ATE469247T1 - Verfahren zum nachweis von egfr-mutationen in blutproben - Google Patents
Verfahren zum nachweis von egfr-mutationen in blutprobenInfo
- Publication number
- ATE469247T1 ATE469247T1 AT07787764T AT07787764T ATE469247T1 AT E469247 T1 ATE469247 T1 AT E469247T1 AT 07787764 T AT07787764 T AT 07787764T AT 07787764 T AT07787764 T AT 07787764T AT E469247 T1 ATE469247 T1 AT E469247T1
- Authority
- AT
- Austria
- Prior art keywords
- egfr mutations
- blood samples
- detecting egfr
- sample
- nucleic acid
- Prior art date
Links
- 230000035772 mutation Effects 0.000 title abstract 3
- 239000008280 blood Substances 0.000 title abstract 2
- 210000004369 blood Anatomy 0.000 title abstract 2
- 102000052116 epidermal growth factor receptor activity proteins Human genes 0.000 title abstract 2
- 108700015053 epidermal growth factor receptor activity proteins Proteins 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- YOHYSYJDKVYCJI-UHFFFAOYSA-N n-[3-[[6-[3-(trifluoromethyl)anilino]pyrimidin-4-yl]amino]phenyl]cyclopropanecarboxamide Chemical compound FC(F)(F)C1=CC=CC(NC=2N=CN=C(NC=3C=C(NC(=O)C4CC4)C=CC=3)C=2)=C1 YOHYSYJDKVYCJI-UHFFFAOYSA-N 0.000 title abstract 2
- 239000000523 sample Substances 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 101150039808 Egfr gene Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 108700021358 erbB-1 Genes Proteins 0.000 abstract 1
- 239000002853 nucleic acid probe Substances 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/173—Nucleic acid detection characterized by the use of physical, structural and functional properties staining/intercalating agent, e.g. ethidium bromide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP06117551A EP1881079A1 (de) | 2006-07-20 | 2006-07-20 | Verfahren zum Nachweis von EGFR Mutationen in Blutproben |
| PCT/EP2007/057510 WO2008009740A1 (en) | 2006-07-20 | 2007-07-20 | Method for the detection of egfr mutations in blood samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE469247T1 true ATE469247T1 (de) | 2010-06-15 |
Family
ID=37564107
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT07787764T ATE469247T1 (de) | 2006-07-20 | 2007-07-20 | Verfahren zum nachweis von egfr-mutationen in blutproben |
Country Status (18)
| Country | Link |
|---|---|
| US (2) | US8637245B2 (de) |
| EP (2) | EP1881079A1 (de) |
| JP (1) | JP2009544283A (de) |
| KR (1) | KR20090042263A (de) |
| CN (1) | CN101506385A (de) |
| AT (1) | ATE469247T1 (de) |
| AU (1) | AU2007275140B2 (de) |
| CA (1) | CA2658694A1 (de) |
| CY (1) | CY1110736T1 (de) |
| DE (1) | DE602007006809D1 (de) |
| DK (1) | DK2046985T3 (de) |
| ES (1) | ES2346589T3 (de) |
| HR (1) | HRP20100414T1 (de) |
| PL (1) | PL2046985T3 (de) |
| PT (1) | PT2046985E (de) |
| RS (1) | RS51428B (de) |
| SI (1) | SI2046985T1 (de) |
| WO (1) | WO2008009740A1 (de) |
Families Citing this family (47)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1851339B1 (de) | 2005-02-11 | 2016-05-18 | Memorial Sloan-Kettering Cancer Center | Verfahren und zusammensetzungen zum nachweis einer arzneistoffresistenten egfr-mutante |
| GB0714058D0 (en) * | 2007-07-19 | 2007-08-29 | Abertec Ltd | detection of methylation in nucleic acid sequences |
| US10512910B2 (en) | 2008-09-23 | 2019-12-24 | Bio-Rad Laboratories, Inc. | Droplet-based analysis method |
| US9194861B2 (en) * | 2009-09-02 | 2015-11-24 | Bio-Rad Laboratories, Inc. | Method of mixing fluids by coalescence of multiple emulsions |
| US9132394B2 (en) | 2008-09-23 | 2015-09-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
| US9399215B2 (en) | 2012-04-13 | 2016-07-26 | Bio-Rad Laboratories, Inc. | Sample holder with a well having a wicking promoter |
| US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
| WO2011120020A1 (en) | 2010-03-25 | 2011-09-29 | Quantalife, Inc. | Droplet transport system for detection |
| US12090480B2 (en) | 2008-09-23 | 2024-09-17 | Bio-Rad Laboratories, Inc. | Partition-based method of analysis |
| US9598725B2 (en) | 2010-03-02 | 2017-03-21 | Bio-Rad Laboratories, Inc. | Emulsion chemistry for encapsulated droplets |
| US9492797B2 (en) | 2008-09-23 | 2016-11-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
| US11130128B2 (en) | 2008-09-23 | 2021-09-28 | Bio-Rad Laboratories, Inc. | Detection method for a target nucleic acid |
| US9764322B2 (en) | 2008-09-23 | 2017-09-19 | Bio-Rad Laboratories, Inc. | System for generating droplets with pressure monitoring |
| CN102405402A (zh) | 2008-09-23 | 2012-04-04 | 阔达生命有限公司 | 基于液滴的测定系统 |
| US8633015B2 (en) * | 2008-09-23 | 2014-01-21 | Bio-Rad Laboratories, Inc. | Flow-based thermocycling system with thermoelectric cooler |
| US8709762B2 (en) | 2010-03-02 | 2014-04-29 | Bio-Rad Laboratories, Inc. | System for hot-start amplification via a multiple emulsion |
| US12162008B2 (en) | 2008-09-23 | 2024-12-10 | Bio-Rad Laboratories, Inc. | Partition-based method of analysis |
| US8951939B2 (en) | 2011-07-12 | 2015-02-10 | Bio-Rad Laboratories, Inc. | Digital assays with multiplexed detection of two or more targets in the same optical channel |
| US9417190B2 (en) | 2008-09-23 | 2016-08-16 | Bio-Rad Laboratories, Inc. | Calibrations and controls for droplet-based assays |
| DE102010025496A1 (de) | 2009-10-09 | 2011-04-21 | Universität Duisburg-Essen | Verfahren zur Detektion von Genveränderungen, insbesondere Mutationen |
| JP2013510564A (ja) | 2009-11-13 | 2013-03-28 | パンガエア ビオテック、ソシエダッド、リミターダ | 肺癌におけるチロシンキナーゼ阻害剤に対する応答を予測するための分子バイオマーカー |
| KR101819938B1 (ko) | 2010-02-26 | 2018-01-19 | 주식회사 파나진 | Ｐｎａ 기반의 실시간 ｐｃｒ 클램핑을 이용한 ｅｇｆｒ 돌연변이 검출 방법 및 키트 |
| EP2550528B1 (de) | 2010-03-25 | 2019-09-11 | Bio-Rad Laboratories, Inc. | Tropfenerzeugung für tropfenbasierte prüfungen |
| CA2767113A1 (en) | 2010-03-25 | 2011-09-29 | Bio-Rad Laboratories, Inc. | Detection system for droplet-based assays |
| WO2012002447A1 (ja) * | 2010-06-30 | 2012-01-05 | 武田薬品工業株式会社 | 変異ポリヌクレオチドの検出方法 |
| JP5860667B2 (ja) * | 2010-10-29 | 2016-02-16 | アークレイ株式会社 | Ｅｇｆｒエクソン２１ｌ８５８ｒ遺伝子多型検出用プライマーセット及びその用途 |
| JP5859274B2 (ja) * | 2010-10-29 | 2016-02-10 | アークレイ株式会社 | Ｅｇｆｒ遺伝子の多型検出用プローブ、増幅用プライマーおよびその用途 |
| CA2816712C (en) | 2010-11-01 | 2018-12-11 | Donald A. Masquelier | System for forming emulsions |
| EP2468883A1 (de) | 2010-12-22 | 2012-06-27 | Pangaea Biotech S.L. | Molekulare Biomarker zur Vorhersage der Reaktion auf Tyrosinkinaseinhibitoren bei Lungenkrebs |
| US12097495B2 (en) | 2011-02-18 | 2024-09-24 | Bio-Rad Laboratories, Inc. | Methods and compositions for detecting genetic material |
| EP2492688A1 (de) | 2011-02-23 | 2012-08-29 | Pangaea Biotech, S.A. | Molekulare Biomarker zur Vorhersage der Reaktion auf die Antitumorbehandlung bei Lungenkrebs |
| JP2014509865A (ja) | 2011-03-18 | 2014-04-24 | バイオ−ラッド・ラボラトリーズ・インコーポレーテッド | シグナルの組合せ使用による多重化デジタルアッセイ |
| EP2702175B1 (de) | 2011-04-25 | 2018-08-08 | Bio-Rad Laboratories, Inc. | Verfahren und zusammensetzungen zur nukleinsäureanalyse |
| WO2013019751A1 (en) | 2011-07-29 | 2013-02-07 | Bio-Rad Laboratories, Inc., | Library characterization by digital assay |
| WO2013190089A1 (en) | 2012-06-21 | 2013-12-27 | Pangaea Biotech, S.L. | Molecular biomarkers for predicting outcome in lung cancer |
| CN102732636B (zh) * | 2012-07-17 | 2014-01-15 | 海南医学院 | 一种检测肿瘤细胞egfr基因突变的芯片 |
| CN103923975B (zh) * | 2014-01-27 | 2016-01-20 | 上海涌泰生物医药科技有限公司 | 一种检测egfr基因外显子19缺失突变的试剂盒和方法 |
| CN103923974B (zh) * | 2014-01-27 | 2016-02-03 | 上海涌泰生物医药科技有限公司 | 一种检测egfr基因外显子20 t790m点突变的试剂盒和方法 |
| WO2015127234A1 (en) * | 2014-02-20 | 2015-08-27 | Board Of Regents, The University Of Texas System | Use of ibrutinib to treat egfr mutant cancer |
| CN103882137B (zh) * | 2014-04-03 | 2016-04-27 | 长沙三济生物科技有限公司 | 一种检测egfr基因858密码子突变的试剂盒及其应用 |
| CN104131091B (zh) * | 2014-07-21 | 2016-04-06 | 思博奥科生物信息科技（北京）有限公司 | 用于检测人类egfr基因突变的引物及方法 |
| US20170233818A1 (en) * | 2014-07-25 | 2017-08-17 | Panagene Inc. | Multiple target nucleic acid detection method using clamping probe and detection probe |
| CN105838777A (zh) * | 2015-01-13 | 2016-08-10 | 上海张江转化医学研发中心有限公司 | ddPCR技术监测肺癌患者对酪氨酸激酶抑制剂继发耐药的方法 |
| CN105886648A (zh) * | 2016-06-06 | 2016-08-24 | 武汉海吉力生物科技有限公司 | 用于检测egfr基因t790m突变的试剂盒 |
| CN107574233A (zh) * | 2017-10-25 | 2018-01-12 | 长沙三济生物科技有限公司 | 用于检测egfr基因19外显子缺失突变的引物组、试剂盒及方法 |
| CN108728536A (zh) * | 2018-05-25 | 2018-11-02 | 广州永诺生物科技有限公司 | 人egfr基因t790m位点检测的引物、探针、pcr反应液和试剂盒 |
| CN109554444A (zh) * | 2018-12-31 | 2019-04-02 | 新羿制造科技（北京）有限公司 | 一种检测核酸突变顺反式结构的方法及其试剂盒 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2564127T5 (es) * | 2004-06-04 | 2020-03-26 | Genentech Inc | Mutaciones en EGFR |
-
2006
- 2006-07-20 EP EP06117551A patent/EP1881079A1/de not_active Withdrawn
-
2007
- 2007-07-20 DE DE602007006809T patent/DE602007006809D1/de active Active
- 2007-07-20 ES ES07787764T patent/ES2346589T3/es active Active
- 2007-07-20 PT PT07787764T patent/PT2046985E/pt unknown
- 2007-07-20 RS RSP-2010/0363A patent/RS51428B/sr unknown
- 2007-07-20 PL PL07787764T patent/PL2046985T3/pl unknown
- 2007-07-20 AU AU2007275140A patent/AU2007275140B2/en not_active Ceased
- 2007-07-20 US US12/374,307 patent/US8637245B2/en active Active
- 2007-07-20 DK DK07787764.5T patent/DK2046985T3/da active
- 2007-07-20 CA CA002658694A patent/CA2658694A1/en not_active Abandoned
- 2007-07-20 EP EP07787764A patent/EP2046985B1/de active Active
- 2007-07-20 JP JP2009519991A patent/JP2009544283A/ja active Pending
- 2007-07-20 AT AT07787764T patent/ATE469247T1/de active
- 2007-07-20 HR HR20100414T patent/HRP20100414T1/hr unknown
- 2007-07-20 SI SI200730301T patent/SI2046985T1/sl unknown
- 2007-07-20 WO PCT/EP2007/057510 patent/WO2008009740A1/en not_active Ceased
- 2007-07-20 CN CNA2007800316040A patent/CN101506385A/zh active Pending
- 2007-07-20 KR KR1020097003360A patent/KR20090042263A/ko not_active Ceased
-
2010
- 2010-08-13 CY CY20101100756T patent/CY1110736T1/el unknown
-
2013
- 2013-12-21 US US14/138,013 patent/US20140113293A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20140113293A1 (en) | 2014-04-24 |
| DE602007006809D1 (de) | 2010-07-08 |
| RS51428B (sr) | 2011-04-30 |
| CN101506385A (zh) | 2009-08-12 |
| KR20090042263A (ko) | 2009-04-29 |
| AU2007275140B2 (en) | 2013-06-20 |
| JP2009544283A (ja) | 2009-12-17 |
| WO2008009740A1 (en) | 2008-01-24 |
| US20100009360A1 (en) | 2010-01-14 |
| DK2046985T3 (da) | 2010-09-27 |
| US8637245B2 (en) | 2014-01-28 |
| CY1110736T1 (el) | 2015-06-10 |
| PT2046985E (pt) | 2010-08-24 |
| PL2046985T3 (pl) | 2010-10-29 |
| HRP20100414T1 (hr) | 2010-09-30 |
| CA2658694A1 (en) | 2008-01-24 |
| AU2007275140A1 (en) | 2008-01-24 |
| EP1881079A1 (de) | 2008-01-23 |
| SI2046985T1 (sl) | 2010-09-30 |
| EP2046985A1 (de) | 2009-04-15 |
| HK1128497A1 (en) | 2009-10-30 |
| ES2346589T3 (es) | 2010-10-18 |
| EP2046985B1 (de) | 2010-05-26 |
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| UEP | Publication of translation of european patent specification |
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