ATE405676T1 - Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination - Google Patents

Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination

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Publication number
ATE405676T1
ATE405676T1 AT03717952T AT03717952T ATE405676T1 AT E405676 T1 ATE405676 T1 AT E405676T1 AT 03717952 T AT03717952 T AT 03717952T AT 03717952 T AT03717952 T AT 03717952T AT E405676 T1 ATE405676 T1 AT E405676T1
Authority
AT
Austria
Prior art keywords
double stranded
methods
recombination
complexes
stranded dna
Prior art date
Application number
AT03717952T
Other languages
English (en)
Inventor
Nurith Kurn
Martin Wang
Original Assignee
Nugen Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nugen Technologies Inc filed Critical Nugen Technologies Inc
Application granted granted Critical
Publication of ATE405676T1 publication Critical patent/ATE405676T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT03717952T 2002-03-11 2003-03-11 Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination ATE405676T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US36372902P 2002-03-11 2002-03-11

Publications (1)

Publication Number Publication Date
ATE405676T1 true ATE405676T1 (de) 2008-09-15

Family

ID=28041805

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03717952T ATE405676T1 (de) 2002-03-11 2003-03-11 Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination

Country Status (8)

Country Link
US (1) US7176025B2 (de)
EP (1) EP1488001B1 (de)
JP (1) JP2005519621A (de)
AT (1) ATE405676T1 (de)
AU (1) AU2003222269A1 (de)
CA (1) CA2478875A1 (de)
DE (1) DE60323067D1 (de)
WO (1) WO2003078645A2 (de)

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US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
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JP7016215B2 (ja) 2014-01-27 2022-02-04 ザ ジェネラル ホスピタル コーポレイション 配列決定のために核酸を調製する方法
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
CN107075581B (zh) 2014-08-06 2022-03-18 纽亘技术公司 由靶向测序进行数字测量
CA3037185A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation
JP6997773B2 (ja) 2016-09-15 2022-01-18 アーチャーディーエックス， エルエルシー 無細胞ｄｎａの分析用の核酸サンプル調製の方法
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
CN111742056B (zh) * 2017-06-23 2023-09-01 荣研化学株式会社 核酸检测方法、核酸检测用引物以及核酸检测用试剂盒
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EP3851542A1 (de) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Verarmung von reichlich uninformativen sequenzen
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统

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Also Published As

Publication number Publication date
EP1488001A4 (de) 2006-12-13
AU2003222269A1 (en) 2003-09-29
CA2478875A1 (en) 2003-09-25
WO2003078645A3 (en) 2004-03-18
DE60323067D1 (de) 2008-10-02
JP2005519621A (ja) 2005-07-07
US20030215926A1 (en) 2003-11-20
EP1488001B1 (de) 2008-08-20
WO2003078645A2 (en) 2003-09-25
EP1488001A2 (de) 2004-12-22
US7176025B2 (en) 2007-02-13

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