ATE402997T1 - Mutierte penicillin g acylasen - Google Patents

Mutierte penicillin g acylasen

Info

Publication number
ATE402997T1
ATE402997T1 AT97947397T AT97947397T ATE402997T1 AT E402997 T1 ATE402997 T1 AT E402997T1 AT 97947397 T AT97947397 T AT 97947397T AT 97947397 T AT97947397 T AT 97947397T AT E402997 T1 ATE402997 T1 AT E402997T1
Authority
AT
Austria
Prior art keywords
penicillin
acylases
acylase
amino acid
mutated penicillin
Prior art date
Application number
AT97947397T
Other languages
English (en)
Inventor
Li You
John Usher
Brenda White
Jiri Novotny
Original Assignee
Bristol Myers Squibb Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bristol Myers Squibb Co filed Critical Bristol Myers Squibb Co
Application granted granted Critical
Publication of ATE402997T1 publication Critical patent/ATE402997T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Inorganic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AT97947397T 1996-11-05 1997-10-31 Mutierte penicillin g acylasen ATE402997T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US3036596P 1996-11-05 1996-11-05

Publications (1)

Publication Number Publication Date
ATE402997T1 true ATE402997T1 (de) 2008-08-15

Family

ID=21853878

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97947397T ATE402997T1 (de) 1996-11-05 1997-10-31 Mutierte penicillin g acylasen

Country Status (11)

