ATE400660T1 - Vektoren für genmutagenese und genentdeckung - Google Patents

Vektoren für genmutagenese und genentdeckung

Info

Publication number
ATE400660T1
ATE400660T1 AT99914126T AT99914126T ATE400660T1 AT E400660 T1 ATE400660 T1 AT E400660T1 AT 99914126 T AT99914126 T AT 99914126T AT 99914126 T AT99914126 T AT 99914126T AT E400660 T1 ATE400660 T1 AT E400660T1
Authority
AT
Austria
Prior art keywords
gene
vectors
discovery
mutagenesis
novel
Prior art date
Application number
AT99914126T
Other languages
English (en)
Inventor
Brian Zambrowicz
Glenn Friedrich
Arthur Sands
Original Assignee
Lexicon Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/057,328 external-priority patent/US6080576A/en
Application filed by Lexicon Pharmaceuticals Inc filed Critical Lexicon Pharmaceuticals Inc
Application granted granted Critical
Publication of ATE400660T1 publication Critical patent/ATE400660T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/02Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1037Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1051Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/16011Human Immunodeficiency Virus, HIV
    • C12N2740/16041Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00Nucleic acids vectors
    • C12N2800/60Vectors containing traps for, e.g. exons, promoters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00Vectors comprising a special translation-regulating system
    • C12N2840/20Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00Vectors comprising a special translation-regulating system
    • C12N2840/44Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT99914126T 1998-03-27 1999-03-26 Vektoren für genmutagenese und genentdeckung ATE400660T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7972998P 1998-03-27 1998-03-27
US09/057,328 US6080576A (en) 1998-03-27 1998-04-08 Vectors for gene trapping and gene activation
US8172798P 1998-04-14 1998-04-14

Publications (1)

Publication Number Publication Date
ATE400660T1 true ATE400660T1 (de) 2008-07-15

Family

ID=27369230

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99914126T ATE400660T1 (de) 1998-03-27 1999-03-26 Vektoren für genmutagenese und genentdeckung

Country Status (8)

Country Link
EP (1) EP1066392B1 (de)
JP (1) JP3725782B2 (de)
AT (1) ATE400660T1 (de)
AU (1) AU751520B2 (de)
CA (1) CA2323834C (de)
DE (1) DE69939056D1 (de)
DK (1) DK1066392T3 (de)
WO (1) WO1999050426A1 (de)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7332338B2 (en) 1996-10-04 2008-02-19 Lexicon Pharmaceuticals, Inc. Vectors for making genomic modifications
US6740503B1 (en) 1997-09-26 2004-05-25 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
AU772677B2 (en) * 1998-03-27 2004-05-06 Lexicon Pharmaceuticals, Inc. Vectors for gene mutagenesis and gene discovery
US6436707B1 (en) 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6808921B1 (en) 1998-03-27 2004-10-26 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
AU2004203361B2 (en) * 1998-03-27 2007-05-10 Lexicon Pharmaceuticals, Inc. Vectors for gene mutagenesis and gene discovery by gene trapping
AU5470399A (en) 1998-08-10 2000-03-06 Lexicon Genetics Incorporated Construction of normalized cdna libraries from eucaryotic cells
EP1584689A1 (de) * 1998-11-20 2005-10-12 Lexicon Genetics Incorporated Vektoren für die Gen-Mutagenese und die Entdeckung von Genen
WO2000049162A2 (en) * 1999-02-19 2000-08-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
ATE460492T1 (de) 1999-07-14 2010-03-15 Transgenic Inc ßGEN-FALLEN-VEKTORß UND VERFAHREN ZUM ßEINFANGENß VON GENEN MITTELS DIESES VEKTORS
EP1092768A1 (de) * 1999-10-16 2001-04-18 ARTEMIS Pharmaceuticals GmbH Konstrukt zum bedingten einfangen und Disruption von Genen
US6897020B2 (en) 2000-03-20 2005-05-24 Newlink Genetics Inc. Methods and compositions for elucidating relative protein expression levels in cells
CA2403567A1 (en) * 2000-03-20 2001-09-27 Newlink Genetics Methods and compositions for elucidating protein expression profiles in cells
GB0018876D0 (en) 2000-08-01 2000-09-20 Applied Research Systems Method of producing polypeptides
US7449179B2 (en) 2000-11-16 2008-11-11 Cornell Research Foundation, Inc. Vectors for conditional gene inactivation
US20020094536A1 (en) * 2000-12-28 2002-07-18 Cell Therapeutics, Inc. Methods for making polynucleotide libraries, polynucleotide arrays, and cell libraries for high-throughput genomics analysis
ATE406094T1 (de) * 2001-03-28 2008-09-15 Dana Farber Cancer Inst Inc Identifizierung und charakterisierung von genen
MXPA04001725A (es) 2001-08-24 2005-04-11 Advanced Cell Tech Inc ANáLISIS DE SELECCION PARA IDENTIFICAR AGENTES INDUCTORES DE DIFERENCIACION Y PRODUCCION DE CELULAS DIFERENCIADAS PARA TERAPIA CELULAR.
US20040091936A1 (en) 2002-05-24 2004-05-13 Michael West Bank of stem cells for producing cells for transplantation having HLA antigens matching those of transplant recipients, and methods for making and using such a stem cell bank
US7205148B2 (en) * 2003-06-11 2007-04-17 Regeneron Pharmaceuticals, Inc. Genome mutation by intron insertion into an embryonic stem cell genome
KR100747381B1 (ko) * 2006-01-20 2007-08-07 미쓰비시덴키 가부시키가이샤 엘리베이터의 그룹 관리 제어 장치
JP4867012B2 (ja) * 2007-03-22 2012-02-01 国立大学法人群馬大学 レトロウイルス産生用ベクター
EP2318527A4 (de) * 2008-07-30 2012-07-04 Michael Strathmann Gen-targeting-verfahren
CN112824529B (zh) * 2019-11-21 2022-09-09 北京大学 一种条件性基因敲除或者拯救方法
US20230287458A1 (en) * 2020-07-14 2023-09-14 Abeona Therapeutics Inc. Recombinant adeno-associated viral vectors for multipartite gene delivery

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0920508A1 (de) * 1996-08-23 1999-06-09 Chiron Corporation Menschliches polyhomeotisch 1 (hph1) wirkt wie ein oncogen
US6207371B1 (en) * 1996-10-04 2001-03-27 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same

Also Published As

Publication number Publication date
WO1999050426A1 (en) 1999-10-07
EP1066392A1 (de) 2001-01-10
JP2002509727A (ja) 2002-04-02
CA2323834C (en) 2009-01-27
AU3203699A (en) 1999-10-18
AU751520B2 (en) 2002-08-15
DK1066392T3 (da) 2008-08-25
DE69939056D1 (de) 2008-08-21
EP1066392B1 (de) 2008-07-09
CA2323834A1 (en) 1999-10-07
JP3725782B2 (ja) 2005-12-14

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