ATE447043T1 - Verfahren und kits zur verbesserung der hybridisierungsraten von polynukleotiden - Google Patents

Verfahren und kits zur verbesserung der hybridisierungsraten von polynukleotiden

Info

Publication number
ATE447043T1
ATE447043T1 AT01991158T AT01991158T ATE447043T1 AT E447043 T1 ATE447043 T1 AT E447043T1 AT 01991158 T AT01991158 T AT 01991158T AT 01991158 T AT01991158 T AT 01991158T AT E447043 T1 ATE447043 T1 AT E447043T1
Authority
AT
Austria
Prior art keywords
kits
polynucleotide hybridization
hybridization rates
improving
polynucleotides
Prior art date
Application number
AT01991158T
Other languages
English (en)
Inventor
Michael Becker
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of ATE447043T1 publication Critical patent/ATE447043T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT01991158T 2000-12-14 2001-12-07 Verfahren und kits zur verbesserung der hybridisierungsraten von polynukleotiden ATE447043T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25553500P 2000-12-14 2000-12-14
PCT/US2001/048592 WO2002048404A2 (en) 2000-12-14 2001-12-07 Method and kit for enhancing the association rates of polynucleotides

Publications (1)

Publication Number Publication Date
ATE447043T1 true ATE447043T1 (de) 2009-11-15

Family

ID=22968766

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01991158T ATE447043T1 (de) 2000-12-14 2001-12-07 Verfahren und kits zur verbesserung der hybridisierungsraten von polynukleotiden

Country Status (8)

Country Link
US (2) US7374945B2 (de)
EP (1) EP1377679B1 (de)
JP (1) JP2004537263A (de)
AT (1) ATE447043T1 (de)
AU (2) AU2002230901B2 (de)
CA (1) CA2436857A1 (de)
DE (1) DE60140317D1 (de)
WO (1) WO2002048404A2 (de)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60228100D1 (de) * 2001-08-24 2008-09-18 Nof Corp Verfahren zur beurteilung einer fehlpaarung zwischen einzelsträngigen nukleinsäuremolekülen
US6919180B2 (en) * 2002-03-22 2005-07-19 Sigma Genosys, L.P. Hybridization rate enhancement for substrate-bound specific nucleic acid-binding agents
DE10237284A1 (de) * 2002-08-14 2004-03-04 Advalytix Ag Nukleinsäurenachweis
MXPA05013658A (es) * 2003-06-11 2006-03-02 Hybridon Inc Oligonucleotidos inmunomoduladores estabilizados.
WO2006063065A2 (en) * 2004-12-08 2006-06-15 Gen-Probe Incorporated Detection of nucleic acids from multiple types of human papillomaviruses
CA2584230C (en) * 2005-05-06 2012-11-27 Gen-Probe Incorporated Methods of nucleic acid target capture
US7488581B2 (en) * 2006-03-20 2009-02-10 Kabushiki Kaisha Toshiba Method for detecting a target nucleic acid sequence
EP2636757B1 (de) * 2008-05-27 2016-11-23 Dako Denmark A/S Zusammensetzungen und Verfahren zum Nachweis von chromosomalen Abweichungen mit neuen Hybridisierungspuffern
US9303287B2 (en) * 2009-02-26 2016-04-05 Dako Denmark A/S Compositions and methods for RNA hybridization applications
WO2013046033A1 (en) 2011-09-30 2013-04-04 Dako Denmark A/S Hybridization compositions and methods using formamide
EP3604555B1 (de) 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequenzierung durch strukturanordnung
US11118226B2 (en) 2011-10-21 2021-09-14 Agilent Technologies, Inc. Hybridization compositions and methods
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
EP4491741B1 (de) 2011-12-22 2025-10-15 President And Fellows Of Harvard College Zusammensetzungen und verfahren zum nachweis von analyten
WO2014163886A1 (en) 2013-03-12 2014-10-09 President And Fellows Of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
SG10201710030QA (en) 2013-06-04 2018-01-30 Harvard College Rna-guided transcriptional regulation
US10179932B2 (en) 2014-07-11 2019-01-15 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
US10093967B2 (en) * 2014-08-12 2018-10-09 The Regents Of The University Of Michigan Detection of nucleic acids
WO2017079406A1 (en) 2015-11-03 2017-05-11 President And Fellows Of Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
AU2017257624B2 (en) 2016-04-25 2023-05-25 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
GB2569252A (en) 2016-08-31 2019-06-12 Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
JP7239465B2 (ja) * 2016-08-31 2023-03-14 プレジデント アンド フェローズ オブ ハーバード カレッジ 蛍光ｉｎ ｓｉｔｕ配列決定による検出のための核酸配列ライブラリの作製法
US11185568B2 (en) 2017-04-14 2021-11-30 President And Fellows Of Harvard College Methods for generation of cell-derived microfilament network
WO2018203304A1 (en) 2017-05-05 2018-11-08 Scopis Gmbh Surgical navigation system
EP3836967A4 (de) 2018-07-30 2022-06-15 ReadCoor, LLC Verfahren und systeme zur probenverarbeitung oder -analyse
US12227812B2 (en) * 2018-08-01 2025-02-18 Gen-Probe Incorporated Compositions and methods for detecting nucleic acids of Epstein-Barr virus
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
CN109988842B (zh) * 2019-04-19 2022-12-06 上海吉玛制药技术有限公司 一种寡核苷酸标记探针原位检测pdl1的试剂组与应用
CN115362264A (zh) 2020-01-29 2022-11-18 10X基因组学有限公司 用于分析物检测的组合物和方法
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
EP4446435A4 (de) * 2021-12-10 2025-04-02 Nitto Boseki Co., Ltd. Verfahren zum nachweis von mutationen in der zielbasensequenz von nukleinsäure, verfahren zur selektiven hemmung der amplifikation von nukleinsäure und kit zur implementierung davon
JP7394301B2 (ja) * 2021-12-10 2023-12-08 日東紡績株式会社 二本鎖核酸の融解温度上昇化剤及びその用途

