ATE433456T1 - Methoden zur reinigung von dns - Google Patents

Methoden zur reinigung von dns

Info

Publication number
ATE433456T1
ATE433456T1 AT00936329T AT00936329T ATE433456T1 AT E433456 T1 ATE433456 T1 AT E433456T1 AT 00936329 T AT00936329 T AT 00936329T AT 00936329 T AT00936329 T AT 00936329T AT E433456 T1 ATE433456 T1 AT E433456T1
Authority
AT
Austria
Prior art keywords
plasmid dna
methods
purification
aqueous solution
supercoiled
Prior art date
Application number
AT00936329T
Other languages
English (en)
Inventor
Natarajan Ramasubramanyan
Original Assignee
Lonza Biolog Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22474295&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE433456(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Lonza Biolog Inc filed Critical Lonza Biolog Inc
Application granted granted Critical
Publication of ATE433456T1 publication Critical patent/ATE433456T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT00936329T 1999-05-28 2000-05-26 Methoden zur reinigung von dns ATE433456T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13677299P 1999-05-28 1999-05-28
PCT/US2000/014527 WO2000073318A1 (en) 1999-05-28 2000-05-26 Methods of dna purification and purified dna

Publications (1)

Publication Number Publication Date
ATE433456T1 true ATE433456T1 (de) 2009-06-15

Family

ID=22474295

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00936329T ATE433456T1 (de) 1999-05-28 2000-05-26 Methoden zur reinigung von dns

Country Status (21)

Country Link
EP (1) EP1187840B2 (de)
JP (2) JP5320528B2 (de)
KR (1) KR100688349B1 (de)
CN (1) CN1264854C (de)
AT (1) ATE433456T1 (de)
AU (1) AU782153B2 (de)
BR (1) BR0011033A (de)
CA (1) CA2375215C (de)
CZ (1) CZ20014274A3 (de)
DE (1) DE60042366D1 (de)
DK (1) DK1187840T4 (de)
ES (1) ES2328008T5 (de)
HU (1) HUP0301869A2 (de)
IL (3) IL146695A0 (de)
MX (1) MXPA01012213A (de)
NO (1) NO20015703L (de)
NZ (1) NZ515645A (de)
PL (1) PL203013B1 (de)
RO (1) RO122855B1 (de)
RU (1) RU2001135838A (de)
WO (1) WO2000073318A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1264854C (zh) * 1999-05-28 2006-07-19 坎布雷科斯生物科学巴尔的摩公司 Dna纯化方法和纯化的dna
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
GB0127803D0 (en) * 2001-11-20 2002-01-09 Glaxo Group Ltd Processing nucleic acid
KR100463415B1 (ko) * 2001-12-12 2004-12-23 씨제이 주식회사 세균내ｄｎａ 또는 ｒｎａ로부터 리포폴리사카라이드를제거하는 방법
SE0200543D0 (sv) * 2002-02-21 2002-02-21 Amersham Biosciences Ab Method of separation using aromatic thioether ligands
US20040127648A1 (en) * 2002-12-31 2004-07-01 Ciphergen Biosystems, Inc. Sorbent and method for the separation of plasmid DNA
NZ545449A (en) * 2003-09-17 2009-07-31 Centelion Formerly Gencell Sas Device and Method of preparation of pharmaceutical grade plasmid DNA
EA010576B1 (ru) * 2004-04-19 2008-10-30 Сентельон Способ очистки плазмидной днк
KR100612869B1 (ko) * 2004-11-08 2006-08-14 삼성전자주식회사 고정화된 금속-리간드 복합체에 의한 세포 용해 방법
JP4811773B2 (ja) * 2005-06-08 2011-11-09 肇 柄谷 核酸の精製法
WO2007114762A1 (en) * 2006-03-31 2007-10-11 Ge Healthcare Bio-Sciences Ab Method of separation of deoxyribonucleic acids
US20120220729A1 (en) 2011-02-28 2012-08-30 Boehringer Ingelheim International Gmbh Liquid phase separation of plasmid dna isoforms and topoisomers
EP3054008A4 (de) 2013-10-03 2017-04-12 Kyowa Hakko Bio Co., Ltd. Verfahren zur reinigung einer doppelsträngigen ribonukleinsäure
BR112018075667B1 (pt) * 2016-06-14 2024-02-20 Biogen Ma, Inc Métodos para separar um oligonucleotídeo alvo de uma mistura contendo o oligonucleotídeo alvo e uma impureza relacionada com o produto
MX2020009579A (es) * 2018-03-15 2020-11-09 Intergalactic Therapeutics Inc Vectores de adn sintetico y metodos de uso.
US11865480B2 (en) 2019-05-07 2024-01-09 Electronics and Telecommunications Research Institute Cancer Rop Co., Ltd. Nucleic acid purification device and nucleic acid purification method
GB2700048B (en) 2019-09-18 2026-03-04 Aldevron Llc synthetic dna vectors and method of use
CN111721871A (zh) * 2020-06-24 2020-09-29 南京济群生物科技有限公司 一种质粒超螺旋dna含量的高分离度检测方法
KR20220018185A (ko) 2020-08-06 2022-02-15 주식회사 바이오솔루션 플라스미드 dna 추출방법
KR20220018187A (ko) 2020-08-06 2022-02-15 주식회사 바이오솔루션 플라스미드 dna 추출키트
EP4225462A1 (de) * 2020-10-05 2023-08-16 Sartorius BIA Separations d.o.o. Verbesserte entfernung von rna und verunreinigungen aus dna-plasmidzubereitungen durch hydrophobe interaktionschromatographie

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5622960A (en) * 1992-04-14 1997-04-22 The United States Of America As Represented By The Department Of Health And Human Services Topoisomerase II inhibitors and therapeutic uses therefor
US5561064A (en) 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
DE59507433D1 (de) 1994-02-07 2000-01-20 Qiagen Gmbh Verfahren zur herstellung endotoxinfreier oder an endotoxin abgereicherter nucleinsäuren und/oder oligonucleotide für die gentherapie
US5707850A (en) 1995-11-09 1998-01-13 Cole; Kenneth D. Concentration and size-fractionation of nucleic acids and viruses in porous media
US5707812A (en) 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
DE69922740T2 (de) * 1998-05-11 2005-12-08 Tosoh Corp., Shinnanyo Methode zur Trennung von Nucleinsäuren mittels Flüssigchromatographie
JP2000035423A (ja) * 1998-05-11 2000-02-02 Tosoh Corp 液体クロマトグラフィ―による核酸の分離法
CN1264854C (zh) * 1999-05-28 2006-07-19 坎布雷科斯生物科学巴尔的摩公司 Dna纯化方法和纯化的dna

Also Published As

Publication number Publication date
IL146695A0 (en) 2002-07-25
JP5320528B2 (ja) 2013-10-23
IL222755A (en) 2015-08-31
PL203013B1 (pl) 2009-08-31
DE60042366D1 (de) 2009-07-23
ES2328008T5 (es) 2013-02-25
NO20015703L (no) 2002-01-03
EP1187840B2 (de) 2012-12-19
JP2012050463A (ja) 2012-03-15
RU2001135838A (ru) 2004-01-20
JP2003524405A (ja) 2003-08-19
BR0011033A (pt) 2003-07-01
EP1187840B1 (de) 2009-06-10
CA2375215A1 (en) 2000-12-07
IL222755A0 (en) 2012-12-31
EP1187840A4 (de) 2002-09-18
CZ20014274A3 (cs) 2003-01-15
CN1264854C (zh) 2006-07-19
KR20020092778A (ko) 2002-12-12
ES2328008T3 (es) 2009-11-06
MXPA01012213A (es) 2003-06-24
AU5165500A (en) 2000-12-18
IL146695A (en) 2012-12-31
DK1187840T3 (da) 2009-10-19
WO2000073318A1 (en) 2000-12-07
KR100688349B1 (ko) 2007-02-28
CN1390229A (zh) 2003-01-08
AU782153B2 (en) 2005-07-07
NO20015703D0 (no) 2001-11-22
RO122855B1 (ro) 2010-03-30
DK1187840T4 (da) 2013-02-04
EP1187840A1 (de) 2002-03-20
PL364051A1 (en) 2004-12-13
CA2375215C (en) 2012-11-20
NZ515645A (en) 2004-07-30
HUP0301869A2 (hu) 2003-08-28

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