ATE437225T1 - Verfahren zur polynukleotidsynthese - Google Patents

Verfahren zur polynukleotidsynthese

Info

Publication number
ATE437225T1
ATE437225T1 AT01967728T AT01967728T ATE437225T1 AT E437225 T1 ATE437225 T1 AT E437225T1 AT 01967728 T AT01967728 T AT 01967728T AT 01967728 T AT01967728 T AT 01967728T AT E437225 T1 ATE437225 T1 AT E437225T1
Authority
AT
Austria
Prior art keywords
polynucleotide synthesis
loops
polynucleotides
isothermal
synthesis
Prior art date
Application number
AT01967728T
Other languages
English (en)
Inventor
Kentaro Nagamine
Original Assignee
Eiken Chemical
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=18768163&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE437225(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Eiken Chemical filed Critical Eiken Chemical
Application granted granted Critical
Publication of ATE437225T1 publication Critical patent/ATE437225T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT01967728T 2000-09-19 2001-09-19 Verfahren zur polynukleotidsynthese ATE437225T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000283862 2000-09-19
PCT/JP2001/008142 WO2002024902A1 (en) 2000-09-19 2001-09-19 Method of synthesizing polynucleotide

Publications (1)

Publication Number Publication Date
ATE437225T1 true ATE437225T1 (de) 2009-08-15

Family

ID=18768163

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01967728T ATE437225T1 (de) 2000-09-19 2001-09-19 Verfahren zur polynukleotidsynthese

Country Status (9)

