ATE418725T1 - Verfahren zur verifikation der verwendung eines bestimmten färbemittels für eine zellprobe - Google Patents

Verfahren zur verifikation der verwendung eines bestimmten färbemittels für eine zellprobe

Info

Publication number
ATE418725T1
ATE418725T1 AT00973976T AT00973976T ATE418725T1 AT E418725 T1 ATE418725 T1 AT E418725T1 AT 00973976 T AT00973976 T AT 00973976T AT 00973976 T AT00973976 T AT 00973976T AT E418725 T1 ATE418725 T1 AT E418725T1
Authority
AT
Austria
Prior art keywords
verifying
cell sample
staining agent
methods
particular staining
Prior art date
Application number
AT00973976T
Other languages
English (en)
Inventor
David Zahniser
Norman Soule
Louise Isenstein
Original Assignee
Cytyc Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/430,198 external-priority patent/US7006674B1/en
Priority claimed from US09/430,117 external-priority patent/US6665060B1/en
Priority claimed from US09/430,196 external-priority patent/US6661501B1/en
Priority claimed from US09/430,116 external-priority patent/US6348325B1/en
Application filed by Cytyc Corp filed Critical Cytyc Corp
Application granted granted Critical
Publication of ATE418725T1 publication Critical patent/ATE418725T1/de

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429Signal processing
    • G01N15/1433Signal processing using image recognition
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484Optical investigation techniques, e.g. flow cytometry microstructural devices
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/314Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
    • G01N21/3151Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths using two sources of radiation of different wavelengths
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/34Microscope slides, e.g. mounting specimens on microscope slides
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • G06T7/0002Inspection of images, e.g. flaw detection
    • G06T7/0012Biomedical image inspection
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • G06T7/70Determining position or orientation of objects or cameras
    • G06T7/73Determining position or orientation of objects or cameras using feature-based methods
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06VIMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V10/00Arrangements for image or video recognition or understanding
    • G06V10/20Image preprocessing
    • G06V10/24Aligning, centring, orientation detection or correction of the image
    • G06V10/245Aligning, centring, orientation detection or correction of the image by locating a pattern; Special marks for positioning
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06VIMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00Scenes; Scene-specific elements
    • G06V20/60Type of objects
    • G06V20/69Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/693Acquisition
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1006Investigating individual particles for cytology
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N2015/1472Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle with colour
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1488Methods for deciding
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/20Special algorithmic details
    • G06T2207/20092Interactive image processing based on input by user
    • G06T2207/20104Interactive definition of region of interest [ROI]
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/30Subject of image; Context of image processing
    • G06T2207/30004Biomedical image processing
    • G06T2207/30016Brain
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/30Subject of image; Context of image processing
    • G06T2207/30004Biomedical image processing
    • G06T2207/30024Cell structures in vitro; Tissue sections in vitro

Landscapes

  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Theoretical Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Multimedia (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Dispersion Chemistry (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Signal Processing (AREA)
  • Optics & Photonics (AREA)
  • Medical Informatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Quality & Reliability (AREA)
  • Toxicology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Lubricants (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Spectrometry And Color Measurement (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT00973976T 1999-10-29 2000-10-27 Verfahren zur verifikation der verwendung eines bestimmten färbemittels für eine zellprobe ATE418725T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/430,198 US7006674B1 (en) 1999-10-29 1999-10-29 Apparatus and methods for verifying the location of areas of interest within a sample in an imaging system
US09/430,117 US6665060B1 (en) 1999-10-29 1999-10-29 Cytological imaging system and method
US09/430,196 US6661501B1 (en) 1999-10-29 1999-10-29 Cytological stain composition including verification characteristic
US09/430,116 US6348325B1 (en) 1999-10-29 1999-10-29 Cytological stain composition

Publications (1)

Publication Number Publication Date
ATE418725T1 true ATE418725T1 (de) 2009-01-15

Family

ID=27503781

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00973976T ATE418725T1 (de) 1999-10-29 2000-10-27 Verfahren zur verifikation der verwendung eines bestimmten färbemittels für eine zellprobe

Country Status (7)

Country Link
EP (1) EP1238260B1 (de)
JP (1) JP4664558B2 (de)
AT (1) ATE418725T1 (de)
AU (1) AU777874B2 (de)
DE (1) DE60041203D1 (de)
HK (1) HK1049516B (de)
WO (1) WO2001033197A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006053117A (ja) * 2004-07-12 2006-02-23 Pola Chem Ind Inc 角層細胞の染色液及びそれを用いた角層細胞の染色方法
ES2326061B1 (es) * 2007-11-20 2010-05-31 Univ. De Valladolid Mezcla de colorantes fluorescentes y procedimientos para teñir el nucleo y el citoplasma celular que permite estudiar la morfologia de las celulas en cultivo sobre cualquier tipo de soporte.
EP3988985B1 (de) * 2011-02-01 2024-08-28 Roche Diagnostics Hematology, Inc. Schnelle autofokussierung in der mikroskopischen bildgebung
US9041791B2 (en) 2011-02-01 2015-05-26 Roche Diagnostics Hematology, Inc. Fast auto-focus in imaging
JP5789786B2 (ja) 2012-11-27 2015-10-07 パナソニックIpマネジメント株式会社 画像計測装置および画像計測方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5008185A (en) * 1985-11-04 1991-04-16 Cell Analysis Systems, Inc. Methods and apparatus for the quantitation of nuclear proteins
DE69031682T2 (de) * 1990-03-07 1998-03-05 Cytyc Corp Thionin Färbungstechnik
US6445451B1 (en) * 1998-02-12 2002-09-03 Hamilton Thorne Research Colorimeter and assay device

Also Published As

Publication number Publication date
EP1238260A1 (de) 2002-09-11
EP1238260B1 (de) 2008-12-24
AU777874B2 (en) 2004-11-04
JP2003513270A (ja) 2003-04-08
JP4664558B2 (ja) 2011-04-06
HK1049516B (en) 2009-03-06
DE60041203D1 (de) 2009-02-05
AU1241601A (en) 2001-05-14
WO2001033197A1 (en) 2001-05-10
HK1049516A1 (en) 2003-05-16

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