DK2275556T3 - Hurtig fremgangsmåde til screening af translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionpartnere screenet derfra - Google Patents

Hurtig fremgangsmåde til screening af translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionpartnere screenet derfra

Info

Publication number
DK2275556T3
DK2275556T3 DK10009260T DK10009260T DK2275556T3 DK 2275556 T3 DK2275556 T3 DK 2275556T3 DK 10009260 T DK10009260 T DK 10009260T DK 10009260 T DK10009260 T DK 10009260T DK 2275556 T3 DK2275556 T3 DK 2275556T3
Authority
DK
Denmark
Prior art keywords
fusion partners
translational fusion
screened
recombinant proteins
screening
Prior art date
Application number
DK10009260T
Other languages
English (en)
Inventor
Jung-Hoon Sohn
Eui-Sung Choi
Jung-Hoon Bae
Eung-Suck Lee
Mi-Kyung Shin
Original Assignee
Korea Res Inst Of Bioscience
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020040003610A external-priority patent/KR100626753B1/ko
Priority claimed from KR1020040003957A external-priority patent/KR20050076139A/ko
Application filed by Korea Res Inst Of Bioscience filed Critical Korea Res Inst Of Bioscience
Application granted granted Critical
Publication of DK2275556T3 publication Critical patent/DK2275556T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DK10009260T 2004-01-17 2004-12-30 Hurtig fremgangsmåde til screening af translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionpartnere screenet derfra DK2275556T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020040003610A KR100626753B1 (ko) 2004-01-17 2004-01-17 재조합 단백질 생산용 맞춤형 단백질융합인자의 초고속선별 방법
KR1020040003957A KR20050076139A (ko) 2004-01-19 2004-01-19 단백질 분비 생산을 유도하는 단백질융합인자
EP04808645A EP1713930B1 (en) 2004-01-17 2004-12-30 Rapid screening method of translational fusion partners for producing recombinant proteins and translational fusion partners screened therefrom

Publications (1)

Publication Number Publication Date
DK2275556T3 true DK2275556T3 (da) 2013-07-08

Family

ID=34797866

Family Applications (2)

Application Number Title Priority Date Filing Date
DK04808645T DK1713930T3 (da) 2004-01-17 2004-12-30 Hurtig screeningsmetode for translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionspartnere screenet derfra
DK10009260T DK2275556T3 (da) 2004-01-17 2004-12-30 Hurtig fremgangsmåde til screening af translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionpartnere screenet derfra

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK04808645T DK1713930T3 (da) 2004-01-17 2004-12-30 Hurtig screeningsmetode for translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionspartnere screenet derfra

Country Status (7)

Country Link
US (2) US8673822B2 (da)
EP (2) EP2275556B1 (da)
JP (2) JP5148879B2 (da)
AT (1) ATE486943T1 (da)
DE (1) DE602004029936D1 (da)
DK (2) DK1713930T3 (da)
WO (1) WO2005068658A1 (da)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8673822B2 (en) 2004-01-17 2014-03-18 Korea Research Institute Of Bioscience And Biotechnology Rapid screening method of translational fusion partners for producing recombinant proteins and translational fusion partners screened therefrom
KR20070009269A (ko) * 2005-07-15 2007-01-18 한국생명공학연구원 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자
WO2010064748A1 (en) * 2008-12-04 2010-06-10 Korea Research Institute Of Bioscience And Biotechnology Screening of abundantly secreted proteins and their use as fusion partners for the production of recombinant proteins
US8986956B2 (en) 2010-11-04 2015-03-24 Korea Research Institute Of Bioscience And Biotechnology Method for producing human epidermal growth factor in large volume from yeast
MX375448B (es) * 2013-07-18 2025-03-06 Mannkind Corp Composiciones farmacéuticas en polvo seco estables al calor y métodos.
CN104694523A (zh) * 2015-03-21 2015-06-10 江南大学 一种精氨酸脱亚胺酶的胞外表达方法及其应用
US20250059561A1 (en) * 2021-12-23 2025-02-20 Helsingin Yliopisto Method for screening a signal peptide for efficient expression and secretion of a heterologous polypeptide in mammalian cells
US20250134961A1 (en) * 2023-10-31 2025-05-01 Chienyu Investment Co., Ltd. Oral formulation for activation of autologous stem cells

