ATE412760T1 - Verfahren zur einführung von inhibitorischer rna in pflanzen und deren anwendungen - Google Patents

Verfahren zur einführung von inhibitorischer rna in pflanzen und deren anwendungen

Info

Publication number
ATE412760T1
ATE412760T1 AT00922647T AT00922647T ATE412760T1 AT E412760 T1 ATE412760 T1 AT E412760T1 AT 00922647 T AT00922647 T AT 00922647T AT 00922647 T AT00922647 T AT 00922647T AT E412760 T1 ATE412760 T1 AT E412760T1
Authority
AT
Austria
Prior art keywords
plants
inhibitory rna
applications
identification
introducing inhibitory
Prior art date
Application number
AT00922647T
Other languages
English (en)
Inventor
Frank Meulewaeter
Marc Cornelissen
John Jacobs
Eldik Gerben Van
Michael Metzlaff
Original Assignee
Bayer Bioscience Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Bioscience Nv filed Critical Bayer Bioscience Nv
Application granted granted Critical
Publication of ATE412760T1 publication Critical patent/ATE412760T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT00922647T 1999-04-20 2000-04-17 Verfahren zur einführung von inhibitorischer rna in pflanzen und deren anwendungen ATE412760T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US29402299A 1999-04-20 1999-04-20

Publications (1)

Publication Number Publication Date
ATE412760T1 true ATE412760T1 (de) 2008-11-15

Family

ID=23131564

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00922647T ATE412760T1 (de) 1999-04-20 2000-04-17 Verfahren zur einführung von inhibitorischer rna in pflanzen und deren anwendungen

Country Status (8)

Country Link
EP (1) EP1173594B1 (de)
AT (1) ATE412760T1 (de)
AU (1) AU775188B2 (de)
CA (1) CA2370515C (de)
DE (1) DE60040656D1 (de)
DK (1) DK1173594T3 (de)
ES (1) ES2316363T3 (de)
WO (1) WO2000063397A2 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7351578B2 (en) 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
CN101818145A (zh) 1998-03-20 2010-09-01 联邦科学和工业研究组织 控制基因表达
US6423885B1 (en) 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
AUPR621501A0 (en) 2001-07-06 2001-08-02 Commonwealth Scientific And Industrial Research Organisation Delivery of ds rna
EP1446503A2 (de) 2001-09-27 2004-08-18 University Of Delaware Zusammensetzung und verfahren zur verbesserung der oligonukleotidgesteuerten veränderung von nukleinsäuresequenzen
WO2003052108A2 (en) * 2001-12-18 2003-06-26 Bayer Bioscience N.V. Improved methods and means for delivering inhibitory rna to plants and applications thereof
JP2005537015A (ja) * 2002-09-04 2005-12-08 ジョンソン アンド ジョンソン リサーチ プロプライアトリー リミテッド ＲＮＡ干渉（ＲＮＡｉ）を媒介するためにｄｓＤＮＡを使用する方法
CN1750752A (zh) * 2003-02-19 2006-03-22 联邦科学和工业研究组织 使用短dsRNA序列在植物中进行有效的基因沉默
US7786349B2 (en) 2003-04-09 2010-08-31 Bayer Bioscience N.V. Methods and means for increasing the tolerance of plants to stress conditions
WO2005054438A2 (en) 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
WO2005098004A2 (en) * 2004-04-07 2005-10-20 Bayer Bioscience N.V. Inducible boost of integrated satellite rna viruses
WO2007131699A2 (en) 2006-05-12 2007-11-22 Bayer Bioscience N.V. Novel stress-related microrna molecules and uses thereof
US12428641B2 (en) 2017-09-15 2025-09-30 Commonwealth Scientific And Industrial Research Organisation RNA molecules
CN114369617B (zh) * 2022-03-16 2022-06-28 广东省农业科学院植物保护研究所 一种油菜花叶病毒侵染性克隆载体及其构建方法和应用
CN119876176A (zh) * 2025-01-10 2025-04-25 四川省农业科学院园艺研究所 一种基于rna病毒诱导的叶菜类蔬菜沉默体系及其构建方法和应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5922602A (en) * 1988-02-26 1999-07-13 Biosource Technologies, Inc. Cytoplasmic inhibition of gene expression
JPH0253491A (ja) * 1988-08-17 1990-02-22 Japan Tobacco Inc ベクター及びその利用方法
WO1990012107A1 (en) * 1989-03-31 1990-10-18 The Salk Institute Biotechnology/Industrial Associates, Inc. Recombinant expression system based on satellite tobacco mosaic virus
GB9703146D0 (en) * 1997-02-14 1997-04-02 Innes John Centre Innov Ltd Methods and means for gene silencing in transgenic plants
GB9710475D0 (en) * 1997-05-21 1997-07-16 Zeneca Ltd Gene silencing
EP1045899A2 (de) * 1998-01-16 2000-10-25 Biosource Technologies, Inc. Methode zur bestimmung der funktion von nukleinsäurensequenzen und davon kodierten proteine durch transfektion in einem gast

Also Published As

Publication number Publication date
CA2370515C (en) 2011-08-09
AU775188B2 (en) 2004-07-22
AU4296500A (en) 2000-11-02
WO2000063397A2 (en) 2000-10-26
CA2370515A1 (en) 2000-10-26
EP1173594B1 (de) 2008-10-29
ES2316363T3 (es) 2009-04-16
EP1173594A2 (de) 2002-01-23
DK1173594T3 (da) 2009-02-23
WO2000063397A3 (en) 2001-02-01
DE60040656D1 (de) 2008-12-11

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