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5132207A (en) * 1984-07-05 1992-07-21 Gen-Probe Incorporated Accelerated nucleic acid reassociation method
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
JPS638396A (ja) * 1986-06-30 1988-01-14 Wakunaga Pharmaceut Co Ltd ポリ標識化オリゴヌクレオチド誘導体
JPH0660885B2 (ja) 1987-08-20 1994-08-10 富士写真フイルム株式会社 電気泳動用媒体の製造方法
US4834854A (en) * 1987-08-20 1989-05-30 Fuji Photo Film Co., Ltd. Method of making an electrophoresis medium
US5283174A (en) * 1987-09-21 1994-02-01 Gen-Probe, Incorporated Homogenous protection assay
JPH081095Y2 (ja) 1987-09-22 1996-01-17 富士ゼロックス株式会社 画像記録装置
US5753437A (en) * 1987-10-13 1998-05-19 The United States Of America As Represented By The Department Of Health & Human Services Method of diagnosing cancer susceptibility or metastatic potential
US5589335A (en) * 1989-04-05 1996-12-31 Amoco Corporation Hybridization promotion reagents
US5474911A (en) * 1989-12-01 1995-12-12 The Board Of Trustees Of Leland Stanford Jr. University Promotion of high specificity molecular assembly
US5200314A (en) 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
GB9114180D0 (en) 1991-07-01 1991-08-21 Amersham Int Plc Enhancement of polynucleotide hybridisation
US6030954A (en) * 1991-09-05 2000-02-29 University Of Connecticut Targeted delivery of poly- or oligonucleotides to cells
WO1995006056A1 (en) * 1993-08-20 1995-03-02 University Of Medicine & Dentistry Of New Jersey Bridged polycationic polymer-oligonucleotide conjugates and methods for preparing same
US6045996A (en) * 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US5731148A (en) * 1995-06-07 1998-03-24 Gen-Probe Incorporated Adduct protection assay
US6017700A (en) * 1995-08-04 2000-01-25 Bayer Corporation Cationic oligonucleotides, and related methods of synthesis and use
US5837466A (en) 1996-12-16 1998-11-17 Vysis, Inc. Devices and methods for detecting nucleic acid analytes in samples
WO1998027209A1 (en) * 1996-12-18 1998-06-25 Emory University Polycationic oligomers
US5928869A (en) * 1997-05-30 1999-07-27 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
WO1999011813A2 (en) * 1997-09-04 1999-03-11 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
US6255476B1 (en) * 1999-02-22 2001-07-03 Pe Corporation (Ny) Methods and compositions for synthesis of labelled oligonucleotides and analogs on solid-supports
CA2270106C (en) * 1999-04-23 2006-03-14 Yousef Haj-Ahmad Nucleic acid purification and process
US6281005B1 (en) * 1999-05-14 2001-08-28 Copernicus Therapeutics, Inc. Automated nucleic acid compaction device
US6287774B1 (en) * 1999-05-21 2001-09-11 Caliper Technologies Corp. Assay methods and system
WO2001006011A2 (en) 1999-07-14 2001-01-25 Genometrix Genomics, Inc. Arrays comprising non-covalently associated nucleic acid probes and methods for making and using them
US6297016B1 (en) * 1999-10-08 2001-10-02 Applera Corporation Template-dependent ligation with PNA-DNA chimeric probes
US6380377B1 (en) * 2000-07-14 2002-04-30 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
US6448407B1 (en) * 2000-11-01 2002-09-10 Pe Corporation (Ny) Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis
DE60228100D1 (de) * 2001-08-24 2008-09-18 Nof Corp Verfahren zur beurteilung einer fehlpaarung zwischen einzelsträngigen nukleinsäuremolekülen
WO2007043450A1 (ja) * 2005-10-03 2007-04-19 Matsumura Oil Research Corp. パーフルオロポリエーテル化合物、およびこれを用いた潤滑剤ならびに磁気ディスク

Also Published As

Publication number Publication date
JP2004537263A (ja) 2004-12-16
DE60140317D1 (de) 2009-12-10
EP1377679B1 (de) 2009-10-28
AU2002230901B2 (en) 2006-09-07
US20020164614A1 (en) 2002-11-07
US20040077014A1 (en) 2004-04-22
CA2436857A1 (en) 2002-06-20
AU3090102A (en) 2002-06-24
EP1377679A2 (de) 2004-01-07
WO2002048404A3 (en) 2003-10-23
WO2002048404A2 (en) 2002-06-20
US7374945B2 (en) 2008-05-20

Similar Documents

Publication Publication Date Title
DE60140317D1 (de) Verfahren und kits zur verbesserung der hybridisierungsraten von polynukleotiden
WO2000024937A3 (en) Parallel methods for genomic analysis
DE69937108D1 (de) Verbindungen und Methoden zum Nachweis von Biomolekülen
ATE321088T1 (de) Polysiloxanpolymere, verfahren zu ihrer herstellung und ihre verwendung
EP1484416A3 (de) Methode zur Identifizierung und Einstufung spezifischer Fungi und Bakterien
ATE200694T1 (de) Verfahren, reagenzien und kits zur untersuchung von neisseria gonorrhoeae und chlamydia trachomatis
WO2002021098A3 (en) A method for identifying polymorphisms
DE60125243D1 (de) Polynukleotid-sequenzassay
WO1993019202A3 (en) Exchangeable template reaction
ATE230034T1 (de) Verfahren zur aufreinigung von dns
DE60332382D1 (de) Zentrifugalvorrichtung und verfahren zur durchführung von bindungsversuchen
DE69917606D1 (de) Herstellungsverfahren zur darstellung von zusammensetzungen, die anionische tonerden enthalten
ATE394507T1 (de) Verfahren zum nachweis von polynukleotiden
ES2175095T3 (es) Marcadores de adn respecto al tamaño de una camada de cerdos.
WO2005031004A3 (de) Verfahren zum spezifischen schnellnachweis getränkeschädlicher mikroorganismen
WO2001029262A3 (en) Genotyping reagents, kits and methods of use thereof
ATE466105T1 (de) Verfahren, zusammensetzungen und kits zur anreicherung und zum nachweis von mikroorganismen
DE60130970D1 (de) Reagenziensatz zur detektion von mikroorganismen, vorrichtung zur quantifizierungvon mikroorganismen und verfahren zur quantifizierung von mikroorganismen
CA2210769A1 (fr) Detection de bacteries du genre listeria selon des techniques d'hybridation de sondes nucleiques
DE60329929D1 (de) Molekulare identifizierung von bakterien des genus streptococcus und verwandte genus
DE69835376D1 (de) Verfahren zum auffinden von verbindungen zur vorbeugung von infektion oder pathogenizität
WO2002053771A3 (de) Nachweis von pathogenen bakterien
ATE340873T1 (de) Verfahren zur durchführung einer subtraktiven hybridisierung
DE60024679D1 (de) Methoden und zusammensetzungen zur detektion von spezies des mycobacterium avium-komplexes
ATE321308T1 (de) Verfahren zur auswahl von oligonukleotiden mit niedriger kreuzhybridisierung

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties