ATE400655T1 - Polynucleotidsynthese mittels prozessierendem enzym - Google Patents

Polynucleotidsynthese mittels prozessierendem enzym

Info

Publication number
ATE400655T1
ATE400655T1 AT00919020T AT00919020T ATE400655T1 AT E400655 T1 ATE400655 T1 AT E400655T1 AT 00919020 T AT00919020 T AT 00919020T AT 00919020 T AT00919020 T AT 00919020T AT E400655 T1 ATE400655 T1 AT E400655T1
Authority
AT
Austria
Prior art keywords
polynucleotide synthesis
enzyme
processing enzyme
polynucleotide
nucleotide
Prior art date
Application number
AT00919020T
Other languages
English (en)
Inventor
Daniel Henry Densham
Original Assignee
Medical Biosystems Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Biosystems Ltd filed Critical Medical Biosystems Ltd
Application granted granted Critical
Publication of ATE400655T1 publication Critical patent/ATE400655T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/215—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Halobacteriaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Low-Molecular Organic Synthesis Reactions Using Catalysts (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Saccharide Compounds (AREA)
AT00919020T 1999-04-06 2000-04-06 Polynucleotidsynthese mittels prozessierendem enzym ATE400655T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB9907813.1A GB9907813D0 (en) 1999-04-06 1999-04-06 Synthesis

Publications (1)

Publication Number Publication Date
ATE400655T1 true ATE400655T1 (de) 2008-07-15

Family

ID=10851008

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00919020T ATE400655T1 (de) 1999-04-06 2000-04-06 Polynucleotidsynthese mittels prozessierendem enzym

Country Status (17)

Country Link
US (1) US6623929B1 (de)
EP (1) EP1165786B1 (de)
JP (1) JP2002540788A (de)
KR (1) KR100671070B1 (de)
CN (1) CN1161466C (de)
AT (1) ATE400655T1 (de)
AU (1) AU774308B2 (de)
BR (1) BR0009530A (de)
CA (1) CA2367269A1 (de)
DE (1) DE60039421D1 (de)
GB (1) GB9907813D0 (de)
HK (1) HK1041285B (de)
IL (1) IL145578A0 (de)
IS (1) IS6088A (de)
MX (1) MXPA01009998A (de)
NZ (1) NZ514346A (de)
WO (1) WO2000060072A1 (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998035012A2 (en) 1997-02-12 1998-08-13 Chan Eugene Y Methods and products for analyzing polymers
ATE225858T1 (de) * 1997-07-28 2002-10-15 Medical Biosystems Ltd Sequenzanalyse von nukleinsäuren
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6927065B2 (en) 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
EP1354064A2 (de) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatische nukleinsäuresynthese: zusammensetzungen und verfahren, um die zuverlässigkeit des monomereinbaus zu erhöhen
US6956114B2 (en) 2001-10-30 2005-10-18 '454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US6902921B2 (en) 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
AU2004209001B2 (en) 2003-01-29 2007-10-11 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US20040203084A1 (en) * 2003-04-10 2004-10-14 Doug Levinson Profiling conformational variants, antibody compositions and methods of using the same
JP2005161480A (ja) * 2003-12-03 2005-06-23 Canon Inc 物体処理装置、物体の移動方法及びその移動方法を用いて処理された物体
EP1704256A4 (de) 2004-01-13 2008-01-16 Us Genomics Inc Nachweis und quantifizierung von analyten in lösung unter verwendung von polymeren
US7595160B2 (en) 2004-01-13 2009-09-29 U.S. Genomics, Inc. Analyte detection using barcoded polymers
CA2569597A1 (en) * 2004-06-07 2005-12-22 California Institute Of Technology Detection of dna mismatches and oxidative lesions
EP1790202A4 (de) 2004-09-17 2013-02-20 Pacific Biosciences California Vorrichtung und verfahren zur analyse von molekülen
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
EP3045542B1 (de) 2008-03-28 2016-11-16 Pacific Biosciences of California, Inc. Verfahren zur nukleinsäuresequenzierung
FR3020071B1 (fr) * 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
WO2017109077A1 (en) * 2015-12-22 2017-06-29 Roche Diagnostics Gmbh Compositions with thermolabile dyes
EP3665274B1 (de) 2017-08-07 2025-10-15 DNA Script Varianten der familie-a-dna-polymerase und verwendungen davon
US10752887B2 (en) 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
WO2019135007A1 (en) 2018-01-08 2019-07-11 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
EP3826762A1 (de) 2018-07-23 2021-06-02 DNA Script Massiv parallele enzymatische synthese von nukleinsäuresträngen
CN113423841A (zh) 2018-12-13 2021-09-21 Dna斯克瑞普特公司 细胞和生物分子上的直接寡核苷酸合成
EP3924500A1 (de) 2019-02-12 2021-12-22 DNA Script Effiziente produktspaltung in der template-freien enzymatischen synthese von polynukleotiden

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE462454B (sv) 1988-11-10 1990-06-25 Pharmacia Ab Maetyta foer anvaendning i biosensorer
DE4406524A1 (de) * 1994-02-28 1995-08-31 Boehringer Mannheim Gmbh 3'-RNA-Markierung mit Terminaler Transferase
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
ATE143057T1 (de) 1994-10-17 1996-10-15 Harvard College Dns polymerase mit veränderter nukleotid- bindungstelle
WO1997009451A1 (en) 1995-09-08 1997-03-13 Life Technologies, Inc. Cloned dna polymerases from thermotoga and mutants thereof
DE19601385A1 (de) * 1996-01-16 1997-07-17 Mueller Manfred W Verfahren zur Amplifikation von Nukleinsäure
DE69806003T2 (de) 1997-03-12 2003-01-30 Pe Corp. (Ny), Foster City Dna polymerasen, die verbesserte fähigkeiten zum einbau markierter nukleotide besitzen
ATE225858T1 (de) * 1997-07-28 2002-10-15 Medical Biosystems Ltd Sequenzanalyse von nukleinsäuren
WO1999010366A1 (en) 1997-08-29 1999-03-04 Life Technologies, Inc. High fidelity polymerases and uses thereof

Also Published As

Publication number Publication date
EP1165786B1 (de) 2008-07-09
AU774308B2 (en) 2004-06-24
KR100671070B1 (ko) 2007-01-17
MXPA01009998A (es) 2002-08-20
AU3978000A (en) 2000-10-23
HK1041285A1 (en) 2002-07-05
NZ514346A (en) 2003-03-28
BR0009530A (pt) 2002-02-05
US6623929B1 (en) 2003-09-23
CN1348494A (zh) 2002-05-08
GB9907813D0 (en) 1999-06-02
IL145578A0 (en) 2002-06-30
JP2002540788A (ja) 2002-12-03
IS6088A (is) 2001-09-26
DE60039421D1 (de) 2008-08-21
CA2367269A1 (en) 2000-10-12
KR20010111579A (ko) 2001-12-19
WO2000060072A1 (en) 2000-10-12
CN1161466C (zh) 2004-08-11
HK1041285B (en) 2009-04-09
EP1165786A1 (de) 2002-01-02

Similar Documents

Publication Publication Date Title
ATE400655T1 (de) Polynucleotidsynthese mittels prozessierendem enzym
EP1456409A4 (de) Paralleles scoring von polymorphismen mittels amplifikation und fehlerkorrektur
ATE346938T1 (de) Polyketid synthase enzyme und rekombinante dna konstrukte dafür, zur herstellung von mit fk-506 und fk-520 verwandten verbindungen
NO20011174D0 (no) Nye heteroaromatiske inhibitorer av fruktose-1,6-bisfosfotase
IS7638A (is) Asabísýkló-oktan- og nónanafleiður með DDP-IV tálmunarvirkni
IT1165700B (it) Procedimenti enzimatici
BR0313396A (pt) 3-pirrolil-piridopirazóis e 3-pirrolil-indazóis, como inibidores de quinase
DE50014190D1 (de) Verwendung von phthalazin-derivaten zur behandlung neurodegenerativer krankheiten
NZ515673A (en) Compositions and methods for labeling of nucleic acid molecules
NZ514347A (en) Polynucleotide sequencing detecting the interaction between the polynucleotide and a helicase / primase enzyme by measuring radiation
EA200701742A1 (ru) Способы получения сиропа
EP2113563A3 (de) Varianten eines lipolytischen Enzyms
DE3573135D1 (en) Method for purifying reaction solution obtained by using microbial cell, immobilized microbial cell, or immobilized enzyme
DE3751280D1 (de) Enzyme als landwirtschaftliche chemische Hilfsmittel.
ATE84313T1 (de) Substratunterstuetzte katalyse.
DE60332600D1 (de) Selektive elution von immobilisierten multiplexierten primerverlängerungsprodukten
DE69923676D1 (de) Bestimmte heteroaryl substituierte thiol inhibitoren von endothelin wechselnden enzymen
ATE238673T1 (de) Galactoseoxidase enthaltende zusammensetzung und ihre verwendung
WO2000065098A3 (en) Nucleotide extension on a microarray of gel-immobilized primers
DK1238057T3 (da) Fremgangsmåde til produktion af vedhæftende dyreceller
BRPI0515397B8 (pt) métodos para detecção e identificação específicas de streptococcus agalactiae usando atividade de esterase, e meio de reação
ATE334209T1 (de) Inaktivierung von genen des mep-wegs
ATE331729T1 (de) Tetrapeptidische tryptase substrate und ihre verwendung zur tryptase aktivitätbestimmung
GB2033428B (en) Enzymatic processes
ATE361982T1 (de) Enon reduktase gen und mikrobielle herstellung von levodion

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties