ATE452137T1 - Verfahren und konstrukte zur expression von clostripain mit hoher ausbeute - Google Patents

Verfahren und konstrukte zur expression von clostripain mit hoher ausbeute

Info

Publication number
ATE452137T1
ATE452137T1 AT03734200T AT03734200T ATE452137T1 AT E452137 T1 ATE452137 T1 AT E452137T1 AT 03734200 T AT03734200 T AT 03734200T AT 03734200 T AT03734200 T AT 03734200T AT E452137 T1 ATE452137 T1 AT E452137T1
Authority
AT
Austria
Prior art keywords
clostripain
constructs
expressing
methods
high yield
Prior art date
Application number
AT03734200T
Other languages
English (en)
Inventor
Luan Peng
Yuannan Xia
Original Assignee
Restoragen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Restoragen Inc filed Critical Restoragen Inc
Application granted granted Critical
Publication of ATE452137T1 publication Critical patent/ATE452137T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Waveguides (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Drying Of Semiconductors (AREA)
AT03734200T 2002-05-24 2003-05-23 Verfahren und konstrukte zur expression von clostripain mit hoher ausbeute ATE452137T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38335702P 2002-05-24 2002-05-24
PCT/US2003/016642 WO2003100020A2 (en) 2002-05-24 2003-05-23 Methods and constructs for high yield expression of clostripain

Publications (1)

Publication Number Publication Date
ATE452137T1 true ATE452137T1 (de) 2010-01-15

Family

ID=29584550

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03734200T ATE452137T1 (de) 2002-05-24 2003-05-23 Verfahren und konstrukte zur expression von clostripain mit hoher ausbeute

Country Status (8)

Country Link
US (1) US8003362B2 (de)
EP (1) EP1521767B1 (de)
AT (1) ATE452137T1 (de)
AU (1) AU2003239894A1 (de)
CA (1) CA2485829A1 (de)
DE (1) DE60330564D1 (de)
DK (1) DK1521767T3 (de)
WO (1) WO2003100020A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
GB0426160D0 (en) * 2004-11-29 2004-12-29 Era Plantech S L Production of proteins
WO2010040847A2 (en) 2008-10-10 2010-04-15 Era Biotech, S.A. Recombinant protein bodies as immunogen-specific adjuvants
US8163880B2 (en) * 2006-02-23 2012-04-24 Era Biotech S.A. Production of biologically active proteins
KR101423697B1 (ko) 2012-07-27 2014-07-31 가톨릭대학교 산학협력단 인간 유전자의 세포 내 발현과 독성을 대장균에서 간단하고 빠르게 진단하기 위한 시스템
WO2017074257A1 (en) * 2015-10-26 2017-05-04 Nanyang Technological University Non-viable derivatives of clostridium sporogenes as anti-cancer therapeutic agents
WO2021046486A1 (en) * 2019-09-05 2021-03-11 Luckow Verne A Combinatorial assembly of composite arrays of site-specific synthetic transposons inserted into sequences comprising novel target sites in modular prokaryotic and eukaryotic vectors
CN113025599B (zh) * 2021-04-02 2023-09-12 重庆科润生物医药研发有限公司 一种重组溶组织梭菌i型胶原酶及其制备方法和应用

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4336336A (en) 1979-01-12 1982-06-22 President And Fellows Of Harvard College Fused gene and method of making and using same
US4551433A (en) 1981-05-18 1985-11-05 Genentech, Inc. Microbial hybrid promoters
US4876197A (en) 1983-02-22 1989-10-24 Chiron Corporation Eukaryotic regulatable transcription
US4588684A (en) 1983-04-26 1986-05-13 Chiron Corporation a-Factor and its processing signals
US4663280A (en) 1983-05-19 1987-05-05 Public Health Research Institute Of The City Of New York Expression and secretion vectors and method of constructing vectors
US4689406A (en) 1983-08-10 1987-08-25 Amgen Enhancement of microbial expression of polypeptides
US4880734A (en) 1984-05-11 1989-11-14 Chiron Corporation Eukaryotic regulatable transcription
US4738921A (en) 1984-09-27 1988-04-19 Eli Lilly And Company Derivative of the tryptophan operon for expression of fused gene products
US4745056A (en) 1984-10-23 1988-05-17 Biotechnica International, Inc. Streptomyces secretion vector
US4837148A (en) 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia
US4873192A (en) 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
US4929555A (en) 1987-10-19 1990-05-29 Phillips Petroleum Company Pichia transformation
BR8807310A (pt) 1987-11-18 1990-03-13 Chiron Corp Polinucleotidio,polinucleotidio recombinante,vetor recombinante,celula hospedeira,sistema de expressao recombinante,celula transformada,polipeptidio,hcv,preparacao de polipeptidio,polipeptidio de hcv,polipeptidio purificado,polipeptidio recombinante,polipeptidio de fusao,anticorpo monoclonal,preparacao purificada de anticorpos policlonais,particula,sonda de polinucleotidio,kit anticorpo para epetopo de hcv,metodo para producao de polipeptidio,metodo para deteccao de acidos nucleicos de hcv,imunoensaio para detectar antigeno de hcv,imunoensaio para detectar anticorpos dirigidos contra antigeno de hcv,vacina para tratamento de infeccao de hcv,celula,celula infectada com hcv,metodo para producao de anticorpos para hcv e metodo para isolar cdna derivado do genoma de um agente infeccioso nao identificado
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
US5191063A (en) * 1989-05-02 1993-03-02 University Of Medicine And Dentistry Of New Jersey Production of biologically active polypeptides by treatment with an exogenous peptide sequence
JPH04108378A (ja) * 1990-08-28 1992-04-09 Sumitomo Electric Ind Ltd ヒトパーフォリンと反応するモノクローナル抗体
DE4445891A1 (de) * 1994-12-22 1996-06-27 Boehringer Mannheim Gmbh Rekombinante Proteinase aus Clostridium histolyticum und ihre Verwendung zur Isolierung von Zellen und Zellverbänden
US5846774A (en) 1997-03-21 1998-12-08 Bionebraska, Inc. Chlorella virus promoters
EP1127170A4 (de) * 1998-11-05 2005-04-27 Univ Leland Stanford Junior Menschliche monoklonale antikörper gegen menschlichen pan-hepatitis c virus
US7186539B1 (en) * 2001-08-31 2007-03-06 Pharmacia & Upjohn Company Method for refolding enzymes

Also Published As

Publication number Publication date
WO2003100020A2 (en) 2003-12-04
US20050244924A1 (en) 2005-11-03
DE60330564D1 (de) 2010-01-28
EP1521767A2 (de) 2005-04-13
CA2485829A1 (en) 2003-12-04
US8003362B2 (en) 2011-08-23
EP1521767B1 (de) 2009-12-16
WO2003100020A3 (en) 2004-10-28
AU2003239894A8 (en) 2003-12-12
EP1521767A4 (de) 2006-04-19
DK1521767T3 (da) 2010-04-12
AU2003239894A1 (en) 2003-12-12

Similar Documents

Publication Publication Date Title
ATE521701T1 (de) Verbesserte konstrukte zur expression lysosomaler polypeptide
DE60142226D1 (de) Pilz transkriptionsaktivator zur verwendung in verfahren zur herstellung von polypeptiden
DE60227064D1 (de) Von rna polymerasen abstammende polypeptide und ihre verwendungen
DE59712890D1 (de) Neues gen für eine aminosäure-deacetylase mit spezifität für n-acetyl-l-phosphinothricin, ihre isolierung und verwendung
DE60033138D1 (de) Polypeptide mit galaktoseoxidaseaktivität und dafür kodierende nukleisäuren
ATE522548T1 (de) Verfahren zur massenproduktion der konstanten region von immunglobulin
WO2004067706A3 (en) Production of multimeric proteins
EA202190889A1 (ru) Клостридиальные нейротоксины, содержащие экзогенную петлю активации
MX2007002737A (es) Proteina transportadora para la introduccion de compuestos quimicos en celulas neuronales.
ATE414775T1 (de) Polypeptide mit prolyldipeptidylaminopeptidase- aktivität und dafür kodierende nukleinsäuren
ATE319818T1 (de) Polypeptide mit aminopeptidaseaktivität und für diese kodierende nukleinsäuren
PH12013500995A1 (en) Methods for increasing yield and fine chemical production in plants
DE10345772A1 (de) Verfahren zur Herstellung von 3-Methylamino-1-(thien-2-yl)-propan-1-ol
DE60043428D1 (de) POLYPEPTIDE MIT VERZWEIGUNGSENZYM-AKTIVITäT UND FüR DIESE KODIERENDE NUKLEINSäUREN
ATE448312T1 (de) Leitsequenzen zur verwendung bei der produktion von proteinen
DE60330564D1 (de) Verfahren und konstrukte zur expression von clostripain mit hoher ausbeute
ATE358725T1 (de) Polypeptide mit aminopeptidaseaktivität und dafür kodierende nukleinsäuren
PE20021098A1 (es) ANTICUERPOS ESPECIFICOS DE CD44v6
ATE492631T1 (de) Verwendung von mutationen zur verbesserung von aspergillus-phytasen
DE60334109D1 (de) Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit
DE60235985D1 (de) Ntb-a, ein oberflächenmolekül impliziert in der aktivität natürlicher killerzellen (nk)
DE69632963D1 (de) Mch3, eine apoptosis-protease, kodierende nukleinsäuren und methoden zur verwendung
DK1664102T3 (da) Isoleret photoprotein mtclytin, samt dets anvendelse
DK1379639T3 (da) Muse-glucuronyl-C5-epimerase, DNA der koder for samme, og anvendelser deraf
DE50305376D1 (de) Verfahren zur hydrolyse von carotinoidestern

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties