ATE260979T1 - Verfahren zur herstellung extrazellulärer proteine in bakterien - Google Patents
Verfahren zur herstellung extrazellulärer proteine in bakterienInfo
- Publication number
- ATE260979T1 ATE260979T1 AT95939231T AT95939231T ATE260979T1 AT E260979 T1 ATE260979 T1 AT E260979T1 AT 95939231 T AT95939231 T AT 95939231T AT 95939231 T AT95939231 T AT 95939231T AT E260979 T1 ATE260979 T1 AT E260979T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- prepropeptide
- sequence encoding
- cell membrane
- recombinant vector
- Prior art date
Links
- 241000894006 Bacteria Species 0.000 title abstract 2
- 108050001049 Extracellular proteins Proteins 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 2
- 101710089384 Extracellular protease Proteins 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 230000001580 bacterial effect Effects 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 2
- 210000000170 cell membrane Anatomy 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 101000793340 Achromobacter lyticus Protease 1 Proteins 0.000 abstract 1
- 102000005741 Metalloproteases Human genes 0.000 abstract 1
- 108010006035 Metalloproteases Proteins 0.000 abstract 1
- 102000012479 Serine Proteases Human genes 0.000 abstract 1
- 108010022999 Serine Proteases Proteins 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 239000002609 medium Substances 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/605—Glucagons
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/824—Achromobacter
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK141194 | 1994-12-09 | ||
| PCT/DK1995/000498 WO1996017943A1 (en) | 1994-12-09 | 1995-12-08 | A process of producing extracellular proteins in bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE260979T1 true ATE260979T1 (de) | 2004-03-15 |
Family
ID=8104544
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT95939231T ATE260979T1 (de) | 1994-12-09 | 1995-12-08 | Verfahren zur herstellung extrazellulärer proteine in bakterien |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US6171823B1 (de) |
| EP (1) | EP0797671B1 (de) |
| JP (1) | JP3920331B2 (de) |
| AT (1) | ATE260979T1 (de) |
| AU (1) | AU4114596A (de) |
| DE (1) | DE69532646T2 (de) |
| WO (1) | WO1996017943A1 (de) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0797671B1 (de) * | 1994-12-09 | 2004-03-03 | Novo Nordisk A/S | Verfahren zur herstellung extrazellulärer proteine in bakterien |
| DE59812768D1 (de) * | 1997-08-22 | 2005-06-09 | Roche Diagnostics Gmbh | Autokatalytisch aktivierbare zymogene vorstufen von proteasen und deren verwendung |
| CA2485695A1 (en) * | 2002-05-24 | 2003-12-04 | Fred W. Wagner | Method for universal enzymatic production of bioactive peptides |
| AU2003243316A1 (en) * | 2002-05-24 | 2003-12-12 | Nps Allelix Corp. | Method for enzymatic production of glp-2(1-33) and glp-2-(1-34) peptides |
| US9453251B2 (en) | 2002-10-08 | 2016-09-27 | Pfenex Inc. | Expression of mammalian proteins in Pseudomonas fluorescens |
| HRP20120315T1 (hr) * | 2003-11-21 | 2012-05-31 | Nps Pharmaceuticals | Proizvodnja peptida 2 nalik glukagonu i analoga |
| CA2546157C (en) | 2003-11-21 | 2014-07-22 | Dow Global Technolgies Inc. | Improved expression systems with sec-system secretion |
| BRPI0513826A2 (pt) | 2004-07-26 | 2010-06-22 | Dow Global Technologies Inc | processo para expressão de proteìna melhorada através de engenharia de cepa |
| EP2468869B1 (de) | 2007-01-31 | 2015-03-18 | Pfenex Inc. | Bakterielle Leader-Sequenzen für erhöhte Expression |
| US9580719B2 (en) | 2007-04-27 | 2017-02-28 | Pfenex, Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
| US9394571B2 (en) | 2007-04-27 | 2016-07-19 | Pfenex Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
| EP2611922A1 (de) * | 2010-08-31 | 2013-07-10 | Greenlight Biosciences, Inc. | Verfahren zur flusssteuerung bei stoffwechselwegen durch proteasemanipulation |
| DE102011008075A1 (de) * | 2011-01-07 | 2012-07-12 | Universität Bayreuth | Verfahren zur rekombinanten Herstellung von mehrfach Disulfid-verbrückten Peptiden |
| AU2014306074B2 (en) | 2013-08-05 | 2018-08-30 | Greenlight Biosciences, Inc. | Engineered proteins with a protease cleavage site |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5536661A (en) * | 1987-03-10 | 1996-07-16 | Novo Nordisk A/S | Process for the production of protein products in aspergillus |
| JP3205331B2 (ja) * | 1989-03-14 | 2001-09-04 | 和光純薬工業株式会社 | アクロモバクタープロテアーゼｉ類遺伝子及びその遺伝子産物 |
| US5296773A (en) * | 1993-04-20 | 1994-03-22 | General Motors Corporation | Composite rotor for a synchronous reluctance machine |
| DK52293D0 (de) * | 1993-05-05 | 1993-05-05 | Novo Nordisk As | |
| US5843753A (en) * | 1994-05-04 | 1998-12-01 | Novo Nordisk A/S | Metalloprotease having increased activity |
| EP0797671B1 (de) * | 1994-12-09 | 2004-03-03 | Novo Nordisk A/S | Verfahren zur herstellung extrazellulärer proteine in bakterien |
| AU2091397A (en) * | 1996-03-12 | 1997-10-01 | Novo Nordisk A/S | Novel achromobacter lyticus protease variants |
-
1995
- 1995-12-08 EP EP95939231A patent/EP0797671B1/de not_active Expired - Lifetime
- 1995-12-08 JP JP51726796A patent/JP3920331B2/ja not_active Expired - Lifetime
- 1995-12-08 US US08/849,602 patent/US6171823B1/en not_active Expired - Lifetime
- 1995-12-08 WO PCT/DK1995/000498 patent/WO1996017943A1/en not_active Ceased
- 1995-12-08 AU AU41145/96A patent/AU4114596A/en not_active Abandoned
- 1995-12-08 DE DE69532646T patent/DE69532646T2/de not_active Expired - Lifetime
- 1995-12-08 AT AT95939231T patent/ATE260979T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| WO1996017943A1 (en) | 1996-06-13 |
| JP3920331B2 (ja) | 2007-05-30 |
| AU4114596A (en) | 1996-06-26 |
| EP0797671B1 (de) | 2004-03-03 |
| DE69532646D1 (de) | 2004-04-08 |
| DE69532646T2 (de) | 2005-03-03 |
| US6171823B1 (en) | 2001-01-09 |
| EP0797671A1 (de) | 1997-10-01 |
| JPH10511845A (ja) | 1998-11-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |