ATE444370T1 - Expressionsvektor zur sekretion eines antikörperfragments unter verwendung einer e.coli-signalsequenz sowie verfahren zur massenproduktion des antikörperfragments - Google Patents

Expressionsvektor zur sekretion eines antikörperfragments unter verwendung einer e.coli-signalsequenz sowie verfahren zur massenproduktion des antikörperfragments

Info

Publication number
ATE444370T1
ATE444370T1 AT04793487T AT04793487T ATE444370T1 AT E444370 T1 ATE444370 T1 AT E444370T1 AT 04793487 T AT04793487 T AT 04793487T AT 04793487 T AT04793487 T AT 04793487T AT E444370 T1 ATE444370 T1 AT E444370T1
Authority
AT
Austria
Prior art keywords
antibody fragment
expression vector
signal sequence
secretion
mass production
Prior art date
Application number
AT04793487T
Other languages
English (en)
Inventor
Sung Youb Jung
Jin Sun Kim
Se Chang Kwon
Gwan Sun Lee
Original Assignee
Hanmi Pharm Ind Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hanmi Pharm Ind Co Ltd filed Critical Hanmi Pharm Ind Co Ltd
Application granted granted Critical
Publication of ATE444370T1 publication Critical patent/ATE444370T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT04793487T 2003-10-16 2004-10-14 Expressionsvektor zur sekretion eines antikörperfragments unter verwendung einer e.coli-signalsequenz sowie verfahren zur massenproduktion des antikörperfragments ATE444370T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020030072216A KR100570422B1 (ko) 2003-10-16 2003-10-16 대장균 분비서열을 이용하여 항체 단편을 분비·생산하는 발현벡터 및 이를 이용하여 항체 단편을 대량 생산하는 방법
PCT/KR2004/002625 WO2005038031A1 (en) 2003-10-16 2004-10-14 Expression vector for secreting antibody fragment using e. coli signal sequence and method for mass-producing antibody fragment

Publications (1)

Publication Number Publication Date
ATE444370T1 true ATE444370T1 (de) 2009-10-15

Family

ID=36569730

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04793487T ATE444370T1 (de) 2003-10-16 2004-10-14 Expressionsvektor zur sekretion eines antikörperfragments unter verwendung einer e.coli-signalsequenz sowie verfahren zur massenproduktion des antikörperfragments

Country Status (8)

Country Link
US (1) US7718399B2 (de)
EP (1) EP1678308B1 (de)
JP (1) JP4448138B2 (de)
KR (1) KR100570422B1 (de)
AT (1) ATE444370T1 (de)
DE (1) DE602004023408D1 (de)
ES (1) ES2331573T3 (de)
WO (1) WO2005038031A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101323563B1 (ko) * 2005-10-18 2013-10-29 니토 보세키 가부시기가이샤 항체를 생산하는 트랜스제닉 누에와 그의 제조방법
DE102006004871A1 (de) * 2006-02-02 2007-08-09 Wacker Chemie Ag Mikroorganismenstamm zur Produktion von rekombinanten Proteinen
MX2008011933A (es) * 2006-03-21 2008-12-18 Wyeth Corp Metodos y composiciones para antagonismo de rage.
DK1903115T3 (da) * 2006-09-22 2011-05-23 Wacker Chemie Ag Fremgangsmåde til fermentativ fremstilling af antistoffer
KR101968766B1 (ko) 2009-11-05 2019-04-12 제넨테크, 인크. 이종 폴리펩티드의 분비를 위한 방법 및 조성물
SG11201404991YA (en) 2012-02-23 2014-09-26 Stage Cell Therapeutics Gmbh Chromatographic isolation of cells and other complex biological materials
SG11201607519VA (en) * 2014-03-14 2016-10-28 Genentech Inc Methods and compositions for secretion of heterologous polypeptides
IL247942B (en) 2014-03-27 2022-09-01 Dyax Corp Compositions and methods for drops in macular edema as a result of diabetes
MA45489A (fr) 2015-10-22 2018-08-29 Juno Therapeutics Gmbh Procédés de culture de cellules, kits et appareil associés
RU2761555C2 (ru) 2015-10-22 2021-12-09 Джуно Терапьютикс Гмбх Способы, наборы, средства и устройства для трансдукции
AU2018260380B2 (en) 2017-04-27 2025-02-13 Juno Therapeutics Gmbh Oligomeric particle reagents and methods of use thereof
WO2020089343A1 (en) 2018-10-31 2020-05-07 Juno Therapeutics Gmbh Methods for selection and stimulation of cells and apparatus for same
CN118272405B (zh) * 2024-02-28 2025-01-07 浙江大学杭州国际科创中心 一种高效分泌纳米抗体药物的基因工程菌及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0604580A1 (de) * 1991-09-19 1994-07-06 Genentech, Inc. Expression in E.Coli von Antikörperfragmenten mit mindestens einem cystein in freier -SH Form. Anwendung in der Herstellung bifunktioneller Antikörper.
US5820858A (en) * 1993-05-28 1998-10-13 The Scripps Research Institute Methods and compositions for inhibiting CD14 mediated cell activation
KR100316347B1 (ko) * 1998-09-15 2002-08-27 한미약품(주) 대장균엔테로톡신ⅱ신호펩티드의변형체와인체성장호르몬의융합단백질을발현하는재조합미생물및그를이용한인체성장호르몬의제조방법
GB0013810D0 (en) * 2000-06-06 2000-07-26 Celltech Chiroscience Ltd Biological products
EP1427744B1 (de) * 2001-08-27 2007-12-26 Genentech, Inc. System zur antikörperexpression und- synthese

Also Published As

Publication number Publication date
KR100570422B1 (ko) 2006-04-11
US7718399B2 (en) 2010-05-18
US20090104660A1 (en) 2009-04-23
JP2007508032A (ja) 2007-04-05
ES2331573T3 (es) 2010-01-08
KR20050036517A (ko) 2005-04-20
WO2005038031A1 (en) 2005-04-28
EP1678308B1 (de) 2009-09-30
EP1678308A1 (de) 2006-07-12
EP1678308A4 (de) 2007-07-11
DE602004023408D1 (de) 2009-11-12
JP4448138B2 (ja) 2010-04-07

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