ES2196870T3 - Procedimiento para la purificacion o aislamiento de acidos nucleicos virales. - Google Patents
Procedimiento para la purificacion o aislamiento de acidos nucleicos virales.Info
- Publication number
- ES2196870T3 ES2196870T3 ES99950544T ES99950544T ES2196870T3 ES 2196870 T3 ES2196870 T3 ES 2196870T3 ES 99950544 T ES99950544 T ES 99950544T ES 99950544 T ES99950544 T ES 99950544T ES 2196870 T3 ES2196870 T3 ES 2196870T3
- Authority
- ES
- Spain
- Prior art keywords
- nucleic acids
- isolation
- purification
- procedure
- viral nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Procedimiento para la purificación y el aislamiento de ácidos nucleicos de partículas de virus presentes en material de muestras biológico, caracterizado porque a) se adiciona a una muestra que contiene virus polietilenglicol y cloruro sódico y las partículas de virus son precipitadas en frío a una temperatura de aproximadamente 4°C, b) se lleva a cabo una centrifugación y c) los pellets obtenidos se disuelven en un tampón de isotiocianato de guanidina, d) a la mezcla obtenida en la etapa c) se adiciona etanol, y e) se lleva a cabo una purificación en una matriz de sílice, estando los ácidos nucleicos purificados a continuación presentes en el eluato.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19856415A DE19856415C2 (de) | 1998-12-08 | 1998-12-08 | Verfahren zur Reinigung bzw. Isolierung viraler Nukleinsäuren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2196870T3 true ES2196870T3 (es) | 2003-12-16 |
Family
ID=7890263
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES99950544T Expired - Lifetime ES2196870T3 (es) | 1998-12-08 | 1999-09-23 | Procedimiento para la purificacion o aislamiento de acidos nucleicos virales. |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1137800B1 (es) |
| AT (1) | ATE236999T1 (es) |
| AU (1) | AU6329099A (es) |
| DE (2) | DE19856415C2 (es) |
| ES (1) | ES2196870T3 (es) |
| HK (1) | HK1042116A1 (es) |
| WO (1) | WO2000034510A1 (es) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10006662A1 (de) | 2000-02-15 | 2001-08-23 | Antigen Produktions Gmbh | Gefäß zur Nukleinsäureanalytik |
| ES2314646T3 (es) | 2004-03-22 | 2009-03-16 | Solvay Pharmaceuticals Gmbh | Composiciones farmaceuticas por via de productos que contienen lipasa , en particular de pancreatina, que contienen tensioactivos. |
| HUE031042T2 (en) | 2005-07-29 | 2017-06-28 | Abbott Laboratories Gmbh | Methods for producing pancreatin powder with low viral content |
| US11266607B2 (en) | 2005-08-15 | 2022-03-08 | AbbVie Pharmaceuticals GmbH | Process for the manufacture and use of pancreatin micropellet cores |
| US9198871B2 (en) | 2005-08-15 | 2015-12-01 | Abbott Products Gmbh | Delayed release pancreatin compositions |
| US10072256B2 (en) | 2006-05-22 | 2018-09-11 | Abbott Products Gmbh | Process for separating and determining the viral load in a pancreatin sample |
| AU2007253380B2 (en) * | 2006-05-22 | 2013-03-14 | Abbott Laboratories Gmbh | Process for separating and determining the viral load in a pancreatin sample |
| DE102008023297B4 (de) | 2008-05-08 | 2014-06-26 | Aj Innuscreen Gmbh | Verfahren zur Anreicherung und Isolierung von Nukleinsäuren oder Viren |
| DE102015215894B4 (de) | 2015-03-26 | 2017-11-30 | Aj Innuscreen Gmbh | Verfahren zur Anreicherung von Biomolekülen und zur Entfernung der Biomoleküle aus einer biologischen Probe |
| PL3487869T3 (pl) | 2016-07-25 | 2024-03-18 | Ist Innuscreen Gmbh | Sposób wzbogacania biomolekuł i usuwania biomolekuł z próbki biologicznej |
| CN111850171A (zh) * | 2020-08-03 | 2020-10-30 | 浙江大学 | 一种深海沉积物样品中慢病毒的检测方法 |
| DE102021104319A1 (de) | 2021-02-23 | 2022-08-25 | Aj Innuscreen Gmbh | Verfahren zur simultanen Isolierung von Nukleinsäuren aus sedimentierbaren und nicht sedimentierbaren Biomolekülen in Wasserproben |
| CN115505650A (zh) * | 2022-10-20 | 2022-12-23 | 江苏省农业科学院 | 一种基于快速沉降病毒直接pcr检测植物病毒的方法 |
-
1998
- 1998-12-08 DE DE19856415A patent/DE19856415C2/de not_active Expired - Fee Related
-
1999
- 1999-09-23 ES ES99950544T patent/ES2196870T3/es not_active Expired - Lifetime
- 1999-09-23 EP EP99950544A patent/EP1137800B1/de not_active Expired - Lifetime
- 1999-09-23 WO PCT/EP1999/007093 patent/WO2000034510A1/de not_active Ceased
- 1999-09-23 AU AU63290/99A patent/AU6329099A/en not_active Abandoned
- 1999-09-23 AT AT99950544T patent/ATE236999T1/de not_active IP Right Cessation
- 1999-09-23 HK HK02102445.5A patent/HK1042116A1/zh unknown
- 1999-09-23 DE DE59905002T patent/DE59905002D1/de not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| AU6329099A (en) | 2000-06-26 |
| DE59905002D1 (de) | 2003-05-15 |
| HK1042116A1 (zh) | 2002-08-02 |
| DE19856415C2 (de) | 2001-06-07 |
| EP1137800B1 (de) | 2003-04-09 |
| WO2000034510A1 (de) | 2000-06-15 |
| EP1137800A1 (de) | 2001-10-04 |
| DE19856415A1 (de) | 2000-06-15 |
| ATE236999T1 (de) | 2003-04-15 |
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