ATE446512T1 - Doppeltes hybridsystem auf der basis von gen- silencing durch transkriptionelle interferenz - Google Patents

Doppeltes hybridsystem auf der basis von gen- silencing durch transkriptionelle interferenz

Info

Publication number
ATE446512T1
ATE446512T1 AT05812049T AT05812049T ATE446512T1 AT E446512 T1 ATE446512 T1 AT E446512T1 AT 05812049 T AT05812049 T AT 05812049T AT 05812049 T AT05812049 T AT 05812049T AT E446512 T1 ATE446512 T1 AT E446512T1
Authority
AT
Austria
Prior art keywords
protein
hybrid system
double hybrid
geni
silencing
Prior art date
Application number
AT05812049T
Other languages
English (en)
Inventor
Alain Fournier
Ralf Behrens
Silvio Bandiera
Original Assignee
Aventis Pharma Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Sa filed Critical Aventis Pharma Sa
Application granted granted Critical
Publication of ATE446512T1 publication Critical patent/ATE446512T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Optical Integrated Circuits (AREA)
  • Optical Fibers, Optical Fiber Cores, And Optical Fiber Bundles (AREA)
  • Curing Cements, Concrete, And Artificial Stone (AREA)
AT05812049T 2004-10-25 2005-10-24 Doppeltes hybridsystem auf der basis von gen- silencing durch transkriptionelle interferenz ATE446512T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0411372A FR2877010B1 (fr) 2004-10-25 2004-10-25 Systeme double-hybride base sur la mise sous silence de genes par interference transcriptionnelle
PCT/FR2005/002646 WO2006045941A1 (fr) 2004-10-25 2005-10-24 Systeme double-hybride base sur la mise sous silence de genes par interference transcriptionnelle.

Publications (1)

Publication Number Publication Date
ATE446512T1 true ATE446512T1 (de) 2009-11-15

Family

ID=34950754

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05812049T ATE446512T1 (de) 2004-10-25 2005-10-24 Doppeltes hybridsystem auf der basis von gen- silencing durch transkriptionelle interferenz

Country Status (16)

Country Link
US (1) US20090005253A1 (de)
EP (1) EP1807697B1 (de)
JP (1) JP2008517591A (de)
KR (1) KR20070084523A (de)
CN (1) CN101065668A (de)
AT (1) ATE446512T1 (de)
AU (1) AU2005298499A1 (de)
BR (1) BRPI0516765A (de)
CA (1) CA2583193A1 (de)
DE (1) DE602005017296D1 (de)
DK (1) DK1807697T3 (de)
FR (1) FR2877010B1 (de)
IL (1) IL182383A (de)
MX (1) MX2007004970A (de)
RU (1) RU2403287C2 (de)
WO (1) WO2006045941A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2529356C2 (ru) * 2012-09-27 2014-09-27 Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук Модифицированная дрожжевая двугибридная система для эффективного исследования взаимодействия между белками и их доменами.
SG11202103917VA (en) 2018-10-16 2021-05-28 Blueallele Llc Methods for targeted insertion of dna in genes
RU2762410C1 (ru) * 2021-02-25 2021-12-21 Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук (ИБГ РАН) Модификация дрожжевой двугибридной системы, которая позволяет тестировать взаимодействия между белками внутри многокомпонентных белковых комплексов
CN115992165A (zh) * 2022-03-14 2023-04-21 武汉合生科技有限公司 一种提升目标产物合成菌株生产性能的方法
WO2024137857A1 (en) * 2022-12-22 2024-06-27 Tevard Biosciences, Inc. Conditional expression of a gene of interest by convergent promoters and uses thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ306767A (en) * 1995-04-11 2000-03-27 Univ Johns Hopkins Method of identifying molecular interactions employing counterselection and at least two hybrid molecules or two hybrid systems
US5948620A (en) * 1996-08-05 1999-09-07 Amersham International Plc Reverse two-hybrid system employing post-translation signal modulation
US5885779A (en) * 1997-09-09 1999-03-23 University Of British Columbia Repressed trans-activator system for characterization of protein-protein interactions
WO2002101011A2 (en) * 2001-06-08 2002-12-19 The Penn State Research Foundation Reagents and methods for detection and characterization of protein-protein interactions

Also Published As

Publication number Publication date
EP1807697B1 (de) 2009-10-21
FR2877010B1 (fr) 2007-03-16
CA2583193A1 (fr) 2006-05-04
IL182383A (en) 2010-12-30
BRPI0516765A (pt) 2008-09-16
RU2007119387A (ru) 2008-11-27
JP2008517591A (ja) 2008-05-29
KR20070084523A (ko) 2007-08-24
AU2005298499A1 (en) 2006-05-04
MX2007004970A (es) 2007-06-14
DE602005017296D1 (de) 2009-12-03
IL182383A0 (en) 2007-07-24
CN101065668A (zh) 2007-10-31
US20090005253A1 (en) 2009-01-01
FR2877010A1 (fr) 2006-04-28
WO2006045941A1 (fr) 2006-05-04
RU2403287C2 (ru) 2010-11-10
EP1807697A1 (de) 2007-07-18
DK1807697T3 (da) 2010-03-08

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