Country Link
US (3) US7374913B2 (de)
EP (2) EP2031058B1 (de)
JP (2) JP4918210B2 (de)
CN (1) CN1222614C (de)
AT (1) ATE437225T1 (de)
AU (1) AU2001288065A1 (de)
DE (1) DE60139331D1 (de)
ES (2) ES2327712T3 (de)
WO (1) WO2002024902A1 (de)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8445664B2 (en) * 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
ES2327712T3 (es) * 2000-09-19 2009-11-03 Eiken Kagaku Kabushiki Kaisha Procedimiento para sintetizar polinucleotidos.
AU2003211947A1 (en) 2002-02-20 2003-09-09 Sysmex Corporation PRIMERS FOR NUCLEIC ACID AMPLIFICATION IN DETECTING HOUSEKEEPING GENE mRNA AND TEST METHOD USING THESE PRIMERS
EP1510587B1 (de) 2002-05-21 2011-07-13 Sysmex Corporation Nukleinsäureamplifikationsprimer zum nachweis von cytokeratinen und untersuchungsverfahren mit verwendung der primer
AU2003280603A1 (en) * 2002-10-29 2004-05-25 Kabushiki Kaisha Dnaform Method of amplifying nucleic acid
EP1642978A4 (de) 2003-06-27 2007-03-21 Eiken Chemical Verfahren zum nachweis des sars-coronavirus
JP2005110587A (ja) * 2003-10-08 2005-04-28 Sapporo Breweries Ltd アリサイクロバチラス属菌検出用プライマー
TW200525026A (en) 2003-12-25 2005-08-01 Riken Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same
JP2008000002A (ja) * 2004-09-30 2008-01-10 Sysmex Corp リブロース2リン酸カルボキシラーゼスモールチェーン1A(RBCS−1A)遺伝子及び/又は該遺伝子のmRNAを検出するための核酸増幅用プライマ、及び内部標準として該遺伝子及び/又は該遺伝子のmRNAを用いた検査方法。
WO2006049061A1 (ja) 2004-11-01 2006-05-11 Eiken Kagaku Kabushiki Kaisha H5型又はh7型トリインフルエンザウイルスの検出方法
JP2006254784A (ja) * 2005-03-17 2006-09-28 Institute Of Physical & Chemical Research プライマーダイマーからの非特異的増幅を減少させる方法
FR2885140B1 (fr) 2005-04-29 2010-12-31 Millipore Corp Procede de detection et de caracterisation de microorgarnismes sur une membrane.
US8956850B2 (en) * 2008-06-05 2015-02-17 Butamax Advanced Biofuels Llc Enhanced pyruvate to acetolactate conversion in yeast
WO2007069331A1 (ja) * 2005-12-16 2007-06-21 Eiken Kagaku Kabushiki Kaisha ヒト免疫不全ウイルス1型の検出方法
JP4580875B2 (ja) * 2006-01-20 2010-11-17 株式会社東芝 核酸検出法のためのプライマーの設計方法
JP4881623B2 (ja) 2006-01-27 2012-02-22 シスメックス株式会社 Cea核酸増幅用プライマー、プライマーセット、及びがんの診断支援方法
CA2673772A1 (en) * 2007-01-17 2008-07-24 Meridian Bioscience, Inc. Stable reagents and kits useful in loop-mediated isothermal amplification (lamp)
CA2622649C (en) 2007-03-09 2018-04-24 Riken Nucleic acid amplification method using primer exhibiting exciton effect
JP4410268B2 (ja) 2007-03-28 2010-02-03 株式会社東芝 メチレンテトラヒドロ葉酸還元酵素(mthfr)の遺伝子型を検出するための核酸プライマーセット及び核酸プローブ
JP4410269B2 (ja) 2007-03-28 2010-02-03 株式会社東芝 N−アセチルトランスフェラーゼ2(nat2)の遺伝子型を検出するための核酸プライマーセット、キット、及び該プライマーセットを用いた検出方法
JP4490988B2 (ja) 2007-04-26 2010-06-30 株式会社東芝 血清アミロイドa1(saa1)の遺伝子型を検出するための核酸プライマーセット、キット、及び該プライマーセットを用いた検出方法
JP4450867B2 (ja) 2007-10-25 2010-04-14 独立行政法人理化学研究所 等温増幅方法およびそれに用いる等温増幅用キット
WO2009119331A1 (ja) * 2008-03-28 2009-10-01 シスメックス株式会社 サイトケラチン7のmRNAの検出用プライマを含む試薬
CN102105584B (zh) 2008-05-28 2014-05-14 花王株式会社 耐热菌类的检测方法
EP2325309B1 (de) 2008-05-29 2014-10-22 Kao Corporation Verfahren zum nachweis von paecilomyces variotii
EP2371951A4 (de) 2008-11-27 2012-07-18 Riken NEUARTIGES MutS-PROTEIN UND SEINE VERWENDUNG ZUR BESTIMMUNG VON MUTATIONEN
JPWO2010074265A1 (ja) 2008-12-25 2012-06-21 ユニバーサル・バイオ・リサーチ株式会社 検体の前処理方法、および生体関連物質の測定方法
GB0910302D0 (en) * 2009-06-15 2009-07-29 Lumora Ltd Nucleic acid amplification
EP2746395B1 (de) 2011-09-08 2017-11-29 Kabushiki Kaisha DNAFORM Primer-set, verfahren zur amplifizierung einer nukleinsäuresequenz damit und verfahren zur erkennung von mutierter nukleinsäure damit
JP6085564B2 (ja) 2011-10-31 2017-02-22 栄研化学株式会社 標的核酸の検出法
CA2892818A1 (en) 2012-11-28 2014-06-05 Abwiz Bio, Inc. Preparation of gene-specific templates for the use in single primer amplification
WO2014151177A1 (en) 2013-03-15 2014-09-25 3M Innovative Properties Company Sample concentrator and method of use
US10351918B2 (en) 2013-10-30 2019-07-16 Green Life Biotech, Llc Pathogenesis quantification systems and treatment methods for citrus greening blight
WO2016011280A1 (en) 2014-07-16 2016-01-21 Tangen Biosciences, Inc. Isothermal methods for amplifying nucleic acid samples
US11559801B2 (en) 2014-11-03 2023-01-24 Tangen Biosciences, Inc. Apparatus and method for cell, spore, or virus capture and disruption
JP6605238B2 (ja) * 2014-12-19 2019-11-13 国立大学法人 長崎大学 ザイールエボラウイルス検出用プライマーセット、アッセイキットおよび増幅方法
WO2016098892A1 (ja) * 2014-12-19 2016-06-23 国立大学法人 長崎大学 ザイールエボラウイルス検出用プライマーセット、アッセイキットおよび増幅方法
BR112017020690A2 (pt) 2015-03-30 2018-06-26 Greenlight Biosciences Inc produção livre de células de ácido ribonucleico
JPWO2017212904A1 (ja) * 2016-06-06 2019-04-11 国立大学法人 宮崎大学 複数のプライマーセットを組み合わせたlamp法を用いたアフリカ豚コレラウイルスの迅速検出法
WO2018209092A1 (en) * 2017-05-10 2018-11-15 Board Of Regents, The University Of Texas System Methods and devices related to amplifying nucleic acid at a variety of temperatures
JP7176682B2 (ja) 2018-02-22 2022-11-22 佐藤製薬株式会社 白癬菌の遺伝子をlamp法により検出するためのプライマーセット及びそれを含むキット並びにそれらを用いて白癬菌を検出する方法
CN109658982B (zh) * 2018-12-25 2020-06-19 人和未来生物科技(长沙)有限公司 一种用于基因测序的引物设计方法及系统
US12590324B2 (en) * 2019-01-14 2026-03-31 Camena Bioscience Limited Compositions and methods for template-free geometric enzymatic nucleic acid synthesis
US12325883B2 (en) 2019-01-15 2025-06-10 Neogen Food Safety Us Holdco Corporation Loop-mediated isothermal amplification primers for Shiga toxin-producing E. coli (STEC) detection
SG10201910254YA (en) 2019-11-04 2021-06-29 Denka Company Ltd Nucleic Acid Detection Method Using Lamp And Probe Detection
CN110878367A (zh) * 2019-12-05 2020-03-13 华南农业大学 一种可检测snp的新型cpa方法、引物组和试剂盒
JP7048819B2 (ja) 2020-02-27 2022-04-05 栄研化学株式会社 一本鎖rnaウイルスを検出する方法
CN115176036A (zh) 2020-03-05 2022-10-11 荣研化学株式会社 冠状病毒(SARS-CoV-2)的检测方法
AU2021236383A1 (en) 2020-03-13 2022-09-22 Uh-Oh Labs Inc. Looped primer and loop-de-loop method for detecting target nucleic acid
WO2021224355A1 (en) 2020-05-05 2021-11-11 Genomtec Sa Diagnostic primers, kits and methods for viral detection
EP4165218A1 (de) 2020-06-12 2023-04-19 Sherlock Biosciences, Inc. Sars-cov-2-detektion auf crispr-basis
CN116075596A (zh) 2020-08-07 2023-05-05 牛津纳米孔科技公开有限公司 鉴定核酸条形码的方法
CN112553300A (zh) * 2020-10-15 2021-03-26 德歌生物技术(山东)有限公司 引物设计方法、引物及等温扩增核酸片段的方法
EP3992300A1 (de) 2020-10-30 2022-05-04 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk Onderzoek TNO Probenvorbehandlung zur isothermen amplifikation
KR20230169935A (ko) 2021-01-12 2023-12-18 데피니티브 바이오테크놀로지스 엘엘씨 생물학적 시료들에서 핵산을 검출하기 위한 디바이스및 방법
WO2022173710A1 (en) 2021-02-09 2022-08-18 Sherlock Biosciences, Inc. Nucleic acid amplification using promoter primers
US11788129B2 (en) 2021-02-23 2023-10-17 Industrial Technology Research Institute Nucleic acid detection kit and nucleic acid detection method
PL437280A1 (pl) 2021-03-12 2022-09-19 Genomtec Spółka Akcyjna Zestaw starterów do powielania, sposób wykrywania infekcji bakteryjnej przenoszonej drogą płciową oraz zestaw do wykrywania infekcji
PL437660A1 (pl) 2021-04-22 2022-10-24 Genomtec Spółka Akcyjna Zestaw starterów, skład reagentów oraz metoda wykrywania bakterii Staphylococcus aureus opornej na metycylinę (MRSA)
WO2022226870A1 (zh) * 2021-04-29 2022-11-03 中国科学院大学宁波生命与健康产业研究院 恒温条件下合成核酸的方法及试剂盒和应用
CN113201583B (zh) * 2021-04-29 2022-02-08 国科宁波生命与健康产业研究院 恒温条件下合成核酸的方法及试剂盒和应用
JP2025500496A (ja) 2021-12-24 2025-01-09 エフ. ホフマン-ラ ロシュ アーゲー Lamp増幅産物の改良された検出
WO2025009968A1 (en) 2023-07-03 2025-01-09 Erasmus University Medical Center Rotterdam Methods for specific quantitation of the inducible hiv reservoir
EP4488391A1 (de) 2023-07-04 2025-01-08 Nederlandse Organisatie voor toegepast-natuurwetenschappelijk Onderzoek TNO Verfahren und mittel zum nachweis von legionella

