ATE448305T1 - Phagen-display mittels cotranslationaler translokation von fusionspolypeptiden - Google Patents

Phagen-display mittels cotranslationaler translokation von fusionspolypeptiden

Info

Publication number
ATE448305T1
ATE448305T1 AT06763985T AT06763985T ATE448305T1 AT E448305 T1 ATE448305 T1 AT E448305T1 AT 06763985 T AT06763985 T AT 06763985T AT 06763985 T AT06763985 T AT 06763985T AT E448305 T1 ATE448305 T1 AT E448305T1
Authority
AT
Austria
Prior art keywords
phage
polypeptides
phage display
particle
cotranslational translocation
Prior art date
Application number
AT06763985T
Other languages
English (en)
Inventor
Daniel Steiner
Patrik Forrer
Michael Stumpp
Andreas Plueckthun
Original Assignee
Univ Zuerich
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Zuerich filed Critical Univ Zuerich
Application granted granted Critical
Publication of ATE448305T1 publication Critical patent/ATE448305T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Control Of Indicators Other Than Cathode Ray Tubes (AREA)
AT06763985T 2005-07-08 2006-06-30 Phagen-display mittels cotranslationaler translokation von fusionspolypeptiden ATE448305T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05106236 2005-07-08
PCT/EP2006/063729 WO2007006665A1 (en) 2005-07-08 2006-06-30 Phage display using cotranslational translocation of fusion polypeptides

Publications (1)

Publication Number Publication Date
ATE448305T1 true ATE448305T1 (de) 2009-11-15

Family

ID=35063135

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06763985T ATE448305T1 (de) 2005-07-08 2006-06-30 Phagen-display mittels cotranslationaler translokation von fusionspolypeptiden

Country Status (20)

Country Link
US (2) US8846577B2 (de)
EP (1) EP1902131B1 (de)
JP (1) JP5042218B2 (de)
KR (1) KR101268939B1 (de)
CN (1) CN101213299B (de)
AT (1) ATE448305T1 (de)
AU (1) AU2006268780B2 (de)
BR (1) BRPI0613593A2 (de)
CA (1) CA2614512C (de)
DE (1) DE602006010386D1 (de)
DK (1) DK1902131T3 (de)
ES (1) ES2335136T3 (de)
IL (1) IL188518A (de)
MX (1) MX2008000345A (de)
NO (1) NO20080665L (de)
NZ (1) NZ564695A (de)
PL (1) PL1902131T3 (de)
RU (1) RU2412249C2 (de)
WO (1) WO2007006665A1 (de)
ZA (1) ZA200710687B (de)

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MX359570B (es) 2008-11-03 2018-10-01 Molecular Partners Ag Proteinas de union que inhiben la interaccion en el receptor de vegf-a.
GB0917647D0 (en) * 2009-10-08 2009-11-25 Glaxosmithkline Biolog Sa Expression system
SI2496601T1 (sl) 2009-11-05 2017-09-29 F. Hoffmann-La Roche Ag Tehnike in sestavek za sekrecijo heterolognih polipeptidov
AR081361A1 (es) 2010-04-30 2012-08-29 Molecular Partners Ag Proteinas de union modificadas que inhiben la interaccion de receptor del factor de crecimiento endotelial vascular de glicoproteina a vegf-a
CN113278077A (zh) 2010-11-26 2021-08-20 分子组合公司 设计的与血清白蛋白结合的重复蛋白
KR20140010076A (ko) 2011-02-15 2014-01-23 이뮤노젠 아이엔씨 세포독성 벤조다이아제핀 유도체
TW201302779A (zh) 2011-04-13 2013-01-16 Glaxosmithkline Biolog Sa 融合蛋白質及組合疫苗
WO2012149439A2 (en) 2011-04-29 2012-11-01 Janssen Biotech, Inc. Il4/il13 binding repeat proteins and uses
MX350954B (es) 2011-06-21 2017-09-26 Immunogen Inc Derivados de maitansinoides novedosos con enlazador de peptido y conjugados de estos.
EP2867360B1 (de) 2012-06-28 2019-05-08 Molecular Partners AG Von blutplättchen abstammender wachstumsfaktor (pdgf)-bindende, konstruierte ankyrin-repeat-proteine
US9956341B2 (en) 2012-07-03 2018-05-01 Milestone Scientific, Inc. Drug infusion with pressure sensing and non-continuous flow for identification of and injection into fluid-filled anatomic spaces
HK1211208A1 (zh) 2012-08-22 2016-05-20 Immunogen, Inc. 細胞毒性苯並二氮呯衍生物
EP2738180A1 (de) 2012-11-30 2014-06-04 Molecular Partners AG Bindeproteine mit mindestens zwei Bindedomänen gegen HER2
HK1219422A1 (zh) 2013-02-28 2017-04-07 Immunogen, Inc. 包含细胞结合剂及细胞毒素剂的轭合物
EP2961435B1 (de) 2013-02-28 2019-05-01 ImmunoGen, Inc. Konjugate mit zellbindenden mitteln und zytostatika
WO2014194030A2 (en) 2013-05-31 2014-12-04 Immunogen, Inc. Conjugates comprising cell-binding agents and cytotoxic agents
CN105209483B (zh) 2013-05-31 2021-07-27 分子组合公司 与肝细胞生长因子结合的设计锚蛋白重复蛋白
PL3116999T3 (pl) * 2014-03-14 2021-12-27 F.Hoffmann-La Roche Ag Sposoby i kompozycje do wydzielania polipeptydów heterologicznych
MX2016012285A (es) 2014-03-24 2017-01-23 Genentech Inc Tratamiento de cáncer con antagonista de c-met y correlación de estos con la expresión de hgf.
TW201613930A (en) 2014-09-03 2016-04-16 Immunogen Inc Cytotoxic benzodiazepine derivatives
PT3189056T (pt) 2014-09-03 2020-09-04 Immunogen Inc Derivados citotóxicos de benzodiazepina
BR112017020986A2 (pt) 2015-04-02 2018-08-14 Molecular Partners Ag. proteínas de ligação recombinantes e seu uso
US10220180B2 (en) 2015-10-16 2019-03-05 Milestone Scientific, Inc. Method and apparatus for performing a peripheral nerve block
EP3506917A4 (de) 2016-08-31 2020-05-06 President and Fellows of Harvard College Manipulierte bakterienproduzierende therapeutische proteine und verfahren zur verwendung davon
ES2953516T3 (es) 2016-09-22 2023-11-14 Molecular Partners Ag Proteínas de unión recombinantes y su uso
US10632255B2 (en) 2017-02-15 2020-04-28 Milestone Scientific, Inc. Drug infusion device
CN116785450A (zh) 2017-02-28 2023-09-22 伊缪诺金公司 具有自分解肽接头的类美登素衍生物和其缀合物
TW201839001A (zh) 2017-04-20 2018-11-01 美商伊繆諾金公司 細胞毒性苯并二氮平衍生物及其綴合物
US11471595B2 (en) 2017-05-04 2022-10-18 Milestone Scientific, Inc. Method and apparatus for performing a peripheral nerve block
EP3732178A1 (de) 2017-12-28 2020-11-04 ImmunoGen, Inc. Benzodiazepinderivate
AU2020240310A1 (en) 2019-03-21 2021-10-07 Immunogen, Inc. Methods of preparing cell-binding agent-drug conjugates
EP3947395A1 (de) 2019-03-29 2022-02-09 ImmunoGen, Inc. Zytotoxische bis-benzodiazepinderivate und konjugate davon mit zellbindenden mitteln zur hemmung von abnormalem zellwachstum oder zur behandlung von proliferativen krankheiten
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Also Published As

Publication number Publication date
CA2614512A1 (en) 2007-01-18
PL1902131T3 (pl) 2010-05-31
KR20080035603A (ko) 2008-04-23
IL188518A (en) 2011-04-28
JP5042218B2 (ja) 2012-10-03
BRPI0613593A2 (pt) 2011-01-18
EP1902131A1 (de) 2008-03-26
CN101213299B (zh) 2013-03-27
AU2006268780B2 (en) 2011-03-24
NO20080665L (no) 2008-02-05
ES2335136T3 (es) 2010-03-22
HK1122839A1 (en) 2009-05-29
CA2614512C (en) 2014-01-07
KR101268939B1 (ko) 2013-05-30
US20150057186A1 (en) 2015-02-26
WO2007006665A1 (en) 2007-01-18
MX2008000345A (es) 2008-04-02
RU2412249C2 (ru) 2011-02-20
NZ564695A (en) 2009-02-28
JP2009500027A (ja) 2009-01-08
US8846577B2 (en) 2014-09-30
RU2008104174A (ru) 2009-08-20
AU2006268780A1 (en) 2007-01-18
IL188518A0 (en) 2008-04-13
DK1902131T3 (da) 2010-03-01
DE602006010386D1 (en) 2009-12-24
ZA200710687B (en) 2008-11-26
EP1902131B1 (de) 2009-11-11
CN101213299A (zh) 2008-07-02
US20110118146A1 (en) 2011-05-19
US9447142B2 (en) 2016-09-20

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