EE05203B1 - Nukleiinhapete puhastamine - Google Patents

Nukleiinhapete puhastamine

Info

Publication number
EE05203B1
EE05203B1 EEP200200164A EEP200200164A EE05203B1 EE 05203 B1 EE05203 B1 EE 05203B1 EE P200200164 A EEP200200164 A EE P200200164A EE P200200164 A EEP200200164 A EE P200200164A EE 05203 B1 EE05203 B1 EE 05203B1
Authority
EE
Estonia
Prior art keywords
nucleic acid
plasmid dna
concentration
retentate
acid solution
Prior art date
Application number
EEP200200164A
Other languages
English (en)
Estonian (et)
Inventor
Kvederas Rimantas
Siksniute Asta
Markauskas Algimantas
Original Assignee
Fermentas Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fermentas Ab filed Critical Fermentas Ab
Publication of EE200200164A publication Critical patent/EE200200164A/xx
Publication of EE05203B1 publication Critical patent/EE05203B1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
EEP200200164A 2001-03-27 2002-03-27 Nukleiinhapete puhastamine EE05203B1 (et)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB0107634.8A GB0107634D0 (en) 2001-03-27 2001-03-27 Nucleic acid purification

Publications (2)

Publication Number Publication Date
EE200200164A EE200200164A (et) 2002-12-16
EE05203B1 true EE05203B1 (et) 2009-08-17

Family

ID=9911655

Family Applications (1)

Application Number Title Priority Date Filing Date
EEP200200164A EE05203B1 (et) 2001-03-27 2002-03-27 Nukleiinhapete puhastamine

Country Status (9)

Country Link
US (1) US7811807B2 (de)
EP (1) EP1245674B9 (de)
AT (1) ATE414147T1 (de)
DE (1) DE60229805D1 (de)
DK (1) DK1245674T3 (de)
EE (1) EE05203B1 (de)
ES (1) ES2315340T3 (de)
GB (1) GB0107634D0 (de)
PT (1) PT1245674E (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004060277A2 (en) * 2002-12-23 2004-07-22 Vical Incorporated Process for purification of plasmid dna
ES2588227T3 (es) 2006-10-10 2016-10-31 Trovagene, Inc. Composiciones, métodos y kits para aislar ácidos nucleicos de fluidos corporales usando medios de intercambio aniónico
GB0913160D0 (en) * 2009-07-29 2009-09-02 Avecia Biolog Ltd Extraction process
EP2556158B1 (de) 2010-04-08 2019-12-18 Qiagen GmbH Chromatografievorrichtung und Verfahren zum Isolieren und Reinigen von Nukleinsäuren
CN102822339A (zh) * 2010-04-08 2012-12-12 恰根有限公司 在核酸存在下沉淀阴离子表面活性剂离子的方法
WO2011124705A1 (en) * 2010-04-08 2011-10-13 Qiagen Gmbh Method for isolating and purifying nucleic acids
EP2395082A1 (de) 2010-06-14 2011-12-14 QIAGEN GmbH Extraktion von Nukleinsäuren aus in Wachs eingebetteten Proben
WO2016180430A1 (en) 2015-05-08 2016-11-17 Curevac Ag Method for producing rna
DK3303583T3 (da) 2015-05-29 2020-07-13 Curevac Real Estate Gmbh Fremgangsmåde til fremstilling og oprensning af rna, omfattende mindst et trin til tangential-flow-filtrering
CN106884011B (zh) * 2015-12-16 2020-11-17 固安鼎泰海规生物科技有限公司 一种规模化质粒纯化的联合液相层析分离方法
CN113186185B (zh) * 2020-01-14 2023-05-26 东北林业大学 一种从哺乳动物粪便中高效富集宿主dna的方法
CN114907430B (zh) * 2022-06-21 2023-12-01 中国科学院过程工程研究所 一种mRNA的分离纯化方法
CN115125240B (zh) * 2022-07-29 2025-08-01 苏州纳微科技股份有限公司 一种质粒dna工业化分离纯化方法及其应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3308932A1 (de) 1983-03-12 1984-09-13 Hoechst Ag, 6230 Frankfurt Verfahren zur abtrennung von ribonukleinsaeuren aus einer loesung, die desoxyribonukleinsaeuren enthaelt
US4861718A (en) * 1984-09-29 1989-08-29 Wakamoto Pharmaceutical Co., Ltd. Gene coding for thermostable beta-galactosidase, bacillus subtilis having the gene, enzyme coded by the gene and a process for the production thereof
IT1240870B (it) * 1990-02-14 1993-12-17 Talent Procedimento per l'estrazione e la purificazione di dna genomico umano
US5256294A (en) * 1990-09-17 1993-10-26 Genentech, Inc. Tangential flow filtration process and apparatus
JPH04360686A (ja) * 1991-06-04 1992-12-14 Tosoh Corp Ｄｎａの精製方法
AR003122A1 (es) * 1995-05-19 1998-07-08 Merck & Co Inc Un proceso para aislacion y purificacion de plasmidos en fermentadores de gran escala y el adn aislado y purificado obtenido mediante dicho proceso.
US7807822B2 (en) 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US6011148A (en) * 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
AU7701398A (en) 1997-05-30 1998-12-30 Bion, Inc. Large-scale isolation and purification of hif
SE9703532D0 (sv) * 1997-09-30 1997-09-30 Pharmacia Biotech Ab A process for the purification of plasmid DNA
US6214586B1 (en) 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
DE69922740T2 (de) 1998-05-11 2005-12-08 Tosoh Corp., Shinnanyo Methode zur Trennung von Nucleinsäuren mittels Flüssigchromatographie
CN1264854C (zh) * 1999-05-28 2006-07-19 坎布雷科斯生物科学巴尔的摩公司 Dna纯化方法和纯化的dna
JP2003505077A (ja) * 1999-07-23 2003-02-12 ジェネンテック・インコーポレーテッド 平行流ろ過法を用いるｒｎａｓｅ−及び有機溶媒−非含有プラスミドｄｎａの精製方法
US6350382B1 (en) * 2000-06-23 2002-02-26 Scilog, Inc. Enhancing filtration yields in tangential flow filtration
GB0028361D0 (en) * 2000-11-21 2001-01-03 Glaxo Group Ltd Method of separating extra chromosomal dna from other cellular components

Also Published As

Publication number Publication date
DK1245674T3 (da) 2009-01-19
DE60229805D1 (de) 2008-12-24
EP1245674A3 (de) 2003-12-17
EP1245674B9 (de) 2009-10-21
US20030109696A1 (en) 2003-06-12
ES2315340T3 (es) 2009-04-01
US7811807B2 (en) 2010-10-12
PT1245674E (pt) 2008-12-23
EP1245674B1 (de) 2008-11-12
EP1245674A2 (de) 2002-10-02
EE200200164A (et) 2002-12-16
GB0107634D0 (en) 2001-05-16
ATE414147T1 (de) 2008-11-15

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Legal Events

Date Code Title Description
HC1A Change of owner name
KB4A Valid patent at the end of a year

Effective date: 20101231

MM4A Lapsed by not paying the annual fees

Effective date: 20120327