5294542A (en) * 1991-03-19 1994-03-15 Omnigene, Inc. Residual protease-III
EP0667910B1 (de) 1991-11-14 2003-08-27 Novozymes A/S Ein bacillus-promotor, der von dem alpha-amylase-promotor einer variante des bacillus licheniformis abstammt
DK153992D0 (da) 1992-12-22 1992-12-22 Novo Nordisk As Metode
GB2279955B (en) * 1993-07-15 1998-02-18 Solvay Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof
JP3649338B2 (ja) 1994-06-03 2005-05-18 ノボザイムス バイオテック，インコーポレイティド 精製されたマイセリオフトラ・ラッカーゼ及びそれをコードする核酸
EP0805867B1 (de) 1995-01-23 2003-12-17 Novozymes A/S Dna-integration durch transposition
US7199233B1 (en) 1996-08-23 2007-04-03 Peter Ruhdal Jensen Artificial promoter libraries for selected organisms and promoters derived from such libraries
GB9708452D0 (en) * 1997-04-26 1997-06-18 Univ Newcastle Improved prokaryotic expression of protein
DE69916812T2 (de) 1998-02-12 2005-04-14 Novozymes A/S Prokaryontische zelle, die zwei kopien eines genes enthält, die in zwei verschiedene richtungen transkribiert werden.
US6100063A (en) 1998-02-12 2000-08-08 Novo Nordisk A/S Procaryotic cell comprising at least two copies of a gene
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
WO1999045124A2 (en) * 1998-03-04 1999-09-10 Genencor International, Inc. Modified forms of pullulanase
AU2001262063A1 (en) 2000-05-24 2001-12-03 Novozymes A/S Method for increasing gene copy number in a host cell and resulting host cell
JP2004501651A (ja) 2000-06-23 2004-01-22 ノボザイムス　アクティーゼルスカブ 遺伝子の安定した染色体多コピー組み込みのための方法
US7018794B2 (en) * 2000-10-06 2006-03-28 Novozymes, Inc. Methods for monitoring multiple gene expression

Also Published As

Publication number Publication date
DK1995319T3 (da) 2011-12-05
DE60321901D1 (de) 2008-08-14
EP1497429B1 (de) 2008-07-02
WO2003093453A3 (en) 2004-05-13
DK1497429T3 (da) 2008-11-03
AU2003214034A1 (en) 2003-11-17
US20090176290A1 (en) 2009-07-09
US20050244922A1 (en) 2005-11-03
US7521204B2 (en) 2009-04-21
EP1995319A1 (de) 2008-11-26
WO2003093453A2 (en) 2003-11-13
ATE522614T1 (de) 2011-09-15
CN1659283A (zh) 2005-08-24
EP1497429A2 (de) 2005-01-19
EP1995319B1 (de) 2011-08-31
US8911969B2 (en) 2014-12-16
US20090203074A1 (en) 2009-08-13
AU2003214034A8 (en) 2003-11-17

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