ATE444370T1 - Expressionsvektor zur sekretion eines antikörperfragments unter verwendung einer e.coli-signalsequenz sowie verfahren zur massenproduktion des antikörperfragments - Google Patents

Expressionsvektor zur sekretion eines antikörperfragments unter verwendung einer e.coli-signalsequenz sowie verfahren zur massenproduktion des antikörperfragments

Info

Publication number
ATE444370T1
ATE444370T1 AT04793487T AT04793487T ATE444370T1 AT E444370 T1 ATE444370 T1 AT E444370T1 AT 04793487 T AT04793487 T AT 04793487T AT 04793487 T AT04793487 T AT 04793487T AT E444370 T1 ATE444370 T1 AT E444370T1
Authority
AT
Austria
Prior art keywords
antibody fragment
expression vector
signal sequence
secretion
mass production
Prior art date
Application number
AT04793487T
Other languages
English (en)
Inventor
Sung Youb Jung
Jin Sun Kim
Se Chang Kwon
Gwan Sun Lee
Original Assignee
Hanmi Pharm Ind Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hanmi Pharm Ind Co Ltd filed Critical Hanmi Pharm Ind Co Ltd
Application granted granted Critical
Publication of ATE444370T1 publication Critical patent/ATE444370T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT04793487T 2003-10-16 2004-10-14 Expressionsvektor zur sekretion eines antikörperfragments unter verwendung einer e.coli-signalsequenz sowie verfahren zur massenproduktion des antikörperfragments ATE444370T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020030072216A KR100570422B1 (ko) 2003-10-16 2003-10-16 대장균 분비서열을 이용하여 항체 단편을 분비·생산하는 발현벡터 및 이를 이용하여 항체 단편을 대량 생산하는 방법
PCT/KR2004/002625 WO2005038031A1 (en) 2003-10-16 2004-10-14 Expression vector for secreting antibody fragment using e. coli signal sequence and method for mass-producing antibody fragment

Publications (1)

Publication Number Publication Date
ATE444370T1 true ATE444370T1 (de) 2009-10-15

Family

ID=36569730

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04793487T ATE444370T1 (de) 2003-10-16 2004-10-14 Expressionsvektor zur sekretion eines antikörperfragments unter verwendung einer e.coli-signalsequenz sowie verfahren zur massenproduktion des antikörperfragments

Country Status (8)

Country Link
US (1) US7718399B2 (de)
EP (1) EP1678308B1 (de)
JP (1) JP4448138B2 (de)
KR (1) KR100570422B1 (de)
AT (1) ATE444370T1 (de)
DE (1) DE602004023408D1 (de)
ES (1) ES2331573T3 (de)
WO (1) WO2005038031A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101323563B1 (ko) * 2005-10-18 2013-10-29 니토 보세키 가부시기가이샤 항체를 생산하는 트랜스제닉 누에와 그의 제조방법
DE102006004871A1 (de) * 2006-02-02 2007-08-09 Wacker Chemie Ag Mikroorganismenstamm zur Produktion von rekombinanten Proteinen
EP2001907A2 (de) * 2006-03-21 2008-12-17 Wyeth a Corporation of the State of Delaware Verfahren und zusammensetzungen für rage-antagonismen
DK1903115T3 (da) * 2006-09-22 2011-05-23 Wacker Chemie Ag Fremgangsmåde til fermentativ fremstilling af antistoffer
SI2496601T1 (sl) 2009-11-05 2017-09-29 F. Hoffmann-La Roche Ag Tehnike in sestavek za sekrecijo heterolognih polipeptidov
JP6362543B2 (ja) 2012-02-23 2018-07-25 ジュノ セラピューティクス ゲーエムベーハー 細胞および他の複雑な生物学的材料のクロマトグラフィーによる単離
PL3116999T3 (pl) 2014-03-14 2021-12-27 F.Hoffmann-La Roche Ag Sposoby i kompozycje do wydzielania polipeptydów heterologicznych
EP3122782A4 (de) 2014-03-27 2017-09-13 Dyax Corp. Zusammensetzungen und verfahren zur behandlung von diabetischem makulaödem
HK1258932A1 (zh) 2015-10-22 2019-11-22 Juno Therapeutics Gmbh 用於转导的方法、反应剂盒、反应剂和设备
CN108474791B (zh) 2015-10-22 2022-07-08 朱诺治疗学有限公司 培养细胞的方法及用于该方法的试剂盒和设备
MA49288A (fr) 2017-04-27 2020-03-04 Juno Therapeutics Gmbh Reactifs particulaires oligomères et leurs méthodes d'utilisation
EP3874024A1 (de) 2018-10-31 2021-09-08 Juno Therapeutics GmbH Verfahren zur selektion und stimulation von zellen und vorrichtung dafür
CN118272405B (zh) * 2024-02-28 2025-01-07 浙江大学杭州国际科创中心 一种高效分泌纳米抗体药物的基因工程菌及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2116774C (en) * 1991-09-19 2003-11-11 Paul J. Carter Expression in e. coli antibody fragments having at least a cysteine present as a free thiol. use for the production of bifunctional f(ab') 2 antibodies
ATE335072T1 (de) * 1993-05-28 2006-08-15 Scripps Research Inst Methoden für inhibition der cd14-abhängigen zellaktivierung
KR100316347B1 (ko) * 1998-09-15 2002-08-27 한미약품(주) 대장균엔테로톡신ⅱ신호펩티드의변형체와인체성장호르몬의융합단백질을발현하는재조합미생물및그를이용한인체성장호르몬의제조방법
GB0013810D0 (en) * 2000-06-06 2000-07-26 Celltech Chiroscience Ltd Biological products
CN100513414C (zh) * 2001-08-27 2009-07-15 杰南技术公司 进行抗体表达和装配的系统

Also Published As

Publication number Publication date
US20090104660A1 (en) 2009-04-23
KR20050036517A (ko) 2005-04-20
WO2005038031A1 (en) 2005-04-28
JP2007508032A (ja) 2007-04-05
EP1678308A4 (de) 2007-07-11
EP1678308A1 (de) 2006-07-12
KR100570422B1 (ko) 2006-04-11
ES2331573T3 (es) 2010-01-08
EP1678308B1 (de) 2009-09-30
JP4448138B2 (ja) 2010-04-07
US7718399B2 (en) 2010-05-18
DE602004023408D1 (de) 2009-11-12

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