ATE472101T1 - Assay zur bestimmung der aktivität von clostridien-toxin mittels kombination von fret und fluoreszenzpolarisation - Google Patents

Assay zur bestimmung der aktivität von clostridien-toxin mittels kombination von fret und fluoreszenzpolarisation

Info

Publication number
ATE472101T1
ATE472101T1 AT05858107T AT05858107T ATE472101T1 AT E472101 T1 ATE472101 T1 AT E472101T1 AT 05858107 T AT05858107 T AT 05858107T AT 05858107 T AT05858107 T AT 05858107T AT E472101 T1 ATE472101 T1 AT E472101T1
Authority
AT
Austria
Prior art keywords
clostridial toxin
fluorescence polarization
fret
assay
activity
Prior art date
Application number
AT05858107T
Other languages
English (en)
Inventor
Dudley Williams
Marcella Gilmore
Lance Steward
Marc Verhagen
Kei Aoki
Original Assignee
Allergan Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36074524&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE472101(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Allergan Inc filed Critical Allergan Inc
Application granted granted Critical
Publication of ATE472101T1 publication Critical patent/ATE472101T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Prostheses (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT05858107T 2004-09-22 2005-09-09 Assay zur bestimmung der aktivität von clostridien-toxin mittels kombination von fret und fluoreszenzpolarisation ATE472101T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/948,097 US7399607B2 (en) 2004-09-22 2004-09-22 Fluorescence polarization assays for determining clostridial toxin activity
PCT/US2005/032328 WO2007001358A2 (en) 2004-09-22 2005-09-09 Fluorescence polarization assays for determining clostridial toxin activity

Publications (1)

Publication Number Publication Date
ATE472101T1 true ATE472101T1 (de) 2010-07-15

Family

ID=36074524

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05858107T ATE472101T1 (de) 2004-09-22 2005-09-09 Assay zur bestimmung der aktivität von clostridien-toxin mittels kombination von fret und fluoreszenzpolarisation

Country Status (11)

Country Link
US (7) US7399607B2 (de)
EP (2) EP2107112A1 (de)
JP (2) JP2008513038A (de)
KR (1) KR101442246B1 (de)
AT (1) ATE472101T1 (de)
AU (1) AU2005333632B2 (de)
CA (1) CA2581231A1 (de)
DE (1) DE602005021981D1 (de)
DK (1) DK1807698T3 (de)
ES (2) ES2345780T3 (de)
WO (1) WO2007001358A2 (de)

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US8022172B2 (en) * 2001-08-28 2011-09-20 Allergan, Inc. Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity
US20080064054A1 (en) * 2001-08-28 2008-03-13 Ester Fernandez-Salas Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity
US7208285B2 (en) * 2001-08-28 2007-04-24 Allergan, Inc. Fret protease assays for botulinum serotype A/E toxins
US7183066B2 (en) * 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
US7399607B2 (en) * 2004-09-22 2008-07-15 Allergan, Inc. Fluorescence polarization assays for determining clostridial toxin activity
US8124357B2 (en) * 2005-04-05 2012-02-28 Allergan, Inc. Lipophilic dye-based fret assays for clostridal toxin activity
WO2008036060A2 (en) * 2005-04-05 2008-03-27 Allergan, Inc. Clostridial toxin activity assays
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WO2011041779A1 (en) 2009-10-02 2011-04-07 Allergan, Inc. In vitro method of determining changes in a protein environment
EP3279335B1 (de) 2011-12-31 2021-03-10 Allergan, Inc. Hochempfindlicher zellbasierter test für den nachweis der anwesenheit von aktivem botulinum-neurotoxin-serotyp-a
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US8962340B2 (en) 2012-12-03 2015-02-24 Src, Inc. Real-time assay for the detection of botulinum toxin
KR102349256B1 (ko) 2013-08-09 2022-01-07 바이오메디슨, 인코퍼레이티드 개선된 민감성을 지닌 보툴리눔 독소 검정
CA2954504A1 (en) 2014-07-07 2016-01-14 Allergan, Inc. Method of detecting cleaved snap25 in tissue samples
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
FR3053689B1 (fr) * 2016-07-06 2021-01-08 Univ Claude Bernard Lyon Polypeptides substrats des proteases de la famille des astacines et leurs utilisations
IL308091B1 (en) 2016-09-13 2026-01-01 Allergan Inc Protein-free stabilized Clostridium toxin preparations
EP3529371B1 (de) * 2016-10-21 2022-06-22 Toxogen GmbH Funktioneller detektionstest frei von antikörpern zur serotypisierung von botulinum-neurotoxinen
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Also Published As

Publication number Publication date
US7632655B2 (en) 2009-12-15
ES2345780T3 (es) 2010-10-01
US7638294B2 (en) 2009-12-29
US7635574B2 (en) 2009-12-22
WO2007001358A3 (en) 2007-06-28
KR20070055609A (ko) 2007-05-30
US20080293085A1 (en) 2008-11-27
EP1807698B1 (de) 2010-06-23
US20060063222A1 (en) 2006-03-23
ES2928863T3 (es) 2022-11-23
US20100081156A1 (en) 2010-04-01
WO2007001358A2 (en) 2007-01-04
EP2107112A1 (de) 2009-10-07
US20080293084A1 (en) 2008-11-27
CA2581231A1 (en) 2007-01-04
DK1807698T3 (da) 2010-09-20
AU2005333632A1 (en) 2007-01-04
US20080305510A1 (en) 2008-12-11
US20100081155A1 (en) 2010-04-01
EP1807698A2 (de) 2007-07-18
KR101442246B1 (ko) 2014-10-01
DE602005021981D1 (de) 2010-08-05
ES2928863T5 (en) 2025-11-25
AU2005333632B2 (en) 2011-12-22
US20080305509A1 (en) 2008-12-11
JP2008513038A (ja) 2008-05-01
US7674601B2 (en) 2010-03-09
JP2012210215A (ja) 2012-11-01
US7399607B2 (en) 2008-07-15

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