ATE408709T1 - Homogenes verfahren zum testen von duplex- oder triplex-hybridisierungen mittels durchführung von mehreren messungen unter verschiedenen bedingungen - Google Patents

Homogenes verfahren zum testen von duplex- oder triplex-hybridisierungen mittels durchführung von mehreren messungen unter verschiedenen bedingungen

Info

Publication number
ATE408709T1
ATE408709T1 AT01902567T AT01902567T ATE408709T1 AT E408709 T1 ATE408709 T1 AT E408709T1 AT 01902567 T AT01902567 T AT 01902567T AT 01902567 T AT01902567 T AT 01902567T AT E408709 T1 ATE408709 T1 AT E408709T1
Authority
AT
Austria
Prior art keywords
assay
hybridization
duplex
signals
triplex
Prior art date
Application number
AT01902567T
Other languages
English (en)
Inventor
Glen Erikson
Pierre Picard
Jasmine Daksis
Original Assignee
Ingeneus Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ingeneus Inc filed Critical Ingeneus Inc
Application granted granted Critical
Publication of ATE408709T1 publication Critical patent/ATE408709T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/113—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being electroactive, e.g. redox labels
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
AT01902567T 2000-01-24 2001-01-23 Homogenes verfahren zum testen von duplex- oder triplex-hybridisierungen mittels durchführung von mehreren messungen unter verschiedenen bedingungen ATE408709T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/490,273 US6265170B1 (en) 2000-01-24 2000-01-24 Homogenous assay of duplex of triplex hybridization by means of multiple measurements under varied conditions

Publications (1)

Publication Number Publication Date
ATE408709T1 true ATE408709T1 (de) 2008-10-15

Family

ID=23947351

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01902567T ATE408709T1 (de) 2000-01-24 2001-01-23 Homogenes verfahren zum testen von duplex- oder triplex-hybridisierungen mittels durchführung von mehreren messungen unter verschiedenen bedingungen

Country Status (15)

Country Link
US (1) US6265170B1 (de)
EP (1) EP1250459B1 (de)
JP (1) JP4182149B2 (de)
KR (1) KR100488185B1 (de)
CN (1) CN1253583C (de)
AT (1) ATE408709T1 (de)
AU (1) AU784178B2 (de)
BR (1) BR0107831A (de)
CA (1) CA2398362C (de)
DE (1) DE60135827D1 (de)
HK (1) HK1047455A1 (de)
IL (2) IL150844A0 (de)
MX (1) MXPA02007210A (de)
RU (1) RU2242518C2 (de)
WO (1) WO2001053526A2 (de)

