ATE414147T1 - Nukleinsäurereinigung - Google Patents
NukleinsäurereinigungInfo
- Publication number
- ATE414147T1 ATE414147T1 AT02252249T AT02252249T ATE414147T1 AT E414147 T1 ATE414147 T1 AT E414147T1 AT 02252249 T AT02252249 T AT 02252249T AT 02252249 T AT02252249 T AT 02252249T AT E414147 T1 ATE414147 T1 AT E414147T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- plasmid dna
- concentration
- retentate
- acid solution
- Prior art date
Links
- 238000001821 nucleic acid purification Methods 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 239000013612 plasmid Substances 0.000 abstract 3
- 150000001768 cations Chemical class 0.000 abstract 2
- 239000000356 contaminant Substances 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 239000012465 retentate Substances 0.000 abstract 2
- 230000007717 exclusion Effects 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 238000000108 ultra-filtration Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Saccharide Compounds (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0107634.8A GB0107634D0 (en) | 2001-03-27 | 2001-03-27 | Nucleic acid purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE414147T1 true ATE414147T1 (de) | 2008-11-15 |
Family
ID=9911655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02252249T ATE414147T1 (de) | 2001-03-27 | 2002-03-27 | Nukleinsäurereinigung |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US7811807B2 (de) |
| EP (1) | EP1245674B9 (de) |
| AT (1) | ATE414147T1 (de) |
| DE (1) | DE60229805D1 (de) |
| DK (1) | DK1245674T3 (de) |
| EE (1) | EE05203B1 (de) |
| ES (1) | ES2315340T3 (de) |
| GB (1) | GB0107634D0 (de) |
| PT (1) | PT1245674E (de) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4723254B2 (ja) * | 2002-12-23 | 2011-07-13 | バイカル インコーポレイテッド | プラスミドｄｎａを精製するための方法 |
| US9163229B2 (en) | 2006-10-10 | 2015-10-20 | Trovagene, Inc. | Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media |
| GB0913160D0 (en) * | 2009-07-29 | 2009-09-02 | Avecia Biolog Ltd | Extraction process |
| JP2013523143A (ja) | 2010-04-08 | 2013-06-17 | キアゲン ゲーエムベーハー | 核酸を単離および精製するための方法 |
| US20130023655A1 (en) * | 2010-04-08 | 2013-01-24 | Qiagen Gmbh | Method for precipitating anionic surfactant ions in the presence of nucleic acids |
| EP2556158B1 (de) | 2010-04-08 | 2019-12-18 | Qiagen GmbH | Chromatografievorrichtung und Verfahren zum Isolieren und Reinigen von Nukleinsäuren |
| EP2395082A1 (de) | 2010-06-14 | 2011-12-14 | QIAGEN GmbH | Extraktion von Nukleinsäuren aus in Wachs eingebetteten Proben |
| WO2016180430A1 (en) | 2015-05-08 | 2016-11-17 | Curevac Ag | Method for producing rna |
| EP4660315A3 (de) | 2015-05-29 | 2026-03-18 | CureVac Manufacturing GmbH | Verfahren zur herstellung und reinigung von rna mit mindestens einem schritt der tangentialen strömungsfiltration |
| CN106884011B (zh) * | 2015-12-16 | 2020-11-17 | 固安鼎泰海规生物科技有限公司 | 一种规模化质粒纯化的联合液相层析分离方法 |
| CN113186185B (zh) * | 2020-01-14 | 2023-05-26 | 东北林业大学 | 一种从哺乳动物粪便中高效富集宿主dna的方法 |
| CN114907430B (zh) * | 2022-06-21 | 2023-12-01 | 中国科学院过程工程研究所 | 一种mRNA的分离纯化方法 |
| CN115125240B (zh) * | 2022-07-29 | 2025-08-01 | 苏州纳微科技股份有限公司 | 一种质粒dna工业化分离纯化方法及其应用 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3308932A1 (de) * | 1983-03-12 | 1984-09-13 | Hoechst Ag, 6230 Frankfurt | Verfahren zur abtrennung von ribonukleinsaeuren aus einer loesung, die desoxyribonukleinsaeuren enthaelt |
| DE176971T1 (de) * | 1984-09-29 | 1986-11-27 | Wakamoto Pharmaceutical Co., Ltd., Tokio/Tokyo | Fuer ein thermostabiles beta-galaktosidase, kodierendes gen, dieses gen enthaltender bacillus-subtilis, das durch dieses gen kodiertes enzym und verfahren zu dessen herstellung. |
| IT1240870B (it) | 1990-02-14 | 1993-12-17 | Talent | Procedimento per l'estrazione e la purificazione di dna genomico umano |
| US5256294A (en) * | 1990-09-17 | 1993-10-26 | Genentech, Inc. | Tangential flow filtration process and apparatus |
| JPH04360686A (ja) | 1991-06-04 | 1992-12-14 | Tosoh Corp | Ｄｎａの精製方法 |
| AR003122A1 (es) | 1995-05-19 | 1998-07-08 | Merck & Co Inc | Un proceso para aislacion y purificacion de plasmidos en fermentadores de gran escala y el adn aislado y purificado obtenido mediante dicho proceso. |
| US6011148A (en) * | 1996-08-01 | 2000-01-04 | Megabios Corporation | Methods for purifying nucleic acids |
| US7807822B2 (en) * | 1996-08-01 | 2010-10-05 | Robert Bridenbaugh | Methods for purifying nucleic acids |
| WO1998054195A1 (en) | 1997-05-30 | 1998-12-03 | Bion, Inc. | Large-scale isolation and purification of hif |
| SE9703532D0 (sv) * | 1997-09-30 | 1997-09-30 | Pharmacia Biotech Ab | A process for the purification of plasmid DNA |
| US6214586B1 (en) | 1997-12-08 | 2001-04-10 | Genzyme Corporation | Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA |
| US6441160B2 (en) | 1998-05-11 | 2002-08-27 | Tosoh Corporation | Separating plasmids from contaminants using hydrophobic or hydrophobic and ion exchange chromatography |
| RU2001135838A (ru) * | 1999-05-28 | 2004-01-20 | Камбрекс Байо Сайенс Инк. (Us) | Способы очистки ДНК и очищенная ДНК |
| CA2378944A1 (en) * | 1999-07-23 | 2001-02-01 | Genentech, Inc. | Method for rnase- and organic solvent-free plasmid dna purification using tangential flow filtration |
| US6350382B1 (en) * | 2000-06-23 | 2002-02-26 | Scilog, Inc. | Enhancing filtration yields in tangential flow filtration |
| GB0028361D0 (en) * | 2000-11-21 | 2001-01-03 | Glaxo Group Ltd | Method of separating extra chromosomal dna from other cellular components |
-
2001
- 2001-03-27 GB GBGB0107634.8A patent/GB0107634D0/en not_active Ceased
-
2002
- 2002-03-27 ES ES02252249T patent/ES2315340T3/es not_active Expired - Lifetime
- 2002-03-27 AT AT02252249T patent/ATE414147T1/de not_active IP Right Cessation
- 2002-03-27 EP EP02252249A patent/EP1245674B9/de not_active Expired - Lifetime
- 2002-03-27 DE DE60229805T patent/DE60229805D1/de not_active Expired - Lifetime
- 2002-03-27 DK DK02252249T patent/DK1245674T3/da active
- 2002-03-27 EE EEP200200164A patent/EE05203B1/xx not_active IP Right Cessation
- 2002-03-27 PT PT02252249T patent/PT1245674E/pt unknown
- 2002-03-27 US US10/106,824 patent/US7811807B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| DK1245674T3 (da) | 2009-01-19 |
| GB0107634D0 (en) | 2001-05-16 |
| EP1245674A2 (de) | 2002-10-02 |
| ES2315340T3 (es) | 2009-04-01 |
| EP1245674B9 (de) | 2009-10-21 |
| EP1245674B1 (de) | 2008-11-12 |
| PT1245674E (pt) | 2008-12-23 |
| US7811807B2 (en) | 2010-10-12 |
| US20030109696A1 (en) | 2003-06-12 |
| EE200200164A (et) | 2002-12-16 |
| EE05203B1 (et) | 2009-08-17 |
| DE60229805D1 (de) | 2008-12-24 |
| EP1245674A3 (de) | 2003-12-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 1245674 Country of ref document: EP |
|
| REN | Ceased due to non-payment of the annual fee |