ATE503836T1 - Fermentative produktion von sialinsäure (neu5ac) mit hoher ausbeute - Google Patents

Fermentative produktion von sialinsäure (neu5ac) mit hoher ausbeute

Info

Publication number
ATE503836T1
ATE503836T1 AT07820804T AT07820804T ATE503836T1 AT E503836 T1 ATE503836 T1 AT E503836T1 AT 07820804 T AT07820804 T AT 07820804T AT 07820804 T AT07820804 T AT 07820804T AT E503836 T1 ATE503836 T1 AT E503836T1
Authority
AT
Austria
Prior art keywords
sialic acid
neu5ac
microorganism
high yield
fermentative production
Prior art date
Application number
AT07820804T
Other languages
English (en)
Inventor
Eric Samain
Original Assignee
Centre Nat Rech Scient
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre Nat Rech Scient filed Critical Centre Nat Rech Scient
Application granted granted Critical
Publication of ATE503836T1 publication Critical patent/ATE503836T1/de

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1085Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90Isomerases (5.)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT07820804T 2006-10-03 2007-10-02 Fermentative produktion von sialinsäure (neu5ac) mit hoher ausbeute ATE503836T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US84864506P 2006-10-03 2006-10-03
PCT/EP2007/060422 WO2008040717A2 (en) 2006-10-03 2007-10-02 High yield production of sialic acid (neu5ac) by fermentation

Publications (1)

Publication Number Publication Date
ATE503836T1 true ATE503836T1 (de) 2011-04-15

Family

ID=39154131

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07820804T ATE503836T1 (de) 2006-10-03 2007-10-02 Fermentative produktion von sialinsäure (neu5ac) mit hoher ausbeute

Country Status (7)

Country Link
US (1) US20110165626A1 (de)
EP (1) EP2076601B1 (de)
JP (1) JP2010505403A (de)
AT (1) ATE503836T1 (de)
CA (1) CA2665332A1 (de)
DE (1) DE602007013588D1 (de)
WO (1) WO2008040717A2 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101809143A (zh) 2006-09-26 2010-08-18 锡拉丘兹大学 用于增加唾液酸产量的代谢工程大肠杆菌
BR112012025364A2 (pt) * 2010-04-07 2015-09-22 Novartis Ag método para geração de partícula semelhante a vírus de parvovírus b19
WO2013019625A2 (en) * 2011-07-29 2013-02-07 The University Of Wyoming Methods of using a bacterial glcnac-6-p 2'-epimerase to promote sialylation of glycoconjugates
US9816107B2 (en) 2011-07-29 2017-11-14 The University Of Wyoming Transgenic insect cells comprising a bacterial GlcNAc-6-P 2′-epimerase
US9783838B2 (en) * 2011-11-07 2017-10-10 The Regents Of The University Of California PmST3 enzyme for chemoenzymatic synthesis of alpha-2-3-sialosides
ES2930286T3 (es) 2013-03-14 2022-12-09 Glycosyn LLC Microorganismos y métodos para producir oligosacáridos sialilados y que contienen N-acetilglucosamina
CN103602627B (zh) * 2013-11-25 2015-03-18 武汉中科光谷绿色生物技术有限公司 一种产n-乙酰神经氨酸大肠杆菌工程菌及其构建方法和应用
EP3149188A1 (de) 2014-05-27 2017-04-05 Universitetet I Tromsø - Norges Arktiske Universitet Verwendung einer n-acetylneuraminat-lyase aus dem bakterium aliivibrio salmonicida bei der herstellung von neuraminsäure und derivate davon
CN104628794B (zh) * 2015-03-10 2017-05-10 武汉中科光谷绿色生物技术有限公司 一种对微生物发酵法生产的n‑乙酰神经氨酸进行分离提纯的方法
CN107267577A (zh) * 2016-04-05 2017-10-20 孙镧 微生物发酵生产n‑乙酰‑d‑氨基葡萄糖和/或d‑氨基葡萄糖盐的方法
EP3473644A1 (de) 2017-10-17 2019-04-24 Jennewein Biotechnologie GmbH Fermentative herstellung von n-acetylneuraminsäure
EP3486326A1 (de) 2017-11-21 2019-05-22 Jennewein Biotechnologie GmbH Verfahren zur reinigung von n-acetylneuraminsäure aus einer fermentationsbrühe
FR3110176B1 (fr) 2020-05-12 2022-05-13 Institut Nat Des Sciences Appliquees De Toulouse Souche et procédé de production d’oligosaccharides
EP4192945A1 (de) * 2020-08-10 2023-06-14 Inbiose N.V. Zelluläre herstellung sialylierter di- und/oder oligosaccharide
US20240368651A1 (en) * 2020-08-10 2024-11-07 Inbiose N.V. Cellular production of di- and/or oligosaccharides
CN112342234B (zh) * 2020-10-27 2023-02-21 江南大学 一种调控n-乙酰神经氨酸产量提高的重组枯草芽孢杆菌
CN113249285A (zh) * 2021-05-13 2021-08-13 浙江工业大学 一种高产n-乙酰神经氨酸的重组枯草芽孢杆菌及其构建方法、应用
US20240409974A1 (en) * 2021-10-08 2024-12-12 Kyowa Hakko Bio Co., Ltd. Microorganism having ability to produce n-acetylneuraminic acid and/or n-acetylneuraminic acid-containing carbohydrate and method for producing n-acetylneuraminic acid and/or n-acetylneuraminic acid-containing carbohydrate using said microorganism
CN115125278B (zh) * 2022-08-25 2022-11-18 山东合成远景生物科技有限公司 一种生产聚唾液酸用的补料液及聚唾液酸制备方法
WO2025032496A1 (en) 2023-08-07 2025-02-13 Carbocode S.A Sialylation of glycosphingolipids
WO2025032497A1 (en) 2023-08-07 2025-02-13 Carbocode S.A. Sialylation method

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE193551T1 (de) 1991-03-18 2000-06-15 Scripps Research Inst Oligosaccharide als enzymsubstrate und - inhibitoren: verfahren und zusammensetzungen
US7329514B2 (en) 2002-02-28 2008-02-12 Kyowa Hakko Kogyo Co., Ltd. Process for producing n-acetylneuraminic acid
EP1730293A2 (de) * 2004-03-17 2006-12-13 Glycofi, Inc. Verfahren zur konstruktion eines cytidinmonophosphat-sialinsäure-synthesewegs in pilzen und hefen
WO2006034225A2 (en) * 2004-09-17 2006-03-30 Neose Technologies, Inc. Production of oligosaccharides by microorganisms

Also Published As

Publication number Publication date
WO2008040717A2 (en) 2008-04-10
EP2076601A2 (de) 2009-07-08
CA2665332A1 (en) 2008-04-10
JP2010505403A (ja) 2010-02-25
WO2008040717A3 (en) 2008-06-12
US20110165626A1 (en) 2011-07-07
EP2076601B1 (de) 2011-03-30
DE602007013588D1 (de) 2011-05-12

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