ATE423199T1 - Verfahren zur herstelllung von menschlichem thrombin mittels genmodifikationstechnik - Google Patents
Verfahren zur herstelllung von menschlichem thrombin mittels genmodifikationstechnikInfo
- Publication number
- ATE423199T1 ATE423199T1 AT02743820T AT02743820T ATE423199T1 AT E423199 T1 ATE423199 T1 AT E423199T1 AT 02743820 T AT02743820 T AT 02743820T AT 02743820 T AT02743820 T AT 02743820T AT E423199 T1 ATE423199 T1 AT E423199T1
- Authority
- AT
- Austria
- Prior art keywords
- human thrombin
- prethrombin
- human
- producing human
- thrombin
- Prior art date
Links
- 229960003766 thrombin (human) Drugs 0.000 title abstract 7
- 238000012239 gene modification Methods 0.000 title abstract 3
- 230000005017 genetic modification Effects 0.000 title 1
- 235000013617 genetically modified food Nutrition 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
- 108010071286 prethrombins Proteins 0.000 abstract 4
- 230000004913 activation Effects 0.000 abstract 2
- 238000006243 chemical reaction Methods 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 2
- 108010085662 ecarin Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 239000011541 reaction mixture Substances 0.000 abstract 2
- 210000004102 animal cell Anatomy 0.000 abstract 1
- 239000006143 cell culture medium Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 230000002439 hemostatic effect Effects 0.000 abstract 1
- 230000010534 mechanism of action Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21005—Thrombin (3.4.21.5)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Hematology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Diabetes (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001206919 | 2001-07-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE423199T1 true ATE423199T1 (de) | 2009-03-15 |
Family
ID=19042979
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02743820T ATE423199T1 (de) | 2001-07-06 | 2002-07-04 | Verfahren zur herstelllung von menschlichem thrombin mittels genmodifikationstechnik |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US7635577B2 (de) |
| EP (1) | EP1405910B1 (de) |
| JP (1) | JP4227012B2 (de) |
| KR (2) | KR100983878B1 (de) |
| CN (1) | CN100347297C (de) |
| AT (1) | ATE423199T1 (de) |
| CA (1) | CA2452768C (de) |
| DE (1) | DE60231210D1 (de) |
| WO (1) | WO2003004641A1 (de) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040219140A1 (en) * | 2001-02-22 | 2004-11-04 | Reiner Class | Compositions and methods for preventing platelet aggregation |
| WO2005010178A1 (ja) | 2003-07-29 | 2005-02-03 | Juridical Foundation The Chemo-Sero-Therapeutic Research Institute | 組換えフィブリノゲン高産生細胞の作製方法及び高産生細胞 |
| GB0324044D0 (en) * | 2003-10-14 | 2003-11-19 | Astrazeneca Ab | Protein |
| EP1676911B1 (de) | 2003-10-24 | 2010-04-21 | Juridical Foundation, The Chemo-Sero-Therapeutic Research Institute | Neue rekombinante tierzellen mit hoher proteinproduktion, verfahren zur konstruktion davon sowie verfahren zur massenproduktion von protein unter verwendung davon |
| MXPA06013698A (es) * | 2004-05-24 | 2007-08-15 | Panacos Pharmaceuticals Inc | Inhibicion de replicacion de virus 1 de inmunodeficiencia humana (vih-1) por interrupcion del procesamiento de la proteina de capsida-peptido separador 1 viral. |
| EP1874928A1 (de) * | 2005-04-13 | 2008-01-09 | AstraZeneca AB | Wirtszelle mit vektor zur herstellung von gamma-carboxylierung benötigenden proteinen |
| EP1939283A4 (de) | 2005-09-30 | 2009-02-25 | Chemo Sero Therapeut Res Inst | Verfahren zur produktion von rekombinantem menschlichem thrombin durch eine kultivierte zelle |
| US20100159512A1 (en) * | 2006-03-06 | 2010-06-24 | Kurt Osther | Method for the preparation of recombinant human thrombin and fibrinogen |
| US8206967B2 (en) * | 2007-07-06 | 2012-06-26 | Medimmune Limited | Method for production of recombinant human thrombin |
| AU2009236960A1 (en) | 2008-04-16 | 2009-10-22 | The Chemo-Sero-Therapeutic Research Institute | Method of producing thrombin-immobilized bioabsorbable sheet preparation |
| JPWO2014103608A1 (ja) | 2012-12-25 | 2017-01-12 | 一般財団法人化学及血清療法研究所 | ＨＰＶ／ＨＢｓキメラタンパク質を有効成分とするＨＰＶ感染症及び／又はＢ型肝炎用ワクチン |
| JP6216647B2 (ja) | 2013-03-15 | 2017-10-18 | シスメックス株式会社 | 組換え型プロトロンビンの製造方法およびその利用 |
| WO2025208342A1 (zh) * | 2024-04-02 | 2025-10-09 | 苏州泽璟生物制药股份有限公司 | 高纯度且低残留的重组人凝血酶制备方法及其应用 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2824434B2 (ja) * | 1989-11-28 | 1998-11-11 | 財団法人化学及血清療法研究所 | 新規発現ベクター |
| US5278144A (en) | 1990-09-04 | 1994-01-11 | Cor Therapeutics, Inc. | Antithrombosis agents |
| US5583107A (en) | 1990-09-04 | 1996-12-10 | Cor Therapeutics, Inc. | Agents affecting thrombosis and hemostasis |
| CA2078721A1 (en) * | 1991-09-24 | 1993-03-25 | Hiroshi Yonemura | Process for preparing human coagulation factor viii protein complex |
| JP3168087B2 (ja) * | 1993-01-11 | 2001-05-21 | 株式会社ヤスヰ | 洗車方法及びその装置 |
| AT404357B (de) | 1995-06-13 | 1998-11-25 | Immuno Ag | Prothrombin-derivate |
| JP2002514433A (ja) | 1998-05-14 | 2002-05-21 | バッテル メモリアル インスティテュート | トランスジェニック植物由来ヒト凝血因子 |
| JP2003504082A (ja) | 1999-07-09 | 2003-02-04 | コヘージョン テクノロジーズ， インコーポレイテッド | エカリンポリペプチド、エカリンをコードするポリヌクレオチド、及びその利用のための方法 |
| CN1298017A (zh) * | 1999-11-29 | 2001-06-06 | 中国科学技术大学 | 一种快速活化凝血酶原的方法 |
-
2002
- 2002-07-04 DE DE60231210T patent/DE60231210D1/de not_active Expired - Lifetime
- 2002-07-04 JP JP2003510799A patent/JP4227012B2/ja not_active Expired - Fee Related
- 2002-07-04 AT AT02743820T patent/ATE423199T1/de active
- 2002-07-04 EP EP02743820A patent/EP1405910B1/de not_active Expired - Lifetime
- 2002-07-04 CN CNB028175034A patent/CN100347297C/zh not_active Expired - Fee Related
- 2002-07-04 CA CA2452768A patent/CA2452768C/en not_active Expired - Fee Related
- 2002-07-04 WO PCT/JP2002/006771 patent/WO2003004641A1/ja not_active Ceased
- 2002-07-04 KR KR1020097015790A patent/KR100983878B1/ko not_active Expired - Fee Related
- 2002-07-04 US US10/482,926 patent/US7635577B2/en not_active Expired - Fee Related
- 2002-07-04 KR KR10-2004-7000118A patent/KR20040032147A/ko not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| CN1551917A (zh) | 2004-12-01 |
| KR20090087136A (ko) | 2009-08-14 |
| EP1405910B1 (de) | 2009-02-18 |
| WO2003004641A1 (en) | 2003-01-16 |
| US7635577B2 (en) | 2009-12-22 |
| CA2452768A1 (en) | 2003-01-16 |
| DE60231210D1 (de) | 2009-04-02 |
| EP1405910A4 (de) | 2005-02-02 |
| KR20040032147A (ko) | 2004-04-14 |
| EP1405910A1 (de) | 2004-04-07 |
| JP4227012B2 (ja) | 2009-02-18 |
| KR100983878B1 (ko) | 2010-09-27 |
| CA2452768C (en) | 2011-06-07 |
| CN100347297C (zh) | 2007-11-07 |
| US20040197858A1 (en) | 2004-10-07 |
| JPWO2003004641A1 (ja) | 2004-10-28 |
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| Date | Code | Title | Description |
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| UEP | Publication of translation of european patent specification |
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