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6410278B1 (en) 1998-11-09 2002-06-25 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid
WO2001034838A1 (en) 1999-11-08 2001-05-17 Eiken Kagaku Kabushiki Kaisha Method of detecting variation or polymorphism
KR100612551B1 (ko) 1999-11-08 2006-08-11 에이켄 카가꾸 가부시끼가이샤 핵산의 합성방법
EP2253717B1 (de) * 2000-04-07 2014-04-30 Eiken Kagaku Kabushiki Kaisha Methode zur Amplifizierung von Nukleinsäuren mit Hilfe von dopplesträngigen Nukleinsäuren als Templat
ES2327712T3 (es) * 2000-09-19 2009-11-03 Eiken Kagaku Kabushiki Kaisha Procedimiento para sintetizar polinucleotidos.

Also Published As

Publication number Publication date
US20040038253A1 (en) 2004-02-26
EP1327679A4 (de) 2005-06-01
JP4918210B2 (ja) 2012-04-18
US7846695B2 (en) 2010-12-07
EP2031058A1 (de) 2009-03-04
EP1327679B1 (de) 2009-07-22
US7374913B2 (en) 2008-05-20
ES2327712T3 (es) 2009-11-03
US20080213842A1 (en) 2008-09-04
EP1327679A1 (de) 2003-07-16
US20080213790A1 (en) 2008-09-04
US7851186B2 (en) 2010-12-14
JPWO2002024902A1 (ja) 2004-01-29
AU2001288065A1 (en) 2002-04-02
WO2002024902A1 (en) 2002-03-28
CN1474870A (zh) 2004-02-11
CN1222614C (zh) 2005-10-12
ES2390242T3 (es) 2012-11-07
DE60139331D1 (de) 2009-09-03
JP2012085652A (ja) 2012-05-10
HK1055445A1 (en) 2004-01-09
EP2031058B1 (de) 2012-07-18

Similar Documents

Publication Publication Date Title
DE60139331D1 (de) Verfahren zur polynukleotidsynthese
DE60020340D1 (de) Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür
DK1828381T3 (da) Templatedirigeret split-and-mix-syntese af småmolekylebiblioteker
DE69940623D1 (de) Prozess zur Synthetisierung von Nukleinsäure
ATE264268T1 (de) Verfahren zur herstellung von zsm-5 und zsm-11
UA83910C2 (ru) Способ получения 5-гидрокси-4-тиометилпиразольного соединения
ATE221893T1 (de) Verfahren zur herstellung von acylphosphinen und derivaten
ATE517857T1 (de) Herstellung von 4-phenylbutansäure
ATE323703T1 (de) Synthese von verbindungen die zur herstellung von ketorolac dienen
ATE318803T1 (de) Verfahren zur herstellung von laktonen
ATE525341T1 (de) Verfahren zur herstellung von 1-acetyl-1- chlorcyclopropan
DE602004012151D1 (de) Verfahren zur herstellung von chlorsulfonylisocyanat
HUP0100943A2 (hu) Eljárás poliamidok előállítására
DE3886501D1 (de) Verfahren zur Synthese von N,N-Dialkylhydroxylaminen.
ATE46190T1 (de) Gerbstoff und verfahren zu seiner herstellung.
ATE469141T1 (de) Verfahren zur herstellung von substituierten thiophensulfonylisocyanaten
ATE384730T1 (de) Verfahren zur herstellung von palladium(0)- haltigen verbindungen
DE60230366D1 (de) Verfahren zur Herstellung eines optisch aktiven Amids ausgehend von einem alpha, beta-ungesättigtem Amid Derivat in Anwesenheit eines optisch aktiven Übergangsmetallkomplexes, der eine Phosphin-Phosphoran Verbindung und Rhodium enthält
DE60219341D1 (de) Verfahren zur hydrierung von aceton
DE60203187D1 (de) Verfahren zur herstellung von di tert.-butyl-cis-1-propenylphosphonat
DE69812858D1 (de) Verfahren zur Herstellung von (R)-3-Hydroxy-4-Butyrolacton, nützlich für die Herstellung von (R)-Carnitin
DE60218533D1 (de) Nukleotidsequenzen mit aktivität zur steuerung der translationseffizienz und deren nutzung
ATE527213T1 (de) Verfahren zur herstellung von ammoniumpolythiomolybdat
DE60230740D1 (de) Unkatalysierte additionsreaktionen
DE602005004834D1 (de) Ein verbessertes Verfahren für die Synthese von Methylphenidaten und Zwischenprodukten

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties