ATE501268T1 - Vorrichtungen und verfahren zur bestimmung von proteaseaktivität - Google Patents

Vorrichtungen und verfahren zur bestimmung von proteaseaktivität

Info

Publication number
ATE501268T1
ATE501268T1 AT05852712T AT05852712T ATE501268T1 AT E501268 T1 ATE501268 T1 AT E501268T1 AT 05852712 T AT05852712 T AT 05852712T AT 05852712 T AT05852712 T AT 05852712T AT E501268 T1 ATE501268 T1 AT E501268T1
Authority
AT
Austria
Prior art keywords
protease activity
methods
devices
determining protease
determining
Prior art date
Application number
AT05852712T
Other languages
English (en)
Inventor
William Ward
Original Assignee
Univ Rutgers
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rutgers filed Critical Univ Rutgers
Application granted granted Critical
Publication of ATE501268T1 publication Critical patent/ATE501268T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT05852712T 2004-12-03 2005-12-01 Vorrichtungen und verfahren zur bestimmung von proteaseaktivität ATE501268T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US63293104P 2004-12-03 2004-12-03
US11/224,585 US7329506B2 (en) 2004-12-03 2005-09-12 Apparatuses and methods for determining protease activity
PCT/US2005/043572 WO2006060630A2 (en) 2004-12-03 2005-12-01 Apparatuses and methods for determining protease activity

Publications (1)

Publication Number Publication Date
ATE501268T1 true ATE501268T1 (de) 2011-03-15

Family

ID=36565750

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05852712T ATE501268T1 (de) 2004-12-03 2005-12-01 Vorrichtungen und verfahren zur bestimmung von proteaseaktivität

Country Status (6)

Country Link
US (2) US7329506B2 (de)
EP (1) EP1828404B1 (de)
JP (1) JP2008521442A (de)
AT (1) ATE501268T1 (de)
DE (1) DE602005026855D1 (de)
WO (1) WO2006060630A2 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7329506B2 (en) 2004-12-03 2008-02-12 Rutgers, The State University Apparatuses and methods for determining protease activity
US8673267B2 (en) 2009-03-02 2014-03-18 Massachusetts Institute Of Technology Methods and products for in vivo enzyme profiling
JP5645155B2 (ja) * 2009-08-31 2014-12-24 国立大学法人 琉球大学 分子量マーカー及び分子量マーカーの作製方法
US10006916B2 (en) 2011-03-15 2018-06-26 Massachusetts Institute Of Technology Multiplexed detection with isotope-coded reporters
EP3004374B1 (de) 2013-06-07 2020-08-12 Massachusetts Institute of Technology Affinitätsbasierter nachweis von ligandencodierten synthetischen biomarkern
EP3440013A4 (de) 2016-04-08 2021-03-17 Massachusetts Institute of Technology Verfahren zur spezifischen profilierung der protease-aktivität an lymphknoten
US11428689B2 (en) 2016-05-05 2022-08-30 Massachusetts Institute Of Technology Methods and uses for remotely triggered protease activity measurements
US11519905B2 (en) 2017-04-07 2022-12-06 Massachusetts Institute Of Technology Methods to spatially profile protease activity in tissue and sections
US11054428B2 (en) 2018-03-05 2021-07-06 Massachusetts Institute Of Technology Inhalable nanosensors with volatile reporters and uses thereof
US11732009B2 (en) 2018-06-08 2023-08-22 Glympse Bio, Inc. Activity sensor with tunable analyte
US11028425B2 (en) 2018-06-08 2021-06-08 Glympse Bio, Inc. Diagnosis and monitoring of liver disease
WO2020068920A2 (en) 2018-09-25 2020-04-02 Massachusetts Institute Of Technology Lung protease nanosensors and uses thereof
EP3911753A1 (de) 2019-01-17 2021-11-24 Massachusetts Institute of Technology Sensoren zur erfassung und abbildung von krebsmetastasen
CA3131900A1 (en) 2019-02-28 2020-09-24 Georgia Tech Research Corporation Compositions and methods for logic-gated profiling of biologic activity
JP2023542867A (ja) 2020-09-11 2023-10-12 グリンプス バイオ， インコーポレイテッド 疾患の検出／診断、病期分類、モニタリング及び処置のための生体外でのプロテアーゼ活性検出
WO2022147138A1 (en) * 2020-12-31 2022-07-07 /1Glympse Bio, Inc. Ex vivo detection of pathogens in body fluid samples

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010046668A1 (en) * 1994-10-21 2001-11-29 G.D. Searle & Co. Fluorescence polarization method for determining protease activity
DE19843873A1 (de) * 1998-09-25 2000-03-30 Univ Halle Wittenberg Verfahren zur Bestimmung von Proteaseaktivitäten auf Zelloberflächen
DE19941239A1 (de) * 1999-08-26 2001-03-01 Imb Inst Fuer Molekulare Biote Proteasesubstrat und seine Verwendung
US6680178B2 (en) * 2000-06-02 2004-01-20 The Regents Of The University Of California Profiling of protease specificity using combinatorial fluorogenic substrate libraries
DE10038382A1 (de) * 2000-08-07 2002-02-21 Direvo Biotech Ag Zweifarbiges fluorimetrisches Proteaseassay
GB0111459D0 (en) * 2001-05-10 2001-07-04 Isis Innovation Universal fluorescent sensors
JP2004537047A (ja) * 2001-06-14 2004-12-09 アナディーズ　ファーマスーティカルズ，アイエヌシー． 標的分子のリガンドをスクリーニングする方法
WO2004048935A2 (en) * 2002-11-22 2004-06-10 Marligen Biosciences, Inc. Detection of protease enzymes
US20050214890A1 (en) * 2003-11-26 2005-09-29 Zhiqun Tan Novel "Cleave-N-Read" system for protease activity assay and methods of use thereof
US7329506B2 (en) 2004-12-03 2008-02-12 Rutgers, The State University Apparatuses and methods for determining protease activity

Also Published As

Publication number Publication date
US7883863B2 (en) 2011-02-08
WO2006060630A3 (en) 2009-05-14
EP1828404A4 (de) 2010-03-03
EP1828404B1 (de) 2011-03-09
EP1828404A2 (de) 2007-09-05
WO2006060630A2 (en) 2006-06-08
JP2008521442A (ja) 2008-06-26
US20080241867A1 (en) 2008-10-02
US7329506B2 (en) 2008-02-12
US20060121552A1 (en) 2006-06-08
DE602005026855D1 (de) 2011-04-21

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