ATE501251T1 - Zellbibliothek - Google Patents

Zellbibliothek

Info

Publication number
ATE501251T1
ATE501251T1 AT02709979T AT02709979T ATE501251T1 AT E501251 T1 ATE501251 T1 AT E501251T1 AT 02709979 T AT02709979 T AT 02709979T AT 02709979 T AT02709979 T AT 02709979T AT E501251 T1 ATE501251 T1 AT E501251T1
Authority
AT
Austria
Prior art keywords
novel
libraries
host cells
cell library
discovery
Prior art date
Application number
AT02709979T
Other languages
English (en)
Inventor
Neil Goldsmith
Alexandra Sorensen
Soren Nielsen
Original Assignee
Evolva Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Ltd filed Critical Evolva Ltd
Application granted granted Critical
Publication of ATE501251T1 publication Critical patent/ATE501251T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Eye Examination Apparatus (AREA)
  • Holo Graphy (AREA)
  • Aerials With Secondary Devices (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT02709979T 2001-01-25 2002-01-25 Zellbibliothek ATE501251T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200100128 2001-01-25
DKPA200100679 2001-05-01
US30086301P 2001-06-27 2001-06-27
PCT/DK2002/000056 WO2002059297A2 (en) 2001-01-25 2002-01-25 A library of a collection of cells

Publications (1)

Publication Number Publication Date
ATE501251T1 true ATE501251T1 (de) 2011-03-15

Family

ID=43646216

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02709979T ATE501251T1 (de) 2001-01-25 2002-01-25 Zellbibliothek

Country Status (9)

Country Link
US (2) US7838287B2 (de)
EP (1) EP1356037B1 (de)
JP (2) JP2004525624A (de)
AT (1) ATE501251T1 (de)
AU (1) AU2002227883A1 (de)
CA (1) CA2474172C (de)
DE (1) DE60239386D1 (de)
DK (1) DK1356037T3 (de)
WO (1) WO2002059297A2 (de)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
ATE501251T1 (de) 2001-01-25 2011-03-15 Evolva Ltd Zellbibliothek
ATE412742T1 (de) * 2002-01-25 2008-11-15 Evolva Ag Screeningmethoden nach mehreren parametern und entwicklung von zellen, um kleine moleküle mit mehreren funktionalitäten zu produzieren
USRE47770E1 (en) 2002-07-18 2019-12-17 Merus N.V. Recombinant production of mixtures of antibodies
PT1523496E (pt) 2002-07-18 2011-09-29 Merus B V Produção de misturas de anticorpos de forma recombinante
ATE527366T1 (de) * 2002-08-01 2011-10-15 Evolva Ltd Verfahren zur mischung von vielen heterologen genen
US20100069614A1 (en) 2008-06-27 2010-03-18 Merus B.V. Antibody producing non-human mammals
CA2527694C (en) 2003-05-30 2015-07-14 Hendricus Renerus Jacobus Mattheus Hoogenboom Fab library for the preparation of anti vegf and anti rabies virus fabs
AU2005205412B2 (en) * 2004-01-20 2011-08-11 Merus N.V. Mixtures of binding proteins
CN101218352B (zh) 2005-03-18 2013-09-04 米克罗比亚公司 产油酵母和真菌中类胡萝卜素的产生
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi
RU2572826C2 (ru) 2008-12-02 2016-01-20 Чиралджен, Лтд. Способ синтеза модифицированных по атому фосфора нуклеиновых кислот
KR101153866B1 (ko) 2009-05-21 2012-06-27 대한민국 형질전환용 운반체, 이의 제조방법, 이를 이용한 형질전환방법 및 형질전환체
SG10201403841QA (en) 2009-07-06 2014-09-26 Ontorii Inc Novel nucleic acid prodrugs and methods of use thereof
WO2011146833A1 (en) 2010-05-20 2011-11-24 Evolva Inc. Method of producing isoprenoid compounds in yeast
NZ708078A (en) 2010-06-02 2017-01-27 Evolva Nutrition Inc Recombinant production of steviol glycosides
JP5868324B2 (ja) 2010-09-24 2016-02-24 株式会社Wave Life Sciences Japan 不斉補助基
CN107365339A (zh) 2011-07-19 2017-11-21 波涛生命科学有限公司 合成官能化核酸的方法
BR122021015508B1 (pt) 2011-08-08 2022-03-29 Evolva Sa Método para produzir um glicosídeo de esteviol alvo
CN107383197B (zh) 2012-04-20 2021-12-10 美勒斯公司 用于产生免疫球蛋白样分子的方法和手段
WO2013191950A2 (en) 2012-06-22 2013-12-27 Monsanto Technology Llc Unique modular vector design
WO2014010718A1 (ja) 2012-07-13 2014-01-16 株式会社新日本科学 キラル核酸アジュバント
RU2693381C2 (ru) 2012-07-13 2019-07-02 Уэйв Лайф Сайенсес Лтд. Асимметричная вспомогательная группа
EP2872147B1 (de) 2012-07-13 2022-12-21 Wave Life Sciences Ltd. Verfahren zur herstellung von chiralen oligonukleotiden
US20150293076A1 (en) * 2012-10-22 2015-10-15 Bolt Threads, Inc. Cellular Reprogramming for Product Optimization
MY190346A (en) 2013-02-06 2022-04-15 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
EP2954061B1 (de) 2013-02-11 2023-11-22 Evolva SA Effiziente herstellung von steviolglycosiden in rekombinanten wirtszellen
EP3046585B1 (de) 2013-09-17 2021-08-25 Bolt Threads, Inc. Verfahren und zusammensetzungen zur synthetisierung verbesserter seidenfasern
US9580758B2 (en) 2013-11-12 2017-02-28 Luc Montagnier System and method for the detection and treatment of infection by a microbial agent associated with HIV infection
JPWO2015108046A1 (ja) 2014-01-15 2017-03-23 株式会社新日本科学 抗アレルギー作用を有するキラル核酸アジュバンド及び抗アレルギー剤
EP3095459A4 (de) 2014-01-15 2017-08-23 Shin Nippon Biomedical Laboratories, Ltd. Chirales nukleinsäureadjuvans mit antitumorwirkung und antitumormittel
JPWO2015108047A1 (ja) 2014-01-15 2017-03-23 株式会社新日本科学 免疫誘導活性を有するキラル核酸アジュバンド及び免疫誘導活性剤
CA2936712A1 (en) 2014-01-16 2015-07-23 Meena Chiral design
JP2017528125A (ja) 2014-08-11 2017-09-28 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． 組み換え宿主におけるステビオールグリコシドの産生
WO2016038095A2 (en) 2014-09-09 2016-03-17 Evolva Sa Production of steviol glycosides in recombinant hosts
CN108337892B (zh) 2015-01-30 2022-06-24 埃沃尔瓦公司 在重组宿主中生产甜菊醇糖苷
CN115851470B (zh) 2015-03-16 2025-09-02 帝斯曼知识产权资产管理有限公司 Udp-糖基转移酶
WO2017025362A1 (en) 2015-08-07 2017-02-16 Evolva Sa Production of steviol glycosides in recombinant hosts
US10982249B2 (en) 2016-04-13 2021-04-20 Evolva Sa Production of steviol glycosides in recombinant hosts
CN109312378A (zh) 2016-05-16 2019-02-05 埃沃尔瓦公司 在重组宿主中产生甜菊醇糖苷
KR20190046993A (ko) 2016-09-14 2019-05-07 볼트 쓰레즈, 인크. 긴 균일한 재조합 단백질 섬유
EP3535406A1 (de) 2016-11-07 2019-09-11 Evolva SA Herstellung von steviolglycosiden in rekombinanten wirten
JP2025170455A (ja) * 2022-10-05 2025-11-19 株式会社Logomix 細胞ライブラリおよびその製造方法
JP7212982B1 (ja) 2022-10-05 2023-01-26 株式会社Logomix 細胞ライブラリおよびその製造方法
WO2024137877A1 (en) * 2022-12-21 2024-06-27 Clara Foods Co. Systems and methods for high yielding recombinant microorganisms and uses thereof

Family Cites Families (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1341302C (en) 1983-02-22 2001-10-09 Rae Lyn Burke Yeast expression systems with vectors having gapdh or pyk promoters and synthesis of foreign protein
AU3556789A (en) 1988-04-22 1989-11-24 Massachusetts Institute Of Technology Process for forming and using microdroplets
ATE196505T1 (de) 1989-07-07 2000-10-15 Unilever Nv Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopie-integrierung eines expressionsvektors tranformierten pilzes
US5316922A (en) * 1992-04-15 1994-05-31 The United States Of America As Represented By Department Of Health And Human Services Method for indentifying and expressing proteins that recognize and adhere to specific probes
US5482845A (en) 1993-09-24 1996-01-09 The Trustees Of Columbia University In The City Of New York Method for construction of normalized cDNA libraries
EP1650303B1 (de) 1993-10-27 2008-05-28 The Trustees of Columbia University of the City of New York Methode zur Bildung einer subtrahierten cDNA Bank und ihre Verwendung
US6268140B1 (en) 1994-02-03 2001-07-31 Neugenesis Combinatorial metabolic libraries
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5824485A (en) 1995-04-24 1998-10-20 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US6030779A (en) 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US6057103A (en) 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US6004788A (en) 1995-07-18 1999-12-21 Diversa Corporation Enzyme kits and libraries
US5958672A (en) 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US20010041333A1 (en) 1997-06-16 2001-11-15 Short Jay M. High throughput screening for a bioactivity or biomolecule
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
WO2000058517A1 (en) 1999-03-26 2000-10-05 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
GB9603803D0 (en) * 1996-02-22 1996-04-24 Zeneca Ltd Production of proteins, plasmids coding therefor and organisms containing such plasmids
US6025155A (en) * 1996-04-10 2000-02-15 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US6242211B1 (en) 1996-04-24 2001-06-05 Terragen Discovery, Inc. Methods for generating and screening novel metabolic pathways
WO1997044470A1 (en) 1996-05-21 1997-11-27 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
US5763239A (en) 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US6294325B1 (en) * 1996-07-05 2001-09-25 The Mount Sinai School Of Medicine Of The City University Of New York Cloning and expression of thermostable multi genes and proteins and uses thereof
AU6487196A (en) 1996-07-09 1998-02-02 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Transformation-associated recombination cloning
AU743305C (en) 1997-01-17 2006-03-30 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
DE69824174T2 (de) 1997-02-27 2005-07-14 Cellomics, Inc. Ein system zur zellbasierten reihenuntersuchung
WO1998041869A1 (en) 1997-03-18 1998-09-24 Chromaxome Corporation Methods for screening compounds using encapsulated cells
DE69732378D1 (de) 1997-05-27 2005-03-03 Hanil Synthetic Fiber Co Ltd Verfahren zur herstellung von rekombinanten eiweissen durch verwendung von hocheffizienten expressionsvektoren aus saccharomyces cerevisiae
GB9727512D0 (en) 1997-12-31 1998-02-25 Adprotech Plc Fuzzy genes and their application in molecular adjuvants
WO1999045154A1 (en) 1998-03-04 1999-09-10 Diversa Corporation Production and use of normalized dna libraries
AU5217599A (en) 1998-07-27 2000-02-21 Genotypes, Inc. Automatic eukaryotic artificial chromosome vector
WO2000008212A1 (en) 1998-08-07 2000-02-17 Cellay, Llc Gel microdrops in genetic analysis
WO2000012680A1 (en) 1998-08-31 2000-03-09 Maxygen, Inc. Transformation, selection, and screening of sequence-shuffled polynucleotides for development and optimization of plant phenotypes
AU6048599A (en) 1998-09-18 2000-04-10 Cellomics, Inc. A system for cell-based screening
EP1161529A2 (de) 1999-03-09 2001-12-12 Diversa Corporation Selektion am ende in gezielter evolution
GB9908814D0 (en) * 1999-04-16 1999-06-09 Celltech Therapeutics Ltd Process
AU7646000A (en) 1999-11-05 2001-05-14 Novozymes A/S Methods to screen microorganisms or gene libraries for products secreted from a cell
EP1276861A2 (de) * 2000-03-24 2003-01-22 Maxygen, Inc. Methoden zur modulation zellulärer und organismenspezifischer phenotypen
CA2474146C (en) 2001-01-25 2012-09-18 Evolva Ltd. A method for evolving a cell having desired phenotype and evolved cells
JP2004525623A (ja) 2001-01-25 2004-08-26 エボルバ バイオテック アクティーゼルスカブ ディファレンシャルに発現する同義遺伝子のコンカテマー
AU2002226307A1 (en) 2001-01-25 2002-08-06 Evolva Biotech A/S Artificial chromosomes comprising concatemers of expressible nucleotide sequences
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
ATE501251T1 (de) 2001-01-25 2011-03-15 Evolva Ltd Zellbibliothek
ATE412742T1 (de) 2002-01-25 2008-11-15 Evolva Ag Screeningmethoden nach mehreren parametern und entwicklung von zellen, um kleine moleküle mit mehreren funktionalitäten zu produzieren
DE60319259T2 (de) 2002-06-14 2009-03-05 Dyax Corp., Cambridge Rekombination von nukleinsäurebibliotheksmitgliedern
US20030235823A1 (en) 2002-06-24 2003-12-25 The University Of Alabama Nucleotide sequences that code for torsin genes, torsin proteins, and methods of using the same to treat protein-aggregation
ATE527366T1 (de) 2002-08-01 2011-10-15 Evolva Ltd Verfahren zur mischung von vielen heterologen genen

Also Published As

Publication number Publication date
JP2004525624A (ja) 2004-08-26
DK1356037T3 (da) 2011-06-27
DE60239386D1 (de) 2011-04-21
JP4965625B2 (ja) 2012-07-04
WO2002059297A8 (en) 2004-04-15
US7838287B2 (en) 2010-11-23
EP1356037B1 (de) 2011-03-09
JP2010043108A (ja) 2010-02-25
CA2474172C (en) 2012-07-10
US20040241672A1 (en) 2004-12-02
AU2002227883A1 (en) 2002-08-06
EP1356037A2 (de) 2003-10-29
US20120094870A1 (en) 2012-04-19
CA2474172A1 (en) 2002-08-01
WO2002059297A3 (en) 2003-04-10
WO2002059297A2 (en) 2002-08-01

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