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US6858390B2 (en) * 1998-12-31 2005-02-22 Ingeneus Corporation Aptamers containing sequences of nucleic acid or nucleic acid analogues bound homologously, or in novel complexes
US6656692B2 (en) * 1999-12-21 2003-12-02 Ingeneus Corporation Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US6420115B1 (en) 1999-12-21 2002-07-16 Ingeneus Corporation Cation mediated triplex hybridization assay
US6403313B1 (en) 1999-12-21 2002-06-11 Ingeneus Corporation Fluorescent intensity assay for duplex and triplex nucleic acid hybridization solution utilizing fluorescent intercalators
US6927027B2 (en) * 1999-12-21 2005-08-09 Ingeneus Corporation Nucleic acid multiplex formation
US7052844B2 (en) * 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
US6924108B2 (en) 1999-12-21 2005-08-02 Ingeneus Corporation Nucleic acid binding enhancement by conjugation with nucleotides, nucleosides, bases and/or their analogues
US20030181412A1 (en) * 1999-12-21 2003-09-25 Ingeneus Corporation Method for modifying transcription and/or translation in an organism for therapeutic, prophylactic and/or analytic uses
US7309569B2 (en) 1999-12-21 2007-12-18 Ingeneus, Inc. Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US6613524B1 (en) * 2000-01-24 2003-09-02 Ingeneus Corporation Amperometric affinity assay and electrically stimulated complexes of nucleic acids
US6982147B2 (en) * 2000-01-24 2006-01-03 Ingeneus Corporation Apparatus for assaying biopolymer binding by means of multiple measurements under varied conditions
US7220541B2 (en) * 2000-01-24 2007-05-22 Ingeneus, Inc. Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions
US20030170659A1 (en) * 2000-01-24 2003-09-11 Ingeneus Corporation Electrical treatment of binding media to encourage, discourage and/or study biopolymer binding
DE10155053B4 (de) * 2001-11-09 2005-11-24 Friz Biochem Gesellschaft Für Bioanalytik Mbh Reversible Bindung eines Fluorophors an eine Oberfläche zur Detektion von Ligat-Ligand-Assoziationsereignissen durch Fluoreszenz-Quenchen
DE10155031A1 (de) * 2001-11-09 2003-05-28 Friz Biochem Gmbh Fluoreszenz-Quenchen zur Detektion von Ligat-Ligand Assoziationsereignissen in elektrischen Feldern
JP2005289810A (ja) * 2002-03-18 2005-10-20 Sangaku Renkei Kiko Kyushu:Kk 蛍光色素基およびインターカレート基を含有する二本鎖核酸を選択的に検出するための蛍光試薬
US20040142329A1 (en) * 2003-01-17 2004-07-22 Ingeneus Corporation Probe conjugation to increase multiplex binding motif preference
US20040180345A1 (en) * 2003-03-14 2004-09-16 Ingeneus Corporation Pre-incubation method to improve signal/noise ratio of nucleic acid assays
KR100994752B1 (ko) 2003-03-31 2010-11-16 디지탈 지노믹스(주) 디엔에이 하이브리드형성의 전기적 검출방법과 이를 위한디엔에이칩 및 그 제조방법
GB2422196B (en) 2003-05-20 2008-05-07 Investigen Inc System for detecting polynucleotides
AU2005286084C1 (en) * 2004-09-24 2011-11-24 Ingeneus Inc. Genomic assay
WO2007100711A2 (en) 2006-02-24 2007-09-07 Investigen, Inc. Methods and compositions for detecting polynucleotides
JP5218423B2 (ja) * 2006-12-28 2013-06-26 和光純薬工業株式会社 アッセイシステムの正規化方法
GB0701444D0 (en) * 2007-01-25 2007-03-07 Iti Scotland Ltd Detecting analytes
WO2008103702A2 (en) * 2007-02-23 2008-08-28 Investigen, Inc. Methods and compositions for rapid light-activated isolation and detection of analytes
EP2172563A1 (de) * 2008-09-24 2010-04-07 bioMérieux S.A. Verfahren zur Verringerung der Abhängigkeit von Sequenzvariationen eines Nukleinsäuretargets in einem diagnostischen Hybridisierungs-Assay
MX340258B (es) * 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
GB201120118D0 (en) * 2011-11-22 2012-01-04 Iti Scotland Ltd Detecting analytes

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Also Published As

Publication number Publication date
BR0107831A (pt) 2004-07-27
WO2001053526A3 (en) 2002-06-13
EP1250459B1 (de) 2008-09-17
CN1447861A (zh) 2003-10-08
RU2002122770A (ru) 2004-03-10
IL150844A (en) 2008-04-13
DE60135827D1 (de) 2008-10-30
CN1253583C (zh) 2006-04-26
US6265170B1 (en) 2001-07-24
HK1047455A1 (zh) 2003-02-21
CA2398362A1 (en) 2001-07-26
EP1250459A2 (de) 2002-10-23
CA2398362C (en) 2010-05-04
MXPA02007210A (es) 2002-12-09
KR100488185B1 (en) 2005-05-09
KR20020065940A (ko) 2002-08-14
AU3042501A (en) 2001-07-31
AU784178B2 (en) 2006-02-16
JP4182149B2 (ja) 2008-11-19
IL150844A0 (en) 2003-02-12
WO2001053526A2 (en) 2001-07-26
RU2242518C2 (ru) 2004-12-20
JP2003523738A (ja) 2003-08-12

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