ATE307903T1 - Immunologischer nachweis von rna:dna hybriden auf mikroarrays - Google Patents

Immunologischer nachweis von rna:dna hybriden auf mikroarrays

Info

Publication number
ATE307903T1
ATE307903T1 AT00980379T AT00980379T ATE307903T1 AT E307903 T1 ATE307903 T1 AT E307903T1 AT 00980379 T AT00980379 T AT 00980379T AT 00980379 T AT00980379 T AT 00980379T AT E307903 T1 ATE307903 T1 AT E307903T1
Authority
AT
Austria
Prior art keywords
biological molecules
rna
microarrays
hybrid
kit
Prior art date
Application number
AT00980379T
Other languages
English (en)
Inventor
James G Lazar
Joan M Zakel
Christina M Strange
Inna R Williams
Attila T Lorincz
La Rosa Abel De
Original Assignee
Digene Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/440,419 external-priority patent/US6277579B1/en
Application filed by Digene Corp filed Critical Digene Corp
Application granted granted Critical
Publication of ATE307903T1 publication Critical patent/ATE307903T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT00980379T 1999-11-15 2000-11-14 Immunologischer nachweis von rna:dna hybriden auf mikroarrays ATE307903T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/440,419 US6277579B1 (en) 1998-02-06 1999-11-15 Direct detection of RNA mediated by reverse transcriptase lacking RNAse H function
US09/707,178 US6686151B1 (en) 1998-02-06 2000-11-06 Immunological detection of RNA:DNA hybrids on microarrays
PCT/US2000/031277 WO2001036681A2 (en) 1999-11-15 2000-11-14 Immunological detection of rna:dna hybrids on microarrays

Publications (1)

Publication Number Publication Date
ATE307903T1 true ATE307903T1 (de) 2005-11-15

Family

ID=27032416

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00980379T ATE307903T1 (de) 1999-11-15 2000-11-14 Immunologischer nachweis von rna:dna hybriden auf mikroarrays

Country Status (8)

Country Link
US (1) US6686151B1 (de)
EP (1) EP1230396B1 (de)
AT (1) ATE307903T1 (de)
AU (1) AU784821B2 (de)
BR (1) BRPI0015582B1 (de)
CA (1) CA2391558A1 (de)
DE (1) DE60023539T2 (de)
WO (1) WO2001036681A2 (de)

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US7399589B2 (en) 1998-02-06 2008-07-15 Digene Corporation Immunological detection of RNA:DNA hybrids on microarrays
US7439016B1 (en) * 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
GB0105790D0 (en) * 2001-03-08 2001-04-25 Expresson Biosystems Ltd Detecting binding of mRNA to an oligonnucleotide array using RNA dependent nucleic acid modifying enzymes
US20040161741A1 (en) * 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US9777312B2 (en) 2001-06-30 2017-10-03 Enzo Life Sciences, Inc. Dual polarity analysis of nucleic acids
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
EP1451579A4 (de) * 2001-11-19 2005-12-28 Protometrix Inc Verfahren zur verwendung eines nicht-antikörper-proteins zum nachweis und zur messung eines analyten
EP1507148B1 (de) * 2003-08-25 2005-02-23 MTM Laboratories AG Verfahren zum Nachweis von Karzinomen in solubilisierten zervikalen Körperproben
US20040067492A1 (en) * 2002-10-04 2004-04-08 Allan Peng Reverse transcription on microarrays
US7560232B2 (en) * 2002-12-19 2009-07-14 Promega Corporation Methods of capturing, detecting and quantifying RNA:DNA hybrids and a modified RNase H useful therein
JP3920223B2 (ja) * 2003-01-07 2007-05-30 日本碍子株式会社 反応性チップと、このチップを用いた標的物質の結合検出方法
US20040248323A1 (en) 2003-06-09 2004-12-09 Protometrix, Inc. Methods for conducting assays for enzyme activity on protein microarrays
WO2005003318A2 (en) * 2003-07-02 2005-01-13 Perkinelmer Las, Inc. Assay and process for labeling and detection of micro rna and small interfering rna sequences
US7964344B2 (en) * 2003-09-17 2011-06-21 Canon Kabushiki Kaisha Stable hybrid
EP1689887B1 (de) 2003-10-21 2012-03-21 Orion Genomics, LLC Verfahren zur quantitativen bestimmung der methylierungsdichte in einem dna-locus
WO2006023312A2 (en) * 2004-08-20 2006-03-02 Carl Deirmengian Diagnostic assay for source of inflammation
US20060189893A1 (en) * 2005-01-06 2006-08-24 Diamics, Inc. Systems and methods for detecting abnormal cells
US20060161076A1 (en) * 2005-01-06 2006-07-20 Diamics, Inc. Systems and methods for collection of cell clusters
TWI305803B (en) * 2005-12-05 2009-02-01 Yang Chuen Yeh A method for assaying nucleic acid fragment and kit thereof
US20070172841A1 (en) * 2006-01-25 2007-07-26 Hui Wang Probe/target stabilization with add-in oligo
JP2011518333A (ja) * 2008-04-17 2011-06-23 キアジェン ゲイサーズバーグ インコーポレイテッド 標的核酸の存在を判別するための合成プローブを用いた組成物、方法、およびキット
TWI419974B (zh) 2008-10-27 2013-12-21 Qiagen Gaithersburg Inc 於自動平台上之快速結果雜交捕捉法
WO2010088292A1 (en) * 2009-01-28 2010-08-05 Qiagen Gaithersburg, Inc. Sequence-specific large volume sample preparation method and assay
AU2010242867B2 (en) 2009-05-01 2016-05-12 Qiagen Gaithersburg, Inc. A non-target amplification method for detection of RNA splice-forms in a sample
EP2478087B1 (de) 2009-09-14 2017-01-18 QIAGEN Gaithersburg, Inc. Zusammensetzungen und verfahren zur rückgewinnung von nukleinsäuren oder proteinen aus in zytologischen medien fixierten gewebeproben
US20120021460A1 (en) 2010-01-08 2012-01-26 Qiagen Gaithersburg Inc. Materials and methods for isothermal nucleic acid amplification
WO2011094514A1 (en) * 2010-01-29 2011-08-04 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
JP2013517803A (ja) 2010-01-29 2013-05-20 キアジェン ゲイサーズバーグ インコーポレイテッド サンプル中のｈｐｖの存在を決定および確認する方法
EP2572001A2 (de) 2010-05-19 2013-03-27 QIAGEN Gaithersburg, Inc. Verfahren und zusammensetzungen für sequenzspezifische reinigung und multiplex-analyse von nukleinsäuren
CA2799205A1 (en) 2010-05-25 2011-12-01 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically-truncated probes
CN103517994A (zh) 2011-01-07 2014-01-15 奇亚根盖瑟斯堡股份有限公司 用于对高风险人乳头瘤病毒进行基因型分型和定量的材料和方法
EP2678442B1 (de) 2011-02-24 2019-01-16 QIAGEN Gaithersburg, Inc. Materialien und verfahren für den nachweis von hpv-nukleinsäuren

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Publication number Priority date Publication date Assignee Title
US4732847A (en) 1981-06-09 1988-03-22 University Of Hawaii Monoclonal antibodies for DNA-RNA hybrid complexes and their uses
US4865980A (en) 1982-12-29 1989-09-12 University Of Hawaii Monoclonal antibodies for DNA-RNA hybrid complexes and their uses
US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
ZA853756B (en) 1984-06-01 1986-01-29 Miles Lab Nucleic acid hybridization assay employing immobilized rna probes
IL85551A0 (en) 1987-04-01 1988-08-31 Miles Inc Rapid hybridization assay and reagent system used therein
US5981179A (en) 1991-11-14 1999-11-09 Digene Diagnostics, Inc. Continuous amplification reaction
EP0667918B1 (de) 1991-11-14 2000-02-16 Digene Diagnostics, Inc. Nicht-radioaktives hybridisations testverfahren und satz
CA2139070C (en) 1994-12-23 2010-03-30 Burton W. Blais Method for enhancing detection ability of nucleic acid assays employing polymerase chain reaction
AU735440B2 (en) 1996-02-09 2001-07-05 Cornell Research Foundation Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6043038A (en) 1998-03-31 2000-03-28 Tularik, Inc. High-throughput screening assays for modulators of primase activity
US6225053B1 (en) 1997-12-12 2001-05-01 Digene Corporation Detection of hepatitis B virus
DE69839734D1 (de) 1997-12-22 2008-08-28 Hitachi Chemical Co Ltd Direkte rt-pcr auf mikrotiterplatten mit immobilisierten oligonukleotid
EP1047794A2 (de) 1998-01-13 2000-11-02 BioChip Technologies GmbH Verfahren zum nachweis von nukleinsäuresequenzen
US6280954B1 (en) 1998-02-02 2001-08-28 Amersham Pharmacia Biotech Ab Arrayed primer extension technique for nucleic acid analysis
US5994079A (en) * 1998-02-06 1999-11-30 Digene Corporation Direct detection of RNA mediated by reverse transcriptase lacking RNAse H function
US6232068B1 (en) 1999-01-22 2001-05-15 Rosetta Inpharmatics, Inc. Monitoring of gene expression by detecting hybridization to nucleic acid arrays using anti-heteronucleic acid antibodies
AU4051300A (en) 1999-04-02 2000-10-23 Tropix, Inc. High throughput and high sensitivity detection assays

Also Published As

Publication number Publication date
AU784821B2 (en) 2006-06-29
DE60023539D1 (de) 2005-12-01
DE60023539T2 (de) 2006-07-27
US6686151B1 (en) 2004-02-03
WO2001036681A2 (en) 2001-05-25
WO2001036681A3 (en) 2001-12-13
BR0015582A (pt) 2003-06-10
EP1230396B1 (de) 2005-10-26
BRPI0015582B1 (pt) 2017-02-14
EP1230396A2 (de) 2002-08-14
CA2391558A1 (en) 2001-05-25
AU1764601A (en) 2001-05-30

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