ATE405676T1 - Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination - Google Patents
Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombinationInfo
- Publication number
- ATE405676T1 ATE405676T1 AT03717952T AT03717952T ATE405676T1 AT E405676 T1 ATE405676 T1 AT E405676T1 AT 03717952 T AT03717952 T AT 03717952T AT 03717952 T AT03717952 T AT 03717952T AT E405676 T1 ATE405676 T1 AT E405676T1
- Authority
- AT
- Austria
- Prior art keywords
- double stranded
- methods
- recombination
- complexes
- stranded dna
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 6
- 108020004414 DNA Proteins 0.000 title 1
- 102000053602 DNA Human genes 0.000 title 1
- 238000005215 recombination Methods 0.000 title 1
- 230000006798 recombination Effects 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36372902P | 2002-03-11 | 2002-03-11 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE405676T1 true ATE405676T1 (de) | 2008-09-15 |
Family
ID=28041805
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03717952T ATE405676T1 (de) | 2002-03-11 | 2003-03-11 | Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7176025B2 (de) |
| EP (1) | EP1488001B1 (de) |
| JP (1) | JP2005519621A (de) |
| AT (1) | ATE405676T1 (de) |
| AU (1) | AU2003222269A1 (de) |
| CA (1) | CA2478875A1 (de) |
| DE (1) | DE60323067D1 (de) |
| WO (1) | WO2003078645A2 (de) |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
| US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
| AU2002228974A1 (en) | 2000-12-13 | 2002-06-24 | Nugen Technologies, Inc | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
| US6946251B2 (en) | 2001-03-09 | 2005-09-20 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences using RNA-DNA composite primers |
| CA2439074A1 (en) | 2001-03-09 | 2002-09-19 | Nugen Technologies, Inc. | Methods and compositions for amplification of rna sequences |
| US7176025B2 (en) | 2002-03-11 | 2007-02-13 | Nugen Technologies, Inc. | Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination |
| EP1573056A4 (de) * | 2002-05-17 | 2007-11-28 | Nugen Technologies Inc | Verfahren zur fragmentierung, markierung und immobilisierung von nukleinsäuren |
| WO2004092418A2 (en) | 2003-04-14 | 2004-10-28 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
| WO2005065321A2 (en) * | 2003-12-29 | 2005-07-21 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
| JP4772055B2 (ja) * | 2004-10-20 | 2011-09-14 | 日立化成工業株式会社 | ｍＲＮＡの定量により薬剤の投与を選定する方法 |
| EP1929046B1 (de) * | 2005-09-07 | 2012-07-11 | Nugen Technologies, Inc. | Verbessertes nukleinsäureamplifikationsverfahren |
| US20090275486A1 (en) * | 2006-05-16 | 2009-11-05 | Nurith Kurn | Nucleic acid separation and purification method based on reversible charge interactions |
| US20100022403A1 (en) * | 2006-06-30 | 2010-01-28 | Nurith Kurn | Methods for fragmentation and labeling of nucleic acids |
| US20090203531A1 (en) * | 2008-02-12 | 2009-08-13 | Nurith Kurn | Method for Archiving and Clonal Expansion |
| WO2009117698A2 (en) | 2008-03-21 | 2009-09-24 | Nugen Technologies, Inc. | Methods of rna amplification in the presence of dna |
| US20110189679A1 (en) * | 2009-09-11 | 2011-08-04 | Nugen Technologies, Inc. | Compositions and methods for whole transcriptome analysis |
| SG11201401628WA (en) | 2011-10-19 | 2014-05-29 | Nugen Technologies Inc | Compositions and methods for directional nucleic acid amplification and sequencing |
| SG10201504490QA (en) | 2012-01-26 | 2015-07-30 | Nugen Technologies Inc | Compositions And Methods For Targeted Nucleic Acid Sequence Enrichment And High Efficiency Library Generation |
| US9487828B2 (en) | 2012-05-10 | 2016-11-08 | The General Hospital Corporation | Methods for determining a nucleotide sequence contiguous to a known target nucleotide sequence |