Country Link
US (1) US6403356B1 (de)
EP (1) EP0961825B1 (de)
JP (1) JP4080542B2 (de)
KR (1) KR100570244B1 (de)
AT (1) ATE402997T1 (de)
AU (1) AU718648B2 (de)
CA (1) CA2270400C (de)
DE (1) DE69738873D1 (de)
HU (1) HU226348B1 (de)
IL (1) IL129055A (de)
WO (1) WO1998020120A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2144139T3 (es) * 1994-08-12 2000-06-01 Dsm Nv Genes de la penicilina g-acilasa mutados.
ATE402997T1 (de) 1996-11-05 2008-08-15 Bristol Myers Squibb Co Mutierte penicillin g acylasen
AU4436900A (en) * 1999-04-29 2000-11-17 Dsm N.V. Expression cassette for efficient production of a protein
KR20040075042A (ko) * 2001-12-27 2004-08-26 디에스엠 아이피 어셋츠 비.브이. β-락탐 항생제의 제조 방법
ITMI20030042A1 (it) * 2003-01-14 2004-07-15 Univ Degli Studi Trieste Processo di sintesi enzimatica di antibiotici b lattamici.
KR100530299B1 (ko) * 2003-08-11 2005-11-22 산도즈 게엠베하 변이 세팔로스포린 ｃ 아실라제 및 이를 이용한 7-ａｃａ 제조방법
WO2006069984A2 (en) * 2004-12-27 2006-07-06 Dsm Ip Assets B.V. Process for the synthesis of cefaclor
CN101177688B (zh) * 2006-11-08 2010-12-01 中国科学院上海生命科学研究院 突变青霉素g酰化酶、其重组表达质粒及转化的工程菌株
CN101802212B (zh) 2007-07-27 2013-08-28 酵素生物技术有限公司 从大肠杆菌BL21CCM7394中无色菌CCM4824制备固定重组青霉素酰化酶催化剂的方法及其在合成β-内酰胺抗生素中的应用
HUE036302T2 (hu) 2008-12-23 2018-07-30 Dsm Sinochem Pharm Nl Bv Mutáns penicillin-G-acilázok
CN102656274B (zh) 2009-12-14 2014-10-15 中化帝斯曼制药有限公司荷兰公司 生产头孢拉定的方法
WO2011113486A1 (en) 2010-03-17 2011-09-22 Deretil, S.A. Process for the synthesis of hydroxyphenylglycine esters
RU2564578C2 (ru) * 2012-04-25 2015-10-10 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) МУТАНТ ПЕНИЦИЛЛИНАЦИЛАЗЫ ИЗ E.coli С УЛУЧШЕННЫМИ СВОЙСТВАМИ
RU2537845C2 (ru) * 2012-04-25 2015-01-10 Федеральное государственное бюджетное образовательное учреждение высшего профессиональногообразования "Московский государственный университет имени М.В. Ломоносова " (МГУ) Способ синтеза пептидов, в том числе бета-лактамных антибиотиков, при использовании варианта пенициллинацилазы
RU2576002C2 (ru) * 2013-10-18 2016-02-27 Российская Федерация, От Имени Которой Выступает Министерство Образования И Науки Российской Федерации Способ улучшения каталитических свойств пенициллинацилазы из escherichia coli и применение мутантной пенициллинацилазы
CN103667418B (zh) * 2013-12-18 2015-03-25 华北制药河北华民药业有限责任公司 高活性β-内酰胺抗生素合成用酶的高通量筛选方法
WO2017186864A1 (en) 2016-04-27 2017-11-02 Sandoz Ag Enzymatic process for the production of beta-lactam antibiotics in the presence of particulate inoculum
WO2017193022A1 (en) * 2016-05-05 2017-11-09 Codexis, Inc. Penicillin-g acylases
KR101985911B1 (ko) * 2017-12-28 2019-06-04 아미코젠주식회사 Achromobacter sp. CCM 4824 유래 페니실린 G 아실라제 변이체 및 이의 이용
KR102363768B1 (ko) * 2019-11-15 2022-02-16 아미코젠주식회사 세파졸린 생산성이 증가된 페니실린 g 아실라제 변이체 및 이의 이용
CN115418331B (zh) * 2022-08-29 2024-09-10 新发药业有限公司 一种产青霉素g酰基转移酶自诱导培养基及其应用
CN119242757B (zh) 2024-10-29 2025-05-16 浙江师范大学行知学院 一种从酶催化一步法制备阿莫西林的反应液中分离阿莫西林和苯乙酸的方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554250A (en) * 1983-06-30 1985-11-19 E. R. Squibb & Sons, Inc. Method of increased production of penicillin acylase and plasmid employed therein
JPS6121097A (ja) 1984-07-10 1986-01-29 Asahi Chem Ind Co Ltd ７−アミノセフアロスポラン酸及びその誘導体の製造法
SE8403929L (sv) 1984-07-31 1986-02-21 Sten Gatenbeck Rekombinant-dna-molekyl, transformerade mikroorganismer och forfarande for framstellning av penicillin v-amidas
DE3439843A1 (de) * 1984-10-31 1986-04-30 Boehringer Mannheim Gmbh, 6800 Mannheim Plasmide zur erhoehten produktion von penicillin g-amidase
US4981789A (en) 1987-03-18 1991-01-01 Merck & Co., Inc. One-step enzymatic conversion of cephalosporin C and derivatives to 7-aminocephalosporanic acid and derivatives
EP0322032A3 (de) 1987-12-21 1991-01-30 Merck & Co. Inc. Enzymatische Umwandlung von Cephalosporin C und Derivaten in 7-Aminocephalosporinsäure und Derivate in einem Schritt
US5229274A (en) * 1989-06-27 1993-07-20 Merck & Co., Inc. Gene encoding one step cephalosporin C amidase and expression thereof in recombinant bacillus
KR0152667B1 (ko) 1990-04-18 1998-10-01 한스 월터 라벤 페니실린 g 아실라아제, 이 효소를 코드화한 유전자 및 이 효소의 제조방법
ES2119756T3 (es) 1990-04-18 1998-10-16 Gist Brocades Nv Genes de beta lactama acilasa mutados.
GB9019724D0 (en) 1990-09-10 1990-10-24 Fujisawa Pharmaceutical Co Cephalosporin c acylase
GB9204439D0 (en) 1992-02-27 1992-04-15 Fujisawa Pharmaceutical Co A new cephalosporin c acylase
TW400384B (en) 1993-11-01 2000-08-01 Fujisawa Pharmaceutical Co A new cephalosporin C acylase
ES2144139T3 (es) * 1994-08-12 2000-06-01 Dsm Nv Genes de la penicilina g-acilasa mutados.
US5516679A (en) 1994-12-23 1996-05-14 Bristol-Myers Squibb Company Penicillin V amidohydrolase gene from Fusarium oxysporum
ATE402997T1 (de) 1996-11-05 2008-08-15 Bristol Myers Squibb Co Mutierte penicillin g acylasen

Also Published As

Publication number Publication date
CA2270400A1 (en) 1998-05-14
JP4080542B2 (ja) 2008-04-23
US6403356B1 (en) 2002-06-11
HU226348B1 (en) 2008-09-29
WO1998020120A1 (en) 1998-05-14
EP0961825B1 (de) 2008-07-30
DE69738873D1 (de) 2008-09-11
IL129055A (en) 2005-06-19
HK1024933A1 (en) 2000-10-27
AU718648B2 (en) 2000-04-20
AU5248698A (en) 1998-05-29
KR100570244B1 (ko) 2006-04-12
IL129055A0 (en) 2000-02-17
HUP9904162A2 (hu) 2000-03-28
JP2001503272A (ja) 2001-03-13
CA2270400C (en) 2010-09-28
EP0961825A4 (de) 2002-11-20
HUP9904162A3 (en) 2001-06-28
EP0961825A1 (de) 1999-12-08
KR20000053041A (ko) 2000-08-25

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