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JPS60501140A (ja) 1983-04-22 1985-07-25 アムジエン 酵母による外因性ポリペプチドの分泌
ES2061482T3 (es) * 1986-05-02 1994-12-16 Gist Brocades Nv Metodo para la identificacion de secuencias de señal secretora para sintesis de proteina extracelular en bacillus.
FR2648713B1 (fr) * 1989-06-26 1994-06-10 Commissariat Energie Atomique Proteines hybrides entre une enzyme extracytoplasmique et une autre proteine, leur procede de preparation, ainsi que leur application comme agent de diagnostic
US5212058A (en) * 1990-05-09 1993-05-18 Massachusetts Institute Of Technology Nucleic acid encoding ubiquitin-specific proteases
US5547871A (en) * 1993-01-25 1996-08-20 American Cyanamid Company Heterologous signal sequences for secretion of insect controlling proteins
US5536637A (en) * 1993-04-07 1996-07-16 Genetics Institute, Inc. Method of screening for cDNA encoding novel secreted mammalian proteins in yeast
US5563046A (en) * 1993-08-02 1996-10-08 Celtrix Pharmaceuticals, Inc. Fusion polypeptides and proteins
EP0662515B1 (en) * 1993-12-10 2000-11-15 Korea Institute Of Science And Technology Signal sequences for secretion of heterologous proteins from yeast
DE69636536T2 (de) * 1995-02-03 2007-10-25 Asahi Glass Co., Ltd. Sekretionssignal-Gen und dieses enthaltender Expressionsvektor
EP1170366A1 (en) 1996-04-24 2002-01-09 Genetics Institute, Inc. Secreted proteins obtained by using Yeast invertase gene as reporter system
WO1997040146A1 (en) 1996-04-24 1997-10-30 Genetics Institute, Inc. Yeast invertase gene as reporter system for isolating cytokines
US5952171A (en) * 1996-11-19 1999-09-14 Millennium Biotherapeutics, Inc. Method for identifying genes encoding secreted or membrane-associated proteins
US6136569A (en) * 1997-02-27 2000-10-24 Genentech, Inc. Method of creating a cDNA library enriched in signal sequences
US20060009633A9 (en) * 1997-11-13 2006-01-12 Genset, S.A. Complementary DNAs encoding proteins with signal peptides
US6548633B1 (en) * 1998-12-22 2003-04-15 Genset, S.A. Complementary DNA's encoding proteins with signal peptides
US6150098A (en) * 1998-02-20 2000-11-21 Amgen Inc. Methods for identifying novel secreted mammalian polypeptides
WO1999049028A1 (en) * 1998-03-23 1999-09-30 Genentech, Inc. Method of selection for genes encoding secreted and transmembrane proteins
US6753314B1 (en) * 1999-04-01 2004-06-22 Curagen Corporation Protein-protein complexes and methods of using same
WO2001000661A2 (en) * 1999-06-25 2001-01-04 Genset Carnitine carrier related protein-1
US7029842B2 (en) * 2000-04-07 2006-04-18 Novozymes A/S Signal sequence trapping
EP1276857B1 (en) * 2000-04-07 2008-06-11 Novozymes A/S Signal sequence trapping
US20020127557A1 (en) * 2001-03-09 2002-09-12 Ruoying Tan Method for identification of cDNAs encoding signal peptides
US7314974B2 (en) * 2002-02-21 2008-01-01 Monsanto Technology, Llc Expression of microbial proteins in plants for production of plants with improved properties
US8673822B2 (en) 2004-01-17 2014-03-18 Korea Research Institute Of Bioscience And Biotechnology Rapid screening method of translational fusion partners for producing recombinant proteins and translational fusion partners screened therefrom
KR20070009269A (ko) * 2005-07-15 2007-01-18 한국생명공학연구원 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자
WO2010064748A1 (en) * 2008-12-04 2010-06-10 Korea Research Institute Of Bioscience And Biotechnology Screening of abundantly secreted proteins and their use as fusion partners for the production of recombinant proteins

Also Published As

Publication number Publication date
US8673822B2 (en) 2014-03-18
DE602004029936D1 (de) 2010-12-16
EP2275556B1 (en) 2013-04-24
EP1713930A4 (en) 2007-02-28
ATE486943T1 (de) 2010-11-15
JP5148879B2 (ja) 2013-02-20
DK1713930T3 (da) 2011-01-17
JP5662363B2 (ja) 2015-01-28
US9012367B2 (en) 2015-04-21
WO2005068658A1 (en) 2005-07-28
EP1713930B1 (en) 2010-11-03
EP2275556A1 (en) 2011-01-19
EP1713930A1 (en) 2006-10-25
US20140220631A1 (en) 2014-08-07
US20070275385A1 (en) 2007-11-29
JP2012105675A (ja) 2012-06-07
JP2007517519A (ja) 2007-07-05

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