| US9957549B2 (en) | 2012-06-18 | 2018-05-01 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| WO2014028311A2 (en) * | 2012-08-15 | 2014-02-20 | President And Fellows Of Harvard College | Polynucleotide-binding domains as a means of cell labeling, cell organization and polymer sequencing |
| US20140274738A1 (en) | 2013-03-15 | 2014-09-18 | Nugen Technologies, Inc. | Sequential sequencing |
| WO2015073711A1 (en) | 2013-11-13 | 2015-05-21 | Nugen Technologies, Inc. | Compositions and methods for identification of a duplicate sequencing read |
| EP4219744A3 (de) | 2014-01-27 | 2023-08-30 | The General Hospital Corporation | Verfahren zur herstellung von nukleinsäuren zur sequenzierung |
| WO2015131107A1 (en) | 2014-02-28 | 2015-09-03 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
| JP6803327B2 (ja) | 2014-08-06 | 2020-12-23 | ニューゲン テクノロジーズ， インコーポレイテッド | 標的化されたシークエンシングからのデジタル測定値 |
| WO2018053362A1 (en) | 2016-09-15 | 2018-03-22 | ArcherDX, Inc. | Methods of nucleic acid sample preparation |
| WO2018053365A1 (en) | 2016-09-15 | 2018-03-22 | ArcherDX, Inc. | Methods of nucleic acid sample preparation for analysis of cell-free dna |
| US12492430B2 (en) | 2017-04-11 | 2025-12-09 | Tecan Genomics, Inc. | Library quantitation and qualification |
| US20200263233A1 (en) * | 2017-06-23 | 2020-08-20 | Eiken Kagaku Kabushiki Kaisha | Method For Nucleic Acid Detection, Primer For Nucleic Acid Detection, And Kit For Nucleic Acid Detection |
| US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
| US12059674B2 (en) | 2020-02-03 | 2024-08-13 | Tecan Genomics, Inc. | Reagent storage system |
Family Cites Families (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4876187A (en) * | 1985-12-05 | 1989-10-24 | Meiogenics, Inc. | Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences |
| US5403711A (en) * | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
| JP2650159B2 (ja) * | 1988-02-24 | 1997-09-03 | アクゾ・ノベル・エヌ・ベー | 核酸増幅方法 |
| JP2856804B2 (ja) | 1988-03-24 | 1999-02-10 | ユニバーシティ オブ アイオワ リサーチ ファウンデイション | 標識された相補的核酸プローブを開裂する触媒反応の補因子としての活性に基づく核酸配列検出のための触媒ハイブリッド形成系 |
| US5130238A (en) * | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
| CA2020958C (en) * | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| EP0731175A3 (de) * | 1989-07-11 | 2004-05-26 | Gen-Probe Incorporated | Oligonukleotid-Sonden für Nukleinsäure von HIV |
| CA2037349C (en) * | 1990-03-26 | 2008-06-17 | James G. Wetmur | Branch migration of nucleotides |
| HU218095B (hu) * | 1990-05-01 | 2000-05-28 | Amgen Inc. | Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban |
| US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| AU8997991A (en) | 1991-01-31 | 1992-08-06 | Becton Dickinson & Company | Exonuclease mediated strand displacement amplification |
| US5169766A (en) * | 1991-06-14 | 1992-12-08 | Life Technologies, Inc. | Amplification of nucleic acid molecules |
| US5270184A (en) | 1991-11-19 | 1993-12-14 | Becton, Dickinson And Company | Nucleic acid target generation |
| ATE231920T1 (de) * | 1992-03-11 | 2003-02-15 | Dana Farber Cancer Inst Inc | Methode um mrna zu klonieren |
| US5262311A (en) * | 1992-03-11 | 1993-11-16 | Dana-Farber Cancer Institute, Inc. | Methods to clone polyA mRNA |
| AU681082B2 (en) * | 1992-05-06 | 1997-08-21 | Gen-Probe Incorporated | Nucleic acid sequence amplification method, composition and kit |
| WO1994003637A1 (en) * | 1992-07-31 | 1994-02-17 | Syntex (Usa) Inc. | Method for introducing defined sequences at the 3' end of polynucleotides |
| US6027923A (en) * | 1993-07-23 | 2000-02-22 | Bio-Rad Laboratories, Inc. | Linked linear amplification of nucleic acids |
| DE69426731T2 (de) * | 1993-11-17 | 2001-06-28 | Amersham Pharmacia Biotech Uk Ltd., Little Chalfont | Verfahren zur massenspektroskopischen sequenzanalyse einer nukleinsäure mittels primerverlängerung |
| CA2140081C (en) | 1994-01-13 | 2008-04-01 | Dean L. Engelhardt | Process, construct and conjugate for producing multiple nucleic acid copies |
| US6335160B1 (en) * | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| FR2724934B1 (fr) * | 1994-09-26 | 1997-01-24 | Bio Merieux | Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique |
| US6280935B1 (en) * | 1994-10-13 | 2001-08-28 | Lynx Therapeutics, Inc. | Method of detecting the presence or absence of a plurality of target sequences using oligonucleotide tags |
| US5665545A (en) * | 1994-11-28 | 1997-09-09 | Akzo Nobel N.V. | Terminal repeat amplification method |
| US5700642A (en) * | 1995-05-22 | 1997-12-23 | Sri International | Oligonucleotide sizing using immobilized cleavable primers |
| US5830655A (en) * | 1995-05-22 | 1998-11-03 | Sri International | Oligonucleotide sizing using cleavable primers |
| FR2737223B1 (fr) * | 1995-07-24 | 1997-09-12 | Bio Merieux | Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres |
| US6068829A (en) * | 1995-09-11 | 2000-05-30 | The Burnham Institute | Method of identifying molecules that home to a selected organ in vivo |
| US5916779A (en) | 1995-09-21 | 1999-06-29 | Becton, Dickinson And Company | Strand displacement amplification of RNA targets |
| US5962271A (en) * | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
| EP2264045B1 (de) * | 1996-08-14 | 2015-10-21 | Life Technologies Corporation | Stabile Zusammensetzungen zur Amplifizierung und Sequenzierung von Nukleinsäuren |
| US5958703A (en) * | 1996-12-03 | 1999-09-28 | Glaxo Group Limited | Use of modified tethers in screening compound libraries |
| US20010000077A1 (en) * | 1998-02-03 | 2001-03-29 | Engelhardt Dean L. | Novel process, construct and conjugate for producing multiple nucleic acid copies |
| US6087103A (en) * | 1998-03-04 | 2000-07-11 | Lifespan Biosciences, Inc. | Tagged ligand arrays for identifying target-ligand interactions |
| GB9817055D0 (en) | 1998-08-05 | 1998-09-30 | Medical Res Council | Reverse transcription and amplification processes and primers therefore |
| ATE439437T1 (de) | 1999-01-05 | 2009-08-15 | Univ Boston | Verbessertes klonierungsverfahren |
| US6358712B1 (en) * | 1999-01-05 | 2002-03-19 | Trustee Of Boston University | Ordered gene assembly |
| US6376246B1 (en) * | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US7074556B2 (en) | 1999-03-02 | 2006-07-11 | Invitrogen Corporation | cDNA synthesis improvements |
| US6132997A (en) * | 1999-05-28 | 2000-10-17 | Agilent Technologies | Method for linear mRNA amplification |
| US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
| US6251639B1 (en) * | 1999-09-13 | 2001-06-26 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences, using a RNA-DNA composite primer |
| US6617442B1 (en) | 1999-09-30 | 2003-09-09 | Isis Pharmaceuticals, Inc. | Human Rnase H1 and oligonucleotide compositions thereof |
| US6794138B1 (en) * | 1999-12-16 | 2004-09-21 | Affymetrix, Inc. | Methods of small sample amplification |
| US7205129B1 (en) | 2000-02-28 | 2007-04-17 | Qiagen Gmbh | Method for reducing artifacts in nucleic acid amplification |
| JP2004513617A (ja) | 2000-06-26 | 2004-05-13 | ニューゲン テクノロジーズ， インコーポレイテッド | 転写に基づく核酸増幅のための方法および組成物 |
| WO2002028876A2 (en) | 2000-10-05 | 2002-04-11 | Riken | Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers. |
| US20020127575A1 (en) | 2000-10-30 | 2002-09-12 | Glenn Hoke | Partially double-stranded nucleic acids, methods of making, and use thereof |
| AU2002228974A1 (en) * | 2000-12-13 | 2002-06-24 | Nugen Technologies, Inc | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
| US6946251B2 (en) * | 2001-03-09 | 2005-09-20 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences using RNA-DNA composite primers |
| US7176025B2 (en) | 2002-03-11 | 2007-02-13 | Nugen Technologies, Inc. | Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination |
| CA2480525A1 (en) | 2002-03-29 | 2003-10-09 | Nugen Technologies, Inc. | Single primer isothermal nucleic acid amplification-enhanced analyte detection and quantification |
-
2003
- 2003-03-11 US US10/386,623 patent/US7176025B2/en not_active Expired - Fee Related
- 2003-03-11 AT AT03717952T patent/ATE405676T1/de not_active IP Right Cessation
- 2003-03-11 AU AU2003222269A patent/AU2003222269A1/en not_active Abandoned
- 2003-03-11 JP JP2003576637A patent/JP2005519621A/ja active Pending
- 2003-03-11 CA CA002478875A patent/CA2478875A1/en not_active Abandoned
- 2003-03-11 DE DE60323067T patent/DE60323067D1/de not_active Expired - Lifetime
- 2003-03-11 WO PCT/US2003/007425 patent/WO2003078645A2/en not_active Ceased
- 2003-03-11 EP EP03717952A patent/EP1488001B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| AU2003222269A1 (en) | 2003-09-29 |
| EP1488001A2 (de) | 2004-12-22 |
| WO2003078645A3 (en) | 2004-03-18 |
| US7176025B2 (en) | 2007-02-13 |
| CA2478875A1 (en) | 2003-09-25 |
| EP1488001A4 (de) | 2006-12-13 |
| JP2005519621A (ja) | 2005-07-07 |
| WO2003078645A2 (en) | 2003-09-25 |
| US20030215926A1 (en) | 2003-11-20 |
| DE60323067D1 (de) | 2008-10-02 |
| EP1488001B1 (de) | 2008-08-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE405676T1 (de) | Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination | |
| DE69433487D1 (de) | Methoden und zusammensetzungen zur effizienten nukleinsaeuresequenzierung | |
| ATE528403T1 (de) | Verfahren zur herstellung von ungesättigten omega-3-fettsäuren in transgenen organismen | |
| ATE205878T1 (de) | Verfahren zur herstellung von polynucleotiden mit erwünschten eigenschaften mittels wiederholter selektion und rekombination | |
| DE60042969D1 (de) | Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren | |
| DE69128545D1 (de) | Technik des genetischen fingerabdrucks mit dns-vervielfältigung | |
| ATE392430T1 (de) | Verfahren zur herstellung von dipeptiden | |
| ATE243753T1 (de) | Mch4 und mch5, apoptotische proteasen, dafür kodierende nukleinsäure und verfahren zur verwendung | |
| DE69928263D1 (de) | Verfahren zur herstellung in vitro von rekombinierten nucleotidsequenzen, und so hergestelte genbanken und sequenzen | |
| IL138002A0 (en) | Methods for making character strings, polynucleotides and polypeptides having desired characteristics | |
| WO1998039485A3 (en) | Compositions and methods for analysis of nucleic acids | |
| ATE262595T1 (de) | Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen | |
| DE69731834D1 (de) | Agouti verwandtes gen | |
| EP1365025A4 (de) | Agarase und gen dafür | |
| WO2003006644A3 (de) | Lipase/acyltransferase | |
| ATE329927T1 (de) | Rekombinante coryneformbakterie die glyceraldehyde-3-phosphate dehydrogenase -2 überexprimieren , und verfahren zur herstellung von l-lysine | |
| ATE340258T1 (de) | Verfahren zur herstellung von nukleinsäuren bestehend aus stochastisch kombinierten anteilen von ausgangsnukleinsäuren | |
| ATE372388T1 (de) | Verfahren zur transformation von tierzellen | |
| DE60110926D1 (de) | Eine methode zur in vitro molekularen evolution der proteinfunktion | |
| ATE506370T1 (de) | Reagenzien und verfahren zur bildung von disulfid-bindungen und glykosylierung von proteinen | |
| WO2001081600A3 (en) | Transgenic plants | |
| ATE519859T1 (de) | Verfahren zur herstellung von ausgewählten intermolekularen quervernetzungen in nukleinsäuren und ihre verwendungen | |
| DE60228867D1 (de) | Verfahren zur herstellung und identifizierung löslicher proteindomänen | |
| ATE329055T1 (de) | Methode zur erstellung von genexpressionsprofilen | |
| DE69632963D1 (de) | Mch3, eine apoptosis-protease, kodierende nukleinsäuren und methoden zur verwendung |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |