ATE484581T1 - Rna-polymerasen aus bacteriophagen phi6 - phi14 und deren anwendungen - Google Patents

Rna-polymerasen aus bacteriophagen phi6 - phi14 und deren anwendungen

Info

Publication number
ATE484581T1
ATE484581T1 AT00988824T AT00988824T ATE484581T1 AT E484581 T1 ATE484581 T1 AT E484581T1 AT 00988824 T AT00988824 T AT 00988824T AT 00988824 T AT00988824 T AT 00988824T AT E484581 T1 ATE484581 T1 AT E484581T1
Authority
AT
Austria
Prior art keywords
rna
polymerase
synthesis
primer
rna synthesis
Prior art date
Application number
AT00988824T
Other languages
English (en)
Inventor
Eugeny Makeyev
Dennis Bamford
Original Assignee
Rna Line Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rna Line Oy filed Critical Rna Line Oy
Application granted granted Critical
Publication of ATE484581T1 publication Critical patent/ATE484581T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/127—RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/119—RNA polymerase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT00988824T 1999-12-21 2000-12-21 Rna-polymerasen aus bacteriophagen phi6 - phi14 und deren anwendungen ATE484581T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI992751 1999-12-21
PCT/FI2000/001135 WO2001046396A1 (en) 1999-12-21 2000-12-21 Rna polymerases from bacteriophage phi 6-phi 14 and use thereof

Publications (1)

Publication Number Publication Date
ATE484581T1 true ATE484581T1 (de) 2010-10-15

Family

ID=8555790

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00988824T ATE484581T1 (de) 1999-12-21 2000-12-21 Rna-polymerasen aus bacteriophagen phi6 - phi14 und deren anwendungen

Country Status (11)

Country Link
US (1) US20030124559A1 (de)
EP (1) EP1242586B1 (de)
JP (1) JP2003517837A (de)
AT (1) ATE484581T1 (de)
AU (1) AU785036B2 (de)
CA (1) CA2395239A1 (de)
DE (1) DE60045107D1 (de)
DK (1) DK1242586T3 (de)
ES (1) ES2352173T3 (de)
NZ (1) NZ519679A (de)
WO (1) WO2001046396A1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080199915A1 (en) 2003-06-06 2008-08-21 Rna-Line Oy Methods and Kits For Mass Production Of Dsrna
FR2857977B1 (fr) * 2003-07-23 2007-10-05 Centre Nat Rech Scient Procede de production industrielle d'arn et systeme utile pour ladite production
US7323310B2 (en) 2004-08-31 2008-01-29 Qiagen North American Holdings, Inc. Methods and compositions for RNA amplification and detection using an RNA-dependent RNA-polymerase
US20110053226A1 (en) * 2008-06-13 2011-03-03 Riboxx Gmbh Method for enzymatic synthesis of chemically modified rna
JP2013507942A (ja) 2009-10-21 2013-03-07 リボックス・ゲーエムベーハー 熱安定性ｒｎａ依存性ｒｎａポリメラーゼを用いる、ｒｎａの指数関数的増幅のための方法
US20120208242A1 (en) 2009-10-21 2012-08-16 Riboxx Gmbh Method and RNA Reactor for Exponential Amplification of RNA
BR112017020690A2 (pt) 2015-03-30 2018-06-26 Greenlight Biosciences Inc produção livre de células de ácido ribonucleico
CA3020312A1 (en) * 2016-04-06 2017-10-12 Greenlight Biosciences, Inc. Cell-free production of ribonucleic acid
CN111363787B (zh) * 2020-04-14 2023-04-11 上海市计量测试技术研究院 一种检测双链rna的方法及其试剂盒与应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE910081A1 (en) * 1990-01-10 1991-07-17 Chiron Corp The use of DNA-dependent RNA polymerase transcripts as¹reporter molecules for signal amplification in nucleic acid¹hybridization assays
ATE266729T1 (de) * 1998-12-11 2004-05-15 Biomerieux Bv Rna polymerase mutanten mit erhöhter stabilität

Also Published As

Publication number Publication date
CA2395239A1 (en) 2001-06-28
EP1242586B1 (de) 2010-10-13
NZ519679A (en) 2005-03-24
JP2003517837A (ja) 2003-06-03
EP1242586A1 (de) 2002-09-25
WO2001046396A1 (en) 2001-06-28
DK1242586T3 (da) 2010-11-29
ES2352173T3 (es) 2011-02-16
DE60045107D1 (de) 2010-11-25
US20030124559A1 (en) 2003-07-03
AU2518101A (en) 2001-07-03
AU785036B2 (en) 2006-08-31

Similar Documents

Publication Publication Date Title
Mender et al. Telomerase repeated amplification protocol (TRAP)
ES2743050T5 (es) Composiciones y procedimientos para cuantificar una secuencia de ácido nucleico en una muestra
US11396672B2 (en) Composition and methods for detecting adenosine modifications
EP1456409A4 (de) Paralleles scoring von polymorphismen mittels amplifikation und fehlerkorrektur
DE60230672D1 (de) Nukleinsäureanreicherung
EP4328303A3 (de) Neue verwendung
CN106164261A (zh) 适用于高温核酸合成的新颖逆转录酶
DE60322880D1 (de) Einfache katalytische dna-biosensoren für ionen auf grundlage von farbveränderungen
DE60220025D1 (de) Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
ATE329061T1 (de) Verfahren zur reduzierung unspezifischer amplifikation bei pcr
KR102098710B1 (ko) 헤어핀 프로브 기반 등온 핵산 증폭을 이용한 표적핵산 검출 방법
ATE294855T1 (de) Rna polymerase mutanten mit erhöhter thermostabilität
ATE484581T1 (de) Rna-polymerasen aus bacteriophagen phi6 - phi14 und deren anwendungen
Kumari et al. Detection of G-quadruplex DNA using primer extension as a tool
JP2009524412A (ja) 核酸の混合物におけるｐｃｒによる突然変異核酸の示差的増幅
ATE403663T1 (de) Nukleinsäureamplifikation mit duplexzwischenprodukten
EP4219771A3 (de) Zusammensetzungen und verfahren für den multiplexnachweis viraler krankheitserreger in proben
Kimoto et al. Site‐specific functional labeling of nucleic acids by in vitro replication and transcription using unnatural base pair systems
Stephenson-Tsoris et al. Hepatitis delta virus genome RNA synthesis initiates at position 1646 with a Nontemplated Guanosine
KR20150028514A (ko) Ｒｎａ 합성에 대한 산화 그래핀 기반 신규한 형광 분석법
CN109897856B (zh) 一组特异性识别精胺的寡核苷酸适配体
US20260043073A1 (en) Ambient temperature nucleic acid amplification and detection
DE602004019059D1 (de) Methode zur linearen nicht-selektiven amplifikation von nukleinsäuren
CN120530204A (zh) 核酸检测方法和系统
ATE407223T1 (de) Methode zum nachweis von staphylococcus aureus

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties